ハイスピードフローサイトメーター CyAnTM ADPを用いた酵母生存率の測定
Contents
1. 1 Smart K Chambers KM Lambert I Jenkins C 1999 Use of Methylene Violet Staining Procedures to Determine Yeast Viability and Vitality Am Soc Brew Chem 57 1 18 23 Zhang T amp Fang HHP 2004 Quantification of Saccharomyces cerevisiae viability using BacLight Biotechnology Letters 26 989 992 Beckman Coulter Beckman Coulter CyAn Beckman Coulter Inc LIVE DEAD BacLight Viability Kits FUN1 Kf a 135 0063 2 5 7 TOC m 0120 566 730 03 6745 4704 03 5530 2460 bckkcas beckman com http Www beckmancouller co jp2. 10 000 x g 5 HEPES 29 6 D 10umol L Na HEPES pH 7 2 0 2um 1x10 cells mL 5umol L 30 C 30 CyAn ADP 488nm FS Log vs SS Log Figure 2a BacLight SYTO 9 Log FL1 vs PI Log FL3 Figure 1a SYTO 9 Log vs Log Figure 1b FUN1 FUN1 Log FL1 vs Log Figure 2b Figure 1a T 10 FS Log Figure 2a Figure 2b
3. Threshold Threshold 2 FUN1 CIVS 3 SYTO 9 4mL 7096 acquire mode 8 Peak FluoroSphere log
4. BacLight 1 496 Zhang 2004 BacLight FUN1 CyAn ADP 196 BacLight FUN1 5 UL 3 ml 13 ec 4 min 1E seE FUN1 1 Flgure 5 33 000 59 1 947 429 33 000 1 log
5. 630nm 10 000 x g 5 PBS 1 x 10 cells mL BacLight Bacterial Kits PBS 100umol L SYTO 9 600umol L PI 1 1 10 FUN1 488nm 530nm Cylindrical Intra Vacuolar Structures CIVS CIVS CIVS CIVS 530nm 530nm
6. 2 1 LIVE DEAD BacLight Bacterial Kits 2 FUN 1 cell stain Saccharomyces cervisia YPD Broth yeast extract peptone extrose 24 48 25 70C BacLight Bacterial Kits SYTO 9 PI 2 SYTO 9 488nm 498nm PI 488nm 630nm 2 498nm
7. Application Information CyAn No 3 CyAn ADP COE Smart 1999 1 50 000 Saccharomyces cervisiae CyAn ADP
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