リアルタイムPCR（SYBR Green I法）実験ガイド
Contents
1. PCR Ct Ct Ct Ct Tm PCR Tm Tm PCR Tm2. PCR SYBR Green I 1209 96 Sealing Film for 96well Hi Plate for Real Real Time Time NJ500 NJ400 48 24 wellx2 48 48 well snap plate NJ700 Flat cap for snap plate NJ720 PCR SYBR Green 1 QuickPrimer Real me PCR QuickPrimer Real Time MR101 PCR SYBR Premix Ex Tag Th RNaseH Plus RR420A B DNA QuickPrimer Real Time Positive Control DNA MR401 3 1
3. 1 12 2x SYBR Premix Ex Tag 12 5 u1 150 1 5X Primer mix 5 1 601 dHsO 2 5 1 30r 1 1 dH20 5 3 3 Q 5z1 PCR 4 PCR 2 PCR PCR PCR SYBR Green I 1209 4 Ct PCR
4. 2 DNA DNA DNA DNA RNA 2 PCR 2 Thermal Cycler Dice Real Time Thermal Cyeler Dice Real Time Quick Manual PCR SYBR Green I 1209 3 1 3 1 PCR 2 12
5. PCR SYBRY Green SYBR Green 1 PCR R OO MN PCR SYBR Green I 1209 1 SYBR Green SYBRe Green 1 DNA PCR DNA PCR 1 BE D EE 2 FF PP ce F es F 3 kr Vv 0 ee RO
6. PCR PCR 60C 95C PCR Tm Tm PCR GC Primer dimer Raw data lst Derivative re IO I Primer dimer WHR NISNIE Wh EE II HI EIJI RU II EIGHTII IS ESIESIJB II MR Ar ar Raw data 1st Derivative Tm PCR SYBR Green I 1209 2 20 200 1000 1 5 5 0
7. SYBR Green 1 Tm PCR SYBR Green I 1209 Tm PCR SYBR Green I 1209
8. Vortex 1 5 ml 8 0 2 ml PCR Thermal Cycler Dice Real Time System Thermal Cycler Dice 48 Real Time System Lite TP700 Thermal Cycler Dice 96 Real Time System 7 5 TP900 4 SN PCR Thermal Cycler Dice Real Time System 8 96 DD 8 0 2 ml 8 strip tube individual Flat Caps NJ600
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