2 指導上の留意点と授業事例
Contents
1. 2 lt gt NN lt OO2. 2
3. 14 0 0 a 14 15 15 O C 14 1
4. Q 2
5. 1 2 2 1 2 Q 1
6. gt NO 2 80000 90 0 Se 2 1000 1000 0 1
7. 12 5 30 19
8. 3C Ha A MM OH gg2 HO HN PO CH3 NOI 24
9. deciduous trees Q2 A1
10. Or 6 gt trade off merit demerio a lt gt
11. Q 2 2cm 2
12. P 37 38 P 39 41
13. 5 C O O gt O
14. MF Spring ephemeral MM 2 2 4 im FP SO TG 5 6 7 0 5
15. 5 9 _ 41 2 lt gt
16. Camellia japonica 8 8 00 MS 22 2 2 2 6cm 72czz 20 cm gcm 20m
17. Ecology movement Political ecology cost investment benefit Ginitial cost running cost total cost gt 0 02 i merit demerit
18. 3980 g 2 2
19. O 69 Q4 20 a me hl Tc a 20 i 10 25 20 100 100 A
20. P 52 53 2
21. 5 0 6 Ee RN ee
22. 8 gt V2 NH2 8S
23. Q1 A1 7 2 2 2 5 NA
24. 3 EG A SE Nostoc 1 000 Vac tigh PC a X2000 i ee a heterocyst
25. 970 L Margulis gt 1 1 A7 Z
26. Da dalton Da 12 1 12 1 kDa1000Da 1mo1 DE co77 JM109 DNA amp
27. 20 3 TV 4 1
28. i 2 evergreen forest
29. Os 1O SS NR
30. DNA DNA DNA DNA DNA mRNA mRNA DNA DNA 4
31. 65 10 11 12 13 2 10 11 12 13 Rs 23 1 16 7 11 7 6 9 J 3 sn mm hm fh mm 0 el 400 2 4 a ann Tn 3nn 1U nn 1 i gn HU lgnn 1H a 1H 1 1nn 1 100 10 1 10 2HD Imm 1B15g mm 1EHE2 mm 7 19 1 7 12 1 7 12 1 7 19
32. 7 MN 7 Ma
33. 1 hG 3 95
34. 1 2 1 2 1
35. SR CG Re 3 1
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37. 7 7 2 3 50cm Wf Musa basjoo 923cg 15m
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39. 2 4 1 5 60
40. On hg RE CGC CR 2 8 gt 9 0 ey
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42. ROSS MMOIG 0 ER 1993 17 3000 Va 23
43. 5 7 0 2 4 2 14 6 akinete E a A cryptobiosis 2 K St
44. 3 O O
45. AM7 2 8 2 3
46. P 42 44 4 0 1 2 2 1 1 40 100 SRT 1 800 2
47. 3 A 2 18C 15 1 2 1 2 2 Me Q 3
48. P 59 61 7 M _ 58 EE EE EE MY AA NE m HEEL
49. NOV 2 2 2 eo Ed ER 3
50. DNA P 19 20 3 9 4 5 P 20
51. _ 89 1 1 40 100
52. 2 1 22 22 1 Be NN ML A MZ AN Sd 14 ROA NA NEA 1 dia auido A 5 2
53. 0 5 1 2nmg _ 45 3 AG MY
54. Standard Version http www lifescience mext go jp bioethics index html http www lifescience mext go jp bioethics kakusan html http ja wikipedia org wiki E39 839 A99 6B39 829 AF9 B39683968896B39 83 720BC26EJ268226B996E39266296AA26E37268372609A26ES7266926AD26E32683726BS 1 l dR DNA DNA _P dR B 4 P Ry LT gt ED B ele CE eyTG be 8 OH NO 9 O AZ Si
55. 0 3 OE W bacteriophage DNA bacteria phagos CS P DNA
56. A 4 2 a 5 1 2 1 MSH MCH
57. 4 RT 7 7 _ 40 8 2 A 4 7
58. superkingdom 6 1969 R H Whittaker 2 CAT Na TE 2 RS 9 gt YES NO gt YES NO gt gt gt 1980
59. 2Z 20 DNA 1 1 DN A D NA
60. 4 2 Lo 2 3 3 I150 g 4 5 10mL 2 1 NE PAB
61. Ri 9 2 8 9 3 Er 9 0 1 3 4 5 6 7 8 9 10 11 12 3
62. 8 1 750 g 130g 2 3 MRR gg NRT IT 2 _ nn 3 ts wm Ne La 4 a N KN 7 0 Ne H St TA J NY NRU PA 66 g 4
63. 9 2 Standard Version 1 5 MI4 20 1 900 co7 JM109 co7 K12 Green Fluorescent Protein GFP Galactosidase 2 ampicillin 3 DNA 926
64. 7 7 2
65. oc MSH MCH MSH MCH 2 MSH MCH 0 4 4 4 Q 5 MSH MCH A 5 77 47C7 77S7 Y
66. IPTG PTG UV gt RNA GFP POFP Green Fluorescent Protein GFP 4egzo7eg victoria 27kDa 1960 GFP GFP 30 1990 GFP M Chalfie R Y Tsien GFP 2008 M Chalfie R Y Tsien GFP 199
67. UV UV 20 0 OU UV UV
68. 3 9 5mg 2 7zg 9 2nmg f 24 2 5s ee A GE i rp Mi A hi ER V 7
69. 18 3 A B C 15X40 2 5m 1 nl 1 2 12ee 2 2 1 re
70. 1 03 1 2 2 7 1 dQ d c Cy d 2 K
71. 2 MCH MSH A Msn Mon ROD EE 9 n gt gt Ra 2 cn Q 1
72. 2ezergc _receptor 5 al alA a lB og a2 a4A a2B a2C 7 2 2 2M 4843 Catecholamine 0
73. 2 Q A 6 d b RR c W jk 1 EE 9g II f gy 1 h
74. Ao aaane OO Tosnemacwsse OATRRENGS CME a yr Plwdowphemyaanie pOFA cepamomiase oe 1 op 2 a booanae serowese NSR Norepine phnne
75. 4 1 34 2005 9 2 2007 3 100 100 18 40 http nocs myVnc com study uonzujp htm http nocs myvnc com study uonzu htm
76. 2 4 3 4 4 5 6 SOC 7 7 0R IL 10 0 2 0
77. 072 mm 7 193cm 5 9cm Cedrus 6 60 39 0 5 lU Zz 2 2 7
78. 10 130L LB LB amp LB amp LB amp X gal IPTG 11 12 37 24 1 mm I 1 8 1
79. 1938 H Copeland X1932F E Ruska Monera Bacteria 1937 EB Chatton superkingdom 0 96 DT lt gt YES NO x
80. 2 7 5 2
81. 2 DN4 GFP 8 LacZ IPTG GFP LacZ 8 Jsopropyl 8 D 1 thiogalactopyranoside 8 LacZ IPTG X gal 5 1 2 37
82. 4 MR 2 A 4 10c 5L 2g1L 2000 mglL ER A 6 10g lt 5000g X100 Q 2 RB OE KALAC 7 7 7 2 7 g7 C 7g 7 Zs RC ORL KADO3 5 2 5 72gL gg 3 5 mg mg 20000 300 66 6 I67 3 Na el 1 K 86 Ca 1 4 75 a eb cs a FRPEH MNEIRNS 42 3 1 2 PP mg SH
83. LacZ LacY LacA mRNA Io P O Lacl T TacP LacO LacZ LacY LacA 4 RNA pol 2 Pe X P O LacI T acP LacO LacZ acY LacA T RNA pol 0 N 0 CO 0 6 CE IPTG GO gr iE IO CH 8 oH Ho LscH RNA LacZ LacY i 0 AL CHs LacA mRNA OH OH OH Lacl lt IPTG 2 7 GFP
84. CN CG Re 3 V 2 V
85. A 1 2
86. 1 1 5L PAH 90 40 PAH 5mg dL PAH 90 1 1 PAH 18900 mg L X1 5L H 28350 mg 28350 mg 2 PAH MPOPAH 2 28350 ma Hf 0 9 31500 mg H 1 31500 7ze 3 2 PAH 11 PAH 30mg 291500 mg 31500 mg fH X1000 30 1050000 mg fH
87. Ultrafiltration 30cm 1
88. 735 C v Linn phylum division Homo sapiens 19 XR Hooke 665 ns 7 9 is78 E H P A Haeckel LAAT 8 HEE 2 SU AS gt YES NO lt gt Protista YES NO
89. 1978 L Margulis MM 1 RA 1977 C R Woese rRNA 3 1984 Lake Green 1 9 9 0 C R Woese Fiiamentous Myxomycota Spirochetes bacteria Entamoebae Animalia Bacteria Archaea he Methanosarcina ue 2 CR Pa Methanobacterium Halophiles a Eucarya So 0 ML Cyanobacteria Methanoco
90. 4 Ri R gt 49 4 4
91. 8 KJ 100 54 7 6061 PH WW lS
92. 1 a RT CO A AA TaTanaT Tt a Q3 2 7 Ez 0 2 C 3 _
93. 2 3 99 2
94. WS A B plasmid DNA DNA 2 0 kbp ER RAN ES DNA kbp DNA bp base pair 1kbp 1000bp _ 26 LacZ 3kbp
95. C4M Crassulacean Acid Metabolism 4 2 04 389 pm 2010 Cz CaP 2 HM 5 NH4 NOs
96. 2L PET 90 2 1 300 mg L X180 L fH 54000 mg fH 54 g 54g 1 20000 mg L X1 5 LL H 30000 mg Hf 30 gH 92 7 2 g 54 g X100 55 5 I56 26 7 2 2 A 4 1 mg L PAH
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98. 2 1 1 3 2 1 1 Q ee 2 2 3 3 1 9
99. P 23 25 ODNA P 26 28 LacZ GFP 15 97 19 3 30 8 P1l
100. P 29 30 P 31 32 2 24 1 mm 2 5 DNA P1
101. PMMA Rg 2 4 3 6gm 3 10m DN A
102. 2 1 4 6szoc commune blue green algae os7oc blue green algae cyanobacterial 2
103. 29 3 5 3 4 50 L 5 10 4 6 42 1 2 5 7 SOC 260zL 130 L 2 6 8 37C 10 7 9 LB amp 1 X gal IPTG 65
104. 7 Q 2 2 16 2 9170 QG2 8 14 3 18 7 18 2 3 1 C C 19 6
105. 4 ODNA DNA DNA DNA TI mRNI A ODNA 5 70322 5
106. 9 _ 24 VI DNA 2 mRNA M tRNA FIN NN A J 8 0 _ a ee HU NU 5 a 2 VWI DNA DNA 4 15 DNA
107. A D Hershey M Chase 1952 DNA temperate I DNA
108. 8 1 DWHKHE 5 OH 4 Adrenaline Epinephnin CHs Z 7 7 c477 7 _ 5 a gpI amp 2
109. 1 180L 1 10 2L PET 3X X5 30L 3 1 90L 6 3 1 180L 2L PET 1 RR 3 O Q O
110. A 3 7 2 2 18 175 J 2 8 8
111. ec Q 4
112. 1 200 15 1 2 3 4 5 5 T 8 9 10 11 12 _ 9 25 30 1 2 3 4 5 6 7 8 9 10 11 12 5 Kk OBR 7 _66 Z
113. HzS HsO Os gt 27 hd 1 100 0 2 cm
114. 4 13 0 4 2 A T J G CC J GG T 4 J DNA DNA 1 IV 2 10 1 1 gt 2 lt gt H H HOS 9H Ho2e o Deoxyribose Ribose IN So 1
115. LacZ X gal 2 1 IV 7 RM
116. Betula platyphylla gt 2 10m LE 5 86 2 gt Cinnamomum camphora camphora
117. ET EE ET a EEE TI EE FEELEEA MY 59 gt MS 7 25 2 6 2 7 7 mm 2 cz 4escz zy Aesculus hippocastanum marronnier turbinata l 8
118. 1 0 1 1 3 4 5 P 15 18 1
119. 1 1 mm a 2 IPTG X gal 4 C 1 7Z 1 2 250 L 1 2 1 mm 2 Standard Version
120. 100 eee 2 NA 4 9 2 2 EEE 50 um 000005 2 3
121. 1 P 58 61 2 so P 62 71 2
122. i rr Ne 5 0 4 0 60 14Qum 2 5 125um 5 2 LAE EE EN eg 19 A 232 6 nm C 2 7 um SW DNA
123. 2 1 2 1 2 30 a 780 b 0 Th 2 egl 25 5 2 2e 02 ze8 za 2g ssl sg gzg 2z Al zo2 97 102 0 zzgi 105 111 5 171 09 B 7 _47 EH NN
124. 8 X gal 5 bromo 4 chloro 3 indolyl beta D galactoside pTG mRNA BB gt 90 IPTG gt Lacl LacP LacO LacZ LacY LacA P O Lacl T TLacP LacO LacZ LacY LacA T Lacl DNA Lac RNA
125. 6 7 SSHYH9 ee 6 i 8 9 0 0 209 109 0 1 2 3 4 5 6 7 9 1011 12 270 17041 1 2 3 4 5 6 7 8 9 1011 12 1 2 3 4 5 6 7 8 9 10 11 12 5 1 3 3 2
126. 0 5mg http www qlife jp meds rx9696 html SR http Kusuridukuri cho chin com drugs 075hokunalin html _ 46 CaOs 100mL 2 9 A NE ER RE G0 OE TNE A B i tt A B 2 5 A 15C B 15 1 A B 80 2 1 B 2 5 3 80
127. E i sek ND 2 2 3 C A i 5 P2 7 3 2 2 2 KR EEA Nb
128. 1050 L HA 1050 7 4 1 MKFPOMNKMARS A40 60 1050 7 0 6 1750 7 1750 L H 5 1 13 8 7 1 05 kg X V13 4 615 I4 62g 4 6hg 7 2kg7 7 HAS IHNIITGOL OMWKBHRNDL KI8OL OR 5 ORE ML TNS 2 1 HAE A1750 4 4 397 8 2 5 5 44
129. 0 Ampicillin h amp 8
130. 2 ATP 7 DNA DNA 7 DNA H DNA DNA
131. 4 4TP lt lt RuBP carboxylase 5 ecology economics economy 2 E H P A Haeckel 1866 Okologie
132. 9 CE UV UV 7P7C C7P gt 7 7P7C LacZ gt 0 AE TI RE PIG 5 UV UV 7P7C GFP S IPTG
133. IV 1 P 47 P 48 51
134. R 2 1000 1000 120000 a RR NM 1 7 1 5DL 1 1 7 7 7 gL A _ D 4 5 C 10 2 5 FWOK 4 1 5 4 Y X nr OKRRKI LTO 120000mg L 7 9 eg7 120 n 1 HAORR LE lt 7 6 79 L HAH 10007zg 120000mg L 180L
135. ccus T celer Ciliates domain Planctomyces Atoe Flagellates yrodicticu 5 3 s Bact id superkingdom ctophaga Trichomonads Microsporidia eetees CG 202 RE 3 2 MY Diplomonads gt 1938 1969 1978 1977 ri990 5 3
136. r 9 9 GRGELE EE 1 V LE 06 LE HW 9 A Ff LE 08 4 9 EERKSEE 9 EELAGT Ed _ FL on OEE 9 7 3 REI a 0 HAE 68 it EE BB d ECW EOT i EE E ri LM _ fl rs EEFTE LEAYT wii
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