HER2検査ガイド−胃癌編− - ロシュ・ダイアグノスティックス
Contents
1. Step 1 Step2 Silver ISH for HER2 RED ISH for Chromosome 17 Red Signal pH Enhancer Naphthol Fast Red Silver A 5 I Silver B Goat anti Rabbit Antibody AP Black Signal e Silver C E Mouse anti DIG Antibody I J Goat anti Rabbit Antibody O Rabbit anti DNP Antibody OH J NN DIG BRL NO Leo 3 CY HER2 DNA Probe 9 3 NO2 Oo H g h 17 S DNP labeled SUO Chr17 DNA Probe DNP labeled Centromere Region d I OOO wu WY CQO HER2 17 DNP HER2 DNA DIG i Chromosome 17 DNA DNP HRP DNP HER2 DNA DNP HRP lg HRP AJ2. 2 40 C 60C 30 3 XT 1 Windows 2
3. START RUN 1 1 100uL 4 2 1 100uL 32 3 lg 1 100uL 8 4 lg HRP 1 100uL 8 5 HRP DAB H202 1 100uL 8 6 DAB H202 COPPER 1 100u
4. SN GS 76 C Exc QUE XT Dual ISH HER2 sent Deparaffinization Cell Conditioning ISH Protease Denaturation Hybridization OC eae a Stringency wash Temperature SISH Multimer SISH Chromogen Red Multimer Red Chromogen Counterstain Post Counterstain
5. XT 1 Windows 2 XT Deparaffinization Sze MUS Cell Conditioning Selected CC1 std Antibody 37C 32 min AB Selected Counterstain Hematoxylin Il 8 min Post Counterstain Bluing Reagent 4min 3 4 5 RUN
6. 10 a 518 PL E SER I VIEW HER2 4B5 HER2 D AQ bs V EM il VP p b ES 2 3 E at E I Ver h a 3 CEDE PU DO QT 4 uy d Q Sy AN Ment P HER2 gt q A x 3 3 TEN 7 we q j lt e js gu B a LA OE 3 P i l P B e Wo gt te CUR E E s af 9 gt a e eva A R Y lt T s ap 5 a LES i v z r o z eor 3 e a e E e EJ amp L a b a ea b j te E amp amp s D LI _ t 4 a E e 2 z lt E on a ex 6 1 A P gt Y e dian ie Ww y Acte Gh L d f T gt es gt Son 4 Ww aD e So d gt 6 e e l ELI p D lt 9 1 e P 5 uc he 555 s b i 2
7. 6 12 e lt sc 1 SIS e e HER2 e 1 5 qo 6 ee 2 8 Chr17 2 HER2 V 1 12 e 4 16 Chr17 2
8. h e HER2 1 e Chr17 1 60X gt WA Do HER2 JT 1 Chr17 e 1 HER2 2 HER2 Chr17 2 4 Chr17 e o 2 HER2 1 HER2 Chr17 2 2 SY Chr17 pi 60X Chr17 E ee 1 e e
9. e s V 2 4 10 20 40 40x Y Y gg unequivocal membrane Score 2 staining visible 10x 20x
10. WI IHC 2 IHC 20 Chr17 HER2 2 0 2 0 1 8 2 2 20 40 HER2 Chr17 yaw 20 HER2 CHR17 CHR17 HER2 20 HER2 Chr17 1 8 2 2 20 20 MM HER2 Chr17 1 2 Chr17 HER2
11. HER2 Chr17 2 0 HER2 Chr17 gt 2 0 Dual ISH HER2 HER2 Chr17 1 2 HER2 Chr17 e 20X 40X 60X HER2 Chr17
12. HER2 HER2 4B5 HER2 C eot un E niche Dl Md De MEI 4 AO e ta 3 E eV e S p o gt tee a a p p T Am ff E Pa gt 2 M Pr adio Dual ISH HER2 Dual ISH HER2 Silver in situ Hybridization SISH Dual Color in situ Hybridization DISH HER2 HER2 17 HER2 17
13. e HER2 2 Chr17 2 BEEN WU TIBI Tl 13 9 2 10 3 e Heterogeneity HER2 HER2 1 Bang YJ Van Cutsem E Feyereislova A et al Irastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2 positive advanced gastric or gastro oesophageal junction cancer ToGA a phase 3 open label randomised controlled trial
14. Z 2View Silver Wash Il 0120 868 555 s XT e 1 FSE e 3 e z gView Silver Wash II 7 gView Silver Wash Il IV 1
15. 40x Score 1 2 5x 5x Y Score 34 2 Score HER2 i don m E RR s R 55 N NE conu mr i a B E EMAIT S GUE EDT DGIO e NEA ler nga gg Hie c ati 2 EB RE RAIA SAN BFR DIEU fc HRS EBLE STE 1096 bl E Equivocal 548 1 E D S
16. HER2 ToGA B018255 ToGA HER2 3 800 HER2 HER2 810 584 5 FU 290 5 FU 294 11 1 13 8 O 74 p 0 0046 IHC 2 FISH IHC 3 16 0
17. B C HER2 DIG AP DIG Chr17 DNA DIG AP lg AP AS TR Naphthol KL Fast Red pH Chr17 I 7 FISH l 132 98 5 Sigel 655i 1 66 1 65 66 66 66 132 I 518109509 HER2 DNA 1 HER2 DNA 10mL 50 HER2 2 Chr17 DNA
18. HER2 B HER2 HER2 IVIEW HER2 4B5 Dual ISH HER2 I VIEW HER2 4B5 1I VIEW HER2 4B5 Ventana Medical Systems HER2 IHC REO I 518107918 HER2 4B5 5mL 50 VIEW DAB 518100032 I VIEW DAB Ade 25mL Ev lg lgG 25mL HRP opens capace es re o 25mL DAB SES 25mL H202
19. 25mL COPPER 25mL e GX e ULTRA s ISH IHC 518103170 MAS GP New Silane 518103033 s HI 518100599 ULTRA 518108922 EZ 518102982 CC1 518103002 CC1 ULTRA 518108939 s II 518102319 518100889 518100605 DS HER2 4 in1
20. 20 6 DIG AP 1 100uL 24 7 AP pH 2 200uL Naphthol 1 100uL Fast Red 2 200uL 8 6 1 40 60C 10 1 30 e L VIEW HER2 4B5 018 P A s DPX
21. Chr 17 ultraView SISH DNP 518109516 ultraView Red ISH DIG 518109523 ultraView SISH DNP x v k 100 DNP o2 mem vc Jp Vee ZOEF 10mL 10mL JI EIO IIA lg A 20mL B 10mL C 10mL DIG We leas op ee 7B anes 10mL APRES A Joni pH 20mL Naphthol AS TR 10mL Fast Red KL 20 mL e GX e ULTRA e ISH IHC 518103170 MAS GP New Silane e e ISH 2 518102272
22. Deparaffinization Cell Conditioning CC2 Cell Conditioning RB Cell Conditioning ISH Protease 2 zs cim ISH Protease 3 ISH Protease Denaturation ABE Hybridization Stringency wash Temperature SISH Multimer SISH Chromogen Red Multimer Red Chromogen Counterstain Hematoxylin Il Post Counterstain Bluing Reagent 4 Cell Conditioning RB Depar Extended Depara Mild 8 16 min Standard 8 16 min Extended 8 16 min 4 32 min 12 32 min 1 12 hour 72 74 76 12 60 min 4 12 min 12 60 min 4 12 min 4 32 min 4 32 min Extended Depara
23. 518107895 E bar II 518103095 II s e e eo ll 1 2 40 C 60 C 30 3 IV
24. Deparaffinization Selected Mild CC2 12 min Standard CC2 12 min Extended CC2 12 min ISH Protease 3 8 min 20 min 6 hours 72C 16 min 4 min 32 min 12 min Hematoxylin I 8 min Bluing Reagent 4min 1 HER2 DNA 2 200uL 6 2 HER2DNA DNP 1 100uL 20 3 DNP HRP 1 100uL 16 4 HRP A 2 200uL B C 1 100uL 4 5 A B C DIG 1 100uL
25. 3 AES A 90 5 b du k A uo ata T H 5 gt Se 8 e 3 4 A t Y t 4 4 J g E g s lt v e IN i gt hcm je 1 d 7 aa a amp E EM us M X x 3 E c E 2 f e Aes 4 a 2 lt o gt i LI a 2 e a p x x is 3 a N i i MI J lt M A k ja w OAS gt ET 4 5 f oak E 2 eS x I v i e af 7 e a 7 Fi e d PF w e M e we Y Y 6 N RM T BD 4 AP t vy w aoc E zd lt r ef X4 AA m sn 8 R m x E lt d zm e ws ww Sy gt 9 wie gt d 2 ah M E A s m s DA as E a p gt A X 2 np lt a P lt e f a aa S 4 E y d p lt lt t xe Da x n E f 1 EE ee f 9 Sf T go dip s ae i x e lt 7 D E Je T Re rua E be ii iae i v d of aa j aos Eum X X ame ae mee Pe ua ONUS ES rA lt AS m M i S i Sex SENA a 2 te X i IFE a i el 7 AN o nd Pn E iy e X Pest
26. ISH 3 518102289 al 518103033 e HI 518100599 ULTRA 518108922 e EZ 518102982 e u traView Silver Wash II 518108892 e SSC 518103026 e CC2 518102999 CC2 ULTRA 518108946 518102319 e 518100889 e HER2 Dual ISH 3 in 1 518109530 s s e e e E bar 518103095 Dual ISH HER2 I HE ORR A s e es
27. Sa Extended Deparaffinization Selected Mild CC2 12 min Standard CC2 12 min Extended CC2 12 min ISH Protease 3 16 min 20 min 6 hours 72C 24 min 8 min 32 min 12 min Hematoxylin Il 8 min Bluing Reagent 4 min EET DeparaffiniZation Cell Conditioning ISH Protease Denaturation Hybridization Ong Is tea a Stringency wash Temperature SISH Multimer SISH Chromogen Red Multimer Red Chromogen Counterstain Post Counterstain 3 4 5 START RUN
28. 2 xt a p ur Lr d Mig Ay te rS P J A n au Kr s AG Per m rt IA p t 4 Ay mat icon 25e 4 ey oo MEUM SUE AM E d 17 OO o eas Og ue gee 5 2 2 gt aepo ACA x OT y NN eS SA e ende X CMS NEU a SS 9 rye beu E Mp gt gt ee w7 re e er Cy A PRD c X AMORE cs i ur P cn i 7 H fg Ip SS ES ms MESS i po ITTF 3 M goa YS CC Ro ab ud CD AN E wA Me ARDEN mai ae hg he Si MV T PR UYS am gt PM Ex i P 7 Up m ow u a _ gt A za M 4 VS NN lt T J EM ed s day I AL v A 7 bi pr ES ry z ak a a rc gt ar N c j lt Q 2x gt e Xx eaten sy A Am 4 E oae aod i A Ay 29 P MY YR Ea ars EVI EAP OFS A Haus v HB r4 A Score 2 e Heterogeneity HER2 PE c e
29. Lancet 2010 Aug 28 376 9742 687 97 2 HER2 2009 9 16 105 0014 2 6 1 http www roche td jp 0120 868 555 Ventana 1014 TD MCI 3000 HER2 01 001A
30. Av VENTANA HER 2 I VIEW HER2 4B5 22100AMX00667000 Dual ISH HER2 22300AMX00002000 HER2 HER2 ToGA B018255 HER2 HER2 2010 1 EU 10 HER2 2011 3 HER2
31. L 4 7 COPPER 6 I VIEW HER2 4B5 B s e 10 2 e 6 48 s s 4um
Download Pdf Manuals
Related Search