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ReverTra -Plus-TM

1. RT PCR RT G3PDH mRNA Primer F 559 gt 1 1237 1110 Intron Primer R Size bases 1634 90129 90 92 193 2 Control Primer F R Positive Control RNA RNA ELT Human G3PDH Glyceraldehyde 3 Phosphate Dehydrogenase in vitro 3 22 mer Poly A tail 3 G3PDH Housekeeping Gene TH mRNA Tumor promoting Phorbol Esters RNA hG3PDH RNA Poly A 22 mers AAAAAAAAAAAAAAAAAAAAAA TTTTTTTTTTTTTTTTTTTTTT Primer F Primer R Oligo dT 20
2. RT PCR RT PCR RNA cDNA cDNA PCR PCR DNA RNA cDNA ReverTra Ace High Fidelity PCR KOD Plus High Fidelity RT PCR ReverTra Plus 1 cDNA M MLV Molony Murine Leukemia Virus cDNA RNase H cDNA ReverTra Ace 14 kb cDNA 2 High Fidelity PCR
3. Control RNA 11 2 RNA DNA Negative Control RNA PCR DNase PCR Mg Mg 1mM 2 5 2 PCR RT PCR RT PCR 10 1 Takagi M Nishioka M Kakihara H Kitabayashi M Inoue H Kawakam
4. RT m Poly A tail RNA Oligo dT 20 PCR RT primer Mg Mg 2mM PCR 2 5 C 1min kb RNase Control RNA RNA E RNA RNase Inhibitor 1 ReverTra Ace 1 ul
5. 0 1 Diethylpyrocarbonate DEPC 37 C 12 121 30 8 RT PCR Total RNA tRNA mRNA rRNA RNA wet MagExtractor RNA Code No NPK 201F Total RNA 10 9 RNA PCR RT Random Primer RT 65C 5min 42
6. Hot Start PCR KOD Plus PCR buffer RT PCR 10 kb Patent pending HeLa total RNA 1 ug DNA Polymerase 6 8 kb 2 RT PCR RT PCR 1 RNA First strand cDNA Reverse Transcription RHT cDNA Second strand cDNA PCR JEF 5 Primer Reverse Transcription RT Synthesis of the First Strand cDNA ReverTra Ace Reverse Transcriptase cDNA 3 5 DNA Polymerase Synthesis of the Second Strand cDNA DNA Polymerase Amplification EL al 1 RT PCR 3 20 PCR 501 No 100 1 ReverTra Ace 100l 2 RNase Inhibitor 100 u 3 5 x RT Buffer 25 mM MgCb
7. tRNA 3 Random Primer Poly A tail Random Primer PCR Random Primer 30 C 10 7 RNA 1 RNase RT PCR RNase RNase RNA RNase 2
8. 400 ul 4 10 mM dNTPs 300 5 RNase Free H20 1200 u 6 Oligo dT 20 10 pmol 4 I 500 7 Random Primer 25 pmol 4 l 100 ul 8 Control Primer F 10 pmol u I 50 9 Control Primer R 10 pmol gl 50 10 Positive Control RNA 10 copies 4 l 504l 11 KOD Plus 100l 12 10 x PCR Buffer for ReverTra Plus 500 u 13 25 mM MgSO 500 Oligo dT 20 Random Primer dT zo 5 dN 3 Control Primer F G3PDH 5 ACCACAGTCCATGCCATCAC 3 20 mer Control Primer R G3PDH 5 TCCACCACCCTGTTGCTGTA 3 20 mer G3PDH Housekeeping Gene Control Primer F R 450b PCR Human Rat Mouse Swine Oligo dT 20 Random Primer p 9 cDNA DNA
9. C 2min 98 C 10sec OR 2 30 Tm 5 C 30 sec 68 C 1 min kb 25 40 4 C Hold 1 Tm 72 C PCR 2 2 94 C 2 min 94C 2 min 98 C 10 sec 25 40 98 C 10 sec 68 C 1 min ieee 74 C 1 min kb 5 4C Hold 98 C 10sec 72C 1 min Pane 5 98C 10sec 70C 1 min 5 98 C 10sec 68 C 1min ae 25 68 C 7 min 4 C Hold Control Primer 60 6 1 Control Primer R RNA 2 Oligo dT 20 Poly A tail mRNA RNA rRNA
10. dNTPs 2ul RNase Inhibitor 1ul ReverTra Ace 1l Total Volume 20 ul 30 C 10 42 C 60 85 C 5 40C Hold Random Primer 30 10 RNA Positive Control RNA Control Primer R cDNA 85 5 PCR ReverTra Ace 1 lt 2 PCR PCR PCR 35 10 x PCR Buffer 5 25 mM MgSO 3 ul 10 mM dNTPs 1 ul 10 pmol 11 1 5l 10 pmol ul 1 5l KOD Plus dul 1 cDNA 2 Total Volume 50 1 94
11. 3 RNA 4 RT PCR 1 DNA PCR 2 UV PCR 5 1 PCR RNA RNase Free HzO Xul Primer Oligo dT 20 10 pmol 4 1 5 l Random Primer 25 pmol 4 l 1 wl 10 pmol p11 1 wl 1 RNA Total RNA Tug mRNA 10 100 ng Yl Positive Control RNA 10 copies 1 41 Total Volume 12 65 C 5 RNA 12l 5x RT Buffer 4 l 10 mM
12. Extractor RNA Total RNA none NEON Magical Trapper MS 100 bp DNA 100 DNA 035 200 bp DNA 100 DNA 031 1 kb DNA 300 DNA 032 14 RS Hi 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
13. Ideas amp Chemistry High Fidelity RT PCR kit ReverTra Plus Code No PCR 501 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3297K RNA RT PCR 1 cDNA
14. i B Oka M and Imanaka T Appl Environ Microbiol 63 4504 4510 1997 2 Hashimoto H Matsumoto T Nishioka M Yuasa T Takeuchi S Inoue T Fujiwara S Takagi M Imanaka T and Kai Y J Biochem Tokyo 125 983 986 1999 3 Mizuguchi H Nakatsuji M Fujiwara S Takagi M and Imanaka T J Biochem Tokyo 126 762 768 1999 4 Nishioka M Mizuguchi H Fujiwara S Komatsubara S Kitabayashi M Uemura H Takagi M and Imanaka T J Biotechnol 88 141 149 2001 5 Hashimoto H Nishioka M Fujiwara S Takagi M Imanaka T Inoue T and Kai Y J Mol Biol 306 469 77 2001 6 Imanaka T and Takagi M J Clin Inst Chem Engrs 32 277 288 2001 12 11 Code No ReverTra Ace a RT PCR cDNA mee aoe ReverTra Ace 10 000 Ux1 TRT 101 10 000 Ux1 x5 TRT 101X5 10 000 Ux1 x10 TRT 101X10 KOD Plus 200Ux1 KOD 201 200Ux 1 A x5 KOD 201X5 200U x 1 AX x 10 KOD 201X10 KOD Plus Ver 2 200U x1 KOD 211 200U x 1 A x5 KOD 211X5 KOD Plus Neo 200U x1 KOD 401 200U x 1 x 5 KOD 401X5 200U x 1 x 10 KOD 401X10 RNase Inhibitor recombinant 2 500U x 1 SIN 201 2 500U x 1 x5 SIN 201X5 10 mM dNTPs 2umol NTP 301 Oligo dT 20 1 nmol FSK 201 Random Primer 2 5 nmol FSK 301 Mag

ReverTra -Plus-TM

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