事例Ⅱ 遺伝情報とDNA
Contents
1. Da dalton Da 12 C 1 12 1 kDa 1000Da 1mo1 282. No ar NAM 81 i 9 1 2 5 1 4 20 coli K12 3
3. UV UV 7P7C GFP HS IPTG LacZ X gal 32
4. P11 EN 1 2 1 3
5. 25 bacteriophage DNA bacteria phagos Ss P DNA A D Hershey M Chase 1952 DNA temperate
6. DNA lllumumuumJm DUNA
7. co7i JM109 DNA amp 2 DN4 GFP 8 LacZ IPTG GFP LacZ Jsopropyl 8 D 1 thiogalactopyranoside 8 LacZ
8. P 31 32 15 97 19 3 30 8 P1 2 24 1 mm 2 5 DNA
9. LacZ 3kbp X gal 5 bromo 4 chloro 3 indolyLbeta D galactoside IPTG cz gt 4 A IN 2 m 1 amp pUC19 X gal IPTG gt Lacl LacP LacO LacZ LacY LacA P O Lacl T LacP LacO LacZ LacY LacA T Lacl
10. Y tRNA RS m DNA 4 4 15 DNA 0 3
11. 1 2 4 3 4 4 6 SOC 7 i O1 30 IT 10
12. 260rL 130rLX2 6 8 37 10 7 dt RN 6 I i i 65L 10 0 130L 11 12 37 24 1 mm I 1 8
13. 1 2 1 mm 2 Standard Version 29 3 5 3 4 50gL 5 a 10 4 6 42C 1 2 RN 3 7 SOC
14. 1 UV UV
15. IPTG X gal 8 1 2 37 1 1 mm 2 IPTG X gal 4 1 7Z 1 2 250 L
16. UV UV gt 31 UV UV 7P7C GC P 7P7C 72cZ gt IPTG X gal 1 5s
17. DNA ODNA SR 2 4 Standard Version http www lifescience mext go jp bioethics index html http www lifescience mext go jp bioethics kakusan html http ja wikipe
18. 1 I DNA DNA D NA P 23 25 ODNA P 26 28 LacZ GFP P 29 30
19. Green Fluorescent Protein GFP E ampicillin Standard Version 3 coli JM109 14 800 Galactosidase 2 E 1 E 6 ODNA DNA PO A DNA
20. DNA Lac RNA LacZ LacY LacA mRNA 1 V P O LacI T LacP Lac0 LacZ LacY LacA T X RNA pol Uv OE a A a a X P O LacI T LacP Lac0 LacZ LacY LacA T 1 RNA pol UN IPTG CHO CH2OH GHzOH CHa HO 5 oH HO so RRNA LacZ LacY oH oH on a LacA mRNA 1
21. G T 4 DNA DNA 9 IV 2 10 1 1 lt gt lt gt P MI 0 NT NN_iM ME gt 8 Deoxyribose Ribose SIT 2 24 VI 2 0 2 DNA so a DNA 6 iii mRNA
22. 1 i Lac IPTG 7 GFP RN UV gt AV4 GFP pm a GFP pGFP Green Fluorescent Protein GFP 4egzo7eg victoria 27kDa 1960 GFP GFP 30 1990 GFP M Chalfie R Y Tsien GFP 2008 M Chalfie R Y T
23. Fe A B A plasmid DNA DNA 2 kbp DNA DNA DNA kbp DNA bp base pair 1kbp 1000bp 26
24. dia org wiki E3 83 A9 E3 82 AF E3 83 88 E3 83 9 BC E3 829 B9 EB3968296AA E39683969A B39839 AD B39839 B3 DNA I DNA _P dR B 4 P JR 9 PR B em Le 3 TIT 3 oH Ho ge 0 ON DD jC2 H HzO amp 4 2 A T J G C1
25. sien GFP 1990 Ampicillin amp 8
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