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ALR-101

1. RE DNA ae PCR Mi DNA Q dUTP DNA CUTP dNTP ILR 10 RE iE TGase DNA rs 1 ALR 103
2. R 10 pM 0 3 1 5 pL 0 2 1 0 pM 40 30 JATP 2 mM 15nL 02 mM ALR 108 dCTP 2 mM 1 5L 02mM dGTP 2 mM 15pL 02mM Stop solution 4 pL TGase dTTP 1 mM 18pL 0 12 mM Q dUTP 1 mM 12uL 008mM IIOIBS325D2 Hybridization Buffer DNA polymerase HI 55C 80 Water to 15 pL J dTTP Q dUTP dTTP QdUTP 60 40 2 PCR PCR Q dUTP Q dUTP 8 PCR PCR ALR
3. 3 PCR i Q dUTP Q dUTP 3 3 lt 3 DNA polymerase KOD Dash LDP 101 TOYOBO KOD Plus KOD 201 TOYOBO Ex Taq RR001A TaKaRa a lr 5 9 10 x Buffer DNA polymerase Buffer Water dATP 2 mM 30 pL PCR dCTP 2 mM 30 LL PCR dGTP 2 mM 30 pL dTTP 1 mM 60 pL MinElute PCR Purification Kit 28004 Qiagen QIAquick PCR Purification Kit 28104 Qiagen 90 MM le PCR 1 DNA polymerase PCR 2 dTTP Q dUTP
4. 1 mM PoR 20 1 PCR PCR PCR 20101203 RN1 8761Rev0 DNA PCR PCR PCR PCR 80 pL QIA quick PCR polymerase Purification kit Qiagen 380 HL 10 x Buffer 15uL 1x n 3 PE FN F 10 nM 0 3 1 5 pL 0 2 1 0 nM
5. 20101203 RN1 8761Rev0 Labelling ONE DNA dUTP dNTP ALR 101 20 C A 1 2 Q DNA 20C Q dUTP dUTP 841 DNA polymerase 6 DNA Q dUTP 20C DNA ALR 103
6. 103 400 ng PCR Q dUTP PCR Q dUTP dTTP Q dUTP 8300 bp PCR Q dUTP KOD Dash KOD Plus TOYOBO TOYOBO 2 4 2 M 100 bp 1 dTTP Q dUTP 100 0 2 dTTP Q dUTP 80 20 3 dTTP Q dUTP 60 40 4 dTTP Q dUTP 40 60 5 dTTP Q dUTP 20 80 Hybridization BufferD9 10 ng mL 55C MM Wash 1 Buffer 2 55C 10 2 Wash 2 Buffer 9 5 2 i 1 Hybridization Buffer 2 M Urea 20 mM phosphate buffer H8 0 0 5 M NaCl 1 mg mL torula yeast RNA 1x Denhardt s solution 1 Casein 0 1 Triton X 100 5 Dextran sulfate MW 500 000 2 Wash 1 Buffer 50 mM phosphate buffer pH8 0 150 mM NaCl 0 1 Triton X 100 3 Wash 2 Buffer 100 mM NaCl 100 mM T
7. ris HC1 pH9 5 50 mM MgCls 0 1 Triton X 100 181 8622 6 22 1 0422 45 5181 http www aloka co jp e mail bio aloka co jp

ALR-101

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