取扱説明書
Contents
1. PCR 42 C 50 C 7 PCR cDNA PCR cDNA ReverTra Ace qPCR RT Master Mix DNA PCR cDNA ReverTra Ace qPCR RT Master Mix with gDNA Remover 200 FSQ 301 PCR 1ml x 1 40 QPS 101T PCR 167ml x 3 THUNDERBIRD Probe qPCR Mix PS 101 1 200 1ml x 12. Nuclease free Water Nuclease free DEPC 3 37 C 98 C 65 C Nuclease free Water 200 RNA DEPC Nuclease free Water DEPC DEPC DEPC
3. PCR Nuclease free Water Total RNA Total RNA Total RNA mRNA 1 2 Total RNA RNA AGPC Acid Guanidium Phenol Chloroform Total RNA DNA DNA DNase DNA p 5 DNA ReverTr
4. 0 5pmolul 10ul 5pmol 3 37 C 15 98 C 5 4 C 20 C PCR RNA PCR 20 PCR
5. PCR PCR 42 50 C 5 1 Total RNA DNase AGPC Total RNA DNA DNA p 3 DNA DNA ReverTra Ace qPCR RT Master Mix with gDNA Remover Code No FSQ 301 1 Total RNA lt 10hg X HI 10x DNase Buffer 1 HI 10mM Tris Cl pH7 6 2 5mM MgCb 0 5mM CaCb RNase free DNase 10U ul 0 5 Hl Nuclease free Water to 10 Hl 10 30 2 100ul Nuclease free Water 100ul
6. cDNA PCR PCR PCR THUNDERBIRD qPCR Mix 7 1 ReverTra Ace qPCR RT Kit PCR cDNA 2 ReverTra Ace qPCR RT Kit 15 PCR RNA RNase H 3 PCR ReverTra Ace qP
7. RNase RNA RNA RNA 0 5pg 1ug RNA RNA RNA RNA RNA PCR 20 PCR
8. SYBRY Green PCR 40 QPS 201T THUNDERBIRD SYBR qPCR Mix LO a 200 THUNDERBIRD2 qPCR Mix 50X ROX reference dye 50ul 1000 QPS 101X5 QPS 201X5 QPS 101 QPS 201 5 RNA 100 NPK 201F Total RNA MaqgExtractor RNA Maqg cg Trapper 1 MGS 101 http www toyobo co jp bio HPCR 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail or
9. CR RT Kit PCR 20 PCR Realtime PCR Master Mix mRNA 2 10ul 200 20 C Reacton Bufe MgCl dNTPs 400HI Rr tre Acss RNase inhibitor 20C 100ul Randem Primer Ogo dT pmmey 20 C 1 Nuclease free Water 20 C 1000ul x2 5x RT Buffer MgCl dNTPs 5x Enzyme Mix ReverTra Ace RNase inhibitor Primer Mix Random Primer Oligo dT Primer
10. TE 5 12 000rpm 5 100ul 12 000rpm 5 100ul 5M 200ul 20 C 30 5 70 12 5 a Nuclease free Water 5 6 1 PCR RNA RNA RNA RNA RNase RNA RNA RNA
11. a Ace qPCR RT Master Mix with gDNA Remover Code No FSQ 301 poly Aj RNA mRNA poly A RNA oligo dT poly A mRNA mRNA mRNA Total RNA RNase ribosomal RNA 4 1 RNA RNA 65 C 5 RNA 5x RT Buffer 2 Nuclease free Water to 10 Hl 5x RT Buffer 2 HI RT Enzyme Mix 0 5 Hl Primer Mix 0 5 Hl RNA 0 5p9 1hg Total volume 10 Hl Primer Mix Gene Specific Primer
12. der_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
13. ldeas amp Chemistry ReverTra Ace qPCR RT Kit Code No FSQ 101 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3736K 1 2 3 4 5 6 7 1 ReverTra Ace qPCR RT Kit PCR cDNA ReverTra Ace PCR cDNA PCR cDNA
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