取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. Endotoxin 8 1 3 4 230 232 2006 2 Li X Zhao X Fang Y Jiang X Duong T Fan C Huang C C Kain S R J Biol Chem 273 34970 34975 1998 3 Andrew G B Joanna B Cameron S O and Peter N C Plasmid 44 173 182 2000 13 9 Code No High Fidelity PCR KOD 201 KOD Plus TEM PED High Fidelity PCR T KOD Plus Ver 2 G KOEN E y EI ij Taq A 02 PCR 250 U BTQ 101 Blend Taq JI KF sE in Blend Taq Plus 50 LGK 101 Ligation high DNA 903 Competent high DH5 a DD PCR PIK 151 InsertCheck Ready InsertCheck2. 1 Emerald Luc Emerald Luc 1 Emerald Luc D luciferin
3. 2 in vitro Emerald Luc Luciferase Assay Reagent Code No ELA 101 10 7 5 1 pELuc test pELuc test Multiple cloning region Emerald Luc gene ELuc SV40 late poly A signal B lactamase Amp r gene background reduction signal 2 pELuc PEST test pELuc PEST test Multiple cloning region Emerald Luc gene ELuc PEST SV40 late poly A signal B lactamase Amp r gene background reduction signal GE pELuc PEST test Hind IIl Pst 1 Neo l 1 89 91 1719 1733 1958 2052 2912 3743 4222 1 70 91 1716 1717 1839 1863 2087 2182 3042 3873 4352 Kpn 1 Poly A Gal TE i amp Poly A 3743 ori c Mlul 28 pELuc test ELuc 4222bp Emerald Xba 11720 J Sca l 2356 Not 11726 Sspl 2032 Sac 1959 BamH I 1977 Sac II 1965 Sma 1971 Poly A
4. Code No Emerald Luc Lysis Solution 100ml ELA 201 2 Emerald Luc Code No Emerald Luc pELuc test 10ug ELV 101 Emerald Luc Short life PELuc PEST test 10ug ELV 201 3 Emerald Luc 1 Emerald Luc Pyrearinus termitilluminans D luciferin 2 3 4 5 HO COOH ELuc OY ATP O gt Oxyluciferin AMP PPi CO Mg D luciferin 2 Emerald Luc fLuc 3
5. 9 pELuc test i EEn 5 CAATGT ATCTTATCAT GTCTGGAT GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAGGT_CGACGGTATC Xhol Sall Clal Ban lll GATAAGCTTA CGCGTGCTAG CGCATGCAGA TCTACTAGTC GGCCGGATAT CGAATTCCTG CAGCCCACCA CCATGGAGAG Hind IMI Miul Nhel Sphl Bgll Spel EcoR V EcoR Pst Ncol L gt ELuc AGAGAAGAAC GTGGTGTACG GCCCCGAGCC CAAGCACCCT CTGGGCAACT TCACCGCCGG CCACATGC CGGGGCTCGG GTTCGTG 5 1 pELuc PEST test 2 SV40 poly A
6. Emerald Luc in vitro 7 jin vitro 1 ELuc ELuc PEST PT e ui c n ro in vivo yz D Iuciferin g Tg W 1 SRI LHO BHS NBK
7. 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order _lifescience toyobo jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
8. 2 Short life Emerald Luc O C PEST 6 7 8 ELuc 6 Emerald Luc Short ELuc EE life Emerald Luc PEST PEST RI 7 Emerald Luc Short life 3 0E 05 2 5E 05 Emerald Luc Eluc Emerald Luc Short life EN ELuc PEST CHO Na gi 1 5E 05 95058 1 0E 05 ELuc 4 ELuc PEST Emerald Luc Short life ELuc PEST 3 0 0E 00 5 0E 04
9. RNA Background reduction signal AATAAA 2 SV40 early poly A signal 3 p 7 4 1 pELuc test pELuc PEST test high fidelity PCR KOD Plus Code No KOD 201 KOD Plus Ver 2 Code No KOD 211 PCR
10. D Iuciferin KR 2 BANTHENG HA Emerald Luc Short life Code No ELV 201 C PEST Emerald Luc Short life Code No ELV 201 Emerald Luc Code No ELV 101 ARES ELEH KEN vitro HTS In vitro Emerald Luc in vitro Code No Emerald Luc Luciferase Assay Reagent ni FLATON 96 100
11. 24 0 2 mM D luciferin 2 5Er06 ELuc 2 0E 06 ie unns ce n RA gt 15E 06 l 1 0E 06 fLuc A 5 0E 05 0 0E 00 120 100 80 4 60 OT turaga Tee el 20 0 ELuc fLuc B 0 20 40 60 80 100 120 min Emerald Luc 538nm pH PMT CCD 2 Short life Li ornithine dehydrogenase PEST
12. Emerald Luc ELuc fLuc NIH3T3 0 2 mM D luciferin 5 SV40 Emerald Luc ELuc fLuc CHO ELuc Emerald Luc Luciferase Assay Reagent Code No ELA 101 fLuc E B 10 100 2 0E 04 1 5E 04 1 0E 04 NAM EE HSVtk 5 0E 03 0 0E 00 ELuc fLuc 4 SV40 HSVtk Emerald Luc ELuc fLuc HeLa S3
13. h 7 0E 06 4 r 1 0E 06 8 TEM amp 0E 05 pELuc test pELuc PEST test 5 0E 06 n p E Bmall E wees AL 6 0E 05 E NIH3T3 e 5 100nM El 4 0E 05 Hi 0 2mM D luciferin W 20E 06 3 3 ELuc 2 0E 05 4 ELuc PEST d 0 0E 00 0 0E 00 0 48 72 Time hr 3 Emerald Luc MultiReporter Assay System Tripluc Emerald Luc VEF p 8 SV40 late poly A signal RNA Polymerase Il 200 250 bp
14. 7 2486 2101 1 1 1 3613 1 1 1 1 52 1 1 2 1 1 1 2428 1400 1711 40 2162 89 1850 Bst XI 83 Cfr 91 2101 Cg 16 Eco 521 58 Eco 811 484 285 1719 1719 1857 1611 2365 ND a Nae NNN ee 2 1 1 1 2 1 Bgl M 1 46 2 1 1 2 2 1 co RI 70 PstI 76 1805 10 2 pELuc PEST test Aat 1 Csp Pac Aat Il Dra WI Pvull Afl T Eco 1051 SanD I Apal co4 Sfil Bbr PI Eco T221 Sfl Bfr Mro 1 Bsi WI Msc Il Bss HII Nde Bst EII Nrul Csp45I Nsp V pELuc PEST test 1 81 161 241 321 401 481 561 641 121 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2721 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 ctcgaggtcg gcccaccacc agatgctgta taccaggagt gatctgtgca acgaggacta ctgcctaagg gggctgogag ctgtggacca atctgtgtga gccattcttc ttaaccagga ctgtccaaga ggaggtggcc ccaacatcca gacaggaaca tgtgaacaac aggacgagta gaggagatcc agccggcttc actccaagta ctgagggagg gctgcccatg tgccattctt aaaaaatgct caacaattgc gtggtatgga taatattgaa tgtttttgct tggatctcaa ctatgtggcg ggttgagtac tgagtgataa ggggatcatg gcctgtagca actggatgga ggagcCggtg cacgacgggg aactgtcaga atcctttttg caaaggatct tttgtttgcc cttctagtgt accagtggct ggtcgggctg gagctatgag gcgcacgagg
15. Kpnl ori Poly A 3873 EcoRI 70 pELuc PEST test 4352 bp ELuc Emerald Luc Amp r PEST Poly A 5 Xba 11850 90412486 Not 1 1856 Sspl2162 Sac 2089 BamH 1219 Sac Il 2095 ma 2101 ELuc 6 1 pELuc test 1 pELuc 1 2 Acc 2 7 1110 Eco RV 1 64 Pvul 1 2467 Alw 441 2 2167 3413 Ehel 1 1546 Sac 1 1959 Asel 1 2663 Fsp 1 1 2614 Sac Il 1 1965 Bam HI 1 1977 Hae ll 2 1546 3483 Sall 1 7 Ban Ill 1 16 Hin 11 2 1546 2298 Scal 1 2356 Bell 2 932 1400 Hind I 1 22 Sma 1 1 1971 Bell 1 2715 Kpn 1 1 3743 Spe I 1 52 Bgl M 1 46 Milu 1 28 Sph 1 1 40 Bst XI 2 83 285 Nar 1 1546 Ssp I 1 2032 Cfr 91 1 1971 Neo 1 1 89 Styl 1 89 Cg 1 1 16 Nhe 1 1 34 Xbal 1 1720 Eco 521 2 58 1727 NotI 1 1726 Xhol 1 1 Eco 811 2 484 1611 Po MI 1 1257 Xmn 1 1 2235 Eco RI 1 70 PstI 1 76 2 pELuc test Aat 1 Aat Il Afl II Apal Bbr PI Bfr Bou1102 Bsi WI Bss HII Bst EII Csp45 I Csp 1 Dra II Eco 1051 Ec
16. Ready Blue 100 PIK 251 lt gt E E Zu SLGOR F primer in vitro Emerald Luc 10ml ELA 101 Luciferase Assay Reagent in vitro 201 Emerald Luc Lysis Solution Laden PEN in vivo 20 mg MRL 101 D luciferin 20 mg x5 MRL 101X5 MultiReporter Assay System Tripluc pSLG test 2040 dtd MultiReporter Assay System Tripluc pSLO test 20g MERIVA W MultiReporter Assay System Tripluc 204g MRV 103 pSLR test ultiReporter Assay System Tripluc mm M SV40 pSLG SV40 control 20ug MEE MultiReporter Assay System Tripluc SV40 pSLO SV40 control 20ug Mene MultiReporter Assay System Tripluc SV40 pSLR SV40 control 204g MEUA MultiReporter Assay System Tripluc HSVtk pSLG HSVtk control 20ug MIS Tripluc 037 y EXE 10ml MRA 301 Tripluc Luciferase Assay Reagent 10mlx5 MRA 301X5 14 HPCR REEN
17. signal PCR MultiReporter Assay System Tripluc pSLG pSLO pSLR SLGOR F primer Code No MRV 401 2 Emerald Luc Emerald Luc 2 1 vivo 0 2 1 mM D luciferin Code No MRL 101
18. gatttttgtg acggtatcga atggagagag caacgctctg tcttcgacac gagaacaaca tatcccagac ttttggaggt agcctgcaca ggtagccgoc gactcacaca cacgccttog ggtgttcctg gccctctggt gagatcgcog tactctgcac CCEgCgaggc ccacaggcta tttctacatc tccttcagca gtggtgaagc CCtgaggggc ccctgatgga tcttgtgcoc ctagagcggc ttatttgtga attcatttta gctcccgcgg aaaggaagag cacccagaaa cagcggtaag cggtattatc tcaccagtca Cactgcggoc taactcgcct atggcaacaa ggCggataaa agcgtgggtc agtcaggcaa ccaagtttac ataatctcat tcttgagatc ggatcaagag agccgtagtt gctgccagtg aacggggggt aaagcgccac gagcttccag atgctcgtca taagcttacg agaagaacgt cacaagcact tactgtgaag agagattctt gagctgtgta taaagacaga acttcatgtc atcctgtgct cgcatctgac gcttcagtat aaggccatoc ggacaagtac tgaagaggct aacgagttca cctgggtcca ctaaggaggc gtggaccggt cccaggcatc agcccggcgc ggcgtaaggt gaaggcttct aggagagcgg cgcccagaca aatttgtgat tgtttcaggt CCCgggggat tatgagtatt cgctggtgaa atccttgaga ccgtattgac cagaaaagca aacttacttc tgatcgttgg cgttgcgcaa gttgcaggac tcgcggtatc ctatggatga tcatatatac gaccaaaatc ctttttttct ctaccaactc aggccaccac gcgataagtc tcgtgcacac getteeegaa ggggaaacge ggggggcgga cgtgctagcg ggtgtacggc CCCacatccc ctgggccaga catccccatc aagtgaccgg accaactaca caggtactcc cctccggcac cccagagtgg caacctgggc aggactacga gacctatcca gaacctgcca agtccggctc aaccaggtgg catcgacgac acaaggagct agggacgtgg ccaactcacc tcgtg
19. 10 04 HPCR Emerald Luc Vector Series pELuc test Code No ELV 101 pELuc PEST test Code No ELV 201 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3629K 1 2 3 4 6 7 8 9 3 Emerald Luc 4 1 CN PEE Me E TCR EE CEU eten eene de CEPR ICE EE 4 2 Short life 5 3 oe 5 See 7 4 7 I ra Kanal kanal AP ja Aa BAKA 7 al MM 8 omo 9 13 Tr mar ME 13 14 AE
20. 1121 ccaacatcca tactctgcac aacgagttca agtccggctc cctgggcaag gtgacacctt acatggccgc caagatcatc 1201 gacaggaaca ccggcgaggc cctgggtcca aaccaggtgg gcgagctgtg catctgggga cctatggtaa caaaaggcta 1281 tgtgaacaac ccacaggcta ctaaggaggc Catcgacgac gacggctggc tgcactctgg cgacttcggc tactacgacg 1361 aggacgagta tttctacatc gtggaccggt acaaggagct gatcaaatac aagggctatc aggtcgcccc tgtggagctg 1441 gaggagatcc tccttcagca cccaggcatc agggacgtgg ccgtcgtggg tatccctgac atcgaggccg gcgagctgcc 1521 agccggcttc gtggtgaagc agcccggcgc Ccaactcacc gctaaggagg tgtacgactt cctggcccag agggtgtctc 1601 actccaagta cctgaggggc ggcgtaaggt tcgtggactc tatccccagg aacgtgacag gcaagattag tcgaaaagag 1681 ctgagggagg ccctgatgga gaaggcttct aagctgtaat ctagagcggc Cgcccagaca tgataagata cattgatgag 1761 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgoctt tatttgtaac 9 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2721 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 cattataagc ttttttaaag atgagacaat tattcccttt agttgggtgc tttccaatga tcgccgcata taagagaatt aaggagctaa cataccaaac ttactctagc ccggctggct tggtaagccc agataggtgc catttttaat ccactgagcg aaacaaaaaa ttcagcagag gcctacatac caagacgata acctacaccg tccggtaagc tcgggtttcg aacgcggcct ccataccaca caattgttgt gcattttttt taccacattt ttgttgttgt tttttttcac tgcaataaac caagtaaaac aaccctgata tttgcggcat acgagtgggt tgagcacttt cactattctc atgcagtgct ccgctt
21. c tcgcggtatc ctatggatga tcatatatac gaccaaaatc ctttttttct ctaccaactc aggccaccac gcgataagtc tcgtgcacac gcttcccgaa ggggaaacge ggggggCgg4 gttctttcct tccccctgaa agcaatagca atcttatcat ccctgaacct aatagcatca ttatcatgtc 1 pELuc PEST 1 2 tcagggggag cctcaaatat caacatttcc agtaaaagat gttttcgccc gccgggcaag tcttacggat tgacaacgat gaaccggage actattaact cacttctgcg attgcagcac acgaaataga tttagattga ccttaacgtg gegegtaate tttttccgaa ttcaagaact gtgtcttacc agcccagctt gggagaaagg ctggtatctt gcctatggaa gcggtaccga cctgaaacat tcacaaattt gtctggatca gaaacataaa caaatttcac tg gtgtgggagg gtatccgctc gtgtcgccct gctgaagatc cgaagaacgt agcaactcgg ggcatgacag Cggaggaccg tgaatgaagc ggcgaactac ctcggcoctt tggggccaga cagatcgctg tttaaaactt agttttcgtt tgctgcttgc ggtaactggc ctgtagcacc gggttggact ggagegaacg cggacaggta tatagtcctg aaacgccagc tcataatcag aaaatgaatg cacaaataaa taatcagcca atgaatgcaa aaataaagca Acc 2 7 Alw 441 2297 Asel 2793 Bam HI 2107 Ban Ill 16 Bell 932 Ball 2845 1110 3543 Eco RV Ehel sp Hae ll Hin 1I Hind III KpnI Miul Nar Neo 1 Nhe Not PpuM I 64 1546 2744 1546 1546 22 3873 28 1546 89 34 1856 1257 Pvul Sac I Sac Il Sall Scal Smal Spe I Sph 1 Ssp 1 Styl Xbal Kho Xmn 2597 2089 2095
22. cataaa caaatttcac tg 12 tcataatcag ccataccaca tttgtagagg ttttacttgc aaaatgaatg caattgttgt tgttaacttg tttattgcag cacaaataaa gcattttttt cactgcattc tagttgtggt taatcagcca taccacattt gtagaggttt tacttgcttt atgaatgcaa ttgttgttgt taacttgttt attgcagctt aaataaagca tttttttcac tgcattctag ttgtggtttg 7 1 PCR GC PCR PCR PCR 2 PCR Denature 98 C 10 5 DMSO PCR PCR p 5 PCR 2
23. gactc aagcttagcc gatggaccgt tgataagata gctattgctt tcagggggag cctcaaatat caacatttcc agtaaaagat gttttcgcoc gccgggcaag tcttacggat tgacaacgat g aaccggagc actattaact cacttctgcg attgcagcac acgaaataga tttagattga ccttaacgtg gcgcgtaatc tttttccgaa ttcaagaact gtgtcttacc agcccagctt ggagaaagg ctggtatctt gcctatggaa 11 catgcagatc cccgagccca ccaggccatc gcctccagaa atctccgcct catctccaag taaagaggat gacaacaacc aaccggcctg gtacacaact tatttcatgg ggtgaggagc CCCtggCgga gggatacggt CCtgggcaag gogagctgtg gacggctggc gatcaaatac ccgtcgtggg gCtaaggagg tatccccagg atggcttccc caccctgcag cattgatgag tatttgtaac gtgtgggagg gtatccgctc gtgtcgccct gctgaagatc cgaagaacgt agcaactcgg ggcatgacag cggaggaccg tgaatgaagc ggCgaactac ctcggcoctt tggggocaga cagatcgctg tttaaaactt agttttogtt tgctgcttgc ggtaactggc ctgtagcacc gggttggact ggagegaacg cggacaggta tatagtcctg aaacgccagc tactagtcgg agcaccctct ctggacgtga ctgtggctac ggtacatcgg CCgatcctgg catcatactg tccaaacatt cctaaaggcg CatcccCggc tgggcctgag gtgatcaacg gctgtgctgt gtggctacgg gtgacacctt catctgggga tgcactctgg aagggctatc tatccctgac tgtacgactt aacgtgacag gCCggaggtg cctgtgcttc tttggacaaa cattataagc ttttttaaag atgagacaat tattcccttt agttgggtgc tttccaatga tcgccgcata taagagaatt aaggagctaa cataccaaac ttactctagc ccggctggct tggtaagccc agataggtgc catttttaat ccactgagcg aaacaaaaaa ttcagcagag gcctacatac caagacgata acctacaccg tccggtaagc tcgggtttcg aacgcggcct cc
24. ggatatcg gggcaacttc tgggcaacga aagatgaacg catggtggtg tcttcaccac gactctgaag caagcctctg tgatgcagac gtatccgtgc agtggtgatg ttccctccac ggagccgctc tctaacagag acatggocgc cctatggtaa cgacttcggc aggtcgoccc atcgaggccg cctggcccag gcaagattag gaggagcagg tgctaggatc CCacaactag tgcaataaac caagtaaaac aaccctgata tttgcggcat acgagtgggt tgagcacttt cactattctc atgcagtgct ccgctttttt gacgagogtg ttcccggcaa ggtttattgc tcccgtatcg ctcactgatt ttaaaaggat tcagacccog accaccgcta cgcagatacc ctcgctctgc gttaccggat aactgagata ggcagggtog ccacctctga ttttacggtt aattcctgca accgccggcg gtccoctttcc atgtcgtgtc gcccctgtga taggaagatc agaacctgct cactacgacc ccacaggaac tggcctacct ctccgaaggt aatcctgttc ctctggcgaa tctacctccg caagatcatc caaaaggcta tactacgacg tgtggagctg gegagetgee agggtgtctc tcgaaaagag ctgctggcac aatgtgtaga aatgcagtga aagttaacaa ctctacaaat aatgcttcaa tttgccttcc tacatcgaac taaagttctg agaatgactt gccataacca gcacaacatg acaccacgat caattaatag tgataaatct tagttatcta aagcattggt ctaggtgaag tagaaaagat CCagCggtgg aaatactgtt taatcctgtt aaggcgcagc cctacagcgt gaacaggaga cttgagcgtc cctggccttt 3841 3921 4001 4081 4161 4241 4321 tgctggoctt tttaaaaaac cttataatgg ttgtccaaac aaaaaacctc ataatggtta tccaaactca ttgctcacat gttctttcct ctcccacacc tccccctgaa ttacaaataa agcaatagca tcatcaatgt atcttatcat ccacacctcc ccctgaacct caaataaagc aatagcatca tcaatgtatc ttatcatgtc gcggtaccga cctgaaacat tcacaaattt gtctggatca gaaa
25. o41l Eco T221 Mrol Msc 1 ge 1 Nru 1 Nsp V Pac Pvu II SanD I Sfil Srfl pELuc test 1 ctcgaggtcg acggtatcga taagcttacg cgtgctagcg catgcagatc tactagtcgg ccggatatcg aattcctgca 81 gcccaccacc atggagagag agaagaacgt ggtgtacggc cccgagccca agcaccctct gggcaacttc accgccggcg 161 agatgctgta caacgctctg cacaagcact cccacatccc ccaggccatc ctggacgtga tgggcaacga gtccctttcc 241 taccaggagt tcttcgacac tactgtgaag ctgggccaga gcctccagaa ctgtggctac aagatgaacg atgtcgtgtc 321 gatctgtgca gagaacaaca agagattctt catccccatc atctccgcct ggtacatcgg catggtggtg gcccctgtga 401 acgaggacta tatcccagac gagctgtgta aagtgaccgg catctccaag ccgatcctgg tcttcaccac taggaagatc 481 ctgcctaagg ttttggaggt taaagacaga accaactaca taaagaggat catcatactg gactctgaag agaacctgct 561 gggctgcgag agcctgcaca acttcatgtc caggtactcc gacaacaacc tccaaacatt caagcctctg cactacgacc 641 ctgtggacca ggtagccgcc atcctgtgct cctccggcac aaccggcctg cctaaaggcg tgatgcagac ccacaggaac 721 atctgtgtga gactcacaca cgcatctgac cccagagtgg gtacacaact catccccggc gtatccgtgc tggcctacct 801 gccattcttc cacgccttcg gcttcagtat caacctgggc tatttcatgg tgggcctgag agtggtgatg ctccgaaggt 881 ttaaccagga ggtgttcctg aaggccatcc aggactacga ggtgaggagc gtgatcaacg ttccctccac aatcctgttc 961 ctgtccaaga gccctctggt ggacaagtac gacctatcca ccctggcgga gctgtgctgt ggagccgctc ctctggcgaa 1041 ggaggtggcc gagatcgccg tgaagaggct gaacctgcca gggatacggt gtggctacgg tctaacagag tctacctccg
26. tttt gacgagcgtg ttcccggcaa ggtttattgc tcccgtatcg ctcactgatt ttaaaaggat tcagacccog accaccgcta cgcagatacc ctcgctctgc gttaccggat aactgagata ggCagggtcg ccacctctga ttttacggtt tttgtagagg tgttaacttg cactgcattc gtagaggttt taacttgttt tgcattctag 2 pELuc PEST test aagttaacaa ctctacaaat aatgcttcaa tttgccttcc tacatcgaac taaagttctg agaatgactt gccataacca gcacaacatg acaccacgat caattaatag tgataaatct tagttatcta aagcattggt ctaggtgaag tagaaaagat ccagcggtgg aaatactgtt taatcctgtt aaggcgcagc cctacagcgt gaacaggaga cttgagcgtc cctggccttt ttttacttgc tttattgcag tagttgtggt tacttgcttt attgcagctt ttgtggtttg caacaattgc gtggtatgga taatattgaa tgtttttgct tggatctcaa ctatgtggcg ggttgagtac tgagtgataa ggggatcatg gcctgtagca actggatgga ggagccggtg cacgacgggg aactgtcaga atcctttttg caaaggatct tttgtttgcc cttctagtgt accagtggct ggtcgggctg gagctatgag gegeacgagg gatttttgtg tgctggoctt tttaaaaaac cttataatgg ttgtccaaac aaaaaacctc ataatggtta tccaaactca attcatttta gCtCCCgCgg aaaggaagag cacccagaaa cagcggtaag cggtattatc tcaccagtca CactgcggCC taactcgcct atggcaacaa ggCggataaa agcgtgggtc agtcaggcaa ccaagtttac ataatctcat tcttgagatc ggatcaagag agccgtagtt gctgccagtg aacggggggt aaagcgccac gagcttccag atgctcgtca ttgctcacat ctcccacacc ttacaaataa tcatcaatgt ccacacctcc caaataaagc tcaatgtatc tgtttcaggt cccgggggat tatgagtatt cgctggtgaa atccttgaga ccgtattgac cagaaaagca aacttacttc tgatcgttgg cgttgcgcaa gttgcagga
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