取扱説明書 - Eiken Genome Site
Contents
1. 2 PP 3 F PE 1 LMP541 1 Notomi T et al Nucleic Acids Research 28 No 12 e63 2000 2 Nagamine K et al Clin Chem 47 No 9 1742 1743 2001 3 Mori Y et al Biochem Biophys Res Commun 289 No 1 150 154 2001 4 Tomita N et al Nat Protoc 3 No 5 877 882 2008 5 Kamachi K et al J Clin NASRISI 44 No 5 1899 1902 2006 6 0120 308 4212. EN Eg 6 7 8 1 9 1 RI 2 D 3
3. 3 A 80 E 4 5 4 el E 1
4. 3 4 5 1 6 E DNA DNase 7 E 2 1 20C
5. 8 Loopamp LAMP 2 1 DNA DNA DNA QIAamp DNA Micro Kit QIAGEN 95 C 5 5 p 48 0 6 mLx 1 tube 0 12 mLx 1 tube 60Lx 1 tube 1 0 mLx 1 tube 0 1 mLx 1 tube EE
6. OOOOOOOOOOO R 2 3 1 2 1 20C 2 RM Primer Mix BP PM BP Water DW gt 2xReaction Mix s7DNA Polymerase Distilled 1test gt 10tests gt 2xReaction Mix RM 12 5L 125 L Primer Mix BP PM BP 2 5 L 25L Bst DNA Polymerase 1 0L 10L Distilled Water DW 4 0L 40L 20 0L 200L 1 Loopamp 20 L
7. lt gt 1 2xReaction Mix RM 12 5L Primer Mix BP PM BP 2 5L Bst DNA Polymerase 1 0uL Distilled Water DW 4 0uL 20 0L 4 KEO gt Loopamp 1 20L Lu 5xL LAMP Posi 25D ive Control BP PC BP 5uL Distilled Water DW 5L LAMP gt U 65C 40 80C 5 3 19 Bacto ca
8. 65C 75 C Positive Control BP PC BP Dis illed Water DW LAMP BP 6 40 Positive Control Positive Control BP PC BP
9. 80 5 MS _C DW PC BP 0 6 0 10 20 30 40 Positive Control BP PC BP 2 2 gt RI U 1 5mL 500 uzL LL 15 000rpm 14 000xg 10 U DNA QIAamp DNA Micro Kit QIAGEN 95 5
10. DNA Eiken GENOME SITE URL htt loopamp eiken co jp 1 2xReaction Mix RM 2 Primer Mix BP PM BP 3 Bst DNA Polymerase Bst DNA Polymerase 1 4 Distilled Water DW 5 Positive Control BP PC BP 1 1 RI DNA QIAamp DNA Micro Kit QIAGEN 2mL 20 000Xg 1 5mL 96 100 0 5 mL 1 5 mL 0 5 10 gL 10 100 gL 100 1 000 gL RNase DNase
11. va 20 2 SM 9 2 gszDNA Polymerase 3 4 jp 5 1
12. 5uL 25uL 8 Positive Control BP PC BP Water DW 5xL Distilled 2 1 LAMP 40 80C 5 65C
13. 2 2 2007 143 380115 A
14. 3801 15 A 2011 3 1 A Loopamp Bordetella pertussis Detection Kit LAMP Loop mediated lsothermal Amplification 1 6 4 go 9e7e 2 pertussis DNA 2xReaction Mix CRM Primer Mix BP PM BP Distilled Water DW gsDNA Polymerase gs7 DNA Polymerase
15. samino acids 0 6 NaCl pH 1 x3 4 20 copies test 5 ll 7 1 5 DNA 1 1 LAMP se 2 DNA DNA D DNa
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