テーマ4．鳥インフルエンザウイルスの実際
Contents
1.2.
3.
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5. a 7 26 13 30 17 00 28 28101 26 1 13 30 13 45
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14. 1 A B C 1 2 39 Wr ET ICTVdp CH gt S NH 50 1945 1955 1965 1975 1985 1995 2005
15. 2 2OWW
16. 0 RT PCR PCR
17. 4 C 55 5 Tel 084 936 2111 4047 a Fax 084 936 2023 e mail nakaura fubac fukuyama u ac jp Fax 084 957 3422 Fax 084 957 3422 Tel 0865 65 3922 Fax 0865 65 3922 rel 084 936 2111 51239 Fax 084 936 2024 e mail hirose fupharm fukuyama u ac jp 4 4
18. 4 C 49 28 lt
19. 4
20. ADI 4a1l
21. 16 AI AI
22. 4 a 13 Hazard Idantificatinn Release Assessment Exposure Assessment Consegquence Assessment Exposure Hazard Assessment Characterization Risk Characterization Risk Estimation 1 No 19 15 BSE a 2008 4 ma a 4
23. 10 4 O 18 2 1 PJ2 2 6 2 0 3 3 1 3 2
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25. 2008 11 20 aa 11 11 15 SGD a a AI GD GD AI
26. 2 7 EHSA N SN se gt Se 4b5 3 1 AGP 1 HE
27. 3 1 2
28. A 7
29. 3 1 URL 0 9 9
30. F to 2 gt I ee ii 1
31. 1 1 9 1 6 gt H5N1 1 1 3 2 1 1 4 2 3 2 2 4 lt 1 7 gt H5N2 6 1 4 1 578 1 9 gt H5N1 1 2 3 6 1 1 1 7 26
32. 30
33. L L 3 REACH EU Ei B ha CR ET
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36. 3 WHO 4 17 4 4
37. 4 14 1 2 3 4 4 1 Faze3 4 2 Faze4 1 2004 79 1 2 3 iii ir _ 2 12 9 RN gt
38. 1 TSN2 1 0 HRN bis EE D4V5 HN WIIO 0OI 0 gt 200NMC 1 7 26 Yi Z008 6 19 385 Z43 4 C 16 7 26
39. ELISA ELISA CG 2 A AIV QNP M AGP AIV
40. 4 C 20 0 31 1 rs 1
41. _ gt 44 9 2003 10 a i pa a e Ew 2 I gt Ws by ee AT LU 7 IIND HSN2 HENS HSN1 LHIEN 1 C D HSN1 0 SR i HSN1 4 34 6
42. 4 C 50 D
43. O 4 8 1 5 4 8
44. I Hr me mm t ee I pa ni pbh ea EE i FE rr Ti ie Thr ee 1 i hi En i PF Pr rg i EE rk le ils a Tk a Hr 5 SrSE EE 48 p92 2 6 it
45. 11 Avian Influenza Ai e H7 H9 H5 8 Avian Influenza Ai e e e 10 Avian Influenza AI
46. NA a pr Hg 3 D 8 PO DH 2 A 7 26 4 11 NXsTSTeIe 7 26
47. 7 RI H5N1 1997 er SI my NINE E 2 8 RNA 1 28 256 HINI 19 1 8 H3N2 1968 LT a J i 1 0 4 0 20XX 4 700 Z2500 1710 H5NI 4 65
48. 3 Diseases of Poultry 11 Blackwell OIE FAO 2004 9 28 4 a 44 7 eeeeeeee J v 3 0 0EA0 Al 100 eNSA frrIoN O AdNNTT r Iv 2 wS HSNVY d C
49. Os 1 2004 2007 2
50. 1 2 3 4 4 4b3 2
51. 60C 4 61 60C 10 BSE 133C 3 20 70C 1
52. 2004
53. AI 4 4 a37 1 AI AI AI 9 2 1 2 4 438 3 1 2 3 1 IV AI 1
54. 4 Cc 47 URL Or 1 E pe gt Dp af uh C 1
55. oy lt gt 0 D Dn FE _ gt 3 2 7 26 0 rn 4c 19 29 7 26
56. 1 2 3
57. F gt 2 aN Rn 3 Ne i OA ee A 0 7 26 4 C 8
58. 2 PJ2 4 20 7 26 4 FE 4 a 1 4 a 72 4 a 3 4 A 1 4 a 6 4 a 14 2 4 a 15 4 a 24 3 4 a 25 4 a 50 4 4 a 51 4 a 68 4 a 69
59. NEDO 4 6 4 H5 H7 A 2004 2004
60. H5N1 4 12 2 1 a 6 7 301 30 b 8 18 18303 10 c 9 10 301 10 d 2009 2 903 100 20
61. 10 000 Al AlI Al AlI Al AI
62. DNA 100 DNA 4b8 1 DNA 2 nr n DNA DNA DNA PCR 1st 5 2nd 2nd cDNA mth 4b9 RNA PCR PCR RNA DNA
63. 7 Ba 2 2 ee 1 2 NM Us 084 957 3414 0648 22 2052 0648 66 2114 0666 62 0824 064 936 2111 4616 064 936 2111 4047 084 928 1031 0824 72 3127 0648 22 2052 0648 68 0011 0665 65 2566 0665 65 2566 0665 65 2566 0624 64 6215 064 936 2111 FAX 064 957 3422 0648 24 7555 0648 66 2532 0666 63 1552 064 936 2023 064 936 2213 064 927 7021 0824 72 3531 0848 22 3340 0848 68 0053 0
64. 5 Avian Influenza AlI e e e 4 C 26 2 Avian InfluenzZa Ai A A B C A A N 16 H1 16 N 9 N1 lt 9 4 Avian Influenza Ai e
65. 4 BSE
66. 12 1 100 le 10 100 e e e 3 e 4 SE SE 5 AI 4 C 35 4
67. 4b 4 ELISA RT PCR RT PCR RT PCR
68. DRNA 1x10 1X1 XI 1X10 DNA PCR DNA SYBR Green PCR 2 0 4b19 Roche DNA RNA 329 3 gt ECR 5 0 5ml 1 5 1 999 1 2 5 90 1
69. Sb 00 GE CC HW RT PCR DNA RNA 4b15 4 NF A 1 NP1200f CAG RTA CTG GGC HAT AAG RAC R Aor G6 H A or T or C NP1529r GCA TTG TCT CCG AAG AAA TAA G
70. amp 21 2 200 PJ2 KK 4 5 2008 7 26 19 23
71. 4 C 53 9 15 30 2 3 1 2 3
72. 4b10 PBS RNA RT PCR 2 2 OR 20XTAE 0 2 59 TI 0 2 DO CT ZI LM ED A 900 2m1 PBS pH7 2 3000r pm 5 4 140 1 RNA RNA RNA QIAamp Viral RNA Mini
73. RT PCR RT PCR CA A Ce gg L gt RNA 2 PCR polymerase chainreaction DNA 20 30 60 DNA 100 pia DNA DNA DNA DNA DNA RNA 3 se ee ee ee DNA PCR
74. ss J 7 26 4 C 13 1 a F 7 h 1 2 A
75. s e 4 C 27 4 C 28 2 3 QD J CD 3 2 2 3
76. 2 3 1 2 4 5 3 6 HP gs
77. AIV 3 37 30 3 000 rpm 10 3 1 000 G 0 1 4b6 8 w v NaC1 0 1M PBS pH7 2 1 w v 2 3mm 20 1 1
78. FN HSN 1 hE TL Hu a el WiO OI 2008 6 11 Z008 6 19 385 Z43 4 5 WHO H5N1 3008 5 38 WHOD sh E 2 mo ll of of of sl of of of ol ol wm 0 0 0 0 9 9 90 9 9 9 1 9 1 cmg 6 of 4 4 il dl lr WW el al ml ml sf sf sl sg o of of of of of of of of of 1 1 gt 0 9 0 9 0 9 Bl to 25 9 7 3 0 9 0 0 20 13 5 45 42 397 6 13 0 9 of 9 of 9 3 2 9 9 of of 3 lt of of of 9 ol 2 2 9 6 0 0 9o 9 9 9 9 1 9 eo oo 0 of of 9o ol ol ol ii ii of ri 0 9 90 9 9 9 of 9 3 1 0 9 9 9 7 5 2 3 3 of of of of 7 FWa ml li Wi li ml 4 Wi 9 4 3 3 29 2 el 9 90 9 8 5 5 5 52 4 4 46 32 98 9 79 88 99 2 24 8 2
79. RT POR 4 13 21 RT PCR
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81. 1 4 a 62 100 1 2 2 3 4 4 4 63 H5N1
82. WHO 70C 3 60 C
83. 0 _ es a PCR 6 530 560 610 640 670 705nm 4 SYBR Green I TaqMan HybProbe 4 0 20xL 100L
84. 7 3 HM PB1 NA 1968 1969 50 56 000 14 Z 000 nn HBNZ 1968 7 HZNZ H3 7 7 HZNZ H3 4 55 H H5N H7N7 H9NZ2
85. 1 44 4 3 3 e 4 C 29 e e e
86. DNA RT PCR PCR 2 0 Roche 2 PCR III A B 4b18 A B II I I 20 30 PCR DNA
87. 2 13 45 14 30 3 14 30 15 15 4 15 25 16 10 5 16 15 16 45 b 8 27 28 9 00 17 00 18 18303 6 1 8 27 ELISA 2 8 27 RT PCR 3 8 28 PCR 4 7 c AU TA 10590 16 00
88. 2 4 a 18 4 4 a 19 EE Sm gt 2 2 4 4 20 4 a 21
89. 4 33 2 S lt 30km 3 lt 30km 44 4 I AI AI 1 10 30 2 3 20 40 30 30 20 gt AI 2 2 AI
90. C 17 4c 18 A
91. gt 24 4 a 40 I 2 SE 4 SE SE 10 000 2 6 SE HACCP 2 IN FF 26 4a 41 1 2 v me 1 2 27 1 2 TOP 1 El le a be 4 A Ne 28 4 a 42 2 29 7 8
92. 4 b 1 4 b 28 4 b 3 2 1 4 b 4 4 b 5 4 b 6 4 b 7 2 RT PCER 4 b 8 4 b 17 3 PCR 4b 2 4 b 18 4 b 23 4 b 24 4 b 25 4 b 26 1
93. 3 2
94. 49 73 175 77 87 QO C8 GY 1 2 2 0 5mL x1 3 AIV 106 EID 4 9 ES AD 2 4 2 2 4 05 4 5 5 1 2 n log 10 0 1mL 1 1 Co 2 2F 1 3 11 4 C 34 8 A I VY
95. 5 AI 10 km AI 4 14 4 427 2 O 5 L wa 6
96. Al Al Al AI II 3 19 12 4a 47 1 AI 1 AI 2 Al Ey O 2 AI AI
97. 20 11 15 29 4 4 c 1 gt 4 c 60 1 4 c 3 2 4 c 4 4 c 24 3 4 c 25 4 c 35 4 4 c 364 c 55 4 c 56 4 c 57 4 c 58 1
98. 4 5 2 0 2 1 2 1
99. 48 48 N O OJ OO OJ 4b7 4 2 RT PCR 1 1 2 RT PCR RNA ELISA
100. 2008 11 29 I I 1 1
101. e e e GP 1 e 4 C 30 e e
102. pA 10km Al 0 S 0 0 4 5 AI 2 2 AI 1 0 AI 4 36 1 0 0 1 0 HF 8 F 9 AI
103. 8 1 2 3 4 a d b e c 4 20 Nb1IW A SNVNINa SSwh KU 8 27 28 ELISA RT PCR
104. Ds CE OE MD 4 C 51 9 00 1
105. 4 2009 2 7 15 30 15 45 PJ2 4 I
106. e e Pi 1 1 e e AGP 105 180 360 540 ELISA 270 450 650 2 0 1 1 2 9 1 2 A 1 Y 1 1 A RN 0 1 1 3 7 1 1 A TT TR 28 OO 0 CO 28 GY
107. 2 2 21 4 19 J PJ2 e mail Fax 4 20 4d21 PJ2 729 0292 084 936 2112 4671 064 936 2023 E mail shakai p 2 fTubac Tukuyama u ac jp PJ2 http www fukuyama u ac jp Tife pj2 422
108. 3 es PR eg Sr fA py i PL rt i i 30G 13 5 4 C 42 1 3 16 3 9 1 1 3
109. Al Al 4 50 Wy Y G gt K H A S lt mg HA NA 4 4 52 HA MA RNA 8 M H HA N NA HE N H 1 6 N 9
110. 9 30 10 30 2 10 40 11 10 4 C 52 11 20 11 50
111. 1 2 7 26 Cc 14 0 4c 15 1 60C 33
112. e e 1 AI 2 AI SAA hia 12 Avian Influenza Ai s es
113. gt ND e PCR e PCR AGP ELISA HD 6 Avian Influenza AI e e e 2 7 Avian Influenza AI e e 1 3 G o e
114. 4 d 1 4 d 22 1 4 d 3 4 d 6 2 4 4 d 7 4 d 8 4 d 9 4 d 13 4 d 14 4 d 18 3 2 1 4 d 19 4 4 d 20 4 2 PJ2 2 0 7
115. WHON WHO _ ea 4 4 4 58 20XX H5NI H5N1
116. 10km 5 30km 21 21 PCR 24 GP 24 24 4 c 10 1 1 2 gt 1 17
117. 4 C 43 2 3 2 32 3 5 _
118. H5N1 H5N1 38C 56 10 39 4 a 59 H5N1
119. 6 1 2 4 C 38 B 4 C 39 PJ2 4 GOO8 11 39
120. AI 1 2004 40 gt 60 kg 180 kg 1 6 lt 7 kg 6 2 lt 9 2 AI 2 4 4 4 32 2 2006 HSN1 70 AI 1 Al AI
121. XE CL 1 1 5ml Buffer AVL 554 1 RNA 5 6 u oO 2 140 1 15 3 15 25 10 4 5000rpm 5 5 5601 96 100 15 5000rpm 5 4 D 11 1 0 Ln 7 QIAamp Mini 2ml 5 630 4 1 6 000 xg 8 000rpm 1 QIAamp Mini 2ml QIAamp Mini 6
122. 1 3 RT PCR RNA RNA RCR PCR PCR DNA PCR
123. A 1 4 No 19 4 a 12 mm ICTVdb
124. H19 10 O O J O X PCR 4 C 3 2 2
125. 1 AI 2 AI PAI 100 PAI 9 PAI 4 4 28 AD AI 20 7 26 AI Al
126. 1 1 4b20 94C 94C 50C 72 4C 10 30 30 30 957902 2 10 12 13 14 15 16 17 18 19 4 D 21 gt VAN 1 ul 1 2 Ctr1 A1t Delete 3 OK 4 lLishtCyclet Software 4 0 master 5 New experiment Template AI 6 IMax Seek Pos 7 TM
127. 4 700 2 500 17 7 7 20 0 1 300 1 oppl 6 BE 20XX 4 700 2500 10 HONT 7 H5N 9 1 20XX 4
128. 700 2500 70 H2N XXr7 6 XX 4 44 67
129. Reverse transcript RT RNA DNA DNA DNA cDNA PCR RNA RT PCR cDNA 4 RNaseH RNA cDNA PCR DNA mRNA 3 RNA RTPCR 16 11 18 6227 NPD
130. A HTN1 H2N2 H3N2 H7N7 H3N8 HIN1 H3N2 H3N3 H4N6 H1 16 N1 9 H1 7 9 10 N1 2 4 7 HTN7 H4N5 HION4 g 4 53 1918 H1N1 1957 H2N2 1968 H3N2 0 1977 H1N1 _ XXXX H5N1 2 2 1918 1919 5 4 000 Z300
131. QIAamp Mini 5001 Buffer AW1 6 000Xg 8 000rpm 1 QIAamp Mini 2ml QIAamp Mini 500u1 Buffer AW2 20 000Xg 14 000rpm 3 QIAamp Mini 2ml 1 1 5ml QIAamp Mini QIAamp Mini Buffer AVE 601 1 6 000Xg 8 000rpm 1 RNA
132. e e 9 Avian Influenza AI e 1 e 2 ee et
133. gt 1 48 p9 4 15 1 FE Pu J EE 1 Ln WE Es em 2 2004
134. 1998 1999 H5N1 AI AI PAI AI PAI 2004 H5N1 AI 3 AI PAI PAI 2 3 PAI AI AI 4 26 Al H5N1
135. PCR 24 1 2 1 2 2 GP 24H 3 1 gt 5 10 10 2 5 30 40 ED 4 C 1 1 2 7 26 Con lt
136. 2008 11 28 2008 11 29 a 4c 41 gt a de eg nD 8 lt gt 16 2004 2 gt RE OE i i 2008 11 29 C0 11 gt 1 2
137. 1 HOAEL ne ober vid hir i Shest ed x100 x10 ET 10 AII Ea 100 3 ri i No 19 TE LJ Oe El Tt Wi te L Fe 2
138. 3 1 5 15 8 el 1 2 2 0 5mL x2 3 AIV 106 EID 4 3 4 A I V Te 3 A I V 1 4 1 2 5 5 ZA TG 2 si 1 1 2 n og 10 0 1mL 2 1 2 De
139. 4 C 12 NN J We 7 ET FE 2 Bsror
140. DY 3 1 2 O AI AE 3 AI 4 49 C4 J
141. 2 3 1 8 8 1 1 1 2 10 VO E 2 5 3 2 TW me 35 1 1 114 139 2 560 lt 10 lt 10 2 1 HI 20 2 1 1 7 2 1 3
142. 38 45 HIN 1918 3 10 99 i65 1919 7 6 1918 4 54 7 7 1957 1958 1 10 200 300 5 700 nn HZNZ 1957 4 7 6 Z
143. e e e 2 e 1 e e 0 1ppm e e e e 1 10 e 4 C 31 e e
144. gt Lr Po Eg Ee Lv 2 a 1 2 0 2 3 4
145. Sb _ Ra a I BSE BSE BSE BSE
146. 19 S80 19 5 13 45 14 30 14 30 15 15 15 25 16 10 16 15 16 45 44 4 B 8 27 28 10 00 16 30 16 ELISA RT PCR PCR C 10 11 2 16
147. 4 4 4 3 2 4 H5 H7 A 2004 2004
148. O 4 16 3 BSE 2001 9
149. 1 Avian InfluenzZa AI e 1925 79 15 1 12 3 5 2 14 14 28 22 5 3 5 1 5 16 6 26 12 25 39 615 19 1 13 1 2 23 5 4 29 1 2 30 9 1 e AI 1997 H5N1 AI 3 Avian InfluenzZa AI e LPA HPAT H5 H7 H5N1 H5N2
150. 2 1 cDNA 1 1 cDNA 1000 101 2 cDNA 10000 10 1 3 5 10 3 cDNA 100ng 1 2 cDNA 4 PCR 1 2 1 5ml Platinum SYBR Green qPCR SuperMix UDG Prim mix 10rM each 1 Da RK Ce CK en i le NP1200f CAG RTA CTG GGC HAT AAG RAC RA of GG A BE TT DE CE NPl529r GCA TIG TCT CCG AAG AAA TAA G 5 4 194 1 6 cDNA 1 1
151. yp Sh i he Pie Ey A ca 7 3 i gt 4 22 4 23 4 24 20 7 26 1 AI AI AI 1 AI 2 3 AI
152. 6 9 rs Fi leila eh ww 3
153. a ETa EE 1 5 REACH 2007 6 1t 44 9 B O 1 5 GXP ISO m GAP SO9000 a GMP a GHP HACCP mGDP GHP EN EE GCP mGPP a GGP No 19 No 19 4 a 10
154. N V Al AT 4 a 43 8h 7 0 kd 2 3 x 3 a gt gt N 1 Al 2 2
155. SYBR Green IT _ Multicolor HybProbe _ SimpleProbe TaqMan Probe i SO LED 2 0 Roche 4b23 6 4 4 Tel Fax e mail Tel Fax e mail Tel Fax e mail Tel Fax e mail 4b 24 084 936 2111 4047 084 936 2213 nakaura fubac fukuyama u ac jp 084 957 3414 084 957 3422 mi kaihatsu3 ikedatohka co jp 7
156. de Lv 1 A Pe ME io 1 2 3 0 0 4 5 2 1
157. PCR PCR 2 0 Roche 4 C 23 HACCP ssh Y Y Y Y Y go Y Y Y J EPE 9 3 4 15 4c 24 3 gt 2 0 1 1 2 9 4 C 25
158. 301 10 1 1 15 1 11 15 2 11 29 2 1 29 d 2009 2 7 13 30 16 00 903 4 8 a 7 26 13 30 lt 17 00 28 28101 26 b gt
159. 4 4 20 21 1 Nb1IW SVNIN 4 6 3 2O 12 5 99 68 26 18 29 3 70 101 5
160. PAT 4 AI AI 3 AI AI
161. RNA 260nm RNA OD260 1 40 4 g RNA ml 4 b 12 cDNA Invitrogen ThermoScript RT PCR System 0 5ml Random Hexamer 50ng 1 1 ul 65C 5 0 5 XSynthesis Buffer 5 XSynthesis Buffer 0 1M DTT RNaseOUT 40u 1 DEPC treated water ThermoScript RT 15units 1 25 10 50C 60 CE 1 1 RNaseH 37C 20 cDNA PCR PCR 4 b 13 PCR PCR 49 1 7 NN BA Ts lgTsTs ar 8 ie om Tea nA yz gz1 100 2
162. 224 4 5 1 Fres Marketof by fer_ i 21 14 63 14
163. 56 32 BD Flu 4 C 21 EusA OT aa oe RT Eb RT PCR ELISA A AGP a a Be 2 2 OO es 8 29 4 C 22 RT PCR RNA 2Z008 08 28 10 z0 m NA 35 RT PCR
164. DP ENT1 5 _ WHO 0 5 2 Z008 0 19 385 243 so 2 4 4 10
165. PCR 5 0 V 30 10 1 2 Ee DNA DNA DNA DNA PCR NP 329bp DNA RT PCR NP 329bp cDNA PCR RNA
166. 12 00 12 30 12 30 12 40 12 40 13 40 5 1 13 40 15 10 1 2 1 2 5 gt 9 EN 15 10
167. 20pmole ppL Primer mix Lee M et al J Virol Methods 97 13 22 2001 2 RNA RNA 3 RT PCR 1 1 19L O 2 RNA 1 zL RNA RN ase free 3 RT 4b16 4 4 PCR 1 49L PCR cDNA 1 L RN ase free 38 75L 10X Buffer 100mM Tris HCL pH8 3 500mM KC1 MgC1 5 00L dNTP mix 10mM 4 O04 L Ex Taq 5 w wL 0 25 Primer mix forward amp reverse 1 00 AL 5 PCR 94C uc oo 72C 6 PCR 329bp 4 D 17 5 3 PCR
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169. 49 A ie lt a ME a Ei mE n 2 38 L E
170. 65 95C 0 2 Ramp Ratel 8 Start runl 9 Samples Repl Of MD 0 Ne6 OF UE 11 Analysis Type Absorute Qantification Sample Type Standard Unknown Standard IConcentration 12 Analysis Absorute Quantification ITM cooling 4 bb 22 g 0
171. I 8 27 28 9 00 17 00 W471 18 18303 6 6 c 11 15 11 29 13 30 16 00 301 10 d 2009 2 7 13 30 16 00 903
172. 3 3 1 2 10 km 3 AI 2 AI 1980 AI 1980 H5N2
173. DRE NNN GN 3 APH VV RRM udp TSL MRO SVS NI AI RE RON ENT Ep RS GEN IK i BLLRMH STL TEE 3 O VO SG KK 8 RK DEMO kl jj NE SEE 843VSQG ESEPESASEEaEEEN SERIES ER 2 9 1 2 46ecU ENOGRSp 8 NHN PIE EA UE rls Ly JH K V HR DIY ERSNNNIEEGYSEVNN KEEO GRID G SA mm ER 3 G SH 9 SRD NU SS NIE HOG NY NL ff SEN NE 9 4 C 46 YNK 8 OS 0 ST 8 EQ NN OE AN 0 ne G JG 1 SKUIQG8 D 95 G 6 XR G 8 i ES 8 3340G
174. 4 a 70 4 a 71 42 1 BSE
175. 5 6 AS VI 3 1 2 p 3 G 3 7 1 1 lt 10 lt 10 485 113 ese 171 368 53 0 3 0 3 10 10 10 10 10 10 10 10 10 10 10 10 3 J 5 a lt 10 lt 10 lt 10 lt 10 lt 10 lt 1o Ne 0 8 0 8 0 10 0 10 0 10 0 10 1 H HI 20 1 5 8 15 9 2 1 2 4 C 33 7 Es oe A
176. 414 HIROSHIMA Frefectural Government 20 7 26 20 7 26 1 17 Eap ET lt 1 7 gt H5N2 6 1 4 1 578 4 a 16 2 19 4 4a17
177. Al AI AI 4 29 I AI 1 1998 1 1998 99 HSN1 2 1998 4 30 3 1998 1980 2 HSN1 1998 99 AI 4 31 AI
178. Avian influenza H5 7 KK ms GC EJ Ws H5N1 2 4 4 56 1918 H1N1 1957 H2N2 1968 H3N2 1997 H5N1 A HIN1 H3NZ 2003 10 Es 22 lt 2 7 a lies Nene ET rl f a Tm 14 ca IbN2 H5N1 HEN1 3E H5N1 j Ed A hh4
179. RE a 13 13 0 am 11 f TD 2002 12 3 HLit1 1 3 200H 11 29 200H 11 29 sd _ 2001 13 2001 13
180. TEL 084 963 4228 FAX 084 963 4265 TEL 084 936 2111 FAX 084 936 2023 E mail kikuta fubac fukuyama u ac jp 4 a 70 9 PT 4 e mail Fax 4471 PJ2 4 729 0292 084 936 2112 4671 064 936 2023 E mail shakai p 2 fTubac Tukuyama u ac jp PJ2 http www fukuyama u ac jp Tife pj2 44 72 2 PJ2 4 20 8 27 28 4
181. 0 IT GS1DSN9ORSG_6OS 80 BSS A RMN YDS J SO OP xihY G KIND ViG 19 AS 90 AANG pf 8 AS9 IQ QG XiIV7AG lt 4 G NGGPK 9SAQ7 nt 9 U8 IS G UK4 D dl WO IIIN 3kHIINWA WW ni RO Ne SQSNG JA 7 G 5 KK 9 V JA RQ JS Q 7 8 ORINKXS EC Nn A OAD W lt G46 9 GOO eine WRiQ C J
182. 1 5 a MS AI 5 AI 4 1 4 2 e ML e e
183. S 7 AI 4 4 45 2 30 AI OIE Al Al AI 2 3 AI 2 3
184. 00 400 x 1 11 lt NE TO 94 C io DNA 94 DNA 4C P 1 20XTAE 1ml 4 3 4 4b 14 1 XTAE 2 0 XTAE 2 0 PCR 1 1
185. 865 65 2566 0865 65 2566 0865 65 2566 0824 62 0881 084 936 2024 4 a 69 e mail kazutoshi_harada city 1bara okayama jp ohta bt fubt fukuyama U ac Jp nakaura fubac fukuyama u ac jp 2 A H gt NG H gt NG H gt NG H gt H gt NG H gt NG H gt NG H gt 8 TEL 082 513 3604 FAX 082 228 0396 TEL 084 963 4228 FAX 084 963 4265 TEL 072 254 9484 TEL 084 936 2111 FAX 084 936 2023 E mail kikuta fubac fukuyama u ac jp
186. AI 2 2 3 Fg 4 39 1 AU Se 23 AI 1999 gt gt 1999 gt AI CO gt 2003 gt AI Ai 2004 gt
187. ov LO Kyoto Prefecture All Rights Reserved 4 C 45 20 1 YDUSX ESGEa sa 8 SK DDS 0QGJS GS RE RP OSK SA NE A 8 81M E MNG GS CNKT HOO PQ EN S J DA MA G MA WVE 6 MiM LRC NAS SO SG ES GQ SIO NOIQSQAU ke NI et Gal i ENN OR 30 NK JS G NJ O PARISANNNEE SY G NE ES G WP IEA 9H 67 gt GR NN SGNT SV 36NDIO ek AS NCPWIIENIIEEEYYYM HG1 ve ee RNN NN CO SS NNN RS RS OR NN NNN ANNNSSRSSYNNSSNSNNSSKSNSSSSS RR NR NR NR SS SN NS SDKR MS 10 SG RT DC SGDG 7 2 ll 1111 FNKufSPuSPWIHHI BEEEGCN lt
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