ファイトプラズマ検出キット - 検査・診断試薬（非医薬品）
Contents
1. 1 LAMP DNA Phytoplasma2. Phytoplasma Phytoplasma LAMP Phytopla
3. 2 x 5 mm DNA Phytoplasma 2 95 C 10 1f a DNA DNA
4. 1967 700
5. A 240 260 nm 350 370 nm UV 320 nm A B 15 6 1 J 1996 phytoplasma 62 5 53 Lawson RH Smith FF 1980 Three stable types of Hydrangea virescence that differ in severity in foliage and flowers Plant Dis 64 7 659 2010
6. http www_ nippongenematerial com 2 24 NE0111 0 20 C 20C 30 Phytoplasma 5000u 20C 2 Ca P japonicum Ca PP asteris 24 es 24 24
7. http papilio ab a u tokyo ac jp cps hospital 1 1 1 TEL 03 5841 0567 E mall byoin todaiagriJD 14 5 A Phytoplasma Phytoplasma Phytoplasma B
8. MSDS 3 61C 0 5 10 ul 10 100 ul 200 1 000 ul 63 C 80 C 95C 1 5 ml 2 0 ml _ 0 2 ml lt ea UV 240 260 nm 350 370 nm
9. 70 iv 1 v DNA Phytoplasma DNA CTAB DNA DNA 5 ng ul Phytoplasma 10 100 0 ul
10. 1 x 3 61C 63C 60 P asteris 61 C P japonicum 63C 1 2 60 80 C 2 1 UV UV 240 260 nm
11. LAMP DNA DNA P asteris P japonicum Phytoplasma 61 C 63C 60 LAMP DNA DNA DNA DNA DNA 18
12. MSDS http nippongene analysis com 8 9 LAMP LAMP 10 e Genome Order e Genome Order http genome e mp jp 5 1
13. gt 1 I II 13 gt P asteris P japonicum IV
14. 1 C EDTA D A A
15. 2 LAMP Loop mediated Isothermal Amplification LAMP DNA DNA LAMP Eiken GENOME SITE http loopamp eiken cojp amp amp ISO 13485 2003
16. LAMP Loop mediated Isothermal Amplification 2 2 3 ENA A ARAMA ENO CARON RA NDNA AN IE EA 3 4 i EN fd 5 5 CN NINNNWNMNMNMNNWINNNWWWMNWNWWouunne 7 FE 9 DNA 5 15 6 IE 16 7 17 Phytoplasma 1 LAMP Candidatus Phytoplasma asteris Candidatus Phytoplasma japonicum LAMP DNA
17. 20 C 6 20 C Phytoplasma Phytoplasma DNA DNA 1
18. DNA DNA Phytoplasma DNA DNA CTAB DNA DNA 5 ng ul
19. 1 1 DNA DNA 2 0 ul 20 0 ul CDNA 2 0 ul Phytoplasma 2 0 ul Phytoplasma
20. 1 5 ml DNA NE0051 4 7 1 Phytoplasma 1 100 0 u Phytoplasma 100 0 ul 2 Phytoplasma 3 95 C 10 DNA 4 P asteris 189 0 ul P japonicum a _ Phytoplasme
21. 2008 Namba S Kato S Iwanami S Oyaizu H Shiozawa H 1993 Detection and differentiation of plant pathogenic mycoplasmalike organisms using polymerase chain reaction Phytopathology 83 786 2004 Notomi T Okayama H Masubuchi H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA uc e c Ac gs Res 28 12 e63 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechnigues 12 3 358 Sawayanagi Horikoshi N Kanehira T Shinohara M Bertaccini A Cousin MT Hiruki C Namba S 1999 Candidatus phytoplasma japonicum a new phytoplasma taxon associated with Hydrangea phyllody Int J Sysr Bacteriol 49 3 1275 16 7 Phytoplasma 2 0 ul 25 0 ul 1 DNA 61 C 60 Phytoplasma Phytoplasma 2 0 bl 1x 10 1x 10 Phytoplasma P
22. Phytoplasma Phytoplasma 1 100 0 pl 20 C 2 P asteris P japonicum Phytoplasma Phytoplasma 20 C 1 x 3 10 1 5 ml 2 0 ml
23. 1 x 3 P mr 21 0 ul 189 0 ul 525 0 ul P japonicum 2070 ul 575 0 TI 1 Phytoplasma 23 0 ul
24. 4 5 6 Phytoplasma 7
25. 10ul 90ul 1 5 1 23 0 pl 6 2 0 ul Ee DD EE Phytoplasma wz DNA Phytoplasma 2 0 ul 1 2 0 ul 1 J J J 2 0 HI 7 yw 20 0 ul 6 61C P asteris 63 C P japonicum 60 7 80C 2 8 UV 7 L 0 2 6 Phytoplasma Phytoplasma Phytoplasma
26. DNA 18 19 20 2012 11 NIPPON GENE TEL 076 451 6548 TEL 03 5977 5573 FAX 076 451 6547 FAX 03 597 5574 E mail support nippongene analysis com E mail feast egenome csjp fujitsu com URL http nippongene analysis com URL h
27. Phytoplasnna Detection Kit version 1 0 0 ig NE0111 DO version 1 0 0 1 LAMP Candidatus Phytoplasma asteris Candidatus Phytoplasma japonicum 2 DNA 3 2 6
28. 350 370 nm 320 nm UV 0 UV 60 UV
29. asteris P japonicum 1 7 Candidatus Phytoplasma asteris DNA _ a BV Candidatus Phytoplasma japonicum
30. sma 1 1 lt gt i ii 100000 ppm 1 ii
31. ttp genome e mpJp Copyright 2012 NIPPON GENE CO LTD All Rights Reserved
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