TaKaRa - Anatomy
Contents
1. 1 2 DNA PCH 8 10 12 15 kbp 2 Genomic DNA PCR 0 5 1 2 4 6 8 kbp 5XPrimeSTART Buffer Mg plus 1 mlX2 Mg 5X 5mM 20 dNTP Mixture dATP dCTP dGTP dTTP PCH 800 2 5 mM pH pH7 9 98 20 PCR Total 50D 5XPrimeSTAR Buffer 10 dNTP Mixture 2 5 mM each 4 Primer 1 0 2 0 3 p M final conc Primer 2 0 2 0 3 p M final conc Template lt 200 ng PrimeSTARY HS DNA Polymerase 2 5 U pl 0 5 upto 50 gl PCR Tem2. PrimeSTAR HS DNA Polymerase Code No R010A Shipping at 20 C Size 250 units Stored at 20 C Lot No PrimeSTAR HS DNA Polymerase PCR 3 5 exonuclease 7ag DNA Polymerase DNA Polymerase 3 5 exonuclease PCR Fidelity cDNA Fidelity PCHR PrimeSTAR HS DNA Polymerase 2 5 units gl 5XPrimeSTAR Buffer Mg plus 5Xconc dNTP Mixture 2 5 mM each 50mM Tris HCI pH8 2 at 4 100mM NaCI 0 1 mM EDTA 1mM DTT 0 1 Tween 20 0 1 Nonid
3. et P 409 50 Glycerol DNA 74C 30 10 nmol 1U 100 mM Tris HC pH8 3 at 37 10 mM KCI 2mM MgClz 6mM NH4 z2SO4 0 1 TritonX 100 200 pM dATP dGTP dCTP 100zM FEH TTP 0 001 BSA 0 4 mg ml DNA 1 10U 0 6 g A Hind 74 1 DNA 2 10U 0 6 g supercoiled pBR322 DNA 74C 1 DNA Polymerase Chain Reaction PCR DNA ese ee eo ee oe ooo ooo ee ee eos eee ooo eo noo oe eee oe oo ee TakahRa 3 4 1 520 2193 http Awww takara bio co jp PCH PrimeSTAR HS DNA Polymerase PCH PC
4. plate Genomic DNA 5ng 200 ng lt 200 ng E coli Genomic DNA 100 pg 100 ng ADNA 10 pg 10 ng DNA 10 pg 1 ng PCH 98C 10 sec 55C 5sec 15sec 30 cycles 72C 1 min kb or 98C 10 sec 30 cycles 68C 1min kb PCH ee Tel 077 543 6116 TaKaRa A 1 Fax 077 543 1977
Download Pdf Manuals
Related Search