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1. AE 1475 ExzBlockChemi AE 1476 ExzBlockBSA BSA BSA AE 1477 ExzBlockCAS CAS WSB 7200 ExTBS WSE 7235 EzTween HRP lgG Xx1 1000 TTBS 3 AE 1490 ExzWestBlue TMB AE 1495 EzWestLumi pluse 3 1 Tween 20 2 A 1 p 3
2. A a HC 3 PVDEF polyvinyliden difluoride
3. CB B CB 0 001 CBB 10 30 2 10 509 1 CBB 1 10 1 100 2 5 0 109 309 100mL 45mL 52 CBB ty ES 5 10 1 lt MES A 2
4. 1 0 0 1 Tween 20 Triton X MR P EE 9O2gm 0 2um 0 9mm 2
5. 3 7 5 CC 2 DC 10 2 20 30 2 POD HRP XX CCD
6. 1 NN 6 6 0 01 0 02 SDS 9 0
7. 4 6 3 6 3 5 2 09 PVDFE 5
8. 6 6 1 1 6 3 To wb in 6 6 3 2 0
9. P PVDEF PVDF 2 lt 4 2 gt P PVD F 1 2
10. CCD gt ooo E9300 Ez MG Alb HRP 8 InonRI AE 6530P WSE 3200 E T520L SDS PAGE SDS PAGE SDS PAGE e AE 1430 FeApply AE 1410 EzRun MO 13 800
11. 2 5 Kyhse Andersen J 1984 J Biochem Biophys Methods 10 203 209 6 2 2 20 10 5 PVDEF 3 10C 209 6 3 3 PVDF
12. 2 0 1 Towb in 100mM 25mM 192mM 59 20 AB 1460 EzBlot A 0 3M 5 B 25mM 5 Wo 40mM 6 5 nnnm DF B 144mA 2 mA cm2 B 1 25 35v 30 A 2 6677S
13. PVDF 00 6 4 1 2mA cm2 30 9 0 6 1 6 2 6 6 6 5
14. 6 800 4 800 WSE 4020 WSE 4040 WSE 3500 3 AE 1460 AE 1465 2M R 4M R Blot ExzFastBlot HC 12 000 9 000 TBS HRP HRP AE 1475 ZockOegy77 WSE 230 EzTBS E 1490 WestBlue UNE 120 Westlumipluse AE 9300Es Capture MG 9 000 5 000 12 000 22 000 _ Mb 29198 a gt t vV fT TT DNA 111 0041 3
15. 1 9 7 9 DNA Southern Towb in 1984 Kkyhse Andersen Kyhse Andersen J 1984 Biochem Biophys Methods 10 203 20 9
16. AE 8450 1000VC A lt C C 100 ml 1 lt 2 1 PVDF PVDF B 3 0 6 5 C 2 mA cm2 144mA 50V 30 40
17. 2 2 03 5827 4861 03 5827 6647 03 5827 4873 03 5827 4874 000 1 06 6136 1421 05 6356 3625 5F Q 110 0016 2 81 5 03 5818 7560 03 5818 7563 EURL http www atto co jp e mail iinfo atto co jp
18. 4 BIOSCIENCE amp BIOTECHNOLOGY Western Blotting Since 1964 50 me AN SupportingLifeResearch 1 Western Blotting 1
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