CD3（IgG1）-FITC
Contents
1. 1 2 3 EDTA 6 4 90 52. T pan T CD7 CD2 CD5 CD3 CD3 CD4 CD8 T pan T pan B T B pan T CD3 T
3. 20 25 C CD3 lgG1 FITC 6604623 50 0 5mL 10 11 12 13 14 Schlossman SF 1990 Loss of suppressor T cells in active multiple sclerosis Analysis with monoclonal antibodies N Engl J Med 303 125 129 Bernard A Boumsell L Dausset J Milstein C and SchlossmanSF eds 1984 Leukocyte Typing New York Springer Verlag p 28 41 196 McMichael AJ ed 1987 Leukocyte _ Typing p 38 40 42 43 116 167 170 172 176 199 302 308 315 475 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T lymphocytes Cell 19 821 827 Aiuta F Cerottini J C Coombs RRA Cooper M Dickler HB Froland S Fudenberg HH Greaves MF Grey HM Kunkel HG Natvig J Dreudhomme J L Rabellino E Rittz RE Rowe DS Seligmann M Siegal FP Stjemsward J Terry WD and Wybran J 1975 Identification enumeration and isolation of B
4. 1 CD3 lgG1 CD3 lgG1 CD3 lgG1 3 EPICS CD3 lgG1 Level n Mean96 1S D 9 CV 1 10 13 8 0 4 3 0 2 10 57 8 0 9 1 5 3 10 92 1 0 3 0 3 2 6 3 10 CD3 lgG1 Mean 1S D 9 CV 1 EPICS 85 1 0 7 0 8 2 EPICS 83 9 0 3 0 3 3 EPICS 82 8 0 6 0 7 CD3 FITC EPICS n 51 Y 1 31 1 02X 0 984
5. T EBV CMV 19 65 CD3 lgG1 CD3 lgG1 EPICS S PLUS IV n Min Max Mean 1S D 30 54 84 72 2 6 2 mm 30 801 2962 1532 6 459 5 CD3 lgG1 UCHT1 CD3 CD3 lg9G1 B CD20
6. T Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3 T B T B T
7. 11 CD3 T CD2 CD5 T CD3 T T T T CD3 T CD3 1 10L 10 12 13 1 TQ Prep Multi Q Prep
8. 6 7 8 9 TQ Prep Multi Q Prep TQ Prep Multi Q Prep
9. lg E T T Smlg B lg Fc lg Smlg T B T B TdT HLA DR Smlg T BB T B
10. 20 25KD 2 T CD3 Ti CD3 TCR CD3 Ti TCR T CD3 lgG1 T T NK T 20 30 CD3 B CD3 CD3 ww UCHT1 CD3 lgG1 BALB c P3 NS1 1 AG4 1
11. 6604623 2009 11 2007 2 20600AMY00255000 877449 T CD3 IgG1 FITC 2 4 1 1 g T 20 000 CD3 pan T T
12. 3 10 x 10 mm 10 x 103 mm 3x10 mm Q Prep PBS 20 25 6 a gt 10 x 10 mm 10 20 x10 2 20 30 x103 3 30 40 x10 5 40 60 x10 6 60 100 x10 10 100 lt 200 x10 20 b 3 x 10 mm 1 20 25 C 500 x g 5 2 3 4
13. CD3 lgG1 3 4 No Wash No Wash TQ Prep Multi Q Prep TQ Prep Multi Q Prep
14. T T Common Variable Immunodeficiency DiGeorge T MS SLE B T T T T
15. lg lgG1 H L FITC Fluorescein isothyocyanate FITC Protein 2 5 468 509nm 504 lt 541nm 1 0 5mL BSA 0 29 0 01M Nacl 0 15M NaN 0 19 1 4 T T CD3 488nm FITC 4 FS 90 SS
16. FS Q Prep 0 5mL 3 EPICS 98 19 gt CD3 lgG1 FITC MslgG1 FITC CD3 lgG1 FITC MslgG1 FITC PBS 2 CD3 lgG1 Flow Count CD3 lgG1 STKS
17. LH 700 5 10x10 mm 1 100 L 1 Q Prep TQ Prep Multi Q Prep Coulter Immunology 3 A B C TQ Prep Multi Q Prep 1 12mm x75mm
18. 7546999 100 3 A B C 2 1 6603152 300 PBS 24mL 1mL 2 PBS PBS 6603369 1 500mL pH 7 2 0 2 3 MslgG1 FITC 6603846 50 0 5mL EDTA
19. 1 1 0 19 1 2 3 28 C
20. 135 0063 5 7 TOC Wa COULTER 6604623_0911
21. 10L 45 PBS 2 3mL 400 450 xg 5 PBS 25 1mL 30 2 250 kL PBS 2mL 10 400 450 x g 5 11 12 9 11 13 PBS 500 L 14 EPICS 1 2 Q Prep EPICS
22. 2 100L 3 10L 2 4 MSlgG1 FITC 10L 4 10 5 TQ Prep Multi Q Prep 6 EPICS 7 2 2 2 8 C 24 2 1 2 100L 10L MSlgG1 FTIC
23. CD3 lgG1 mm nm x 9 x 9 10 COULTER CYTO TROL 6604248 50 Fc Fc 1 296 T
24. vol 26 179 188 1986 1 vol 34 173 1987 FCM vol 19 Suppl 11 38 41 1987 vol 19 Suppl 11 42 49 1987 vol 19 Suppl 11 50 58 1985 vol 8 184 191 1987 CD vol 19 Suppl 11 5 7 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 4 4
25. Leeuwen E Capel PJA and Haanen C 1989 Auto logous transplantation of bone marrow purged in vitro anti CD7 WT 1 Ricin A Immunotoxin in T cell lymphoblastic leukemia and lymphoma Blood 74 1152 1158 Reinherz EL O Brien C Rosenthal P and Schlossman SF 1980 The cellular basis for viral induced immunodeficiency Analysis by moonoclonal anti bodies 125 1269 1274 Felsenstein D Camey WP Lacoviello VR and Hirsch MS 1985 Phenotypic properties of atypical lympho cytes in cytomegalovirus induced mononucleosis J Infect Dis 152 198 203 Rinaldo CR Ho M Hamoudi WH Gui X and DeBiasio RL 1983 Lymphocyte subsets and natural killer cell responses during cytomegalovirus mononucleo sis Infect Immun 40 472 477 Goldstein G Lifter J and Mittler R 1982 immuno regulatory changes in human disease defected by monoclonal antibodies to T lymphocytes In Monoclonal Anyibodies in Clinical Medicine McMichael AJ and Fabre JW eds New_York NY Academic Press p 39 70 Posner MR Reinherz EL Lane H Mauch P Hellman S and Schlossman SF 1983 Circulating lymphocyte populations in Hodgikin s disease after mantle and paraaortic irradiation Blood 61 705 708 Beverty PCL and Callard RE 1981 Distinctive functional characteristics of human T lymphocyte defined by E rosetting or a monoclonal anti T cells antibody Eur J Immunol 11 329 334 1986 T
26. and T lymphocytes from human peripheral blood Intemational Union of Immunological Scienties IUIS Report July 1974 Clin Immunol Immunopathol 3 584 597 Foon KA and Todd RF 1986 Immunologic classifica tion of leukemia and lymphoma Blood 68 1 31 Drexler HG Gignac SM and Minowada J 1988 Routine immunophenotyping of leukemias Blut 57 327 339 Reinherz EL Haynes BF Nadler LM and Bernstein ID 1986 Leukocyte Typing 1 York Springer Verlag Vo12 p 15 25 37 Caligiuri M Murray C Buchwald D Levine H Cheney P Peterson D Komaroff AL and Ritz J 1987 Pheno typic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome JImmnol 139 3306 3313 Reinherz EL Meuer S Fitzgerald KA Hussey RE Levine H and Schlossman SF 1982 Antigen recong nition by human T lymphocyies is linked to surface expression of the T3 molecular complex Cell 30 735 743 Meuer SC Acuto O Hussey RE Hodgdon JC Fitzgerald KA Schlossman SF and Reinherz EL 1983 Evidence for the T3 associated 90K heterodimer as the T cell antigen receptor Nature 303 808 81O Reinherz EL Cooper MD and Schlossman SF 1981 Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders J Clin invest 68 699 705 Oxford UniversityPress acute New Schmidt RE 1989 Monoclonal antibodies for diagnosis of immunodeficiencies Blut 59 200 206 Benjamini E and Leskowitz S 1991 Immnology A ShortCourse Second Edi
27. tion New York Wiley Liss p 211 244 Reinherz EL Weiner HL Hauser SL Cohen JA Disatso JA and 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Smolen JS Chused TM Leiserson WM Reeves JP Alling DW and Steinberg AD 1982 Heterogeneity of immunoregulatory T cell subsets in systemic lupus erythematosus Am J Med 727 783 790 Smolen JS Morimoto C Steinberg AD Wolf A Schlossman SF Steinberg RT Penne E Rein herz EL Rerchlin M and Chused TM 1985 Systemic lupus erythematosus delineation of subpopulations by clinicalserologic and T cell marker analysis Am J Med Sci 289 139 147 Morimoto C Reinherz EL Schlossman SF Shur PH Milis JA and Steinberg SD 1980 Alternations in immunoregulatory T cell subsets in active systemic lupus erythematosus J Clin invest 66 1171 1174 Morimoto C Reinherz EL Nadler LM Distaso JA Steinberg AD and Schlossman SF 1982 Comparison in T and B cell markers in patients with Sjogren s syndrome and systemic lupus erythematosus Clin Immunol Immunopathol 22 270 278 Pedrazzini AS Freedman AS Andersen J Heflin L Anderson K Takvorian R Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non Hodgikin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 2211 Preijers FWMB DeWitte T Wessels JMC DeGast GC Van
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