USER MANUAL
Contents
1. 5 C 35 C 2 3 UE 31 C 809 40 C 50 lt 4 5 kg 0 90 240 V 50 602. Version 1 01 Page 8 LabelGuard Microliter Cell 3 2 dsDNA ssDNA RNA ape Gugr Applications Cuvette Applications LabelGuard LabelGuard Applications Cuvette Applications Paramete
3. 1 AA min AA min x Parameter Screen Parameter Screen Kinetirs Parameters 1 Step 1 3 Functions AR FPP Step 2 4 Kinetics Step3 Wavelength Step4 _ Delay time 600 10 NR Ste
4. OD OD OD 10D 600 8 x 10 cells ml OD OD OD 2 0
5. NanoPhotometer NanoPhotometer LabelGuard Lz gt Lahel PPIications CUwEtte lications Furnctions RE 9 tilities USB NanoPhotometerM PC NanoPhotometerW NP SW100 PC
6. on off Version 1 01 Page 33 Functions 5 1 Abs T A T Parameter Screen Parameter Screen Single wavelength Parameters Step 1 3 Functions ET Step 2 1 Single Wavelength Step 3 Wavelength Mode Step4
7. Abs260 Abs280 Abs320 Abs 260 Abs 320 x Abs 260 Abs 320 Abs 280 Abs 320 Abs 260 Abs 320 Abs 230 Abs 320 NO REO Abs PURE NUCLEIC ACID POLY dAdT wave 260 0 Abs 0 700 0 50 wave 280 0 Abs 0 383 200 0 225 0 250 0 275 0 300 0 325 0 350 0 wavelength 260 nm 230 nm 260 nm 280 nm
8. C Step 21 Results screen Back Standards screen Page 29 LabelGuard cuvette applications Calibration Screen manual entry iuret Calibration 0 044 1dB 05 3 Ld D 44 03 ru PH 1 Me ee 0 4 ig HH HH le LA BaSck Results screen Standard FathlEngth Sample Fathlengrth Step 27 Options Farameters Frint r 1 Edit Sample Pathlsngth Samble Humber Saue hethod to Frint ea Calibration Screen manual entry
9. C Step 16 Results screen Back Standards screen 1 Calibration Screen replicates on Step 17 Blank Step 18 replicate C Step 19 Step 20 replicate
10. Version 1 01 Page 56 Accessories 9 LAR OR SAN RE MLE MER MDL EL IL 190 1100 nm full scan range 200 950 nm 0 7 to 5 ul with LabelGuard Microliter Cell with cuvettes up to 3 5 ml quartz or plastic Flat grating Automatic upon switch on onm 2 nm 1 nm Pulsed xenon lamp 1024 element CCD array 0 300 to 2 500A 0 to 199 T 0 005 Abs or 1 of the reading whichever is the greater 546 nm 0 003 Abs 0 to 0 5 Abs 0 007 Abs 0 5 1 0 Abs lt 1 at 220 nm and 340 nm using NaNO gt 0 01 Abs hour after 20 min warm up 340 nm 0 005 pk to pk 0 002 pms USB port standard Bluetooth option 260 x 390 x 100 mm lt 4 5 kg 90 250 V 50760 Hz Max 30 VA
11. Ao A dA R Step 15 Step 16 Options Step 17 Escape Functions Options 1 parameters screen 2 3 4 to dA J
12. The procedure is as follows Parameter Screen Concentration Farameters Fauvelength Ta Fathlength F adctor 2 mw Concentration Farameters Result Screen if using a Factor Concentration Version 1 01 Parameter Screen Step 1 3 Functions Step 2 2 Concentration Step 3 Wavelength Step 4 Factor Standard Mode Step5 Factor Factor 0 001 9999 C Step6 Standard 0 01 9999 C
13. Back Parameter screen LabelGuard cuvette applications Calibration Screen replicates off iuret Calibration Standards Di D400 GD Ls DH Hi 04 DE 09 LO Ja LA EBiuret Calibration 0 ee id ng aa Luo ua ld 2 mk Calibration Screen replicates on iuret Calibration Replicates 1 D 3d53 HH BU LA 0 n a 04 DG nH L0 la HH Version 1 01 Calibration Screen replicates off Step 13 Blank Step 14 C Q Step 15
14. C Step 22 Results screen Back Standards screen Page 20 LabelGuard cuvette applications Calibration Screen manual entry BCA Calibration 1 Hi H 1r 1 HGG Hg 4 H E1E 03 on HE ml n a 04 DG nH HH la LA B CE Results screen 0289 A Sitandard Fathlength Sample Fathlength Options 3 Parameters Print Graph Edit Sample Pathlength Samble Humber hiethod tn PFrint Version 1 01 Calibration Screen manual entry
15. C Step 17 Results screen Back Standards screen 1 Calibration Screen replicates On Step 18 Blank Step 19 replicate C Step 20 Step 21 replicate
16. 10 mm OD LabelGuard Version 1 01 Page 31 LabelGuard cuvette applications Parameter Screen DD EO Farameters Wavelength Cormectinn 2 DUD nits lt Cancel HH Gh Farameters ra wlEmngth F CImr EHH nm 1 nH Correction Multiplier 2 DDD nH 1 nits 9 oe H HHS Options 11 Farameters Frint Sample humber ah to Frint Version 1 01 Parameter Screen Step 1 2 Cuvette Applications Step 2 3 OD 600 Step 3 Wavelength 600 mm Step 4 _Correction
17. Cancel O Page 39 Functions Llsgr efined Feak Add Peak en button a D 3DO Parameters Print 7 Peak etection elete Peab Graph Scale Sample umber Saue hethod mio Print 6 Co nn Graph Scale Shortcut button 6 raph Scale rhom Hode Version 1 01 Add Peak 5 User Defined Peak Delete Peak Graph Scale 6 x y
18. e 12 Version 1 01 warranty Page 57 Specification and 10 10 1 260 nm Lambert Beer SE20CDOD cnuc A260 Factornuc Lid Factor Cnuc ng pl A260 AU Factornuc ng cm l ds DNA 50 ssDNA 37 RNA 40 Oligo 33 10 2 Lambert Beer 260nm
19. 0 001 to 9999 C OK Results screen Back Standards screen Results screen Step 22 Blank Step 23 Step 24 Step 25 Options Step 26 Protein Options 1 parameters screen 2 3 on off
20. Parameter Screen Parameter Screen bsnrbance Ratio wavelengths Step 1 3 Functions EE TE Step 2 7 Absorbance Ratio Step3 first Wavelength Wavelength 2 Step 4 seco7g Wavelength Step5 1 2 Background Step 6 On third Wavelength MM ca Step7 Next CancelO Functions Background Step 8 Pathlength LabelGuard applications 0 2 bsorbhance Ratin Farameters Pathlength 3 1 2 mm cuvette applications 5 mm 2 mm Dilution Factor Step9
21. Back Parameter screen LabelGuard cuvette applications Calibration Screen replicates off CA Calibration Hi 04 HE 08 LO Ja LA Ealibration tandards 0 200 0 051 0 400 DT7D ms 0 600 288 n_n 4 3 1 000 0516a 1 4nn E2E Ma 1 ee Dd HE DB Li Ja LAH 2 wm Calibration Screen replicates on a m He 0 4 0 6 Hi TH 1 1iH Version 1 01 Calibration Screen replicates off Step 14 Blank Step 15 C Step 16
22. LabelGuard AO cag7 7o7S Cuve7e Applications uc e c Acids Protein OD 600 DNA LabelGuard RNA LabelGuard LabelGuard Nucleic Acids Protein UV 280 nm LabelGuard Christian Warburg 750 nm LabelGuard 546 nm OD600 4 1 DNA RNA 10 mm 1 0 dsDNA 50 ug ml ssDNA 37 ug ml RNA 40 ug ml 260 nm
23. Bradford Farameters Calibration ms Step 9 standards Standards Replicates Replicates OFF 1 8 Step 10 Next Standards screen Cancel Protein Standards Screen Step 11 Standards Screen Bradford Srandards 0 001 9999 C Step 12 Next Calibration screen
24. 260 TO Christian Warberg Biochemisceh Zeitung 310 384 1941 mg ml 1 55 X Abs 280 0 76 X Abs 260 CD kN 3 Pe 280 nm nk 320 nm 260 280 nm 2 2 260 nm
25. Step 19 replicate C ES Step 20 Step 21 replicate Step 22 C Step 23 Results screen Back Standards screen Page 44 Functions Standard Curwe Calibration Calibration Manual entry Step 24 ed Step 25
26. Excel EMF CSV NanoPhotometer Bluetooth PC NanoPhotometer 2 2 e TM LabelGuard 10 mm e
27. 260 nm 280 nm 280 nm 260 nm 260 280 260 280 260 280 Abs260 0 1 230 nm
28. 0 2 5 1 98768 2 98768 OK Cancel Step7 Curve Fit Standard Curue Farameters LA 3 Case Step 8 Calibration standards Regression k manual new standards Calibration Replicates Step9 standards ET OFF 1 2 3 Step 10 Next
29. Step 2 0 7 4 Hl 0 2 mm 3 5 ul 1 mm SN Step3 Step 4 Step5
30. 2 Step 5 Units OD cells ml cells ml 2 Step6 cells ml Factor 0 001 to 9999 C Step 7 cells ml Multiplier 1000 1 000 000 Step 8 OK Cancel Cuvette Applications Results Screen Step 7 Blank 9 Step 8 OD600 Step 9 Step 10 Options
31. 1 4 1 2 Step 18 Options Step 19 Func ons Options select using key pad numbers Options dh 1 Darameters Screen Ee 2 ee 3 7 SampleNumber 8 User Saue Method Methods 1 9 Auto Frint 9 Auto print auto print on off options
32. Step 12 Options Step 13 Functions Page 46 Functions Options Select using key pad numbers 11 Faameters Frint Frimt r nhl umber h ta Frint Version 1 01 Options 1 parameters screen 2 3 8 9 User Methods 1 9 10 Auto print auto print on off options Page 47 Functions 5 7 2
33. e e se ISO 9001 e e 8 2 8 3
34. 8 User Methods 1 9 9 Auto print auto print on off options Page 27 LabelGuard cuvette applications 4 2 5 Parameter Screen iuret Farameters Standards Fathlength EBiuret Farameters Calibration Standards Replicates Standards Screen Biuret Standards Std 4 HH a d Version 1 01 Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 LabelGuard LabelGuard Parameter Screen LabelGuard 1 Cuvette 2 2 Protein 4 Lowry
35. user mode minkm mi 1 udoku Setup Ta Computer k mm 2 wm Options Options select using key pad numbers 1 set up 3 4 8 Methods 1 9 J Escape Utilities Version 1 0 Page 55 Utilities 8 e Didymium NP HDF100 e NanoPhotometerTM NP SW100 e NanoPhotometer B 80 3003 84 e 5 VI G1D 04712 s Bluetooth B 80 3003 96 e NanoPhotometer W B 80 3004 02 8 1 GLP GMP
36. 33 pg ml 10 1 dsDNA ssDNA RNA 50 37 40 33 10 mm 260 280 DNA RNA gt 1 8 gt 2 0
37. Grid History Step 4 Standby 1 2 off Step 5 OK Utilities Cancel Utilities 7 5 Step 1 Contrast Step 2 Brightness Step 3 OK Utilitfes Conitrast Brightiness 7 6 Method Step 1 Folder Folder Hames Step 2
38. BCA bicinchoninic acid BCA 562nm DCT Folin Ciocalteu 750 nm BSA 280 nm BCA LabelGuard BSI 4
39. 0 2 mm 1 mm 1 50 1710 i LabelGuard 190 1100 nm LabelGuard 0 2 mm 1 mm 2 LabelGuard 15 mm 3 1 TM Step1 Insert the LabelGuard
40. Step 14 Blank Step 15 C Step 16 Step 17 C Ok Results screen Back Standards screen Calibration Screen replicates On Step 18 Blank
41. Step 26 OK Results screen Back Standards screen Results screen Step 23 Blank Step 24 Step 25 Step 26 Options Step 27 Protein Options 1 parameters screen 2 3 on off 230 260 280 320 nm 220 nm 750 nm J 4
42. amo nmoa A2607A280 1 99 1 545 Step 12 Step 13 Options 2607A230 ume 2 899 Step 14 Nucleic Acids Options Options 1 parameters screen Frint 2 3 on off 230 260 280 Print ata nl 320 nm 220 nm 750 nm A ample umber 4 on off Saue hiethod 7 uto FPrint
43. C Step 21 Results screen Back Standards screen Page 26 LabelGuard cuvette applications 1Y amp LU5E Ln 4 mn132A 1 A nm 0 J10 0 05 n a 04 Hi Hi 1in Hi HH EF Back Results screen h2 Standard Farhlength Sample Fathlength Options 1 Farameters Print Graph 1 Edit ampls Fathlength la Humber Saue hiethoad to Frint Version 1 01 Calibration Screen manual entry 0 001 to 9999 C OKW
44. Back Std 3 Std 6 Parameter screen Version 1 01 Page 22 LabelGuard cuvette applications Calibration Screen replicates off Bradford Calibration Calibration Screen replicates off a Step 13 Blank Step 14 C Hi 04 06 DB LO Le Ld 6 Step 15 Bradford Calibration ne 1 C NI
45. Step7 On 7s 8 Options hg ml ug pl pmolul mg dI mmoM umoM g l mg ug U ppm ppb conc OK DP 0 2 5 1 98768 2 98768 OK Cancel Step8 Pathlength LabelGuard applications 0 2 1 2 mm cuvette applications 5 mm mm Step 9 OK results screen CancelO Functions Result Scr
46. 230 nm Version 1 01 Page 10 LabelGuard cuvette applications Tris EDTA RNA 2607230 gt 2 0 RNA 230 260 nm RNA 2607280 260 230 10 mm 260 280 nm 320 nm
47. C Step 21 Results screen Back Standards screen Version 1 01 Page 23 LabelGuard cuvette applications Calibration Screen manual entry Bradford Calibration lat 13 hig 33 ES L719 ee 04 HE HH 1ih Je 1H BaSck lt K Results screen EBradford Standard Fathlength Sample Fathlength Options Farameters Frint r h E dit sample Pathlensth Gamole umber ethod to Print eae a Version 1 01 Calibration Screen manual entry 0 001 to 9999 C
48. Step 10 Result Screen T GA Absorbance T Step 11 Options Step 12 Escape Functions Page 38 Functions Options Faameters Print bs T Peak etection P Scal ample umber aue hiethod to Print Peak Detection Shortcut button 4 Peak Uetection
49. 2 1 2 0 001 to 9999 C EE ok S ss Step 26 OK Results screen Back Standards screen Results screen Step 27 Blank 4 Step 28 Step 29 Step 30 Options A Step 31 Functions Options Farameters 1 parameters screen Print 2 Graph 3
50. Step 11 Cuvette Applications Options 1 parameters screen 2 7 8 User Methods 1 9 9 Auto print auto print on off options Page 32 LabelGuard cuvette applications 5 Functions 11 Single auelength 2 200 950 nm 5 T 2
51. Back Parameter screen LabelGuard cuvette applications Calibration Screen replicates off Lowry Calibration Hi 04 DE 08 LO Ja LA Lowryr Calibration HE HU HH1r 0 400 LU5E 0 15 Eh D D35 _ 138 0 1L0 1 0D0D0 1 1 400 2 0 05 He 0 4 DG 0 8 LA Lg LH 2 ck a id ng DE LO la 1 Version 1 01 Calibration Screen replicates off Step 13 Blank Step 14 C Step 15
52. Results screen Back Standards screen Results screen Step 22 Blank Step 23 Step 24 Step 25 Options Step 26 Protein Options 1 parameters screen 2 3 on off 230 260 280 320 nm 220 nm 750 nm 4 7
53. Step 17 Options Step 18 Func ons Options 1 parameters screen 2 3 20nm on off 4 Run Standard screen 7 8 User Methods 1 9 9 Auto print auto print on off options Page 37 Functions 5 3 Parameter Screen Wascan Farameters lart rauelemngth Fathlength End avelength unTa lt OE Cancel Measurement Screen
54. C Step 16 Results screen Back Standards screen Calibration Screen replicates on Step 17 Blank Step 18 replicate C Step 19 Step 20 replicate
55. Dilution factor 1 00 9999 nits 2 Step 10 Options Vo u7ne 0 01 9999 Vokuma Diluent 0 01 9999 Step 11 OK Diluent Parameters screen Cancel Step 12 g ml ng pl pg pl a Step 13 0 001 9999 Step 14 OK results screen Cancel Functions Version 1 01 Page 48 Functions Result Screen Results Screen Absorbance Ratio Step 15 Blank a Step 16 Fate Step 17
56. 320 nm DNA RNA 4 Version 1 01 Page 11 LabelGuard cuvette applications 4 1 1 dsDNA ssDNA RNA Parameter Screen Step 1 LabelGuard 1 Cuvette 2 Step 2 1 Nucleic Acids EN Se Step 3 dsDNA 1 ssDNA 2 MA 3 Step 4 Pathlength Fathlength nits TM 5 mm 10 mm LabelGuard 3 2 Lid Dilution F CInr F CInr Facior Step 5 Dilution Factor 1 00 9999 C
57. 0 280 nm 2 0 00 0 1 BSA 1 1 115 0 0 8 mgml SS mg ml 1 115 x Abs 280 ss HiTrap 595 546 562 750 nm 6 595 BSA ie 546nm
58. 5 mm 10 mm LabelGuard 3 2 Lid Factor 2 Hl Bradford Parameters Step7 Units 8 Options hg ml ug pl pmol ul mg dI mmoMl umoM g l mg ug U ppm ppb conc OK DP 0 2 5 1 98768 2 98768 OK Cancel Next Step 8 standards manual new standards
59. 220 nm 750 nm 2 4 on off 7 8 User Methods 1 9 9 Auto print auto print on off options Page 14 LabelGuard cuvette applications 4 1 3 dsDNA ssDNA RNA dsDNA ssDNA RNA dsDNA ssDNA RNA
60. J Sample Enter a Selection Back Version 1 01 Page 6 Keypad and display Options Options Farameters Print Graph 2 Frint Data lp 3 4 v 7 TTT 8 Saue hethod to Print 9 On off off Escape Version 1 01 Page 7 Keypad and display 3 LABELGUARD i 0 7 5
61. 0 00 1 0 95 1 00 Version 1 01 Page 17 LabelGuard cuvette applications 4 2 1 UV Warburg Christian Parameter Screen Frntein UY Farameters Fathlength ED Factor Dilution Factor HH Factor 1 1 55 Eackground Units es Results Screen BS 1Er EMI Options Farameters Frint nl umber to Frint Version 1 01 Parameter Screen Step 1 LabelGuard 1 Cuvette 2 Step 2 2 Protein Step3 1 Protein Step4 Pathlength
62. Version 1 01 Page 49 FunctionSs 6 Options 9 User Methods 11 SEE ethods 1 Methods 2 J ER i hd ethods 3 ee lethods 4 a a Miethods 5 me ethods E J a 3 Methods 7 alL ee HE hethods 8 SL a EF Methods 3 SL Options Options 6 Folder Names Utilities Methnds Methods 1 ol ingle waelength 11 elete hethod Lock hethod EE nock Method Version 1 01
63. 8 User Methods 1 9 9 Auto print auto print on off options Version 1 01 Page 12 LabelGuard cuvette applications 4 1 2 Parameter Screen Hi dn Farameters Fathlength nits 1 Diluktion Factor 1 Dob Hackground ly EF 1 1 Results Screen Version 1 01 Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Step 12 Step 13 Parameter Screen LabelGuard 1 Cuvette 2 1 Auc e c Ac 9s 4 O go Pathlength 5 mm 10 mm LabelGuard 3 2 Lia Factor Dilution Factor
64. CancelW Next standards manual new standards Calibration standards Replicates OFF 1 2 3 Step 10 Next Standards screen Step 11 Cancel Protein Standards Screen 0 001 9999 C Step 12 Next Calibration screen Page 28
65. Zoom mode x amp y axis x y Iimits on X y axis limits x y axis limits On Cancel Page 40 Functions 5 4 Kinetics 340 nm NAD NADH
66. 1 5 1 0 1 5 1 HH 4 E 5DD nm ample Result Screen Version 1 01 Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Parameter Screen 3 Functions 3 Wavescan Start Wavelength End Wavelength Apso ace Transmission Mode Pathlength LabelGuard applications 0 2 1 2 mm cuvette applications 5 mm 2 mm OK measurements screen CancelO Functions Measurement Screen Blank
67. 220 nm 750 nm 8 User Methods 1 9 9 Auto print auto print on off Page 21 options LabelGuard cuvette applications 4 2 3 Parameter Screen Parameter Screen Step 1 LabelGuard 1 Cuvette Bradrord Farameters 2 TH Step 2 2 Protein Step 3 3 Bradford Step 4 Wavelength 595 mm Step5 EE Standard 1 9 5 Step6 Pathlength
68. 1 00 9999 C Options 0 01 9999 Parameter Screen Cancel Parameter Screen 320 nm Background Units pg ml ng ul ug ul pmolul pmoMul Factor 4 Factor 33 0 01 9999 10 0 9999 OK Results screen Cancel Muc ejc Acid
69. 320 nm 220 nm 750 nm 4 on off 7 8 User Methods 1 9 9 Auto print auto print on off options Page 16 LabelGuard cuvette applications 4 2 280 nm 280 nm Abs 280 My 280 nm
70. Wavelength 595 mm Standard 1 9 Pathlength 5 mm 10 mm LabelGuard 3 2 Lid Factor 2 Units 8 Options ug ml ug l pmol ul mg dI mmoMl umol l g l mg ug U ppm ppb conc OK 0 2 5 1 98768 2 98768 OK
71. 15 mm 9 LebelGuard 0 7 ul s 12 mm Version 1 0 Page 5 Introduction 2 3 LCD Display On off key lt lt Alphanumeric keys Cellholder fo en CH nm a Io wu EscaperCance Back Arrow keys BlankJReference View options Sample En
72. 7 User Methods 1 9 9 Auto print auto print on off options 8 Page 24 LabelGuard cuvette applications 4 2 4 Parameter Screen Lowry Farameters nits Lowry Farameters Calibration Standards k Replicates Standards Screen Lowry Standards Fs Tn E i a ea a H 1 DoOnb bi a es i a a EE 1 4 Version 1 01 Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Parameter Screen LabelGuard 1 Cuvette 2 2 Protein 4 Lowry Wavelength 595 mm S
73. 230 260 280 320 nm 220 nm 750 nm J 4 7 8 User Methods 1 9 9 Auto print auto print on off options Page 30 LabelGuard cuvette applications Version 1 01 4 3 OD600 600 nm OD600 0 4 OD600 0 600 OD
74. em Nm Options 0 01 9999 Parameter Screen lt Cancel Cancel Parameter Screen Step6 320 nm Background Step 7 Units LO ml ng pl pg HlI Step 8 Factor dsDNA 50 ssDNA 37 RNA 40 0 01 9999 Step 9 OK Results screen Cancel Results Screen uc e c Acids Results Screen Step 10 Blank Azao oomsa asoo oe Step 11
75. 5 1 98768 2 98768 OK Cancel Step 10 Factor 0 01 9999 Step 11 Pathlength LabelGuard applications 0 2 1 2 mm cuvette applications 5 mm 2 mm Step 12 Next results screen Cancel Parameters screen Version 1 01 Page 41 FunctionSs Result Screen 1 We nn 4 DE ns 1 0 Hole le sss oa ooos oo sos oom nnl 1 Time HH HH 1 bs SE Options Farameters Frint Print ata Sat tt et km mt LUTSDr Slope amble umber lthH to Print ol Tn nT a le nnn mn mm Version 1 01 Results Screen Step 13 Blank Step 14
76. 2 0 00 Parameter Screen Parameter Screen Standard Curve Farameters Step 1 3 Functions ET Step 2 5 Standard Curve Step3 Wavelength Standards Step4 1 9 Standard SS Ti Step5 Pathlength LabelGuard applications 0 2 1 2 mm cuvette applications 5 mm 2 mm Step6 _Units 8 late toa Etisal Options hg ml ug pl pmolul mg dI mmol l umoMl g l mg ug U 6 ppm ppb conc OK
77. cnuc A260 Cf 260 Amax Factornuc Lid factor Cnuc ng pl A260 AU Cf 260 260 nm Amax AU Factornuc ng cml ds DNA 50 ssDNA 37 RNA 40 Oligo 33 cdye Amax Lid factor Eaye 10exp 6 Cdye pmoul Amax AU Edye upmol cm e 1 000 FOD Formula for dsDNA FOl 6 49 Amax Eaye 10exp 6 A260 corrected Formula for ssDNA FOI 8 77 Amax Eaye 10exp 6 A260 corrected Formula for RNA FOl 8 11 Amax Eaye 10exp 6 A260 corrected Formula for Oligonucleotides FOl 9 83 Amax Eaye 10exp 6 A260 corrected Amax AU Edye upmol cm A260 corrected A260 Cf2eo Amax Version 1 01 Page 58 ApDpendix Correction factors Absorption max Ext Coeff 260 nm Dye Type Dyes nm Nucleic Acid Alexa Fluor647 650 239000 09000 AlexaFluor660 660 107000 000 Alexa Fluor680 680 164000 000 L
78. 10 2 Parameter Screen Parameter Screen HN re Parameters Step 1 LabelGuard 1 Cuvette 2 Step 2 1 Nucleic Acids Step 3 5 6 7 8 Step 4 4 1 1 4 1 2 Pathlength Dilution SS Facfor Units Factor LabelGuard TY TI 0 3 2 Step 5 260 nm pye Correction Step6 Dye Type 10 Alexa 4 Cye 6 Oyster Texas Red 10 Appendix Results Screen Results Screen dsDNA Dye Step 7 Bla
79. New name Folder Step 3 OK Utilities Cancel Utilities Version 1 01 Page 53 Utilities 7 7 Serial Numher 15777215 OK Utilities WEersion TE wy_iImplen de EF 7 8 amesS ectro Elocks 7 6 1 Spectroblocks Escape Utilities Version 1 01 Page 54 Utilities 7 8 2 Sudoku Computer mode 50 User mode C
80. Results screen Cancel Protein Results Screen Step 11 Blank Step 12 A260 A280 A260 A230 Step 13 Step 14 Options 8 Step 15 Protein Options 1 parameters screen 2 3 on off 230 260 280 320 nm 220 nm 750
81. 0 001 to 9999 C OK Results screen Back Standards screen Results screen Step 23 Blank Step 24 Step 25 Step 26 Options Step 27 Protein Options 1 2 3 4 7 parameters screen on off 230 260 280 320 nm
82. EE 13 4 1 3 dsDNA ssDNA RNA 15 TE 17 AN i 18 2 CA 19 23 ERR 22 2 25 ca 28 4 3 0B6000 KK 31 RTA EE I Rl NN Wn i 33 5 RR ADIE UT RR RAN 34 36 5 38 AG 41 5 43 0 46 48 Es 50 EN 51 52 2 52 RC PR 52 53 7 53 ON 53 TA CO Naa es 54 TO 54 TBSpecCtEobIo CkS i i NR 54 LOZ SOOKU 55 56 8 56 56 8 7 56 57 58 TOETi 0 58 10 2 4 58 Version 1 01 Page 3 Table of contents 1 1 1
83. new standards Calibration standards ep cg7es OFF 1 2 3 Next Standards screen Cancel Protein Standards Screen i step 11 Standards Screen Step 12 Standards Step 13 Std 2 Std 5 D 4DD 1 nnn Std 3 Std 5 D 6DD 1 400 Version 1 01 Page 19 0 001 9999 C Next Calibration screen
84. 5 mm 10 mm LabelGuard 3 2 Lid Factor Step5 Dilution Factor 1 00 9999 C Options 0 019999 Parameter Screen Cancel Parameter Screen Step6 320 nm gac grog Step7 A260 Factor 0 76 1 00 9999 Step8 A280 Factor 1 55 1 00 9999 Step9 Units mg ml hg ml ng ul g l Step 10 OK
85. Step 2 6 Multi Wavelength Step 3 Wavelength Step 4 Pathlength LabelGuard applications 0 2 1 2 mm cuvette applications 5 mm 2 mm Step 5 OK Step6 first Wavelength Step 7 seco7g Wavelength Step 8 OK results screen Cancel Functions Results Screen Step 9 Blank Step 10 Step 11 KNhb
86. Theme TITRE EZ NI ET ON Spectro Blocks Sudoku Version 1 01 Page 51 Utilities 7 1 Date and Time Step 1 Day Step 2 Month Step 3 Year Step 4 Hou Step 5 Minute OK Step 6 OK Utilities Cancel Utilities 7 2 Luce Step 1 Number Format Se G 6 Humber Format _ TFT Step 2 OK Utilities Cancel Utilities 2 wm 73 Step 1 Auto pri
87. Standards screen Cancel Funcons Version 1 01 Page 43 Functions Standard Screen tandard EUrwe Standards td 1 St 4 IH Std 30 D Standard Curve Calibration Standards LF a 0 15 D Dd5 HUBS In 11 L147 8 1r 0 05 1 gh 30 HH 50 60 2 i el al a a Salealelse Calibration Screen replicates on Standard Curve Calibration Replicates 1 a D DS32 0 15 Calibration Manual entry Version 1 01 Standards screen Step 11 0 001 9999 Step 12 Next Calibration screen Back Parameter screen Calibration Screen replicates off Step 13
88. on off RE 4 7 ample Humber RMO 8 User Methods 1 9 9 Auto print auto print on off to Frint options Version 1 01 Page 45 FunctionSs 5 6 5 Parameter Screen Multi Ww avelength Farameters IE 1 lt Fathlength Results Screen Multi Fauvelength a 0 3o 1400 Version 1 01 HH nm nm SO nm EO nm ui ww avelength bs mnlE 300 nm OD nm FO nm EO nm D D1S D D1E D D13 D D13 Parameter Screen Step 1 3 Functions
89. 4Dso 2a7ce 9 Transmission Mode TR Step 10 Pathlength LabelGuard applications 0 2 1 2 mm cuvette applications 5 mm nk cance 2 mm Step 11 OK results screen CancelO Functions Result Screen Single Ww avelength Result Screen sampe Step 7 Blank a 150 Step 8 Step 9 Single Ww avelength Step 10 Step 11 15nm Step 10 Options
90. NanoPhotometer 1 9469 01 PATI KA Implen GmbH Wehrlestrasse 33 D 81679 Germany NanoPhotometerTM EC Implen NanoPhotometerTMW EC 73 23 EBC amp 89 336 EEC EN 61010 1 2001 EN 61326 2 3 1998 EN 61000 4 6 1992 1 2006 6 1 Ya Sr Dr Thomas Sahiri Managing Director Implen GmbH Version 1 01 Page 2 Table of contents 10 2025 Bt EK 65 5 4 5 5 22 5 5 6 TM LABELGUARD 8 3 8 92 9 LABELGUARD APPLICATIONS CUVETTE APPLICATIONS es 10 4 1 DNA RNA es 10 4 1 1 dsDNA ssDNA RNA ee 12
91. 5 t dA on off to gt 2 5A or lt 0 3A 7 8 User Methods 1 9 9 Auto print auto print on off 6 options Page 42 Functions 5 5 9
92. Step 11 Escape Functions Version 1 01 Page 34 FunctionSs Options Farameters Print bs Print Graph nn umber Lh ta Frint Version 1 01 Options 1 parameters screen 2 3 Absorbance T 4 7 8 User Methods 1 9 9 Auto print auto print on off options Page 35 Functions 5 2
93. auto print on off options Peak Detection 4 Au7o Detect Peaks on off Minimum Peak Height 2 Minimum Peak Width 2 Peak Detect on Zoom off Sort Peaks by Draw Peaks on off
94. methods Delete Method 1 delete method User Methods Lock Method 2 lock method Oser Methods Unlock Method 3 lock unlock method Ab User Methods Page 50 User 7 Utilities 1f2 1 Cate and Time oe Hames I ri 4 sso el ames TA
95. 1 9 Pathlength 5 mm 10 mm LabelGuard 3 2 Lia Factor 2 ul Units 8 Options hg ml ug pl pmol pl mg dl_mmol hmol g l mg ug UI ppm ppb conc OK 0 2 5 1 98768 2 98768 OK CancelW Next standards manual
96. Hz 2 3 Version 1 01 Page 4 Essential safety notes 2 2 1 CCD 1024 UV
97. Oyster 656 660 200000 004 Average Detectioh Rande and Required Sample Volumes for Undiluted Nuecleic Acid Samples In the NanoPhotometer 1 mm Lid Factor 10 0 mm Lid Factor 50 Total Detection actor Ingipl ng Range ngipl dsDNA 15 HO00 100 4 000 15 4 000 FN A 37 12 DD BU 3 950 12 3 950 12 64U B5 3 200 12 3 200 Oligo 3 10 480 DD 10 400 Corresponcding Absorbance Value 0D 025 to 1 6 Abs 025 fg 1 6 Abs Limits for A 260 Required ample Volume 3 0 7 to 4H 550 8527 2 1 27 TEL 06 6447 8633 FAX 06 6447 8683 103 0007 2 6 4 TEL 03 3249 2072 FAX 03 3249 2100 Mail bio so as 1 co jp URL http vww bio lab jp Version 1 01 Page 59 ApDpendix
98. S Step 16 Results screen 0 Back Standard andards Screen o 5 c Calibration Screen replicates On Step 17 Calibration Screen replicates On Bradrord Calibration Blank Step 18 replicate C Step 19 ES Step 20 replicate
99. een Factor Step 10 Blank Step 11 Page 36 Functions Result Screen standard Concentraktion Run Standard Concentration pm raw lernn rh ZED mm oncentration Absorbance D Ddz 150 ar ml 3571 Options Farameters Frint r Hun 5 r Frnnl umber uh tao Frint Version 1 01 Result Screen standard Step 12 Blank Step 13 Run Standard screen Step 14 gt standard Cancel measure screen Step 15 Step 16
100. l new standards Calibration standards PRepjicates OFF 1 2 3 Step 10 Next Standards screen Cancel Protein Standards Screen Step 11 0 001 9999 C Step 12 Next Calibration screen Page 25
101. nk Asao oma Step8 Ao nzeaa ME A260 A280 Ae50 m148A FOI CCy3 A260 A230 TRI i ES Step 9 1 73 nmall Step 10 Options Step 11 Nucleic Acids Version 1 01 Page 15 LabelGuard cuvette applications Options Farameters Print raph Frint ata nl 3mplE hrmber h nH Tint Version 1 01 Options 1 parameters screen 2 3 on off 230 260 280
102. nm 4 on off 7 8 User Methods 1 9 9 Auto print auto print on off options Page 18 LabelGuard cuvette applications 4 2 2 BCA Parameter Screen EC A Farameters Step 1 Pathleneth step 2 Fan step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Farameters Step 10 Calibration tandards 1 1 Parameter Screen LabelGuard 1 Cuvette 2 2 Protein 2 BCA Wavelength 562 mm Standard
103. nt on off Auto print on off Options OFF Step 2 Pu777 in US Bluetooth ok Step 3 OK Utilities Cancel Utilities ut Frimt n Version 1 01 Page 52 Utilities 7 4 take Step 1 Games games yes no Step 2 Screen layout Theme grid list mtn Standby Theme Step 3
104. p 5 Duration RE Step 6 terval 5 10 20 30 60 Step7 Next parameters screen Cancel Functions Kinetics Parameters 2 Step 8 Mode Delta A DMSO Final A Slope Step9 Units 8 Options ug ml ug ul pmol ul mg d mmol mol l g l mg ug U ppm ppb conc OK 0 2
105. r Screen Parameter Screen Step 1 1 LabelGuard Applications Farameters Step 2 1 Auc e c Acids 2 du Mo Protein Step 3 Step 4 Lia Factor 0 7 4 HI 3 5H Background Step 5 LabelGuard Applications Cuvette Applications Version 1 01 Page 9 LabelGuard Microliter Cell 4 LABELGUARD APPLICATIONS CUVETTE APPLICATIONS NanoPhotometer CabeiGuard 0 7 ul 5 ul 10 mm Utilities
106. s Results Screen Blank A2607A280 Options Step 14 Nucleic Acids Page 13 LabelGuard cuvette applications Options Farameters Frint Graph Frint Data nl 3mplE hrmber h nH to Frint Version 1 01 Options 1 parameters screen 2 3 on off 230 260 280 320 nm
107. tandard 1 9 Pathlength 5 mm 10 mm LabelGuard 3 2 Lid Factor 2 Ll Units 8 Options hg ml ug pl pmolul mg dI mmoMl umoM g l mg ug U ppm ppb conc OK DP 0 2 5 1 98768 2 98768 OK Cancel Next standards manua
108. ter selection OK On off on off 4 1 C 1 EeeUBaek SCdDG ance dCK 0 000 A Blank Reference 100 T
109. uto detect Feaks Feak etect on mnmm Hin Fk Height Sork Feaks Hr TT waseren in Fk width Hrawmr Fa sm we oe Aaan m J 1 CY a 4 420 dn 460 460 EOD EE EE ns aaaseaaa Sample 1 5Hnm bs 1 Version 1 01 Options 1 parameters screen 2 3 Absorbance T 4 Peak Detection Parameter Screen 5 results screen User Defined Peak Delete Peak 6 Graph Scale Parameter Screen 7 8 User Methods 1 9 9 Auto print
Download Pdf Manuals
Related Search