KAPA SYBR® FAST qPCRキット
Contents
1. DNA 100 ng RT PCR cDNA RT PCR 10 4 HOX 5 6 8 50I 384 20I ABI 5700 7000 7300 7700 7900HT StepOne StepOnePlus 04uI OgD ABI 7500 ViiA 7 9o4QOgb qPCR PCR Tm PCR 3 PCR Denature 95C 5 sec 40 Anneal extend 60C 30 60 sec Dissociation PCR2. DNA 100 ng HT PCH cDNA RT PCR 1096 4 HOX Stratagene Mx3000P M3005P Ax4000 Hotor Gene V DNA Engine Opticon Opticon 2 Chromo 4 Real Time Detector Mastercyclere ep realplex Smart Cycler Hoche LightCycler 480 Bio Rad CFX96 Ilumina Eco qPCR PCR Tm PCH 3 PCHR jE 1 95C 30sec Enzyme activation DNA 3min Dea g5C 3
3. 20 annealing 72C 10 40 tenti Anneal extend 30 60 sec Dextentian FF Fel PCR 2 Fast qPCR Total 20l 0 KAPA SYBR FASTqPCRMasterMix 2X Universal Feverse Primer 10 M ROX HighLow HOX Stratagene Mx3000P Mx3005P Mx40009 0 4Al Hotor Gene M DNA Engine Opticon M Opticon 2 Chromo 4 Real Time Detector Mastercycler ep realplex Smart Cycler Hoche LightCyclere 480 Bio Rad CFX96 lllumina Eco qPCR 95C 20sec DNA 3min 3 20 annealing Denature 1 3 sec 72C 1 extention Anneal extend 20 sec 3 20 Dissociation PCR Enzyme activation 0sec 3Gmin gt s cenetiss
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5. Tm PCR 3 PCR Enzyme activation 95C 30secD Hold Denature 95C 5 sec 40 Anneal extend 60C 30 60 sec PCR 95C 30sec DNA 3min 3 20 annealing 72C 10 40 extention 2 Fast qPCR Total 20Al 80 KAPA SYBR FASTaPCRMasterMix 2X Universal 10 Primer uM 1 00n Reverse Primer 10M 0 4 00nM CT _ wogo qPCR Denature 1 3 sec Anneal extend 220 sec Dissociation PCR 95C 20sec DNA 3min 3 20 annealing 72C 1 extention
6. 3 20 ABI Takara Stratagene Mx3000P Mx3005P Mx4000 Rotor Gene DNA Engine Opticon Opticon 2 Chromo 4 Real Time Detector Mastercycler ep realplex Smart Cycler Roche LightCycler 480 Bio Rad CFX96 Illumina Eco WW Light Cycler 1 qPCR qPCRH 50 KAPA SYBR FASTqPCRMasterMix 2x Universal DI me MA SUL 400nN Reverse Primer 10uM 400nM KAPA SYBR FASTqPCR dNTP Mixture Mg2 SYBRH Green I primer 0 4 M 0 2 1 0 M 3
7. 95C 30sec DNA 3min 3 20 annealing 72C 10 40 extention 2 Fast qPCR Total 20Al 2g0gl KAPA SYBR FASTqPCRMasterMix 2Xx Universal 10l F rd Primer 4 200nM Reverse Primer 10M 0 4 00nM HOX ABI 5700 7000 7300 7700 7900HTStepOne StepOnePlus 04ZI 102D ABI 7500 ViiA 7 o4aldoab qPCR Denature 95C 1 3 sec 40 Anneal extend 45 68C 20 sec PCR 1 95C 20sec DNA 3min 3 20 annealing 75C 1 extention 3 20 Takara Thermal Cycler Dic
8. KAPA SYBR FAST qPCR X KK4600 KK4601 KK4602 KK4612 qPCR Master Mix 2X O O O O KK4600 1X1ml 100 20 I Rox Reference Dye High 50x QO KK4601 1X5ml 500 20 Rox Reference Dye Low 50X O O KK4602 2X5ml 1 000 20 20C 1 KK4612 30 20 I 2 5mM MgCle KAPA SYBR FAST qPCR DNA SYBR Green1 KAPA SYBR DNA PCR qPCR SN Ct GC AT KAPA SYBR FAST qPCR Master
9. Mix CX MABI GeneAmp 5700 PRISM 7000 7700 7300 7500 RealTime PCR System StepOne M StepOne M Plus Real Time PCR System ViiArM7 Applied Biosystemg Thermal cycler Dice Real Time System TP800 TP850 Stratagene Mx3000P Mx3005P Mx40009 Rotor Gene M DNA Engine Opticon Opticon 2 Chromo 4 Real Time Detector Mastercycler ep realplex Smart Cycler Roche LightCycler 480 Bio Rad CFX96 Illumina Eco Light Cycler KAPA SYBR FAST qPCR KAPA SYBR FAST qPCR ABI ABI GeneAmp 5700 PRISMS 7000 7
10. 700 Applied Biosystems 7300 7500 7500 Fast Real Time PCR System StepOne StepOne Plus Real Time PCR System ViiA W 7 Takara Thermal Cycler Dice Real Time System 3 ABI Takara Stratagene Mx3000P Mx3005P Mx40005 Rotor Gene DNA Engine Opticon Opticon 2 Chromo 4 RealTime Detector Mastercycler ep realplex Smart Cycler Roche LightCycler 480 Bio Rad CFX96 Illumina EcorW eKAPABIOSYSTEAS ABI ABI GeneAmp 5700 PRISM 7000 7700 Applied Biosystems 7300 7500 7500 Fast Real Time PCR System StepOne StepOne Plus Real Time PCR System ViiA W 1 qPCR Total 20 50pl 80 50 KAPA SYBR A RR ESA 6X Universal 5 l Heverse Primer r 10gM 400nM 1 KAPA SYBR FASTqPCR dNTP Mixture Mg2 SYBR Green1 primer 0 4 M 0 2 1 0 M 3
11. ee Real Time System 1 qPCR Total 25ul 2g5 KAPA SYBR FASTqPCRMasterMix X Universal 12 5 Qa ITT1 B Reverse Primer 104M 1og 40onM KAPA SYBR FASTqPCR dNTP Mixture Mg2 SYBRH Green I primer 0 4 M 0 2 1 0 M 3 DNA 100 ng RT PCR cDNA RT PCH 1096 4 25 qPCR PCR
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