FDSS/uCELLアプリケーションカタログ 【リプロセル】
Contents
1. iPSC CM N 9 well N 9 well without heater RT with heater 37 C 50 50 40 40 30 30 20 20 0 0 0min 5min 10min 20min 30min Beating rate bpm 0min 5min 10min 20min 30min Cell iCell Cardiomyocytes Cellular Dynamics International iPS COs FDSS CELL Ca2 30 926 FDSS zCELL iPSC CM Ca22. EE Fig2 t NG oy 2 UMM M Falling Slope NUN MIIMUIRRIMINIMNIME EE 16 OVULATION A 6 em Sepa tees 1 kt bp 7 4 iPS Ca ReproCardio 2 Thawing and Platng cells in 96 well microplate 1 ee Coating lt User s Guide User s Guide URL ReproCardio 2 https www reprocell com wp content uploads 2013 09 RCESD008ReproCardio2 pdf User s Guide RIRRR _ __ Loadin
3. FDSS FDSS CELL 96 384 16 IPSC CM Ca Ca ceo 16 iPSC CM Peak number Total BPM FDSS 2 P P time ms Ave Std Max Min FDSS Ratio Ave Std lt IO Inlmwplahm Ratio AMP RMP RMP
4. iPSC CM FDSS CELL iPS ReproCardio 2 COX SDITIT1 hERG IKr E 4031 Aspirin 0 M 30 nM 100 nM 300 nM 3 pM 10 pM 30 zM BPM P P time AMP 100 1500 4000 80 E 3000 6 oo E 1000 2 E 2000 9 500 20 1000 0 0 0 SN SR NT SN NN SS SS YeYeYs pb 7 4 E 4031 10 nM 30 nM 100 nM 300 nM 10 uM IC50 from BPM BPM P P time AMP 80 4000 5000 60 5 60 3000 a 5 8 3000 40 2000 20 lo 2000 8 20 1000 a 20 0 0 0 conrol 8 7 6 5
5. A10118 24 96ch A10118 26 384ch 96 10 gL 200 gL 384 1 gL 30 L 2 96 ch 384 ch RE 99 B LED ex 470 nm em 540 nm ae NN 4 pe RR 22 EM CCD RE 35 37 0 FDSS PC FDSS SA P 2 0 22 00 FDSS 3 z 7 J 5 i Ca FDSS 10 _ i 96 10 o o 10 550 mm W X 1600 mm H X 670 mm D 200 kg 300 mm W 500 mm H X 500 mm D 20 kg
6. iPSC CM Ca2 100 ms 8 ms L 1 Cell CorAt cardiomyocytes Axiogenesis AG iPS 8 ms 100 ms Ca2 Ca2 FDSS CELL Ca2
7. log home Mi OM LS oY KM 2 NGN SS oS NY o SN 8 KS oS wY oY SN lsoproterenol 0 M 30nM 100 nM 300 nM 10 gM 30 uM EC50 from BPM BPM P P time AMP 90 E 80 E 8 60 Control log fe RO EC FDSS CELL FDSS CELL iPSC CM Ca2 96 384 A11529 01 A11529 03 A11529 04 M11031 01 1 2 96 384
8. ER 1 4 AMP Ave Std RMP nterpolation Ti lm RMP Ave Std a Smoothing _ real iri Lom Slope Ave Std 0 100 10 eg 90 5 20 80 96 30 70 3 Integration Ave Std lrregular peak detection level p PWD PWD10 90 ms Ave Std PBase line me Ca 5 EE TE 1 1 10 20 30 80 90 Peak Parameter Re he post dps 2 ME Fig1 1 2 ya OS ra NN 5 PAUTO PE so
9. iPS 384 FDSS CELL 1 f5 TL pb 7 4 FDSS CELL 3 FDSS CELL iPSC CM FDSS CELL 35 C 37 C iPSC CM
10. mm SZG 1600 FDSS CELL iPS po 3 ReproCELL URL https www reprocell com contact FDSS 2014 1 WWW hamamatsu Com 431 3196 812 TEL 053 431 0150 FAX 053 433 808 1 E Mail sales syS hDK CO jD TEL 022 267 0121 FAX 022 267 0135 TEL 029 848 5080 FAX 089 855 1 135 TEL 03 3436 0491 FAX 03 3433 6997 TEL 053 459 1115 FAX 053 459 1 114 TEL 06 627
11. 1 0441 FAX 06 627 1 0450 SBIS0100J01 TEL 092 482 0390 FAX 098 482 0550 JAN 2014 IP
12. 4F DS 1B 9K2IP 8 lt Ca lt 1 WE 1 PHOTON 15 OUR BUSINESS FDSS7uCELL FDSS CELL 96 384ch 1 96 384 LED 2 4 iPS Ca2 Cell iCell Cardiomyocytes Cellular Dynamics International Probe Fluo 8 AM 96 384
13. g of Ca ion indicator into cells Loading Buffer HEPES Hank s Balanced Life Technologies _ 14025 092 Salt Solution pH7 4 Life Technologlies 15630 056 2 gM Fluo 8 AM AAT Bioqguest 21083 0 05 Pluronic F 127 Life lechnologles P 6866 1 25 mM Probenecid Sigma P8761 Loading Buffer 37 Loadin9 Culture medium 37 C Loading Buffer 80 L well 1 37 _5 CO2 Loading Wash out Loading Buffer 37 C HEPES HBSS 100 L well FDSS Data Acduisiton Data Analysis 30 ON FDSS 10 ms 30 ms EE 4 iPS
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