トランケートされたr47タンパク質およびトランケートされたr56タンパク質
Contents
1. 50 10000 HHHHHHHHHH 000000000000 00000 JP 2005 538948 2005 12 22 7 10 I uuuuuuuunuuunonw HHHHHHHHHHHHHH 30 2G2. SEO ID No 1 Truncated Protein Sequence of r47 Protein Expressed truncated protein seguence translated from 184 1458 of DNA seguence bolded seguence was confirmed by N terminal protein seguencing MVNSLSDIVEPLISTVVSIYAVDTNIGISFNNKVSKYOOEVFLGSGVIIDSSGYIVTNENVIA GAENIKVKLHDGSELIAELVGSDNKINIALLKINSPAALSYATFGDSNOSRVGDOVIAIGSPF GLRGTVTNGIISSKGPDMGNGIVTDFIQTNA AIHMGSFGGPMFNLEGKIIGINSIHVSYSGISF AIPSNTVLEAVECLKKGEKIRRGMLNVMLNELTPELNENLGLKODONGVLITEVIKEGSAA QCGIAPGDVITKFHDKEIKTGRDLQV AVSSTMLNSEREVELLRNGKSMTLKCKIIANKGEDS EQQSNDQSLV VNGVKFVDLTPDLVKKYNITSANNNGLFVLEVSPNSSWGRYGLKMGLRPR DIILSVKRDDNKKDISVKTLREIVTNIKHNEIFFTV ORGDRMLYIALPNINK Protein sequence data output only N terminal MVNSLSDIVEPLISTVV 10000 SEQ ID No 2 r56 Protein Karp Ching The confirmed sequence for r56 by Ching amino acid 80 456 The corrected sequence is highlighted and underlined MTIAPGFRAEIGVMYLTNITAQVEEGKVKADSVGETKADSVGGK DAPIRKRFKLTPPOPTIMPISIAVRDFGIDIPNIPOOOAOAAOPOLNDE ORAAARIAWLKNCAGIDYRVKNPNDPNGPMVINPILLNIPOGNP NPVGNPPORANPPAGFAIHNHEOWRHLVVGLAALSNANKPSASP VKVLSDKITQIYSDIKHLADIAGIDVPDTSLPNSASVEQIQNKMQ ELNDLLEELRESFDGYLGGNAFANOIOLNFVMPOOAOOOGOGO OOOAOATAOEAVAAAAVRLLNGNDOIAOLYKDLVKLORHAGIE KAMEKLAAQQEEDAKNQGEGDCKQQQGTSEKSKKGKDKEAEFD LSMIVGQVKLYADVMITESVSI PI 5 8 MW 40903 377 a a Extinctio
3. 50 2005 538948 2005 12 22 10 20 30 50 JP 2005 538948 2005 12 22 9 10 20
4. 47 r 56 57 0 000 000000000000000000000000000000000000000 000000000000000000000000000000000000000 00000000000000000000 0 2 JP 2005 538948 2005 12 22 10 00000 BE ls SEP RT Aa EE EE ER EI EI EE I UUUGUdUU
5. HHHHHHHHHHHHHHHH HHHHHHHHHHHHHH 50 10000 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH JP 2005 538948 2005 12 22 4 10 20 I uuuuuuuunuuunonw HHHHHHHHHHHHHH 1000 IU
6. HHHHH 0000000 30 000000 000000 50 HHHHHHHHHHHHHHHH JP 2005 538948 2005 12 22 5 10 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 20 30
7. HHHHH 10000000 HHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 50 JP 2005 538948 A 2005 12 22 6 10 20 nnnnnnnnnnnnnnnm 30
8. HHHHH OUUU HHHHHHHHHHHIH OUUU HHHHHHHHHHHIH HHHHH HHHHHHHHHHH HHHHH LU HHHHHHH HHHHHHHHHHHHH OUUU HHHHHHHHHHH 00000 HHHHHHHHHHHIH 10 20 30 50 JP 2005 538948 2005 12 22 3 10 DICE EE RICE E EP CEDE BELLE HI HHHHHHH 10000000 000000000000 000000000000 20 ID 30 ID UU
9. Crete EEE 6EZEOTdN au 622723 gt 070070 YA GE 15196 DIV 082 oup D LE ost 85 XH OO e CD Bl CO 2 9 o1n r ELE Lr S 19 JP 2005 538948 A 2005 12 22 HHHHHHHH INTERNATIONAL SEARCH REPORT International application PCT US03 15072 CLASSIFICATION OF SUBJECT MATTER IPC 7 A61X 39 00 39 02 39 40 CO7K 16 12 USCL 424 184 1 234 1 530 388 2 388 4 According to International Patent Classification IPC or to both nationa classification and IPC B FIELDS SEARCHED Minimum documentation searched classification system followed by classification symbols U S 424 184 1 234 1 530 300 350 530 388 2 388 4 435 4 7 1 7 2 7 32 7 22 7 9 7 92 7 93 Documentation searched other thau minimum documentation to the extent that such documents are included in the fields searched Electronic data base consulted during the international search name of data base and where practicable search terms used WEST PubMed Medline CAPLUS PASCAL BIOSIS EMBASE SCISEARCH Citation of document with indication where appropriate of the relevant passages Y CHING et al Identification of Human Antibody Epitopes on the 47 KDa 56 KDa and 22 1 6 7 12 13 kDa Protein A
10. 30 50 1000 UN 00000 DH D 0 10 JP 2005 538948 2005 12 22
11. 07 23 0652 EEO1 GM 5 A90 DA50 1 EA52 FA 4
12. 1021 tcttcaacta tgettaatte tgaaagagaa gttgagcttt tacgtaatgg taagtcgatg 1081 actctaaaat gtaaaattat tgccaacaaa ggtgaggata gigagcaaca aagtaatgat 1141 caaagccttg tggttaatgg tgtaaaattt gttgatctta cacctgattt agtgaagaaa 1201 tataatatta cttcagctaa taataatggg ttatttgtcc ttgaagtttc gcctaactct 20 1261 tctteeggga gatatgottt aaaaatgggg ctaagaccta gagatataat tttatcagtt 1321 aaacgtgatg ataataaaaa agatatttct gttaaaactc taagagaaat agtgacaaat 1381 ataaagcata atgaaatttt ctttacagtg caaagaggag atagaatgct ttacattgct 1441 ttacctaata ttaataag ta aatctagttt aaaggcgatt t 10 JP 2005 538948 2005 12 22 17 o7 WN Aen Toc ok WN VIN HET N EE ar n N Nu 1 DIAI ELE 6r v9 AW A gl CO Lt OGC ER IEEE BE PSP LVL SOLO YE PS 24 46 ARL Uc HHA AA VNQdL 1 2WNO9ML 1 da BEP SEE I 94 IL 10 59 955 LE 1 17 PIS AW HELOBELLIOT O HO y o1n314 BE LE 9t SE ve ce LE 6r 9 ae O v Ecc E AAvaaA JP 2005 538948 A 2005 12 22 18 ona g cnc YODA mM n Uw YODA ci TrZz63bd VAH
13. 19 JP 51 Int C1 CO7K A61K A61K A61K A61P 14 29 39 00 39 02 39 395 31 04 33 2004 504973 P2004 504973 15 5 15 2003 5 15 17 1 11 2005 1 11 PCT US2003 015072 W02003 096974 15 11 27 2003 11 27 60 380 301 14 5 15 2002 5 15 US DRA Re RA RA 14 29 39 00 39 02 39 395 31 04 74 74 72 JP 2005 538948 2005 12 22 11 2005 538948 P2005 538948A 43 R17 12A22E 2005 12 22 2 ZNA 4BO24 2 4C085 4HO45 N BERK RK TREER A 23 B 71 504238563 AF Ww 2221 7 800 100083806 100095500 FR 208 17 NEAR 8014 54 H 1 V
14. catctaatac tgtacttgaa 781 gcagttgaat gcttaaaaaa aggagaaaaa attcgtcgtg gtatgttaaa tgttatgctt 841 aatgaattaa ctccagaatt aaatgagaat ttaggactta aacaagatca aaatggagtt 901 ctaataactg aagttataaa agaaggatct gcagcacaat gtggaattgc tcctggagat 20 961 gtaattacta aatttcatga taaagagatc aaaacaggga gagatttaca gotagctgta 1021 tcttcaacta tgcttaattc tgaaagagaa gttgagcttt tacgtaatgg taagtcgatg 1081 actctaaaat gtaaaattat tgccaacaaa ggtgaggata gtgagcaaca aagtaatgat 1141 caaagccttg tggttaatgg tgtaaaattt gtteatctta cacctgattt agtgaagaaa 1201 tataatatta cttcagctaa taataatggg ttatttgtcc ttgaagtttc gcctaactct 1261 tcttggggga gatatggttt aaaaatgggg ctaagaccta gagatataat tttatcagtt 1321 aaacgtgatg ataataaaaa agatatttct gttaaaactc taagagaaat agtgacaaat 1381 ataaagcata atgaaatttt ctttacagtg caaagaggag atagaatgct 1441 ttacctaata ttaataag ta aatctagttt aaaggcgatt t 30 10 50 10 15 20 25 10 15 20 14 JP 2005 538948 2005 12 22 SEOUENCE LISTING SEO ID No 1 Truncated Prot
15. KIDG STRLDPADAG PMPORYKLTP DPNNPOARIV OIYRDVKPFA AQQOGQGOQO NKKKRGASED VDIKANTGMV STRLDSTDSE GGGTDTPIRK VPDPONPNAR TKIYSDIKPF eA9000G0G9 GEGDCKQQQG HTGMVASGAL 10 20 30 12 JP 2005 538948 2005 12 22 Primer sets a Forward primers Gene 125 174 as target gene seguence 5 tatgcat aCCATGGtaa atagtttatc tgat 3 SEO ID No 5 site b Reverse Primers Gene seguence of 1443 1477 as target 35 bp 5 cgcctGGATCC tagatttacttattaatattaggt 3 site SEO ID No 6 10 20 13 JP 2005 538948 A 2005 12 22 SEO ID NO 7 amplified seguence 174 1481 nt Expressed DNA seguence 184 1458 nt aa 42 466 the coding region is bold 174 tatgcat 181 atg gtaa atagtttatc tgatatagtt gagccattaa tatctacagt agtaagtatt 241 tatgctgtag atactaacat tggtattagt tttaataata aggtatctaa gtatcagcaa 301 gaagtgttct taggttctgg ggttatcatt gatagttctg ggtatattgt tactaatgag 361 aatgttatag caggagctga aaatataaaa gtaaagttgc atgatggttc agaactcata 421 gcagaattag ttggtagtga caataaaatt aatatagctt tattaaaaat taattctcca 481 gcagcattat cttatgcgac ttttggcgac tcaaatcagt ctagagtagg agatcaggtt 541 attgcaatag gaagtccttt tggtttaaga ggaacagtaa caaatggcat tatttettet 601 aaaggaccag atatgggtaa cggcatagta actgatttta ttcaaactaa tgctgctatt 661 catatgggta gctttggtgg accgatgttt aatcttgaag gaaaaattat tggaattaat 721 tccattcacg tatcttactc aggcataagt tttgctatte
16. SD PLPNSASVEO QQAQATAQEA AAAAAVRVLN SDAGGASKGG ASGALGVAIN AAEGVYVDIE SEO ID No gt Gilliam MKKIMLIASA GKKHLSLTTG LPFGGTLAAG RFKLTPPOPT IMPISIADRD VINPVLLNIT OGPPNVOPRP ADIAGIDVPD TGLPNSASVE OOOAOATAOE AVAAAAVRLL ASEKSKEGKG RETEFDLSMI GVAINAAEGV YVDLEGSYMH Primer sets 15 3 r56 Protein Seguence Kato sequence MSALSLPFSA MTIAPGFRAE NVPOGGANHL OPCSILNHDH IQNKMQELND NNDOIIRLYR DLSMIVGQVK GSYMHSFSKI number 21 SAIELGDEGG LGVMYLANVK GDNLGANDIR WRHLVVGITA ILDEIRDSFD DLVRKLRRHAG LYADLFTTES EERYSINPLM 529 aa LECGPYAKVG AEVESGKTGS RADDRITWLK MSNANKPSVS GCIGGNAFAN IKKAMEELAA FSIYAGLGAG ASFGVRYNF 4 156 Protein Gilliam sequence MSALSLPFSA MTIAPGFRAE VGVDTDILAQ RONLDILDHG OIOSKMOELN NGNDOIAOLY VGQVKLYADL SFSKIEEKYS a Forward primers number 25 SAIELGEEGG LGVMYLRNIS AAAGOPOLTV QWRHLVVGVT DVLEDLRDSF KDLVKLQRHA FTTESFSIYA INPLMASVGV Gene 125 174 as target gene sequence 524 aa LECGPYGKVG AEVEVGRGEV EORAADRIAW ALSHANKPSV DGYMGNAFAN JP 2005 538948 A 2005 12 22 VVGGMITGVE DADIRSGADS NYAGVDYMVP PIKVLSEKIV OIOLNFRIPO GDGGCNGGGD LAYTSGKIDG IVGGMITGAE DSKGEIKADS LKNYAGIDYM TPVRVLSDEI OIOLNFVMPO GVKKAMEKLA AOOEEDAKNO GVGAGLAHTY RYNF GKIDDKDIKG 5 tatgcat aCCATGGtaa atagtttatc tgat 3 SEO ID No 5 Neo I site b Reverse Primers Gene sequence of 1443 1477 as
17. and third sentences of Rule 6 42 H Observations where unity of invention is lacking Continuation of Item 2 of first sheet This International Searching Authority found multiple inventions in this international application as follows As all required additional search fees were timely paid by the applicant this international search report covers all searchable claims 2 C As all searchable claims could be searched withoitt effort Justifying an additional fee this Authority did not invite payment of any additional fee As only some of the required additional search fees were timely paid by the applicant this international search report covers only those claims for which fees were paid specifically claims Nos No required additional search fees were timely paid by the applicant Consequently this international search report is restricted to the invention first mentioned the claims it is covered by claims Nos Remark on Protest The additional search fees were accompanied by applicant s protest No protest accompanied the payment of additional search fees Form PCT ISA 210 continuation of first sheet 1 July 1998 22 JP 2005 538948 A 2005 12 22 PCT US03 15072 INTERNATIONAL SEARCH REPORT Continuation of Box Reason 2 These claims read on inventions relating to specific protein sequences However no computer readable form of the indicated sequences was provides such th
18. at no meaningful search could be made of the claimed inventions of these claims Form PCT ISA 210 second sheet July 1998 5 0000 Il 81 31 18 43 00 33 53 15 09 23 JP 2005 538948 2005 12 22 IHHHHHHHHH IHHH 3y18 0000 430 HHH IHHH 3753 D 1000 1500 D AP CH GM LS SD SL SZ TZ UG ZM ZW EA AM AZ BY KG KZ MD RJ TJ TM EP AT BE BG CH CY CZ LE EE ES FI FR HJ IE IT LU MG PT RO SE SI SK TR OX EF CF GO MG GY GQ GWM M NE SN TD TG AE AG AL AM AT AU AZ BB BG BR BY BZ CH CN QJ CE K EZ EC EE ES Fl G Q H GM FR HJIDILINIS JP KE KG KP KR KZ LG LK LR LS LT LU LV M M M M WN MZ NO NZ OM PH PL PT RO RU SD SE SG SK SL TJ TM TN TR TT TZ UA UG VN YU ZA MAN 72 72 E Ll 100 00 n n n n EJ UU 48024 AAO AAI5 BA35 CA CA5 GO 40085 BAIS BA47
19. ein Seguence of r47 Protein Expressed truncated protein seguence translated from 184 1458 of DNA seguence bolded sequence was confirmed by N terminal protein seguencing MVNSLSDIVEPLISTVVSIY AVDTNIGISFNNEVSKYOOEVFLGSGVIIDSSGYIVTNENVIA GAENIKVKLHDGSELIAELVGSDNKINIALLKINSPAALSYATFGDSNOSRVGDOVIAIGSPF GLRGTVTNGIISSKGPDMGNGIVTDFIQTNAAIHMGSFGGPMFNLEGKIIGINS 1H VS YSGISF AIPSNTVLEAVECLKKGEKIRRGMLNVMLNELTPELNENLGLKODONGVLITEVIKEGS AA OCGIAPGDVITKFHDKEIKTGRDLOVAVSSTMLNSEREVELLRNGKSMTLKCEUANKGEDS EQQSNDQSLVVNGVKFVDLTPDLVKKYNITSANNNGLFVLEVSPNSSWGRYGLKMGLRPR DIILSVKRDDNKKDISVKTLREIVTNIKHNEIFFTV ORGDRMLYIALPNINK Protein sequence data output only N terminal MVNSLSDIVEPLISTVV SEQ ID No 2 r56 Protein Karp Ching The confirmed sequence for r56 by Ching amino acid 80 456 The corrected sequence is highlighted and underlined MTIAPGFRAEIGVMYLTNITAQVEEGKVKADSVGETKADSVGGK DAPIRKRFKLTPPOPTIMPISIAVRDFGIDIPNIPOOOAOAAOPOLNDE QRAAARIAWLKNCAGIDYRVKNPNDPNGPMVINPILLNIPOGNP NPVGNPPORANPPAGFAIHNHEOWRHLVVGLAALSNANKPSASP VKVLSDKITQIYSDIKHLADIAGIDVPDTSLPNSASVEQIQNKMQ ELNDLLEELRESFDGYLGGNAFANQIQLNFVMPQQAQQQGQGQ OOOAOATAOEAVAAAAVRLLNGNDOIAOLYKDLVKLORHAGIK KAMEKLAAQQEEDAKNQGEGDCKQQQGTSEKSKKGKDKEAEFD LSMIVGOVKLYADVMITESVSI PI 5 8 MW 40903 377 a a Extinction coefficient A3g0 0 754 1 mg ml 10 20 30 10 15 20 10 15 20 SEO ID No gt Kato MKKIMLIASA GKKOLPLTTS MPFGGTLAAG POPTIMPISI ADRDLGVDIP NPVLLNIPOG PPNANPROAM RVAGIEVP
20. itation or other special reason as specified document referring to an oral disclosure use exhibition ar other means document published prior to the international filing date but later than the Name and mailing address of the ISA US See patent family armer later document published after the international filing date or priority date and not in conflict with the application but cited to tnderstand the principle or theory underlying the invention document of part on ar relevance the claimed invention cannot be considered novel or cannot be considered to involve an inventive step when the document Is taken alone document of particular relevance the claimed invention cannot be considered to involve an inventive step when the document is combined with or more other suck documents such combination being obvious to a person skilled n the art document member of the same patent family Date actual completion of the international search Date of PIN of the international search report 14 April 2004 14 04 2004 ied JU 004 f Mail Stop PCT Attn ISA US V Commissioner for Patents 1450 Alexandria Virginia 22313 1450 Facsimile No 703 305 3230 Form PCT ISA 210 second sheet July 1998 elephone No 571 272 1600 20 JP 2005 538948 A 2005 12 22 PCT US03 15072 INTERNATIONAL SEARCH REPORT C Coni nuation DOCUMENTS CONSIDERED TO BE RELEVANT Citation of document
21. n coefficient Azsp 0 754 1 mg ml 10 20 30 50 SEO ID No 3 gt Kato seguence MKKIMLIASA MSALSLPFSA GKKOLPLTTS MPFGGTLAAG MTIAPGFRAE PQPTIMPISI ADRDLGVDIP NVPQGGANHL NPVLLNIPQG PPNANPRQAM QPCSILNHDH RVAGIEVPSD PLPNSASVEQ IQNKMQELND QOAQATAQEA AAAAAVRVLN NNDOIIKLYK SDAGGASKGG KGKETKETEF DLSMIVGQVK ASGALGVAIN AAEGVYVDIE GSYMHSFSKI SEQ ID No gt Gilliam MKKIMLIASA GKKHLSLTTG LPFGGTLAAG RFKLTPPOPT IMPISIADRD VINPVLLNIT QGPPNVOPRP ADIAGIDVPD TGLPNSASVE QOOAQATAQE AVAAAAVRLL ASEKSKEGKG KETEFDLSMI GVAINAAEGV YVDLEGSYMH sequence MSALSLPFSA MTIAPGFRAE VGVDTDILAQ RONLDILDHG OIOSKMOELN NGNDOIAOLY VGOVKLYADL SFSKIEEKYS 11 number 21 SAIELGDEGG LGVMYLANVK GDNLGANDIR WRHLVVGITA ILDEIRDSFD DLVKLKRHAG LYADLFTTES EEKYSINPLM number 25 SAIELGEEGG LGVMYLRNIS AAAGOPOLTV QWRHLVVGVT DVLEDLRDSF KDLVKLORHA FTTESFSIYA INPLMASVGV r56 Protein Seguence Kato 529 aa LECGPYAKVG AEVESGKTGS RADDRITWLK MSNANKPSVS GCIGGNAFAN IKKAMEELAA FSIYAGLGAG ASFGVRYNF A r56 Protein Gilliam 524 aa LECGPYGKVG IVGGMITGAE AEVEVGKGKV DSKGEIKADS EORAADRIAW LENYAGIDYM ALSHANKPSV TPVKVLSDKI DGYMGNAFAN OIOLNFVMPO GVKKAMEKLA AOOEEDAKNO GVGAGLAHTY GKIDDKDIKG RYNF JP 2005 538948 2005 12 22 VVGGMITGVE DADIRSGADS NYAGVDYMVP PIKVLSEKIV OIOLNFRIPO QDGGCNGGGD LAYTSG
22. ntigens of Orientia tsutsugamushi with Synthetic Peptides American Journal of Tropical Medicine and Hygiene 1996 Volume 55 page 300 abstract 608 CHING et af Expression and Refolding of Truncated Recombinant Major Outer Membrane Protein ANtigen 56 of Orientia tsutsugamushi and Use in Enzyme Liked immunosorbent Assays Clinical and Diagnostic Laboratory Immunology July 1998 Vol 22 5 No 4 pages 519 26 HICKMAN et al Murine T cell Response to Native and Recombinant Protein Antigens of Rickettsia tsutogamushi Infection and Immunity May 1993 Vol 61 No 5 pages CHING et al Early Diagnosis of Scrub Typhus with a Rapid Flow Assay Using Recombinant Major Outer Membrane Protein Antigen r56 of orientia tsutugammshi Clinical and Laboratory Immunology Mar 2001 Vol 8 No 2 pages 409 14 1 6 7 12 13 18 1 6 7 12 13 18 SEONG et al Neutralization Epitopes on the Antigenic Domain H of the Orientia tsutugamuchi 56 kDa Protein Revealed by Monolconal Antibodies Vaccine 2001 Vol 19 pages 2 9 1 6 7 12 13 18 Further documents are listed in the cont nnation of Box L Special categories of cited documents document defining the general state of the art which s not cnns dered to be of particular r ievance earlier application or patent published on or after the international filing date document which may throw donhts on priority claim s or which is cited to establish the publication date of another c
23. target 35 bp 5 cgcct GGATCC tagatttacttattaatattaggt 3 BamHl site SEO ID No 6 STRLDPADAG PMPORYKLTP DPNNPQARIV QIYRDVKPFA NKKKRGASED VDIKANTGMV STRLDSTDSE GGGTDTPIRK VPDPONPNAR TKIYSDIKPF QAQQ00GQGQ GEGDCKQOQG HTGMVASGAL 10 20 30 16 JP 2005 538948 2005 12 22 SEO ID 7 amplified seguence 174 1481 nt Expressed DNA seguence 184 1458 nt aa 42 466 the coding region is bold 174 tatgcat 181 att atg gtaa atagtttatc tgatatagtt gagccattaa tatctacagt agtaagfatt 241 tatgctgtag atactaacat tggtattagt tttaataata aggtatctaa gtatcagcaa 301 gaagtgttct taggttctge ggttatcatt gatagttctg ggtatattgt tactaatgag 361 aatgttatag caggagctga aaatataaaa gtaaagttgc atgatggttc agaactcata 421 gcagaattag ttegtagtga caataaaatt aatatagctt tattaaaaat taattctcca 481 gcagcattat cttatgcgac ttttggegac tcaaatcagt ctagagtagg agatcaggtt 541 attgcaatag gaagtccttt tggtttaaga ggaacagtaa caaatggcat tatttettet 601 aaaggaccag atatgggtaa cggcatagta actgatttta ttcaaactaa tgctgctatt 661 catatgegta gctttggtge accgatgttt aatcttgaag gaaaaattat tggaattaat 721 tccattcacg tatcttactc aggcataagt tttgctatte catctaatac tgtacttgaa 781 gcagttgaat gcttaaaaaa aggagaaaaa attcgtcgtg gtatgttaaa tettatectt 841 aatgaattaa ctccagaatt aaatgagaat ttaggactta aacaagatca aaatggagtt 901 ctaataactg aagttataaa agaaggatct gcagcacaat gtggaattgc tcctggagat 961 gtaattacta aatttcatga taaagagatc aaaacaggga gagatttaca ggtagctgta
24. with indication where appropriate of the cefevant passages Relevant to claim No OAKS et al Antigenic and Genetic Relatedness of Eight Rickettsia tsutugamushi 1 7 12 13 18 Antignes Infection and Immunity October 1989 Vol 57 No 10 pages 3116 22 WATT et al HIV 1 Suppression During Acute Scrnb Typhus Infection The Lancet August 2000 Volume 356 pages 475 79 WATT et al Passive Transfer of Scrub Typhus Plasma to Patients with AIDS a Descriptive Clinical Study Ouarterly Journal of Medicine 2001 Vol 94 pages 599 607 Form PCT SA 210 second sheet July 1998 21 JP 2005 538948 A 2005 12 22 RNATIONAL SEARCH REPORT n national application No PCT US03 15072 Box I Observations where certain claims were found unsearchable Continuation of Item 1 of first sheet This international report has not been established in respect of certain claims under Article 17 2 a for the following reasons 1 Claim because they relate to subject matter not required to be searched by this Authority namely Claim Nos 2 5 8 11 14 17 19 21 and 23 25 because they relate to parts of the international application that do not comply with the prescribed requirements to such an extent that no meaningful international search can be carried out specifically Please See Continuation Sheet Claim Nos because they are dependent claims and are not drafted in accordance with the second
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