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CD8-FITC/CD4-RD1/CD3-PC5

1. Fluorescein isothyocyanate FITC Phycoerythrin PE Phycoerythrin Cy5 PC5 2 CYTO COMP CYTO COMP Cell 4 Quadrant2 triCHROME CD8 FITC CD4 RD1 CD3 PC5 COULTER CYTO TROL COULTER CYTO TROL
2. 1 SS 90 LS FS 2 3 FS SS 1 Cyto Stat COULTER CLONE Mo2 RD1 KC56 FITC 1 SS FS 2 Gate A MHULE BLOOD FS 2H 400 3 68080 88801888 8 208 4808 688 SS 888 1888 2 MslgG1 PC5 FL3 Log FL4 Log MslgG1 RD1 FL2 Log 2 2 Quadlant 3 98 4
3. triCHROME CD8 FITC CD4 RD1 CD3 PC5 3 Cyto Stat COULTER CLONE Mo2 RD1 KC56 FITC triCHROME SIgG1 FITC MSIgG1 RD1 MslgG1 PC5 COULTER IMMUNOPREPTV Reagent System Q Prep Multi Q PrepTWM TQ Prep IM FS SS 4
4. triCHROME CD8 FITC CD4 RD1 CD3 PC5 6607053 50 0 5mL RD1 4 520 110 PC5 4 520 110 4 542 104 Cy5 4 981 977 5 268 486 Pe Schlossman SF Boumsell L Gilks W Harlan JM Kishimoto T Morimoto C Ritz J Shaw S Silverstein R Springer R Tedder TF and Todd RF eds 1995 Leukocyte Typing V Oxford UK Oxford University Press pp 262 263 268 2 Bernard A Boumsell L Dausset J Milstein C and Schlossman SF eds 1984 Leukocyte Typing New York NY Springer Verlag pp 28 41 42 44 196 3 McMichael AJ ed 1987 Leukocyte Typing lll Oxford UK Oxford University Press pp 38 40 42 43 116 167 170 172 176 199 206 302 308 315 475 4 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T lymphocytes Cell 19 821 827 5 Aiuta F Cerottini J C Coombs RRA Cooper M Dickler HB Froland Fudenberg HH Greaves MF Grey HM Kunkel HG Natvig J Preud homme J L Rabellino E Ritts RE Rowe DS Seligmann M Siegal FP Stjernsward J Terry WD and Wybran J 1975 Identification enumeration and isolation of B and T lymphocytes from human peripheral blood International Union of Immunological Societies IUIS Report July 1974 Clin Immunol
5. 4 10 12 5 TQ Prep Multi Q Prep Q Prep 6 a b FS SS
6. Fc CD45 CD14 8X3 Cyto Stat COULTER CLONE Mo2 RD1 KC56 FITC triCHROME MSlgG1 FITC MSlgG1 RD1 MSlgG1 PC5 Fc Quadrant 3 989 Quadrant 1 2 4 2 2
7. 5 6 lt 7 GLP Good Laboratory Practices CV 5 6 8 9 DNE MI WES Ro ANO EO Ni 10 e triCHROME CD8 FITC CD4 RD1 CD3 PC5 2 lt 8 12 2 8C 2 8C 90 2 8 C
8. AR CD3 lgG1 FITC T4 RD1 CD3 CD3 CD4 CD3 lgG1 FITC T8 RD1 CD3 CD3 CD8 CD3 y 1 01x 0 68 r 0 992 CD3 CD4 y 0 98x 3 06 r 0 962 CD3 CD8 y 0 92x 1 95 r 0 970 1 0 19 6 2 3 Etz RELH 4
9. EDTA 1 100uL 12mL 909 6 triCHROME triCHROME CD8 FITC CD4 RD1 CD3 PC5 6607053 50 0 5mL COULTER Q PREP COULTER IMMUNOPREP 7546946 COULTER MULTIQ PREP TQ PREP COULTER IMMUNOPREP 7546999 300
10. 11 OKT3 triCHROME CD8 FITC CD4 RD1 CD3 PC5 12 13 triCHROME 10 ux L 20 25 6 20 25 C
11. triCHROME CD8 FITC CD4 RD1 CD3 PC5 CD3 CD4 CD8 CD4 CD3 CD4 CD8 EPICS XL MCL 10 CV CD4 CD8 triCHROME CD8 FITC CD4 RD1 CD3 PC5 CD3 9 CD4
12. 5 6 Oyto Trol 7 8 9 10
13. MslgG1 PC5 FL3 Log FL4 Log MslgG1 FITC FL1 Log 0 2 3 Quadlant 3 989 4 2 MslgG1 PC5 MslgG1 RD1 2 4 LYMPHOCYTE 1808 18088 T A 1 C 18 1808 18088 mIgG1 PC5 3 6 3 MslgG1 PC5 MslgG1 FITC 2 4 LYMPHOCYTE 1808 180880 mIgG1 FITC 1 18 188 180808 mlgG1 PC5 3 4 CD3 PC5 FL4 Log FL3 Log CD4 RD1 FL2 Log RU CD3 PC5 FL4 Log FL3 Log amp CD8 FITC FL1 Log 2 4 5 4
14. triCHROME MSlgG1 FITC MSlgG1 RD1 MSlgG1 PC5 6607054 Cyto Stat COULTER CLONE Mo2 RD1 KC56 FITC 6603909 50 FLOW COUNT CYTO COMP Reagent Kit CYTO COMP Cell Kit 12x 75 mm EDTA 100 50 7547053 20 mL 6607021 4 x 0 5 mL 6607023 5x1 mL 2 6 CYTOMICS FC500 EPICS XL XL MCL LH700 1 3 10 x 10 L 10 x 10 f uL 3 x 10 L Q PREP IMMUNOPREP
15. lg Fc Ig Smlg T B NK TdT HLA DR Smlg T B T B 1 T Pan T CD7 CD2 CD5 CD3
16. Pan T T fW T CD3 CD4 CD8 T T CD3 20kD CD3 T CD3 Pan T T CD4 62kD CD4 T CD4 CD4 T
17. 4 CD3 PC5 CD4 RD1 2 LYMPHOCYTE 1808 18808 CD4 RD1 18 ee n IS o Bc wen 1 1 18 188 1888 CD3 PC5 5 4 CD3 PC5 CD8 FITC 2 LYMPHOCYTE 1808 18808 CD8 FITC 18 1 1 1 18 188 1888 CD PC5 2 1 L L x x 96 10 2 FLOW COUNT FLOW COUNT L FLOW COUNT x FLOW COUNT 18 85 n 56
18. CD3 CD4 CD8 T B Pan B CD19 B pre pre B CD20 pre B CD19 CD20 B B B B 4 6 Pan T Pan B T B
19. AIDS CD4 T4 T8 0 CD8 T4 T8 CD4 CD8 T4 T8 CD4 CD3 zh T CD4 T CD8 80 TT 30 35 NK
20. a gt 10 x 10 L 10 20 x10 1 2 20 30 x 10 1 3 30 40 x10 1 5 40 60 x10 1 6 60 100 x10 1 10 100 200 x10 1 20 b 3 x 10 L 1 25 C 500 xg 2 3 4 LH700 4 10x 10 L 2 12mm x 75mm 3 triCHROME CD8 FITC CD4 RD1 CD3 PC5 triCHROME MSIgG1 FITC MSIgG1 RD1 MSIgG1 PC5 OCyto Stai COULTER CLONE Mo2 RD1 KC56 FITC 10L 3 100 L
21. T B NK null 3 T B T T BB lg T BB E Smlg E T T Smlg B
22. triCHROME CD8 FITC CD4 RD1 CD3 PC5 EPICS XL MCL CD3 CD4 CD8 CD3 CD4 CD3 CD8 909 85 L n Min Max Mean 1SD 96 CD3 56 66 93 79 7 6 1 CD4 56 39 76 54 22 7 7 CD8 56 16 43 28 0 6 1 CD3 CD4 56 38 75 53 5 7 7 CD3 CD8 56 10 40 24 2 6 0 n Min Max Mean 1SD L CD3 56 769 2 045 1 431332 CD4 56 437 1 627 969 2 239 CD8 56 236 895 5022 159 CD3 CD4 56 431 1 618 957 239 CD3 CD8 56 163 789 437 3 150
23. CD4 CD8 CD4 AIDS RATHA HIV HIV CD4 CD4 CD4 T4 T8 CD4 CD8 CD4 CD8 T4 T8 T T T4 T8 T4 T8 CD4 ARC AIDS related complex AIDS
24. T T B T CD4 class I MHC CD8 68kD 32 34kD CD8 809 T 30 3596 NK CD8 CD8 class MHC CD3 CD4 CD8 CD3 CD4 CD8 CD3
25. 9 CD8 9 n Mean 1SD 9 CV Mean 1SD CV Mean 1SD 10 45 5 0 66 1 46 10 90 53 4 84 11 0 0 35 3 16 10 77 91 49 1 91 57 7 1 14 1 97 27 40 81 2 94 10 86 00 65 0 75 74 7 0 52 0 70 64 2 0 34 0 52 EPICS XL XL MCL 3 10 1 3 triCHROME CD8 FITC CD4 RD1 CD3 PC5 CD3 9 CD4 9 CD8 96 n Mean 1SD CV Mean c1SD CV Mean 1SD CV 1 10 72 6 0 56 0 77 52 30 50 0 97 20 9 0 61 2 90 2 10 73 30 71 0 97 53 10 56 1 06 20 8 0 46 2 22 3 10 73 4x0 56 0 76 53 1 0 66 1 24 21 1 0 62 2 94 triCHROME CD8 FITC CD4 RD1 CD3 PC5 nt 50 EPICS XL MCL CD3 CD4 CD8
26. CD3 CD4 RD1 CD3 CD4 PC5 RD1 CD3 CD4 PC5 CD3 CD4 PC5 RD1 3 4 CD3 CD8 FITC CD3 CD8 PC5 FITC CD3 CD8 PC5 CD3 CD8 PC5 FITC 1 6 2 3 909 4
27. 22 23 24 25 2f 28 34 35 Morimoto C Letvin NL Distaso JA Aldrich WR and Schlossman SF 1985 The isolation and characterization of the human suppressor inducer T cell subset J Immunol 134 1508 1515 Morimoto C Letvin NL Distaso JA Brown HM and Schlossman SF 1986 The cellular basis for the induction of antigen specific T8 suppressor cells Eur J Immunol 16 198 204 Meuer SC Schlossman SF and Reinherz EL 1982 Clonal analysis of human cytotoxic T lymphocytes 14 and T8 effector T cells recognize products of different major histocompatibility regions Proc Natl Acad Sci USA 79 4395 4399 Shaw S 1994 Leukocyte Differentiation Antigen Database database Version 1 11 5th International Workshop on Human Leukocyte Differentitation Antigens Available from Stephen Shaw National Institutes of Health on disk NIH ftp site balrog nci nih gov Bethesda MD Reinherz EL Hussey RE Fitzgerald K Snow P Terhorst C and Schlossman SF 1981 Antibody directed at a surface structure inhibits cytolytic but not suppressor function of human T lymphocytes Nature 294 168 170 Caligiuri M Murray C Buchwald D Levine H Cheney P Peterson D Komaroff AL and Ritz J 1987 Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome J Immunol 139 3306 3313 Benjamini E and Leskowitz S Immunology A Short Course Second Edition 1991 New York
28. Wiley Liss pp 211 244 Reinherz EL O Brien C Rosenthal P and Schlossman SF 1980 The cellular basis for viral induced immunodeficiency Analysis by monoclonal antibodies J Immunol 125 1269 1274 Felsenstein D Carney WP lacoviello VR and Hirsch MS 1985 Phenotypic properties of atypical cytomegalovirus induced mononucleosis J 152 198 203 Rinaldo CR Ho M Hamoudi WH Gui X and DeBiasio RL 1983 Lymphocyte subsets and natural killer cell responses during cytomegalovirus mononucleosis Infect Immun 40 472 477 Goldstein G Lifter J and Mittler R Immunoregulatory changes in human disease detected by monoclonal antibodies to T lymphocytes In Monoclonal Antibodies in Clinical Medicine McMichael AJ and Fabre JW eds 1982 New York NY Academic Press pp 39 70 26 Pedrazzini A Freedman AS Andersen J Heflin L Anderson K Takvorian T Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantation for B cell on Hodgkin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 2211 Preijers FWMB DeWitte T Wessels JMC DeGast GC VanLeeuwen E Capel PJA and Haanen C 1989 Autologous transplantation of bone marrow purged in vitro with anti CD7 WTI Ricin A Immunotoxin in Tcell lymphoblastic leukemia and lymphoma Blood 74 1152 1158 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotyp
29. 2009 11 2007 2 21600AMY00089000 6607053 877449 T triCHROME CD8 FITC CD4 RD1 CD3 PC5 1 2 3 4 1 CD8 0 18 g CD4 0 18 g CD3 0 3
30. 1g FITC RD1 PC5 CD3 UCHT1 BALB c Y 2A 028 amp scm NS 1 v 2 A Er B8 CD4 SCFI12T4D11 T4 BALB cJ NS 1 CD8 SCFI21ThyD3 T8 BALB cJ NS 1 lg lgG1 H RU x L CD3 CD4 CD8 CD3 CD4 CD8 CD3 CD4 CD8 R R CD8 FITC Fluorescein isothyocyanate FITC CD4 RD1 Phycoerythrin PE CD3 PC5 Phycoerythrin Cy5 PC5 1 6 FITC 3 10 RD1 0 5 1 5 PC5 05 1 5 FITC 468 509nm 504 541nm RD1 486
31. 580nm 568 590nm PC5 486 580nm 660 680nm BSA 0 296 0 01M 0 15M 0 19 T T T triCHROME CD8 FITC CD4 RD1 CD3 PC5 3 3 CDS CD4 CD8 CD3 CD4 CD3 CD8
32. and Immunopathol 3 584 597 6 Foon KA and Todd RF 1986 Immunologic classification of leukemia and lymphoma Blood 68 1 31 7 Drexler HG Gignac SM and Minowada J 1988 Routine immunophenotyping of acute leukaemias Blut 57 327 339 8 Heinherz EL Haynes BF Nadler LM and Bernstein ID 1986 Leukocyte Typing Il New York NY Springer Verlag Vol 2 pp 8 15 25 9 Barclay AN Birkeland ML Brown MH Beyers AD Davis SJ Somoza Williams AF eds 1993 The Leukocyte Antigen Facts Book London UK Academic Press pp 106 109 10 Reinherz EL Meuer S Fitzgerald KA Hussey HE Levine H and Schlossman SF 1982 Antigen recognition by human T lymphocytes is linked to surface expression of the T3 molecular complex Cell 30 735 743 11 Meuer SC Acuto O Hussey HE Hodgdon JC Fitzgerald KA Schlossman SF and Reinherz EL 1983 Evidence for the T3 associated 90K heterodimer as the T cell antigen receptor Nature 303 808 810 12 Reinherz EL Meuer SC and Schlossman SF 1983 The delineation of antigen receptors on human T lymphocytes Immunol Today 4 5 8 13 Reinherz EL Morimoto C Fitzgerald KA Hussey RE Daley JF and Schlossman SF 1982 Heterogeneity of human T4 inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations J Immunol 128 463 468 14 de Martini RM and Parker JW 1989 Immunologic alterations in human immunodeficiency virus infection A review J Clin Lab Anal 3 56 70 20 21
33. ically defined T helper inducer cells T4 4B4 and increase in T suppressor effector cells T8 2H4 in stable renal allograft recipients Transplantation 47 465 471 lymphocytes in Infect Dis 29 Fauci AS 1988 The human deficiency virus Infectivity and mechanism of pathogenesis Science 239 617 622 30 Taylor MGJ Fahey JL Detels R and Giorgi JV 1989 CD4 percentage CD4 number and CD4 CD8 ratio in HIV infection How to choose and how to use J AIDS 2 114 124 31 Beverley PCL and Callard RE 1981 Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti T cells antibody Eur J Immunol 1 329 334 32 Reinherz EL Kung PC Goldstein G and Schlossman SF 1979 A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells J Immunol 123 1312 1317 33 National Committee for Clinical Laboratory Standards 1991 Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture 3rd Edition H3 A3 National Committee for Clinical Laboratory Standards Villanova PA Loken MR Brosnan JM Bach BA and Ault KA 1990 Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry Cytometry 11 453 459 Gebel HM Lebeck LL Jensik SC Landay AL and Bray RA 6 6 36 37 38 1989 Discordant expression of CD3 and T cell receptor antigens on lymphocytes from patients treated with OKT3 Trans
34. plantation Proceedings 1 1745 1746 Tunnacliffe A Olsson C Traunecker A Krissensen GW Karjalainen K and De Le Hera A The majority of CD3 epitopes are conferred by the epsilon chain In Leukocyte Typing IV White Cell Differentiation Antigens Knapp W Dorken B Gilks WR Rieber EP Schmidt RE Stein H and Kr von dem Borne AEG eds 1989 Oxford Oxford University Press pp 295 296 Schroeder TI and Chatenoud L Immunological monitoring during treatment with OKT3 Presented under the auspices of the American Society of Transplant Surgeons Koepke JA and Landay AL 1989 Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 52 19 27 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 ERE RC JU 135 0063 5 7 TOC oe COULTER 6607053 0911

CD8-FITC/CD4-RD1/CD3-PC5

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