次のリンクは新しいウィンドウを開きます取扱説明書
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1. Gica BgEB4UARRERXVARRD semen me T103 0022 sm ABS BAISSHIZTBH2381015 03 6214 1090 T541 0048 Att XE REBI2T E5815 T812 0007 ER rbIS P RIEZS2T H22 83S ZHDNAT EH EIU LE U 7E EI H ugiuuu t O O 20 uL 10x Reaction buffer 1 0 uL 100 uM 6R5S primer 20 uL ULL 2 0 uL total 10 0 uL X 6R5S prine rN rN rN rN rN rN 6mer NO RNA mx B DI D HH HL U BO V B 7E BI BLUE U 2 95 d 100000030 gr 01 0 80 O 300000000 00400 0 10x Reaction buffer 25 mM dNTPs 100 mM DTT 1 0 uL Pyrophosphatase 200 U mL 0 1 uL o o 5 1 uL phi29 DNA DODO O 0 05 ny nl 20uL Total 10 0 uL 4 30 Q 16 65 q 100 d OU U DO phi29 DNA 1 000 0 5000000 uult 1 0 uL 0 8 uL OSLOON L O m phi29 DNA 4000000000 Nor template T 0 O 000 udgaduuulu uut 0010 DO UbanH EcoRl TH L L MERGA A EE e O JJpUCI8 DNA0 O B HH BLU 000000 ug 27kopr n 0000000 00 00 pucis 3C 3k M 10 10 10 10 pUcis at M 10 10 10 10 Non template Non template kbp lt pUCi8 2 7 kbp B N URB MEA n AE DU OO 0000000000000 http www kanto co jp e mail reag infogms kanto co jp 06 6231 1672 092 414 9361 17447 1 Fk26F10 fFRk 200000000 1 DNA 0O D DIC II gi pagg it UL 2 p00000 000 800000000000 000000 ulli B B BE UOT C D UO DEBE D EE EE D BBNA 000000000 00 00000 DNA freer O O 1 El E E L3 z Tu 100000000 psonmm BO 0000000000000 KOACH T 50 FOOOOooo0d00002. 00 mon DT TTE LLHH HLELE EIE o DEO BE EE UI LI E H E O coder 10752 63 Pyrophosphatase JP yrophosphatase inorganic Roche code 110108987001 JeR5S primer IHPLC O LI A A ad D EO OD CDL CI 27 x e MEN T103 0022 RRA IX HZK SZEBT2T H23815 03 6214 1090 BREA A IED ERA EAN 541 0048 XD AERBHI2TEB5 18 06 6231 1672 7812 0007 tEMTM SERHZD2T822838 092 414 9361 A t 110000 1 Blanco L and Salas M 1984 P VAS 81 5325 5329 2 Blanco L et al 1989 Biol Chem 264 8935 8940 3 Garmendia C et al 1992 Biol Chem 267 2594 2599 4 Esteban J A et al 1993 Biol Chem 268 2719 2726 b Takahashi H et al 2014 PLOS ONE 9 e82624 6 Lizardi PM et al 1998 Mat Genet 19 225 232 7 Dean FB et al 2002 PNAS 99 5261 5266 8 Takahashi H et al 2009 BioTechniques 47 609 615 http www kanto co jp e mail reag infogms kanto co jp
3. 17447 1 Fk26F10 fFRk 110 00 O phi29 DNA ODODO O YU Bacillus subtilis O phi29 D ug DNAD OOOO CO 0000 0000030 q O O DO0000d8do0 00000000000 0000 2 00 DApopoa00 0 DNAE HB EO LO BELICE I TH e3os5 uuluuututttututtu ugbNAT 0000 JHHHBHBdguggge OO 0O DNA T270 kopo O 9000000 0 Ean e E O D B BLUE DE D LU OOOO DNA ET D D LL DU O L 200 utt 000 phi29 DNA Y 4000 D O 00 phi29 DNA polyrrerase set OUUU 10195 96 phi29 DNA OOOO O 25 Lx 4 LL E BT LH 10x Reaction buffer 100 uLx 41 DODoOO 1l 25 20 C JODE phi29 DNA 1 OO0000000 300 D O O phi29 DNA 0800 O 33 mM Tris HCO pH 7 5 100 mM 0000000 O1mM EDTA 1mM DTT 1mM THP 0 5 96 Tween20 0 5 NP 40 50 9e 000000 0 05 my mL phi29 DNA ODO O 10x Reaction buffer 350 mM Tris HCI pH 7 6 500 mM 1H E EL O 140 mM O HB DLB D H1 1300 mM 00000000 400 0 00 O O 00 Rolling circle amplification REAL Multiple displacement amplification MDAUI Multiply primed RCA with modified RNA primer Cell free cloning 300 E O O LU ULBOLDLD DLL L uuguauululiuiuiui RNese O 0O ul 0000000 01190000 DNA L 9 000 ng hr 25 O0 DBNAL 1 HL BHL E HH BLU DE HIELO D 00 DNA ET E ei maamuuuututu 1x Reaction buffe 50 ug nL M13 ssDNA 10 uM M13 primer 1mM dNTPs 5mM DITTO 1U mL Pyrophosphatase 5 ug mL phni29 DNA 0000 O Modo 30 dj M13 prime 5 GTTTTCCCAGTCACGAC 3 J17mer
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