1. 30 2 2 5 ml 0 5 1x106 cells ml 2 5 ml well 2 DNA ESCORT Vtransfection reagent 1 3 ESCORT V Transfection Buffer 300ul 300ul ESCORT V Transfection Buffer 3 5ug DNA lt 300ul ESCORT V Transfection Buffer 10 5ul ESCORT V Transfection Reagent DNA ESCORT V reagen 15 20 well 2. 24 lt 72 FLAG 2 G418 4 G418 3 FLAG FLAG 2 Iimma IrEh Co EE ros wed SM A ALORICH 3. b GenElute Endotoxin Free DNA ESCOTE 6 well plate ESCORT V Transfection Reagent ESCORT V Transfection Buffer 1 18 24 2x104 cells cm2 37 C