foodproof STECスクリーニング検出キット 取説抄訳
Contents
1. Tel 043 242 0425 Fax 043 242 0445 E mail sales practicaljp Web site www practicaljp I2 E H RACTICAL BIOTECON Diagnostics 4 STEC DNA FAM HEX ROX Cy5 DNA STEC DNA DNA2. Tel 043 242 0425 Fax 043 242 0445 E mail sales practicaljp Web site www practicaljp I2 E H RACTICAL BIOTECON Diagnostics References 1 Karch H Tarr P Bielaszewska M 2005 Enterohaemorrhagic Escherichia coli in human medicine Int J Med Microbiol 295 6 7 405 18 2 ISO TS 13136 2012 Microbiology of food and animal feed Real time polymerase chain reaction PCR based method for the detection of food borne pathogens Horizontal method for the detection of Shiga toxin producing Escherichia coli STEC and the determination of O157 O111 O26 0103 and 0145 serogroups 3 FSIS Microbiology Laboratory Guidebook MLG 5B Detection and Isolation of Non O157 Shiga Toxin Producing Escherichia coli STEC from Meat Products 2011 LightCycler 480 5 Supplementary Information 5 1 Ordering Information BIOTECON Diagnostics is offering a broad range of reagents and services For a co
3. STEC DNA PCR 1 PCR PCR stx1 VT1 stx2 VT2 eae 2 TaqDNA 5 PCR 5 3 3 TaqDNA PCR
4. EHEC STEC HUSEC VTEC 1 ETEC EPEC EIEC EAEC DAEC stx1 stx1c stx1d stx2 stx2c stx2d stx2f stx2g ISO TS13136 iX 2 USDA FSIS method MLG 5B 3 stx eae x m
5. auto ROX 3 FAM HEX ROX Cy5 Cy5 25uL 5 L 2 8 DNA DNA DNA
6. PCR DNA DNA BIOTECON DNA DNA DNA PCR
7. Tel 043 242 0425 Fax 043 242 0445 E mail sales practicaljp Web site www practicaljp I2 EF E RACTICAL BIOTECON Diagnostics 2 3 stx1 VT1 FAM stx2 VT2 HEX eae ROX Cy5 FAM Stx1 VT1 HEX Stx2 VT2 ROX eae Cy5 FAM HEX ROX Cy5 stx1 stx2 eae stx2 eae stx1 eae RE stx1 stx2 stx2 stx1 STEC eae STEC eae ES NOTE FAM HEX ROX Cy5
8. 1 PCR NOTE ABI7500 Strip LightCycler480 Strip LightCycler480 1 12 PCR
9. DNA 2 foodproof StarPrep One Kit Magnetic Sample Preparation Kit Tel 043 242 0425 Fax 043 242 0445 E mail sales practicaljp Web site www practicaljp I2 E E RACTICAL BIOTECON Diagnostics foodproof amp STEC Screening LyoKit 5 Nuclease DNA FAM HEX ROX Cy5 PCR LightCycler amp 480 LightCycler
10. PCR m jj 6 PCR
11. 4 PCR UNG Uracil DNA N Glycosylase PCR dUTP deoxyuridine triphosphate PCR UNG UNG DNA PCR UNG DNA DNA Uracil dTTP dUTP UNG
12. 96 DNA Taq DNA 8 x12 Uracil DNA N RP Regular Profile Tube 0 2mL 2 lt 8C LP Low Profile Tube 0 1mL Control Template 1x250 uL DNA 2 C 8 C H O PCR grade 2 x 1mL PCR PCR 2 C 8 C 8 x12 FAM HEX ROX Cy5 PCR PCR
13. Tel 043 242 0425 Fax 043 242 0445 E mail sales practicaljp Web site www practicaljp
14. Tel 043 242 0425 Fax 043 242 0445 E mail sales practicaljp Web site www practicaljp E E RACTICAL BIOTECON Diagnostics 2 2 PCR FAM stx1 gene HEX stx2 gene ROX eae gene 3 Cy5 internal contro POR Pre Incubation 1 cycle Step 1 37C 4 Step 2 95C 5 Amplification 50 cycles Step 1 95C 5 Step 2 60C 60 Fluorescence Detection Step 2 Cooling 1 cycle 40 C 30 NOTE PCR passive reference dye probe quencher non fluorescent dark quencher passive reference dye Agilent Mx3005P 0012 amp Instrument Filter Set Gain Settings FAM Filter Set Gain Setting x 1 Roche
15. amp 96 Roche Diagnostics ABI 7500 Applied Biosystems Mx3005P Agilent Technologies PikoReal amp 24 Thermo Scientific 2 2 1 PCR DNA PCR EDNA PCR PCR PCR PCR
16. I2 E 3 RACTICAL BIOTECON Diagnostics foodproof STEC Screening LyoKit 5 Nuclease R 602 11 1 R 602 11 2 Ver 1 Sep 2013 3216D60211 foodproof STEC by 2014 11 26 1120 H26 VT DNA 1 PCR 96 1 94 B 2C lt 8C B foodproof STEC Screening LyoKit stx1 stx2 eae
17. LC480 LC480 FAM 483 533 HEX 523 568 ROX 558 610 Cy5 615 670nm LC480 II FAM 465 510 HEX 533 580 ROX 533 610 Cy5 618 660 nm PCR NOTE PCR 1 2 NOTE 25 L BUB DNA 25 L PCR 25 L PCR 25L Control Template 25uL NOTE
18. hts are conveyed including no right to use this product for in vitro diagnostic therapeutic or prophylactic purposes Further information on purchasing licenses under the above patent may be obtained by contacting the Licensing Department Life Technologies Corporation 5791 Van Allen Way Carlsbad CA 92008 Email outlicensing Iifetech com 5 3 Trademarks foodproofeis a trademark of BIOTECON Diagnostics GmbH Other brand or product names are trademarks of their respective holders 5 4 Contact and Support If you have questions about this or any other product of BIOTECON Diagnostics please contact our Technical Support staff for details see www bc diagnostics com Our scientists commit themselves to providing rapid and effective help We also want you to contact us if you have suggestions for enhancing our product performance or using our products in new or specialized ways Such customer information has repeatedly proven invaluable to us and the worldwide research community 6 Change Index Version 1 September 2013 First version of the package insert
19. mplete overview and for more information please visit our website at www bc diagnostics com 5 2 License License Notice NOTICE TO PURCHASER LIMITED LICENSE Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 804 375 5 538 848 5 723 591 5 876 930 6 030 787 6 258 569 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product solely in Food Testing Applications and Genetically Modified Organism GMO Testing Applications including reporting results of purchaser s activities for a fee or other commercial consideration and also for the purchaser s own internal research No right under any other patent claim is conveyed expressly by implication or by estoppel Further information on purchasing licenses may be obtained from the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA The purchase price of this product includes limited nontransferable rights under U S Patent No 7 687 247 owned by Life Technologies Corporation to use only this amount of the product to practice the claims in said patent solely for activities of the purchaser for bioburden testing environmental testing food testing or testing for genetically modified organisms GMO in accordance with the instructions for use accompanying this product No other rig
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