取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. DNA PCR PCR PCR DNA Taq DNApolymerase 2 Pfu DNA polymerase 6 PCR Barnes PCR 1 KOD Dash 3 LDP 101 LDP 101X5 LDP 101X10 250U x 1 KOD Dash 2 5U gl 100lx 1 10x Buffer 1200lx1 LDP 101 x5 LDP 101 x 10 2mM dgNTPs 1000lx1 PCR Mg 1 2mM 2 gt2. PCR gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http Wwww toyobo co jp bio http www toyobo co jp bio Human cDNA PCR ADNA PCR Nested PCR HCV PCR PCR Master Mix Colony DirectLPCR PCR TA GC RT PCR GC PCR RT PCR Yeast Cell RNA RT PCR 4 PCR Endo Arabinase gene Pseudomonas Sp DNA 16kb DNA
3. 3 KOD Dash human genome globin 8 5kb 4DNA 12kb gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobo jp A3683K URL http Wwww toyobo co jp bio 4 PCR 1 PCR Volume Final Concentration 10x PCR Buffer 5 ul 1x KOD Dash 2 5U gl 0 5 1 25 U reaction 1 50 ng Plasmid 1 50 ng cDNA 10 1000 ng Genomic DNA 10uM 1 02uMeach 2mM dNTPs 5 ul 0 2mM Autoclaved distilled water to 50 gl 2 PCR
4. iat Target Number of 2kb 2kb 4kb 4kb Cycles 1 Denaturation HC HC AC 30sec 30sec 30sec 2 Annealing MY J 25 30 2sec 2sec TEN 74 C 74 C 68 70 C 30sec 1min 2kb PCR target 1min 2kb PCR target 5 1 50 ul 1 25U 8kb 2 5U 2 4kb Tm 72 C 3 8kb dgNTPs 0 35mM 4 5 6 PCR
5. PCR 7 2 5ng 25cycle human genome DNA 100ng 30cycle 8 PCR A 1 T vector TA gt TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http Wwww toyobo co jp bio 6 KOD Dash PCR Overnight Pick up PCR PCR 1 1 5hr Pick up Mini Prep
6. DNA PCR PCR UPLOAD Vol 82 Vol 1 PCR gt TOYOBO a 0 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http Www toyobo co jp bio 7 Code No KOD Dash PCR Buffer 1ml LDP 1B dNTPs Mixture 2mM 1 ml NTP 201 lt TA gt TAK 101 TArget Clone lt ReverTra Ace cDNA gt 100 FSK 101 ReverTra Ace lt PCR gt 200 NPK 601 MagExtractor PCR amp Gel Clean up i gt PCR gt InsertCheck Ready 8 1 Imanaka T et al App7 Environ Microbiol 60 4559 4566 1994 2 Imanaka T et al Appl Environ Microbiol 63 4504 4510 1997 3 Imanaka T et al J Biotechnology 88 141 149 2001 4 Barnes W
7. 1 5hr RNase 0 5 1 5hr 1hr 1hr TA TArget Clone pTA2 Vector 4 5kb PCR KOD Dash PCR PCR Primer Vector Primer M 1 2 3 4 1 23 ul 10x Buffer 3 gl 2mM dNTPs 3 gl 4 5kb Forward Primer 10 M 0 35 gl Reverse Primer 10M 0 35 gl KOD Dash 0 3 gl Total30 PCR M 1kb Ladder Marker 1 4 94 C 4 min 1 1 Agarose Gel 94 C 30 sec Tm Tm 5 C 5 sec 30 72 C 1kb 30 sec PCR 1 3 4
8. M Proc Mag7 Acad Sci US4 91 2216 2220 1994 5 Kanzaki H oe and Mineral Res 17 210 220 2002 6 Nakayashiki H et al Genetics 153 693 703 1999 7 Kikuchi A et al Gene 236 293 301 1999 gt FAX 06 6348 3833 TOYOBO i 5 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http Www toyobo co jp bio 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order lifescience toyobo jp gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobo jp URL http Www toyobo co jp bio
9. 14 04 Code No LDP 101 Code No LDP 101 5 Code No LDP 101X10 2 0 C PCR KOD Dash proofFreading 5 Taq DNA polymerase pol I proof readind w Long PCR Bames proof reading KOD DNA polymerase KOD DNA polymerase PCR PCR KOD DNA polymerase PCR PCR DNA polymerase Taq DNA polymerase DNA polymerase 2 KOD Dash KOD DNA polymerase
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