Contents
1. Peperomia 1600 Da 22. OD Peperomia 1 epe7o7z7 boninsimensis I Figure 1 1 3 3 Peperomia japonica 2
3. DNA 3 1 DNA
4. DNA 2 3 TLC NMR MS HP
5. 42 Ohi Toma et al 2010 HEPES CTAB 3 Wanke et al 2006 Samain et al 2009 DNA ITS DNA 7 z intron matK PCR ITS
6. 34 17 FJ424471 pinoi 56 FJ424467 mandonii DQ21271 6gracillima FJ424470yestta linde 4 2 DQ212744 macrostachya 8 4 DQ212743 vinasiana 3 11 oba 07 58 3 06 scandens 07 53 14 lorgispicata 07 61 26 DQ212742 fagerlindi 12 uocarpa 07 59 FJ424468 polystachya 3_ DQ212763 blanda PE02 puberulispica 35 pubeulispica 5 151 31 sp Hawaii07 78 54 sp Hainan 1 PE01 camptotricha 3 1 19 verticillata 07 66 25 sp 07 72 36 camptotricha 5 152 09 rubela 07 56 4 5 22 sp07 69 bp 24 rotundifolia pilosior07 71 DQ212762 pitcairnensis 2 poQz12760 sp 32 sp 07 83 40japonica Okinawa 5 r DQ212761 bicolor 2 02 bicolr07 49 5 DQ212757glabella 13 144 sp 07 107 1 Sh 7 38 sp Honalulas DQ212756 tuisana 61 3 2 1 26 sp 07 73 3 DQ212754 rotundifolia 3_ 03 rotundifolia 07 50 6 DQ212752 magnoliifolia 8 7 08 itymaloides 07 55 DQ212750 lanceolatopeltata 5 8__ FJ424466 lehmannii 1 3 DQ212725 marmorata PE03 caperata 7 17 oriseoargentea 07 64 42 15 sp 07 62 18 manmorata 07 65 8_ DOQ212737 sdiri DQ212740 metallica 7 DQ212739 repilis 1 DQ212735 maypurensis 6 23 sp 07 70 9 DQ212734 argyreia 41 sp 10 2 FJ424469 nivalls 10 3 eolumella 07 85 37 fereyarae 9 8__ DQ212721 dolabriformis 9 DQ212719 fraseri 13 fraseri 07 60 12 01 sp 07 48 10 rifolia 07 57 04 quadrangularis Z20 puteolata 30 puteolata 07 77 16 d
7. For the bases of development of plant resources in the genus Peperomia Piperaceae an easy tO use identificatton System was tried to construct Morphological database was prepared but still not completed due to unsatisfied condition of the material cultivated Several chemicals including secolignan that are potential tool for the identfication of taxa were recognized And a molecular phylogenetic tree with 69 OTUs including 29 known sequences was constructed Integration of the obtained information is on the way 3400000 1020000 24420000 Piperaceae epero 1 Piperaceae 8 12 1400 3000
8. 2 Peperomia glabella Peperomia glabella Figure 1 4 7 Ra gt 9 OR1 HaCO 17 R RH pa HaC 3Ho gt oH 14 R Clls Ra Ul lignar secolignan Tieure 2 Figure 1 1 7 secohgna figure 2 seco Pipery Figure 1 Peperomia Piper 4 P Peperomia 3 Peperoima obtusifolia Peperomi
9. RE ty DNA 2007 11 16 12 6 Shivaji University Botanical Surveu of Imdia Shivaji University Peperomia 2008 11 16 11 23 2009 2 6 9 ee Peperomia 2009 8 20 23 2010 1 27 29
10. 7 intron matK 7 intron matK 2500bp Johnson and Soltis 1994 Ooi et al 1995 Wanke et al 20000 3 PCR tnK intron 3 matK 3 PCR matK trnK intron 5 1500bp 3 tt 2 r nKintron 40 dGenbank Wanke et al 2006 Samain et al 2009 DNA 100bp 29 69 Samain et al 2009 1
11. C 19 22 5 1 C es 2007 2009 19570096 JR Construction of an identification system in considerins molecular ny and phytochemistry MURATA HIROKO 20167620 DNA 3 29 69
12. LC 4 DNA DNA DNA Smm X 3cm Setoguchi and Ohba 1995 HEPES 2 4 2 Hydroxyethy1D 1 piperaziny1 ethanesulfonic acid CTAB cetyltrimethylammonium bromide DNA PCR DNA trnL trnL 7 rDNA ITS PCR PCR GeneClean II Kit BIO101 USA DNA DNA ABI PRISM Big Dye Terminator v3 1 Applied Biosys
13. a obtusifolia figure 3 8 14 13 17 8 14 Peperomia galioides Piper epe7ozzg Peperomia 15 17 C C Peperomia Piper
14. ahlstedi 07 63 07 pereskifolia 07 54 DQ212715 sp 2 DQ212714 cocatum 5 changes Rig 4 DNA Rig 4 5 6 1 MURATA HIROKO 20167620 2 INATOMI YUKA 00258089 MURATA JIN 90134432 TANAKA NORIYUKT 6013706 RAL 3 A A WE WA gp AA AL Li 1
15. tems USA AutoAssembler Applied Biosystems USA Clustal X Thompson J D et al 1997 PAUP ver 4 0 beta10 for Mac Swofford 2002 1 000 4 1 Peperomia A di te
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