リアルタイム PCR（Probe 法）実験ガイド －目次－ 1 蛍光
Contents
1. RNA PCR Probe 1209 2 PCR 2 Thermal Cycler Dice Real Time Thermal Cyeler Dice Real Time Quick Manual 3 1 3 1 PCR 12 1 12 2x CycleavePCR Master Mix 12 5 pu1 150 1 5X Primer Probe mix 5 1 60 m1 dHsO 2 5 1 30 1 12. PCR Probe 1209 1 2 Pi 3 3 T KN w F 2 920 200 1000 1 Ll Vortex 1 5 ml 8 0 2 ml PCR Thermal Cycler Dice Real Time System Thermal Cycler Dice 48 Real Iime System Lite TP700 PCR Probe 1209
3. PCR PCR IC Internal Control x amp PCR FAM PCR ROX umn Gt FMMRNM RN Positive Control Plasmid PCR Positive Control Plasmid
4. CY210 Positive Control Plasmid Positive Control Plasmid 17 1 cfu PCR Probe 1209
5. dH20 5 3 3 Q 51 PCR 4 PCR 2 PCR PCR PCR Probe 1209 Positive Control Plasmid EASY Dilution
6. PCR Probe Probe PCR R OO MN PCR Probe 1209 1 RNA DNA PCR RNase H RNA RNA RNase H OO SNPs RNA
7. DNA TE 2 3 RNase H RNase H TR 4 WE 5 5 sr 5 5 8 TaqMan TaqMan 5 3 DNA Taq DNA 5 gt 8 TaqMan
8. PCR CycleavePCR Listeria monocytogenes inlA gene Detection Kit CycleavePCR Campylobacter u77 co Typing Kit CycleavePCR Staphylococcus au7eus DnaJ gene Detection Kit CycleavePCR 6 CycleavePCR Legionella 5S rRNA Detection Kit Ver 2 0 CycleaveRT PCR Cryptosporidium 18S rRNA Detection Kit CycleaveRT PCR Giardia Ditection 18S rRNA Detection Kit TaqMan TaKaRa qPCR Norovirus GI GII Typing Kit 3 1 2 DNA DNA DNA DNA RNA
9. 1 1 Xx 10 copies ul Positive Control 2 1 Xx 10 copies u Positive Control 5ul EASY Dilution 45u 3 1 Xx 10 copies ul 2 1x10 copies u 1 5bhl EASY Dilution 45u 4 1X102 copies L 3 1x10 copies u 5ul EASY Dilution 45u 5 10 copies L 4 1X10 copies u 5bul EASYDilution 45h D 6 1 copy L Il 5 10 copies u l 5ul EASY Dilution 45h 1 1 x 10 1 x 109 4 Ct PCR 2 I a PCR MMS 0oRo Ct Ct Ct Ct 40 PCR Probe 1209 PCR
10. hermal Cycler Dice 96 Real Time System 7 TP900 PCR Thermal Cycler Dice Real Time System 8 96 i TN individual Flat Caps NJ600 96 Sealing Film for 96well Hi Plate for Real Real Time Time NJ500 NJ400 48 24 well 2 48 48 well snap plate NJ700 Flat cap for snap plate NJ720 PCR Probe 1209
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