RNA-direct TM SYBR ® Green Realtime PCR Master Mix
Contents
1. PCR Rs 14 7 One step Realtime PCR One step Realtime PCR RNA direct Realtime PCR Master Mix 500ul x 5 QRT 101 500ulx5 x5 QRT 101x5 RNA Total RNA MagExtractor RNA 100 NPK 201F Magical Trapper 1 MGS 101 PCR PCR 1mlx5 QPK 101 Realtime PCR Master Mix 1mlX5 X5 MI 1 ROSA2. GC RNase RNA RNA RNA DNA DNA DNase DNA
3. 5 200bp 15 data collection 10 Detector F1 3 PCR 2 PCR data collection Temperature Transition Rate 20 C sec 2 C sec 11 5 Total RNA
4. DNA RNase free RNase free DEPC DEPC DEPC PCR RNase free Total RNA Total RNA AGPC Acid Guanidium Phenol Chloroform Total RNA DNA DNA DNase
5. 0 2ul PCR 10ul 2 Applied Biosystems 7900HT SYBR Green 1 Distilled Water KNase free grade up to 50 HI RNA direct SYBR Green Realtime PCR Master Mix 25 HI 50mM Mn OAc 2 5 ul 2 5mM Sense Primer 10 pmol 0 2uM Antisense Primer 10 pmol 0 2uM RNA sample lt 2 5ug Total volume 50 Hl Distilled Water RNase free RNase free lt RNase free DEPC DEPC DEPC PCR RNase free
6. RNA PCR Tm 55 65 C Detector Reporter SYBR Quencher None Flurorescent Passive Reference ROX PCR data collection 30 3 PCR 2 PCR data collection Applied Biosystems 7900HT Standard Fast SYBR Green
7. 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo Jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
8. PCR 3 RN4 aoirecty SYBR Green Realtime PCR Master Mix DNA RNA 4 MA g7ecf SYBR Green Realtime PCR Master Mix LineGene BioFlux LightCycler Roche Diagnostics passive reference Applied Biosystems Realtime PCR PCR 2 QRT 201 50pl QRT 201X5 x 100 20 C RNA direct SYBR Green 4 C UE
9. MA g77ec7 SYBR Green Realtime PCR Master Mix SYBR Green PCR 1 PCR RNA direct SYBR Green Realtime PCR Master Mix RNA PCR 2 RNA GC rich RNA direct SYBR Green Realtime PCR Master Mix PCR rTth DNA Polymerase RNA r7th DNA Polymerase GC rich
10. Distilled Water 2 6 1 twt lFTUJ SYBR Green data collection SYBR Green data collection PCR PCR
11. DEPC DEPC PCR RNase free 2 6 pmol 0 2 0 6uM Mn OAc 2 2 5mM RNA Mn OAc RNA Total RNA 500ng poly A RNA mRNA 100ng 2 PCR RT PCR
12. 1mlx5 QPK 201 SYBR Green 1mlx5 x5 QPK 201X5 SYBR Green Realtime PCR Master Mix 1 SYBR Green SYBR Green Realtime PCR Master Mix Plus 1mlx5 x5 9 1mlx 1 QPS 101T PCR 1 67mlx 3 QPS 101 THUNDERBIRD Probe qPCR Mix 1 67mlx 3 x5 QPS 101X5 1mlx 1 QPS 201T 1 67ml x 3 QPS 201 1 67mlx 3 x 5 QPS 201X5 PCR cDNA PCR cDNA ReverTra Ace9 qPCR RT Master Mix DNA PCR cDNA ReverTra Ace qPCR RT Master Mix with gDNA Remover ReverTra Ace SYBRY Green PCR THUNDERBIRD SYBR qPCR Mix 200 FSQ 301 10 000Ux 1 TRT 101 10 000Ux 1 x5 TRT 101X5 10 000U x 1 x 10 RT 101X10 DNA RNase 2 500U x 1 SIN 201 RNase Inhibitor Recombinant 2 500U x 1 x 5 SIN 201X5 15 HPCR
13. 13 PCR PCR PCR GC PCR 3 we PCR
14. RNA 20 C 3 PCR LineGene BioFlux Applied Biosystems Realtime PCR LightCycler Roche Diagnostics PCR 20 30mer GC 40 60 200bp
15. Mn OAc gt 2 5mM RNA Mn OAc RNA Total RNA 1pg poly A RNA mRNA 200ng 10 2 PCR RT PCR Denature 1 90 C 0 30 61 C 20 00 2 95 C 0 30 06 Cycle 95 C 0 05 45 Cycles 55 C 0 10 74 C 0 15 data collection Melting Curve Analysis p 2 Denature 1 90 C 30 RNA PCR Tm 55 65 C
16. 10 30 pmol 0 2 0 6uM Mn OAc gt 2 5mM RNA Mn OAc RNA Total RNA 2 5ng poly A RNA mRNA 500ng 2 PCR RT PCR Denature 1 90 C 0 30 61 C 20 00 2 95 C 1 00 06 Cycle 95 C 0 15 45 Cycles 55 C 0 15 74 C 0 45 data collection Melting Curve Analysis p 2 Denature 1 90 C 30
17. DNA Total RNA mRNA 1 2 poly A RNA mRNA poly A RNA oligo dT polyA mRNA mRNA mRNA Total RNA RNase 4 1 LineGene BioFlux SYBR Green 1 Distilled Water KNase free 9rade up to 10 HI RNA direct SYBR Green Realtime PCR Master Mix 5 HI 50mM Mn OAc gt 0 5 HI 2 5mM Sense Primer 2 pmol 0 2uM Antisense Primer 2 pmol 0 2uM RNA sample lt 500ng Total volume 10 HI Distilled Water RNase free RNase free RNase free DEPC
18. DNase AGPC Acid Guanidium Phenol Chloroform Total RNA DNA DNA 1 Distilled Water RNase free 9rade up to 10 HI Total RNA X Hg 10x DNase Buffer 1 HI RNase free DNase 10U ul 0 5 Hl Total volume 10 Hl 2 10 30 100ul Distilled Water 100ul TE 5 12 a 5 500m 5 5ul 20mg ml 100ul 5M 200ul 20 C 30 a 5 70 6 5
19. Denature 1 90 C 0 30 61 C 20 00 2 95 C 0 30 06 Cycle 95 C 0 15 45 Cycles 55 C 0 15 74 C 0 30 data collection Melting Curve Analysis p 2 Denature 1 90 C 30 RNA PCR Tm 55 65 C PCR Channel F1 3 PCR 2 PCR data collection BioFlux LineGene 33
20. 3 LightCycler Roche Diagnostics SYBR Green 1 Distilled Water KNase free grade up to 20 HI RNA direct SYBR Green Realtime PCR Master Mix 10 HI 50mM Mn OAc 1 HI 2 5mM Sense Primer 4 pmol 0 2uM Antisense Primer 4 pmol 0 2uM RNA sample lt 1Hg Total volume 20 Hl Distilled Water RNase free RNase free lt RNase free DEPC DEPC DEPC PCR RNase free 4 12 pmol 0 2 0 6uM
21. Realtime PCR Master Mix 2 Mi QRT 201 x 5 50mM Mn OAc gt 20 C 500ul x 1 RNA direct SYBR Green Realtime PCR Master Mix dNTPs r7 DNA DNA PCR passive reference SYBR Green 2x RNA Mn OAc RNase free 1x 20 C 20 C 10 2 4 C 50mM Mn OAc 1 20 volume 2 5mM
22. TOYOBO ldeas amp Chemistry One step qPCR Kit RNA direct SYBR Green Realtime PCR Master Mix Code No QRT 201 QRT 201X5 TOYOBO CO LTD Life Science Department OSAKA JAPAN AS3587K 1 2 3 5 6 7 eSGEaisdsas 4 2 C7 NOTICE TO PURCHASER LIMITED LICENSE Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 079 352 5 789 224 5 618 7 11 6 127 155 5 407 800 5 322 770 5 310 652 and claims outside the US corresponding to US Patent No 4 889 818 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent such as the patented 5 Nuclease Proc
23. ess claims in US Patents Nos 5 210 015 and 5 487 972 and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use Only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 LincolIn Centre Drive Foster City California 94404 USA LightCycler Idaho Technology Inc Roche Molecular Systems Inc SYBR Molecular Probes Inc 1 RNA direct SYBR Green Realtime PCR Master Mix Thermus thermophilus HB8 DNA rTth DNA Polymerase 2 Mn 1 PCR 2x PCR 1
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