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Probe qPCR Mix

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2. PCR 95 C 30 3 Smart Cycler System http www takara bio co jp 15 RR391A PCR gt em O 95 30 DNA OFF 95C 1 2 2 AR 1B 7 307 45 PCR 300 bp 95 3 5
3. Probe qPCR Mix 2Xconc 20 TaqMan http www takara bio co jp 5 RR391A VI Thermal Cycler Dice Real Time System III Zite X Thermal Cycler Dice Real Time System 1 PCR 1 gt Probe qPCR Mix 2X PCR Forward Primer 10 u M PCR Reverse Primer 10 u M Taq
4. 20 ul DNA template 100 ng RT PCR cDNA RT template PCR 10 5 http www takara bio co jp 8 RR391A 2 PCR PCR PCR 16 PCR 7300 Real Time PCR System gt PCR Stage 1 Reps 1 95 C 30 Stage 2 PCR Reps 40 95 5 60 31 StepOnePlus Real Time PCR System gt Fast Holding Stage Reps 1 95 20 Cycling Stage Number of Cycles 40 95 1 60 20 DNA
5. LightCycler 480 System http www takara bio co jp 13 RR391A CFX96 Real Time PCR Detection System CFX96 Real Time PCR Detection System 1 PCR 1 gt Probe qPCR Mix 2X 10 ul 1x PCR Forward Primer 10 u M 04 ul 02 u M PCR Reverse Primer 10 u M 04 ul 0 2 uM TagMan 0 8 ul 2 template 2 ul 3 dH20 64 ul Total 20 ul 1 0 2 uM 0 1 1 0 uM x 2 PCR 3 template
6. http www takara bio co jp 11 RR391A LightCycler 96 System X LightCycler 96 System 1 PCR 1 gt Probe qPCR Mix 2X 10 ul 1x PCR Forward Primer 10 u M 04 ul 02 u M PCR Reverse Primer 10 u M 04 ul 0 2 uM TagMan 0 8 ul 2 template 2 ul 3 dH20 64 ul Total 20 ul 1 02 uM 0 1 1 0 uM x 2 PCR 3 template DNA template 100 ng RT PCR cDNA RT template
7. PCR 16 TPCR 7500 Real Time PCR System gt PCR Stage 1 Reps 1 95 C 30 Stage 2 PCR Reps 40 95 5 60 34 7500 Fast Real Time PCR System gt Fast Holding Stage Reps 1 95 20 Cycling Stage Number of Cycles 40 95 3 60 30 DNA 7gg PCR PCR PCR 95 C 5 15 PCR 95 C 30 3
8. http www takara bio co jp 2 RR391A 2 TagMan 5 FAM T 3 TAMRA TaqMan DNA DNA 5 3 exonuclease TaqMan 1 2 e j Tuy v 4 3 e es 7 E gt aby Ar ER http www takara bio co jp 3 RR391A I 200 Probe qPCR Mix 2x conc 1 1mlx5 ROX Reference Dye 50x conc 200 ul ROX Refe
9. 56 20 30 64 31 34 5 Life Technologies 30 7300 Real Time PCR System 31 7500 Real Time PCR System 34 Vil 1 2005 Y 3 4 PCR p120 126 2 2008 PCR 3 1 RT PCR p39 43 Vil PrimeScript RT reagent Kit Perfect Real Time RRO37A B PrimeScript RT reagent Kit with gDNA Eraser Perfect Real Time RRO47A B PrimeScript RT Master Mix Perfect Real Time RR036A B One Step PrimeScript RT PCR Kit Perfect Real Time RR064A B SYBR Premix Ex Taq 11 Tli RNaseH Plus RR820S A B SYBR Premix Ex Tag Tli RNase
10. 20 30 30 16 PCR 2 Step PCR Fattern Segment PCR Hold Cycle 1 95 30 2 Step PCR Cycle 40 95 5 60 30 Temperature deg Hold Time mm ss Data Collection DNA 7gg PCR PCR PCR 95 5 15 PCR 95 C 30 3 Thermal Cycler Dice Real Time System Ill 43 amp U Li
11. 3 CFX96 Real Time PCR Detection System http www takara bio co jp 14 RR391A Smart Cycler Il System X Smart Cycler System 1 PCR 1 gt Probe qPCR Mix 2X 12 5 ul 1x PCR Forward Primer 10 u M 0 5 ul 0 2 uM 1 PCR Reverse Primer 10 u M 0 5 ul 0 2 uM TaqMan 1 ul 2 template 2 ul 3 dH20 85 ul Total 25 ul 1 02 uM 0 1 1 0 uM 2 PCR Smart Cycler System Smart Cycler Il System
12. PCR 10 2 PCR PCR PCR 16 PCR PCR Hold Cycle 1 95 30 2 Step PCR Cycle 40 95 5 60 30 DNA 7gg PCR PCR PCR 95 C 5 15 PCR 95 C 30 3
13. LightCycler 96 System http www takara bio co jp 12 RR391A LightCycler 480 System LightCycler 480 System 1 PCR 1 gt Probe qPCR Mix 2X 10 ul 1x PCR Forward Primer 10 u M 04 ul 02 u M PCR Reverse Primer 10 u M 04 ul 0 2 uM TagMan 0 8 ul 2 template 2 ul 3 dH20 64 ul Total 20 ul 1 02 uM 0 1 1 0 uM 2 PCR 3 template DNA template 100 ng
14. 7gg PCR PCR PCR 95 C 5 15 PCR 95 C 30 3 http www takara bio co jp 9 RR391A Applied Biosystems 7500 7500 Fast Real Time PCR System Life Technologies 1 PCR lt 1 gt Probe qPCR Mix 2X PCR Forward Primer 10 u M PCR Reverse Primer 10 u M TagMan ROX Reference Dye Il 50x template dH20 Total 10 ul 04 ul 04 ul 0 8 ul 02 ul 2 ul 62 ul 20 ul 5 25 ul 1x 1
15. DNA template 100 ng RT PCR cDNA RT template PCR 1096 2 PCR PCR PCR 16 PCR PCR Hold Cycle 1 95 30 2 Step PCR Cycle 40 95 5 60 30 DNA 7gg PCR PCR PCR 95 5 15 PCR 95 C 30
16. 0 1 0 5 uM 3 template DNA template 100 ng RT PCR cDNA RT template PCR 10 2 Smart Cycler PERTHE CED Smart Cycler PCR PCR PCR 16 PCR PCR Stage 1 Hold 95 30 Stage 2 PCR Repeat 40 times 95 5 60 20 DNA 7gg PCR PCR PCR 95 C 5 15
17. 6 20 20 4 6 http www takara bio co jp 4 RR391A IV PCR 2 PCR 2X RNase H Tli RNaseH cDNA mRNA PCR GEJ V dh
18. RT PCR cDNA RT template PCR 1096 2 PCR PCR PCR 16 PCR PCR Hold Cycle 1 95 30 2 Step PCR Cycle 40 95 5 60 30 DNA 7gg PCR PCR PCR 95 5 15 PCR 95 C 30 3
19. ul 0 2 uM 1 1ul 0 2 uM 1 2 ul x2 0 5 ul Q 5x 3 4 ul 4 16 5 ul 50 pul 5 1 0 2 uM 0 1 1 0 uM x 2 PCR 3 ROX Reference Dye Il 50X 0 5x 4 template 20 pl DNA template 100 ng RT PCR cDNA RT template PCR 10 5 http www takara bio co jp 10 RR391A 2 PCR PCR
20. H Plus RR4205 A B SYBR Fast qPCR Mix RR430A One Step SYBR PrimeScript PLUS RT PCR Kit Perfect Real Time RR096A B Thermal Cycler Dice Real Time System II TP950 TP970 TP980 TP990 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Smart Cycler Il System SC200N SC210N http www takara bio co jp 16 RR391A IX xr Thermal Cycler Dice TaqMan Roche Diagnostics GmbH SYBR Molecular Probes Inc Premix Ex Taq PrimeScript
21. Man template dH20 Total 1x 02 uM 02 uM 2 3 1 0 2 uM 0 1 1 0 uM x2 PCR Thermal Cycler Dice Real Time System Ill U Lite 0 1 0 5 uM 3 template DNA template 100 ng RT PCR cDNA RT template PCR 1096 http www takara bio co jp 6 RR391A 2 PCR PCR
22. ma RR391A Taltana Probe qPCR Mix v201509Da Probe qPCR Mix h TagMan PCR qPCR lt PCR PCR PCR 2 RNase H Tli RNaseH 2 X cDNA mRNA PCR PCR PCR Thermal Cycler Dice Real Time System III TP950 TP970 TP980 TP990 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Applied Bi
23. osystems 7300 7500 7500 Fast Real Time PCR System StepOnePlus Real Time PCR System Life Technologies LightCycler 96 480 System Roche Diagnostics CFX96 Real Time PCR Detection System Bio Rad Smart Cycler System Smart Cycler Il System Cepheid 1 The 5 nuclease process is covered by patents owned by Roche Molecular Systems Inc and F Hoffmann La Roche Ltd Purchase of the product does not provide a license to use this patented technology 2 SYBR Green SYBR Greenl SYBR Premix Ex Taq II Tli RNaseH Plus RR820A SYBR Premix Ex Tag Tli RNaseH Plus RR420A SYBR Fast qPCR Mix RR430A DNA PCR PCR TaqMan 1 PCR PCR DNA DNA DNA 3 1 100
24. rence Dye II 50X conc 200 ul 1 PCR dNTP Mixture Mg2 Tli RNase H 2 Life Technologies PCR ROX Reference Dye 50X 1X 7300 Real Time PCR System StepOnePlus Real Time PCR System Life Technologies ROX Reference Dye II 50X 0 5X 7500 Real Time PCR System 7500 Fast Real Time PCR System Life Technologies Thermal Cycler Dice Real Time System TP950 TP970 TP980 TP990 TP900 TP960 TP700 TP760 LightCycler 96 480 System Roche Diagnostics 4t CFX96 Real Time PCR Detection System Bio Rad Smart Cycler System Smart Cycler Il System Cepheid PCR authorized instruments PCR TaqMan licensed probe QUU ds Dh I 4
25. te ER http www takara bio co jp 7 RR391A Applied Biosystems 7300 Real Time PCR System StepOnePlus Real Time PCR System Life Technologies 1 PCR lt 1 gt Probe qPCR Mix 2X PCR Forward Primer 10 u M PCR Reverse Primer 10 u M TagMan ROX Reference Dye 50x 3 template dH20 Total o 10 ul 04 ul 04 ul 0 8 ul 04 ul 2 ul 6 ul 20 ul 5 25 ul 1x 1ul 0 2 uM 1 1 ul 0 2 u M 2ul 2 1ul 1x 4 ul 4 16 ul 50 pul 5 1 02 uM 0 1 1 0 uM 2 PCR 3 ROX Reference Dye 50X 1X 4 template

Probe qPCR Mix

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