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1. I 1 2 3 4 6 5 EN61010 Srp HHL TOE I 1 1 1 2 2 2 1 J D Y2. 2 mm BBV TABBY 2 5 cm o
3. 135 0016 RRITARREI 2 2 20 2008 1 For Research Use Only ed For safety this manual must be read carefully and the device be handled properly g Instruction Manual Cassette Electrophoresis Unit O O Model DPE 1020 5 Cat No 303111 Pat No JP1802133 US4772373 EP0224194 DE GR lt Top View gt lt Side View lt Front View gt TIL Specifications Cautions SS 7 Lid Acrylic This electrophoresis device must be operated together with an external Anode Buffer Chamber Acrylic DC power supply designed specifically for electrophoresis applications Cathode Buffer Chamber Frame Acrylic under a high voltage The output of this power supply must be isolated Gasket Silicone from external ground to insure that the DC voltage output floats with Wedge Acrylic respect to ground Acrylic Plate Acrylic This device is designed to meet EN61010 1 safety standards If this device Overall size Lx W xH 8x 18X18 cm is used or modified in a manner not specified in this manual then protection Weight before buffer filled 0 5 kg afforded by the device will be impaired Alteration of this device will void Precast Gel Compatibil
4. MODEL DPE 1020 30311 Pat No JP1802133 US4772373 EP0224194 ee 4i EE CORAL L ST CHAR STIS Al z A EA N AD we 0 600 V 0 100 mA O 50 FAROR A RATP ER A EMAL TEE O WE lt in vitro KEEDA UF RRE 1
5. 10 13 SDS PAGE 1 30 mA ALE 60 Native PAGE 1 15 mA 100 DNA PAGE 1 15 mA 100 BPB FF 5 mm a RAYS Oo Fe ERY SKEET HBS Lo
6. 2 9 GS 7 7 A 4 cm 200 mL 1
7. of one year from the original purchase date DPC will repair exchange the defective parts under this warranty subject to the terms and conditions of repair of Daiichi Pure Chemicals provided that the device is operated according to the manual and labels 2 Slide the Wedges towards the center of the Outer Chamber Anode Buffer Chamber to release the Inner Chamber of Acrylic Plates and Cathode I Features Buffer Chamber Frame 1 Unique Wedge system to firmly and easily fix the Gel Plate 3 Take out the Inner Chamber 2 Buffer filled in the Chambers to cool all the surface of the Gel Plate 3 Smaller quantity of buffer required Y Assembly and Operation 4 Easier sample loading visible through the transparent body 5 EN61010 1 safety standards satisfied Caution Electrophoresis operates at a high voltage and glass made Gel Plates are fragile by nature some protective measures like wearing gloves II Components should be taken when handling DPE 1020 not to injure yourself hands etc Description Quantity 5 Ae rone eme 1 Ensure that the power supply is turned off and the Lid is not connected 3 Cathode Buffer Chamber Frame with electrodes 1 2 Ensure the Gaskets are set on the Inner Frame Cathode Buffer Chamber 4 Gasket 2 Frame Otherwise place the Gaskets on the grooves of the Inner Frame 5 Wedge 2 and press the Gaskets so that they are fixed firmly into the grooves 6 Acrylic Plate 2 3 Pour th
8. ba CI H the BRER y 7 7 PH LXWXH 8X18X18 cm ik 0 5 Kg II 600 V 100 ETN HERS y 7 po dik 500 mL BARS COMM ee aR RUE Ce it 1 Mey 2p A EN 2 3 2H V FFA
9. e buffer approximately 200 mL in the Outer Chamber Anode Instruction manual 1 Buffer Chamber up to around 4 cm from the bottom Then place the Inner Frame into the Outer Chamber matching respective colors of the plugs red and black to those as indicated on the Outer Chamber g 9 10 11 12 13 14 Take out a Gel Plate Please refer to Related Products from the bag and remove the comb gently Insert the Gel Plate s placing the grooved side facing towards the inside of the Inner Frame If only one piece of Gel Plate is placed put the Acrylic Plate on the other side of the Inner Frame to fully build the Inner Chamber Stabilize the Inner Chamber by inserting the Wedges straight down into the corners between the Gel Plate s or Acrylic Plate and the Outer Chamber Note Place the grooved end of each Wedge facing outside otherwise the Lid cannot be set place Do not insert the top of the wedge below the top of the gel plate Inserting the wedge too far can warp the gel plate causing a gap between the glass plate and gel Fill the Inner Chamber with running buffer up to 2 mm below the top edge of the Gel Plate s After a few minutes check for leakage lowering of the level of the inside buffer If leakage is observed disassemble the Inner Chamber and repeat the above steps Note Avoid a short circuit do not overfill the Inner Chamber Fill the Outer Chamber with running buffer up to 2 5 cm below the top
10. edge of the Outer Chamber Note Pour the buffer gently to avoid trapping air bubbles along the bottom of the Inner Chamber Minor bubbles can be ignored Load a sample into the well Please refer to the Instruction Manual of Gel Plate for preparation of samples Ensure the power cables of the Lid are not connected to the power supply Place the Lid on the Outer Chambers and slide it so that the plugs can be connected with the jacks firmly Connect the power cables of the Lid to the power supply with the proper polarity Note When attaching the Lid the power supply must be off Turn the power supply on to start electrophoresis Recommended conditions for your reference SDS PAGE 30 mA DC constant for about 60 minutes a Gel Plate Native PAGE 15 mA DC constant for about 100 minutes a Gel Plate DNA PAGE 15 mA DC constant for about 100 minutes a Gel Plate Turn the power supply off to stop electrophoresis when the tracking dye Bromophenol blue or Xylene cyano FF reaches approximately 5 mm around the center of Gasket from the bottom edge of the Gel Plate s Disconnect the power cables of the Lid from the power supply Holding the device firmly slide the Lid as indicated on the top for open Discard the buffers and release the Gel Plate s by sliding the Wedges towards the center of the Outer Chamber Staining or other procedures to follow the electrophoresis should be operated according to t
11. he individual instructions The device should be washed after each run with a gentle detergent and rinsed thoroughly with distilled or de ionized water COSMO BIO Co LTD URL http www cosmobio co jp DPE1020 0107
12. ity MULTIGEL I mini Gels the EN61010 1 safety standards warranties and create a potential safety Electrical Limits 600 Vde and 100 mA hazard Buffer Chamber Capacity 500 mL Daiichi Pure Chemicals is not responsible for any injury or damage caused by use of this device for purposes other than those for which it is Note This device is not compatible with acetone or ethanol Use of organic intended or by modifications of the device solvents voids the warranties The electric operating parameters for this device are as follows Voltage Limit 0 600 Vde Additional equipment and materials required not included Current Limit 0 100 mA Power supply Ambient Temperature Limit 0 50 C i Electrophoresis buffer Please refer to Related Products Protective gloves and safety glasses must be worn when operating in a Sample loading solution Please refer to Related Products laboratory environment This device is intended for in vitro use only and must be used with its Lid whenever it is operated 7 Gel Plates that are separately available Please refer to Related Prod IV Unpacking ucts are made of glass The Glass Made Plates are breakable if pressed 1 To remove the Lid hold the device firmly and slide the Lid as indicated on improperly when attached or detached Plates should be set again into the the top device when difficult to be assembled properly Limited Warranty This device is warranted against manufacturing defects for a period
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