平成16年12月2日
Contents
1. 16 3905863 2 2007 4 18 3 DNA Genbank 004698 2 Pseudomonas aeruginosa 87 862. 10 HHHHHHHHHHHH HHHHHHHHHHIH 20 30 40 HHHHHHHHHHHH 50 2 2007 4 18 10 20 30
3. n 20 50 14 3905863 2 2007 4 18 Torsvik V et al Prokaryoti c di versi ty magni tude dynam cs and controlling fa ctors Sci ence 2002 296 1064 1066
4. 72 3 7 5 1 4 57 7HHHHHHHHH IHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 10 IHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHH IHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHH HHHHHH IHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHH HHHHHH 20 3905863 2 2007 4 18 2 n n 10 1
5. N m in CN E ma 3 10 D d r3 C wN a EIE DO 3 ux D DL 0 o g Do 3 a _ ON o g 3 C3 D N ma 3 DO E3 _ r3 lt o g m C3 rN EE oi EX EE SU C iu D ma o r3 sign eh TA ig en _ DI E E Ex 3 o0 3 P a ma m Sena 3 L3 zu D 095 C3 v 3 c N Do da 3 pipi 1 oaan D ES DO DI mas a TEN __ DI ENS TE C3 D ar ai D _ C DI 5 bud DI DI 5 o a LT gt 5 r7 DI DI o g g Ci s G E EJ mm cogo G _ E C3 C3 2c
6. n 1 10 20 30
7. HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHH ut htt p www bc cytometry conf add page fcnpri n htm D FACSCali bur Becton Di nson F ACSVant age SE Becton Di ckinson 00000 HHHHHHHHHHHHHHHHHHHHHHHHH 000 Sanbrook Fitsch EF Maniatis T Molecular Cloning A Laboratory Manua Cold Spring Harbor Cold Spring Harbor Press 198 0000000 52 CC et al Growth phase dependent transcription of the si gna 54 dependent Po pronoter controlling the Pseudononas deri ved net hy phenol operon of 150 J Bacteriol 1996 178 3727 350 000000000
8. 1000 LI I LI I 1 20 1 n 30 Phyl ogeneti c i dent et al RI m cr obi al Amman 40 cells wthout culti vatio 50 000000 59 143 69 n
9. 17 3905863 2 2007 4 18 HHHHHHHHHHHHHHH 1961 procedure for the i sol ati on of deoxyri bonucl ei c acid from sms J Mol Biol 3 208 218 U EL DT DP ER FE PD P PDP PD DD EJ 1
10. 000 000 000 phenol hydroxylase 1000000600 Nordl und I et al nuc eoti sequence and pol ypepti de analysis of multi component phenol hydroxyl ase f rom Pseudononas sp strain CF600 J Bacteriol 1990 172 6826 6833 Boss n B 1000000 1000000 Kasai Y et al The TOL plasmid N gene pro duct fromPseudononas putida is involved mxylene uptake J Bacteriol 2001 1 83 6662 6H HHHHHHHHHHHHHHHHHHHHHHHHHHH Duque E et al G obal cognate regulators control the expression of the organic sol vent efflux punps TtgABC and TtgDEF of Pseudomonas putida Ml Mcrobiol 2001 39 1100 6 00000000
11. 3905863 2 2007 4 18 6 10 A et Vel asco anal ysis of the styrene catabolic cluster of Psudom Bacteriol Genetic and functi onal tog al onas sp 1998 180 1063 1071 strain 2 J 20 n n n UU Environ M crobiol 20 MR et al
12. 9 N m in DI 3 r3 1 1 DI e 1 m C3 CN 3 3 3 C3 in __ m ei 1 DI 3 3 3 3 3 C3 3 3 m 3 T DI BE 5 N an C3 N DI N r3 m sa zp C3 im 3 r3 ci C3 222 o C3 a DI s a 3 3 3 um o EJ DI DI lt 42 gt DI m 3 C3 3 3 c 3 C3 gt Do c3 Bi iui se B 5 ea s r3 5 Q i DD r3 22 U lt 3 DI C3 G m m DI 4 I QI o DI lt N c OI DI 5 4 2 2 DI 3905863 2 2007 4 18
13. L1 stoni a eutropha HHHHHHHHHHHHHHHHHHHHHHH 0000060 0000010000 5 Phenol hydroxyl ase cloned from stoni eutropha strain E2 exhibits novel neti c properti es M crobi ol ogy 1998 144 1765 72 Ral st oni eutr opha hh B tH Ton Both p ha B 0000 Si 1 havy D Silhavy TJ et al Experi rent s wth gene fusi ons Cold Spring Harbor Laboratory Cold Spring Harbor N Y 19 84 10010 HHHHHHHHHHHHHHHHHHHHHQAquick gel extrac
14. AF31182051 3 Comamonas testosteroni 59 PPU24215 2 Pseudomonas putida 40 10 KPNBUDOPRN 1 Raouitella terrigena 49 VE HHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHH 20 DUDUH 110 120 130 160 170 210 211 212 213 220 223 400 19 SEQUENCE LISTING Marine Biotechnology Institute Method for isolating a metabolic system gene P03 0435 PatentIn version 3 1 1 3411 DNA Artificial vector 1 3905863 2 2007 4 18 gcgcccaata cgcaaaccgc gegtigecce attcattaat gcagctggca 60 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 120 cactcattag gcaccccagz ctttacactt tatgcttecg gCtCgtatgt tgtgtggaat 180 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct 240 10 20 30 20 cggtacccgg ggatcctaat taattaagaa ggagatatac gaacttttca ctggagttgt gttgaattag aaattttctg tcagtggaga gggtgaaggt gatgcaacat tttatttgca ctactggaaa actacctgtt ccatggccaa tatggigttc aatgcttttc aagataccca gatCatatga 3905863 B2 2007 4 18 atategciag caaaggagaa atggtgatgt taatgggcac a
15. UU 00000000000 1000 00000000000 HHHHHHHHHHHH 10 20 30 50 3905863 2 2007 4 18 9 10 30
16. Rev 1995 ificati on and in situ detecti on of i ndi vi dual M crobi ol n 3905863 2 2007 4 18 3
17. 8 8 2 n E i 10 9 19 10 b LB fil Acquisition Histogram Plot R 8 is 8 oi 109 es c M9 Z va Acquisition Histogram Plot Counts 229 15 20 25 309 5 enn Li RI 10 1 105 FLT H o ss 3905863 2 2007 4 18 0000 Acquisition Histogram Plot 15 20 25 s Counts m dn 100 10 n 5 B Acquisition Histogr m Plot 15 20 25 so Counts 10 26 RE 26 3905863 2 2007 4 18 5 HHHH http ww ncbi nl mn h gov ertrez ever f cgi 21674491 199113 Genes to Is 2000 5 p169 190 590000 un d p du 15 09 CDQ 168 GenBark EMBL GeneSeq B y VPI D ALOQ PubMed J STPI us J Dreand
18. 11 3905863 2 2007 4 18 Mnirmal Medi um Sambr ook J and Russell D W Molecular ird edition Vol une3 Cold Spring Harbor Laboratory Press 2001 2 2 2 4 oning Laboratory manuals n 000 n H no its S et al Phenol hydroxyl ase cl osed from Ral ston novel kinetic properties Mcrobi ol ogy 1998 144 a eutropha strain E2 exhi b 1765 1772 0000000 HHHHHHHHIH
19. 3 5 o o 3 gs uu DI DI Da 5 L3 DI DI ii L3 D O m mmo ES G gt DI DI DI D gt TaT E DI oam o Son gt E El m _ I S 8 3905863 2 2007 4 18
20. 3905863 2 2007 4 18 19 JP 0299 B2 11 3905863 3905863 45 19 4 18 2007 4 18 24 19 1 19 2007 1 19 61 Int CI F1 C12N 15 0 206 01 C12N 15 00 ZNAA C12Q 1 68 2006 01 C12Q 1 68 Z 10 26 E QU 2003 139080 P2003 139080 73 591001949 22 HERE 15 5 16 2003 5 16 65 2004 337101 P2004 337101A 3 7 5 1 43 16 12 2 2004 12 2 100091096 18 4 11 2006 4 11 74 100118773 mm B 1 4 30 72 100120905 3 75 1
21. gegcccaalaegeaaacegeticroccesegegtiggecgaticaftaatgcagctgrcacgucaggiileccgae ggaa agceggcagtgagegraacgcaatiaalgigauitageicacteattaggeaceccasgelltacactitatecitecege Legiatgttglgtenaat gtgagcggatnacnatttcacacaggaaacegetatgaccatgac incgual Legagetegg tacccggagatcciaattaattaagaaggagatatacala Lggetagca amp aggagaagaaetttteactggagtlgtceca altettgtigaattagatggtgatgt taatggecacaaattttetgtcagtezagagggt saaggtgotpcaace Lac gga aa aettacecttaaatttatitgcactactggaaedc bat e Lg LLecatggecaacacttgtcactactttetet Lat get glicaatgettiteaagatacccagalcatatganacggcatgactilitcaagagigocal gecegaagtt Lat Lacag gaaagaBcietaagcttcaaagatgacgggaactacaagecaecglgetgaagtcaagt ttpaaggLaataccottgttaat taaaaggtatigattttaaagaagatggacacattetLggacacaaatTggaatacaactataacieacae aatgtatacetcact amp cagacanacaaaagaatggaatcsaagctaactteaaasttagaescaacattgaagatzgaage Bl Lewat tageagac cat Latcaacaaaatactecaat tegegatggeectgtectittaccagacaaccattacctutoc acacgatetgeccitteganagateccaacgasazgagagaccacatggiceltettgaglitglaacageteciegeatt acacetggectgaalgaactatactaagcatgcaageLtggeactggecglegttttaceacategteact gegaaaaccc Lgsecgttacceaacttaatcgecttgcagcacatecccetttegecagetagegtaatagegaagagaccegcacegatcg cccitcecazcagttgcacagecigaatggegaatggcacetgatgoggtattttctecttacgcatctgtgcggtatite acaccgcatatgatgcactctcagtaenatetactetgatgecgcatagttaagccagcccegaeacccgecaacaccegc t amp acgcgccc LgacgggetLgtetgeteceggcatecgettacagacaage t gLgategtet ecgggagetgcatetgtca gaggtlttcaccgtcatcaccgaascgcBCEngac
22. Appl Rondon 10000 S B 50 66 2541 2547 00 3905863 2 2007 4 18 7
23. I D D D DE UO EE UE UE CE CE C 10 20 30 50 12 3905863 2 2007 4 18 10 20 000000 000 30 50 3905863 2 2007 4 18 13 10
24. 0 10000 QAquick gel e raction Qageng HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH Escheri chi a col i 000 000 0000 0000 000 F ACSVant SE Becton Di 20 Si havy et al Experi ne nts wth gene fusions Cold Spring Harbor Laboratory Cold Spring Harbor N Y 1984 0000000000000 0000000000000 OD HHHHHHHHHHHH 00000 30 18 3905863 B2 2007 4 18 DNA Genbank ORF AL646069 124 Ralstonia solanacearum 70
25. 86 82 AF361470 21 Rhizobium leguminosarum AL646058 49 Ralstonia solanacearum 016788 268 Pseudomonas putida AF416331_47 Ruegeria sp RalstonialHHHHHHHHHHHHHHHHHHHHHHH RalstonialHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH C PD mm TL PTS rn m P DPT FISSE TTT nnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnn HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
26. ca acatttccgt gicgecctta tteccttttt tgzcgzgcattt tttttgzctca cccagaaacg ctggtgaaag taaaagatgc gaagatCag tteggigcac gagtgggtta CatCgaaCtg gatctcaaca gcggtaagat ccttgagagt tttegccccg aagaacgttt iccaatgatg agCaCtttta aagticigci atgtggcgcg gtattatCCC gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagicaca gaaaagcatc ttacggatgg agagaattat gcagtgctgc 2007 4 18 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 10 20 30 22 3905863 2 2007 4 18 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggzatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcglgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg Cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cegataaagi tgcaggacca cttctgcgct cggcccttec geciggcigg tttattgctg ataaatctgE agccggtgag cgtgggtctc gcggtatcat igcagcactgz gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag icaggcaact atggatgaac gaaatagaca galcgctgag ataggzigcct cactgattaa gCattggtaa cigtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat ccititigat aatctcatga ccaaaatccc ttaaCgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcgZtggtt tgtttgccgg atcaagagct accaactctt
27. cggaaaact tacccttaaa cacttgtcac tactttctct aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactataa gcttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc aaaggtattg attttaaaga agatggaaac aticttggac aactcacaca atgtatacat cactgcagac aaacaaaaga aaaatiagac acaacattga agatggaagc glicaactag actccaattg gcgatggccc tgtcctttta ccagacaacc gccctttcga aagatcccaa cgaaaagaga gaccacatgg gcigciggga ttacacatgg catggatgaa ctatactaag cgtcgtttta caacgtcgtg actgggaaaa ccctegcgtt agcacatccc cctttcgcca gctgegcgtaa Lagcgaagag ttgttaatag aatcgagtta acaaaltgga atacaactat atggaatcaa agCtaacttC cagaccatta tcaacaaaat attacctgtc cacacaatct iccttcttga gitigtaaca catgcaagct tggcactggc acccaactta atcgccttgc gcccgcaccg atcgcccttc 300 360 420 480 540 600 660 120 180 840 900 960 1020 1080 1140 10 20 30 21 3905863 2 ccaacagttg cgcagcctga atggczaatg gcgcctgatg cggtattttc tCCttaCgCa tctgtgcggt atttcacacc gCatatggtg cacicicagi acaatctgct CIgatgCCgC atagttaage cagccccgac acccgecaac acccgctgac Ecgccclgac gEgCttgtct gctcccggca tccgcttaca gacaagctgt gaccgicice gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcECEag acgaaagggc ctcgtgatac gectattttt ataggitaat gtcatgataa taatggittc ttagacgica ggigecacit ttcgggzgaaa igtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatet gagaCaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtatt
28. ctetgtagc acegcetacataeetegetetgetaatectgt Laccagt BgC Lire LEO agtggcgataagtcegtgtettacegggttgga ctcaagacgulegltaceggal Aaggegcapeagtegegcisancegegggi teal gcacacagcccagettggagcgaac R cctacacogcactgegatacctacagcgtgagctetgageaagepeeacgetteecgaagggagaaaggeggacaggta LLeonggggganacgeetgetatetttatagtecigi caggiliegecacotetgaciigageglegattiligtgatgotegtcasggesgeggarectatggasaazegecagcaa cgcgecetttttacgglicctggecttttgetggecttttgetcacazgtteztt Egcgttatcccctgettctgtgga laacegtattaccgecttteagtaagctgataccgetegecgcagccgaacgaccgageacagegagteagtirancgagga ageggaaga Acquisition Histogram Plot 15 20 25 30 ES 10 50 10 197 105 104 A Acquisition Histogram Plot 8 Rs Da s v 90 10 105 10 24 3905863 B2 2007 4 18 0000 BamH I ggatcctaattaattaag G S P_ N 0000 Acquisition Histogram Piot 1 15 20 25 50 5 10 0 100 ROC 0000 Acquisition Histogram Plot 10 15 20 25 30 5 10 102 105 10 B Acquisition Histogram Plot 15 20 25 30 10 5 109 10 105 10 102 25 dLB tion Histogram Plot
29. ganagggcetcetgatacgectatttt talaggi Laatgteatgat aataatggtttcttagacgtcaggtggcactttiegeggaaatgtgcgcggaaccectattigtttatttttcieaataca Licagatatgtatccgctcetgagacaataacecctgatasatgeticaataatat acattteegtglcgeccllattrccttttttgcggcatltlgccticctglutlttmctcacccagpaaacgcteglgaaagt aaaagalgelgaagatcagtigegigcacgagigggitacatogractgratcicaacageggiaagatocttgagagitl le amp ccecgangaaegltttecaatgatgagcacttttaaagttetgotalgiggcgcggtattatecegtat tgacgccEE geaagagcaactcgglegccgealacactattetcagaatgactt amp gLteagLactcaccagtcacagaaaagcatet ac SEalggcalgacagtaazagaattatgcagtgclgcentaaccatgagliataacactgcggecaacttacttetgacaac gatcggagraccgaangpgage ilaaccgett itgcacaacatggeggatcatgtaactegecttgategttgggaaccgga gctgaatgasgccataccaaacsacgBagcg gacaccncgatgecrgtapcaalggcaacaacgt gegeaasactat taac iggcgaactacttacictagell EgcaacaattaatagactggalggaBgcegataaagttgcaggaccactictgeg cteggecettecageiggotegtilattgcigninaatciggagecggigagestgegicicgcggiatcaltgcageaci sesgccagal egtaageccicerglalUglagttatclacacgacggggagicaggeaactatezalgancgaaatagaca galcectgarataggigecteacigatlangenttggtancigtragaccaagiilacicatalaraciltugatigatti aaaactteatttLtaattlaaasaggatctaggtmaagalecttttcgataatetcatgaccaaaateccitancgtgagt t tegttecactgagegteagacccegtagaanagatenaaggatcttcitgagatectttitl tetpegegtaa et geg Citgcaa caanaanaccaccgetaccageggtggi lgecggato gacctaccaaoetcttLttecgaaggtaac EBCtLCagcagagegcagataccaae Lact gtecttetagtgtagecgtagi Laggecaecacticaapaa
30. ti on ki t Q agen Escheri chia coli 0 15 3905863 2 2007 4 18 jigugggugugaagauauggugugugaagauauggauuguugauuuuuuuuuu F ACSVantage 5 Becton Di Sil havy et al Experi nen ts Wth gene fusions Cold Spring Harbor Laboratory Cold Spring Harbor N Y 1 984H HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 10000 Hino S et al Phenol hydroxyl ase cl one d from Ral stoni eutropha strain E2 exhi bi ts novel kinetic properties M crobi ol ogy 1998 144 1765 72 O 0000 1000 000000000
31. tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac CagtggCtgC 2040 2100 2160 2220 2280 2340 2400 2460 2520 2580 2640 2100 2160 2820 2880 10 20 30 23 3905863 2 igccagtggc gataagicgi gtettacegg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggtte gigcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgzagaa agcgccacgc ttcccgaagg Egagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagage gcacgaggga gettccaggg ggaaacgcct ggtatcttta tagtCCtgtC gggtttcgcc aCCtCtgact igagcgtcga tttttgtzat gctegtcagg gggzcggagc ctatgzgaaaa acgccagcaa cgcggccttt ttacggttec tggccttttg gctcacatgt tetttectgc gttatcccct gattcigigg ataaccgtat taccgccttt gagtgagCtg ataCCgCtCg acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 2007 4 18 2040 3000 3060 3120 3180 3240 3300 3360 3411 10 20 30 p18GFP 3411 bp
Download Pdf Manuals
Related Search