T-スポット ® .TB 添付文書第7版 PDF
Contents
1. 8 PBMC A B pH 10 11 3 12. 1 1 A B 1 6 5 10 20 T PHA
3. 121 C 20 1vol1 1 2 8 C 2 18 24 1200 1 Janeway and Travers 1996 Immunobiology 2nd Ed 2 Staines et al 1999 Introducing Immunology 2rd Ed 3 Chapman et al 2002 AIDS 16 17 pp2285 2293 5 5 4 Andersen P et al 2000 Specific immune based diagnosis of tuberculosis Lancet 356 pp1099 1104 5 Behr MA et al 1999 Comparative genomics of BCG vaccines by whole genome DNA microarray Science 284 pp1520 1523 8 8 16 045 473 8887
4. 8 PBS Tween PBS GIBCO D PBS 14040 133 9 10 GIBCO RPMTI1640 11875 093 GIBCO AIM V 31035 025 37 C 11 I 12 13 USB ELISPOT CTL 14 8 Oxford Immunotec
5. A 0 8mLx2 IFN y B 0 8mLx2 PHA 0 8mLx2 T IFN y ELISPOT AP 50nLx1 IFN y T 2 25mLx1 1200 PBMC 8 x600 IFN y A 72mLx1 PBMOC A B 72mLx1 B PHA RW
6. 20 to 1 1 1 5 7 8 5 2 3 ESAT 6 CFP10 BCG
7. M kansasii M szulgai M marinum M gordonae 35 4 5 4Z zezcz os7g 6 1 1 2 A B
8. 1 1 2 8 Cell Xtenco 32 3 18 lt 25 C 4 EDTA 2 1 8 Rs IL 2 0 5U mL 2U mL IL 4 7 5ng7mL 100ng mL IL
9. BCTP 5 NBT 6 7 PBMC TFN y T 1 24 T 8 x12
10. 22400AMT00005000 2015 8 7 2014 11 6 y T TB A ESAT 6 1 2 B CFP10 8 AP y 4 5 4 3
11. 5 1 03 3839 8043 Oxford Immunotec Ltd Oxford Immunotec Ltd 7 Isis Innovation Limited Public Health Research Institute T Cell Xzend Oxford Immunotec Oxford Immunotec Limited Oxford Immumnotec Limited 2015 All rights reserved PI TB JP V7
12. 25 C BD CPT T Cell Xtend RPMI1640 Hicoll Paque PLUS 18 25 C 1000xg 22 Leucosep 15mL Leucosep T Cell Xend 8 PBMC PBMC 15mL RPMTI1640 AIM V 10mL 600xg 7 1mL RPMTI1640 AIM V
13. 15 PBMC Hicoll Paque PLUS Oxford Immunotec Leucosep BD TCPTrw jt 16 Oxford Immunotec T Cell Xtend 8 3 10 6mL x1 8mL CPT x1 4mL CPT x2 2 9 4mL x1 4ml CPT x1 2 2mL x1 18 25 C BD CPT 8mL 1600xg 28 4mL 1800xg 30 18 C
14. T AP SF IFN IFN y 270mLx1 AP IFN y T 2 y 1 5
15. 37 C 5 CO 16 20 15 PBS200nL 4 16 PBS AP 200 50uL 17 AP 50nL 18 2 8 C 1 19 PBS200nrIL 4 20 50nL 21 18 25 C 7 22 23 37 C 4 24
16. 2 3 A B A B 3 3 A B 3 A B 4 5 4 IFN y 1ng mL 2 173 _ 75 1
17. 76 0 97 97 75 98 173 75 76x100 98 7 95 92 9 100 97 97x100 100 95 96 3 100 1727173x100 99 4 95 96 8 100 3 1 80 78 78 80x100 97 5 95 91 3 99 7 2 111 110 110 111x10
18. 0 99 1 95 95 1 100 1 1 2 3 4 2 1 2
19. 5 7 5ng7mL 100ng mL IL 6 7 5ng7mL 100ng mL IL 10 7 5ng7mL 100ng mL IL 12 7 5ng7mL 100ng mL TNEF w 20ng mL 100ng mL IFN w 100ng mL 1000ng7mL JFN 5 100ng mL 1000ng7mL IL TNF 1 1 2 A 3 B 4 PHA 5 AP PBS 200 6 2 5 2 1 2 15mL 3 4 350 1800xg 18 25 C 5 PBMC 6 37 C19C 59 1 COs 7
20. RPMTI1640 AIM V 10mL 350xg 7 0 7mL AIM V 2 5x105 100nL 500nL 12 50nL AIMV A A B B PHA 13 11 100uL 14
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