取扱説明書 セルファイン ET クリーン
Contents
1. 10 1 g wet 4 3 3 1 HRE 1 7596 4M NaCl PF mol L mL mL mL 0 00 1 5 0 000 0 50 0 05 1 5 0 025 0 48 0 10 1 5 0 050 0 45 0 20 1 5 0 100 0 40 0 30 1 5 0 150 0 35 0 40 12. 2mL 1 D 10 pH 8 IN027 Ver5 2 April 1 2011
3. 180C3 5cm 230 3 2 ET 209 Ko UK 5 IN027 Ver5 2 April 1 2011 f Cellu in ET IN027 Ver5 2 0 2mol L NaOH 2096 E t OH 0 2molL NaOH 20 E t
4. 0 2mL 1 0 01 0 05mol L 4molL NaCl 100mL 23 4g NaCl 100mL 23 4g NaCl 100mL 2350 3 100mL 250 3 e 7 IN027 Ver5 2 April 1 2011 f Cellu in ET IN027 Ver5 2 280nm
5. 100 8105 2 1 03 3243 6150 FAX 03 3243 6219 E mail cellufine a jnc corp co jp URL http www jnc corp co jp fine en cellufine QoM NaOH 20 9596 3 IN027 VerS 2 April 1 2011 p Celluhine ET IN027 Ver5 2 ET gt ET clean L can easy to remove LPS from buffers ET clean L easily reduces LPS to low concentrations compared with polymyxin immobilized agarose gel or DEAE Cellufine A 500 anion exchanger Materials amp Method Column 9mm I D x 100mm Pump Peristaltic with silicon tube Buffer 1M sodium phosphate pH 7 0 spiked 2 6 EU ml LPS LPS Removal pre washing Column amp media wash with 5CV 0 2 M NaOH let stand for 16 hours then wash with endotoxin free water Silicon tubing wash with 0 5 M NaOH let stand for 16 hours then wash with endotoxin free water 0 2 M NaOH 20 EtOH is more effective for endotoxin free ChromatographyFlow rate 30ml h Residence time 12 8min linear velocity 47cm h Fraction 6 36ml total 128 tubes Assay LAL rate assay
6. 40 509 30 40 HRE Na a 1 ET IN027 VerS 2 1molL NaOH 2 3 4 5 1 2cm 1 2cm 6
7. step 3 step 4 step 5 step 6 S P UD H 1 1 2MPa 2 3 1 IN027 VerS 2 April 1 2011 Celluhine 4 02molL NaOH 2 16 2 5 PF 5
8. pH 10 50mM NaCl X ET ET X ET ET 02MNaOH 2096 ET IN027 VerS 2 ET NaCl 5 1 0
9. NaCl 0 5mol L NaCl pH NaCl NaQl pH pH D L I jo 1A 20 5 Gis 1 jog S 5 os 3 3 05 19 4 5t 02 0 0 9 5 10 15 20 25 30 Elution volume mL B Coke ac LOD Sere Lem eil Fiore nte O SenilL Anis 47an Sampi injectiem Tenil 1 oay tie 1409912 His ink THC pH 3 Goien 0 gt 10 molt Na ET o ET EU ml e BSA mg ml 0 50 100 150 Elution volume ml Removal of the endotoxin from Albumin B BSA Column size LD 1 1cm L 1cm 1mL Flow rate 0 17mL mm 10cmh Sample injection 150mL BSA 1mg mL ET 100EU mL Buffer 50mM PB pH7 0 15mol NaCl 2 IN027 Ver5 2 April
10. Xa CTRL 3 3 2 02mol L NaOH 2096E t 4 C 25 C 16 0 2molL NaOH2096E t OH 3 pH pH pHS8 pH
11. Celluhine ET ET ET or PF PF 20 EtOH EtOH 2 3 1 1
12. EndospecyES 50M Set SEIKAGAKU CORPORATION 10 polymyxin immobilized agarose gel d e e E ii e DEAE Cellufine A500 E AY A A A Se 01 5 oo O d O O O O O O O O OO ET clean L 00I Po DE ee 0 20 40 60 80 100 120 140 F ig Comparison of the LPS removal capability from 1M phosphate buffer of ET clean L and polymyxin immobilized agarose gel and DEAE Cellufine A500 4 IN027 Ver5 2 April 1 2011 f Cellu in ET IN027 Ver5 2 ET gt ET P40 P100 PYREX BE
13. LAL LAL EST 6 ET GH25 ET
14. 1 2011 p Celluhine ET IN027 Ver5 2 1 2molL NaCl UV 02mol L NaOH 2 1 6 2 TOR ET 2096 2 8 C ET L ET L 681 984 324 ET L 681 984 326 ET L 681 984 328 ET L ET S ET S 682 985 324 ET S 682 985 326 ET S 682 985 328 ET S JNC
15. 5 0 200 0 30 0 50 1 5 0 250 0 25 0 60 1 5 0 300 0 20 0 70 1 5 0 350 0 15 0 80 1 5 0 400 0 10 0 90 1 5 0 450 0 05 1 00 1 5 0 500 0 00 NaOH 6 IN027 Ver5 2 April 1 2011 f Cellu in ET IN027 Ver5 2 QRH 10 lt 20mL 0 2g 1 2mL 2 25C QSCSO80AS y DRE 9538 D
16. PYREX BE YE TJVR B 10mL 20mL 1 2 5mL 23CS080AS Y 1 250 3 250C 3
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