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1. HPLC 09 20 39 EY on Eee SMC Press AD LC MS Analyst Xcalibur rrent6 4MPa Drift0 12MPa 1min 52. 10 LC Caf6 No 14 rt ctime oc ats a daca i SAG BEAR a oa a a le lt Ouse LC cafe No 13 NANOSPACE NOANOSPACE NANDSPACE 1 1 Real LC cafe LC cafe No 14 T 2 ria HIUEIDO 105 0021 1 1 16 FS TEL 03 6253 1412 FAX 03 6253 1416 601 8037 18 TEL
3. GLP EN GLP EZChrom Xcalibur Analyst 1265312 42 2 1265312 42 2 A6000 21 6 46000 21 6 0 0 RR RR RIRLILLS MS NANOSPACE LC MS MS SMRM Creator tSHM Creator Analyst 1 5 AB SCIEX Xcaliburg 1 Thermo Fisher Scientific Scheduled MRM Timed S
4. Nucleonavi Nucleonavi 7 800 a 8 45 1 6 x 150 Gig 18 nm 34m 9 0 MPa 100 Ca 30nm 5 um 2 5 MPa AAMUMML 50 C18 30nm 5 um 3 2 MPa 0 Nucleonavi 8 6 MPa mAU T T r T Da 4 6 8 10 18 14 16 18 80 88 84 minutes HPLO Bl 100 L min 8 3 Nucleonavi 60 so Cg 30nm5m 18 4 MPa JJ AUUUUA Si Cig 80nm 5 m 14 7 MPa Nucleonavi 12 7 MPa 10 T T T r T T T T T 8 10 12 14 16 18 ao 28 24 minutes HPLC 3
5. 10 Tet Mo ovedeo cam 6 a 158 mi Method sa tSRM Creator csv Method AM IN Sheeto NANOSPAOE i mm ep we EE we Analyst Ele ISO He lt COTE EE sw Edter oetwhe Tne Mattod etod Summey Eh Rn Setines NS Acqure Time mn Fa00 Ewaiment pe RM Chrom Eer fkwame FD Colision Qas Pressure Te RO ee ipeukwWahFwHham Dee Taal EGO ShaoaMr lm Stedo S12 urent protuer gnion norey Sle Soe Pan Mame Term wo ro rsi rom mam TGs TO4609 0 om NO ar BIT semoT T2000 omo gm te ET Tog7 Teg7 HT TER 0 se ag ns ier 996 549 1439 Asoetg XCalibUr sts 35ooo te ae reset Method Bkg stat Pos from RT sec IO Bkg width sec 20 Em RT window eol RT view width min F500 smootingWidh RB Noise Percent Peak Split
6. 8 400 L min EE 4 Diversity of Mass Spectrometry 82010 7 7 9 amp 7 8 NANOSPACE NASCA MS 7 13 7 16
7. Keywords NANOSPACE NASCA Carryover LVFX NANOSPACE NASCA Straight Injection Technology NANOSPACE NASCA 80 80 LVFX 3 0 ug on column Enlarged xa20 1 LVFX 3 0g n 3 OH Levofloxacin Mol Wt 361 37 LVFX 1 3min not detected LVFX 3 g LVFX3 ug LVEX3 Lg 3
8. 075 671 0301 FAX 075 671 0302 http www shiseido co jp HPLC le Kcaleleler on oy4
9. HPLC CAPCELL PAK Cs MGII S83 2 0 mm i d x50mm A 0 1 vol HCOOH B CHaCN A B 85 15 200 gL min 40C PDA 292 nm 5 AL Sample dissolved in Mobile phase Blank Mobile phase T T RE T T 0 4 6 0 8 10 18 14 1 6 1 8 Minutes Minutes PDA 298nm LVFX Nucleonavi Glass Clad R Column Keywords Nucleonavi Nucleonavi 1 0 mm i d x 50 mm DNAdT12 18mer 50L min 8 400 L min 1 8 50L min 400 L min
10. NANOSPACE NASCA sMRM creator E 1 AS e le 2010 7 81 7 2 7 LC MS NANOSPACE NASCA LC MS Nucleonavi 12 RNA No RNA No Life 82010 7 87 89 NucleonaVi 2010 11 26 HPLC
11. a b 1 9 1 3 22 225 200 175 150 185 50 L min 1 0 MPa 3 E 1oo 5 100 L min 8 4 MPa 50 200 L min 5 5 MPa 800 L min 8 8 MPe EG NO 400 L min 18 4 MPa avb 3 28 0 2 4 6 8 10 12 14 16 18 20 22 24 minutes 1 Nucleonavi DNA12 18 mer HPLC Nucleonavi 1 0 mm i d x 50 mm A 15 mmol L TEA 400 mmol L HFIP CHaOH 95 5 B 15 mmol L TEA 400 mmol L HFIP CHsOH 50 50 B 0 0 min 3096 15 min Nucleonavi 1 0mmx250mm 5 m 3 m Nucleonavi 9 2 Crg8 Cg Nucleonavi 30 nm Cg C1a 3 m C18
12. HPLC 7 CONTENTS hy75 NANOSPACE NASCA Carryover INIITa 3gIEN4 EE IgE selgglili Real LC caf amp HLEIDO sov7hdzzseea5l HPLC LC M8S HPLC HPLC
13. RM 100 sMRM Creator tSHM Creator LC MS MRAM Wil Data WnwwtDgaPiqeckNSJNRMNDget20030417NSMRMwii mwAcdede imDamiedGGTIWIVcaenfefen7TRTOL e TIC SC EE TIG of MRM G7 paie tem sample2 GO0petnL soe 29 tSRM Creator psa MRM Same 22 MI EL COT _ SsMRM Creator tSRM Creator s
14. ing Factor EF Base Sub Window min I Report Largest Peak Save as e Method 100 SMRM Creator tSRM Creator NANOSPACE 3820 SMRM Creator Y200 000 Analyst1 5 3830 tSRM Creator Xcalibur 1 HPLC LGC MS OHPLC LC MS 9 NANOSPACE NASCA Carryover
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