1.07 In vitro Protein Expression
Contents
1. 2 ml 4 24 GFP2. 5 5 5 Ready to Use 4C 20C 6 DNA DNase RNase GFP GFP pIvex2 3 GFPWT 6 1 ng 2 7 0 5 mm 4 8 co7 1 0 75 ml 2 50 4 5 15 ug DNA 6 DNA
3. T 4 2 RTS 120 180 rpm 30C 24 GFP 30 120 rpm 24 2 GFP 0 5 ml 2 ml 24 4 DD GFP GFP GFP 50
4. 50 pl GFP DNA 0 5 ml 7 ng GFP 3 1 ml 2 1 ml 8 GEP 0 ml 10 ml 2 10 m 7 2
5. Protein Expression in vitro system Osakada Rapid Translation System RTS 00 Roche 1 ml 100 500 uug RTS 500 1 1 E coii 1 0 23 ml 5 2 2 0 8 ml 5 3 3 10 5 ml 5 4 4 0 6 ml
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