MultiColor-LucTM Reporter Assay Kit
Contents
1. AN 0 0 0 00 0 0220 00 00 0 00 0 0 00 00 00 0000 0 0 00 0000 000 i 2 0000 0000000000000 00 0 00 0000 0 206000061 000 O UO 00 OMultiColor Reporter Assay O 0 0 0 100 00 00 000 002 30 000 00 0000 0 00 0 00 0 0 0 0000 00 0 0 0 00 000 00 000000000000 0 0 000 0 30 1100 0 0001 OFF OOOO DO 00 00 00 0 ZAT B ay yT J B H pi ji ii aii PT 2010910 0 0 00 00 00 0 0 00 0000 0 A ELE 000 000 0 00 00 00 0000 000 0 0 0 0 00 00 000 00000 000 SN 00 0 0 0 0 0000000 00 00 00000 109000 00 F2 00 0 0000 000 7200 000 00 0 000 OOO 00 0 0 000 00 00 00 U 000 0000 0000 20000 00 0 0 000 0 000 29 500 0002. 000 00 0000 0 MEAD 0000 0000 00000 2 20000 X II 00 U 2014400 0 0000 00 U 00000 0 0 0 020 000 000 UU 0 0 0 0 0 0 00000 JL Analysis O0 0000 00 5000 00 0 0 00030 LILIL Integral 5 0 50 Ul Integral 0 0 00000 50000000600 TI 101 30 0 0 000 000 0 000 000 000 0 0 0 000 l UUUUUUUUU UU O OOOO UO HU 0000 UU U U Luc Reporter Vecto 0 0000 0000 00 000 0 00 0000 00 0 0 0 00 00 00 00000 0000 0 010 0 00 0 00 MultiColor Luc Reporter Assay 00000 10010000 000000000000 000 0 00 0 0030 OOOOO 000 000 00 00000 0 0 0000 00 00 0 0000 00 5000 0000004 00000 0 00 15 7 00 3 Tj 00 0000000000000000000000000 00000 0000000000000 EE 0 0 00 0 0 0 00 00 000 A1 000000000000000000 000000000 0039 3 00000 000 0 00 00 00 000000 000 00 0 00 0 220 0 0 0 00 0 0 0 000 00 00 0 0 00 00 00000 0000 204 0000 000 O 00 OMultiColor Luc Reporter Assay O 0 0 U 100 1 201 000 00 000 00000000 00000000
3. 0000 I 0 00 000 0 0 0 0 00 0000 U 000 VII MultiColor Luc Reporter Assay i 1 000006080 JUL MultiColor Reporter Assay 0 A 00 0 0000 0 0 0 00 000 00000000000000000 00000000000 00000 0 0000000 00000 000 0000 000 0 0 0 00000 0 i 2 00000000 Reporter Assay KID 000 0 00000000 5000 00000 0 000 00000 02 20 00 0 0 0 0 0 00000001000 i 1 00000000 JUL MultiColor Control Vector pSLG 5 40 control pSLO 5 40 control pSLR 5 40 0 00 00 0 0 00 0 0 0 00 00 00 000 00 0000 0020 0000 00 UMultiColor Reporter Assay 00000 10011000 00000 000 000 00 0 0000 0 0 0 0 0 0 0 0 00 00 000 0000 00 0002 a Di mi 00 00 00 L 21
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5. Y Z Y X 2 X RED 564 701 537 608 387 135 0 952 0 686 OR 5 932 734 4 334 701 1 461 892 0 731 0 246 GR 15 359 496 5 658 781 1 232 040 0 368 0 080 24well 00 00000 00 CHO K10 0 pSLR O G 5 40 0 0 0000 0000 10 00240 00 00 0000 000 0 00 000 0000 00 000000000 0000 0000 000 03000 00000 0000 0000 300 O UULU 000 1 Multicolor 0000000 a Filter 0000000000 08000 000000 200 0000 00 F1 F2 b Step Time 0000 0000 0 00000 1sec c Auto Calc 00000 0 000 2 Photon 00000000 00000000 OFF 3 P Int 000000000 0 0000 00 M uL 4 Analysis Tine 000 00000000 20 35sec 5 Result Formet 0000000 0000000000000000 00 Intgral 6 Integral Start 0000000 35sec 7 Print Kinetic OOO0OO0OOODOOCOOOOO0O0O 00000000000 00 OFF 0 0 0 00 000 F2 UU U UU U UGRL ORL F1 O56 22260 OR RED 2 6 21 000 00 00 0 0 0 0200 00 0 00 0 00000 000 0 0 0 0 0 00 0 0 0 00 00 0 0 OU U 00 0 0000 00 0 00 00 0000 L 0000 00 0 0 l 25 C UU 120 120 100 i 100 2 2
6. 1 O 1 Opn o g aga 44 O 9 go O koog O 00000 UU 12 00 00 0 00000 0 0 30 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 00 00 l 0 O 0 00 000 O O 0000 L 0000 00 ELLE 000000000 0000 00 0 0 00000 FL sod O 000 L 13 XI U 00 0000000 000000000000000000 0000000 C BD ________ 5 600 000000 900000 _ 000 Mev DD 07 e ES ultiColor Vector Set pSLG test pSLO test IpSLR test Vector ultiColor Luc Control Vector Set MCL VS2 pSLG SV40 Control pSLO SV40 Corntrol pSLR SV40 Control Vector MCL VS1 ultiColor Luc Vector Full Set pSLG test pSLO pSLR pSLG 5 40 Control pSLO SV40 Control pSLR SV40 Control OSLG
7. OL 00000000 MultiColor Luc Reporter Assay 030 00 0000 0000000000 0000 00000000600 0 0000 0000 0 0 4000 800 O 000 4501 750 nmi 000000030000000000 00000000000 000000000000000000000 00 00000 00 00 0 0 00000 00000000 0000 3000 000000 0 MCA 0000 0 0 0 U 00 0000 0 00 00000 0 2250000 00 000 00 000 0 000 U 10910 Luc Reporter Assay System U E 000 0 0 0 0 0 00000 0 0 0 0 000 00000 13 00 0560 ni 000 0000 00 0 600 0000 00 0 U 0000 E UU 00 0000 0 MCAD UULUUUUUUUUUUU 00 030 0 0 0 0000 0 URED 1 0 0 00 U U O00 000000000000 0 OU 00 100 0000000 1 000 00 200 0000000 F2 9 000060 REDO 000 00 00O Tir FII GRO 00 F1II I II Tig REDO 000 520 000 Tar 0000 GRO 000 2
8. 000 00 0000 0 MCA 00000 0000000001 00 000 00 00 00 0000000 III 0 0 00 0 0 0000 0000 00000 3450 0 0 0003 n uMitiColor Luc Reporter Assay 0 0000000000000 0 00000 000 0 000 009 0000000000 0000 UU 1 000000000000000 L 00000 00 000 uL 5 961 0000 25 0 1 480 65 0 3 24 1 00 0000 100 0 5 120 00 00060 200 1 60 0U OUL L 500 2 X LIU UJ UO 41000 U U v 000010 MultiColor Luc Reporter Assay Kt H 0000000000 0 00 0000 000000000 0000 000 00 0000 0 00000 0000 00 0 000 0 0 000 00 0 000 0 0 UO U 0000000000 0 0 00000 0 000 00 0 0 I 00 000 00 0 000 U 0000 000 0 00000 VII 30 00000000L UU 08000 0000 000 2000000 00000 0000000 000 00 00 80 0 0 0000 000 x 0 0240 00 0 0
9. 80 e 80 9 e RED 9 RED p 4 OR 0 0 3 6 9 120 0 3 6 9 120 Time min Time min 0 4 00 0 00000 000 000 0000 0020000060 00000 00 000 0 00 0 0 0 1090 2000 0 0 0 0 00000 25 000 000 200 00000 5 00000 125 0 0 0 0 0 0000 20000000 REDO 0 025 20 00000 4000 CRO 00 00 0000 0 0000 0 L 5 0000 11 000000000000 4 g gono m oot oo E H LIF O r O Ola 99 o 2 ho 2 ie 7 H 2 o 00 agg O g og 0 J o o A t I oom pagoa O 1 5 o 55 P Oaa 20 ja DpH go o a 1 24 7 1 50 H z goH 2 lt 6 Moot Ei 19 A 1 1
10. Reporter Assay 00000 00 0 0 0000 00000 0000 0000 Reporter Assay 00 00 00000 000 2000 00 000000000 00 00 0000000 00000 00 0 0 0 O0MultiColor Luc Reporter 00 2004 33345700 0 0000 1 00 ______000 ____ ___ 00 __ 0000 _ 10 nix 10 MultiColor Luc Reporter Assay Kit __ 200 809000 30 10 Luc Reporter Assay Lysis Buffer Il 0 Luc Reporter Assay 80 0 0 00 20 4100 0000 005 0000000 00 0 SOC 0 000 0 00000 UUUUUUUUUUUU U 299 00 00000 000 0 00 0 00 L 0 I 00 0 000 2000 000 000 000 0 00 0 020 00 0 0 0 0 000 00 00000 0 0 0 0 l 000000 0 000 00 00000 00 0 000 00 0 0005 0 0 000 00 FL 5000 0 00000 00 00 0 000 0 000 000 0000 00 0 0 00 00000 Luc Reporter Assay MultiColor Luc Report
11. 00 000 00 000000000000 0 00730 0 0 0 A1 000000000 00000 000 00000 0 0 00 0000 00000 0 0 0 0 000 000 00 s R 00 0 0 000 0 00 29010910000 00 00 00 0 00 00000 30 0000000 000 0000 0 0060 0 0 00 0 0000 000000000000 00000000 00000 L XO 2000000000000 1000000 00000 0000000 000 00 10 80 0 0 0000 000 x 0 0240 00 0 000 00 0000 00000 000 III 00 0000 0 MCAT 0000 UD 0 00000 0 2 00000 00 00 0 0 0000 00 U 00000 00 0 OGR OR F1 GRA RED 220 10000 000 00 0 0 0 0 0 0 0000 000 0 0 00 0000 0 00000 0 500 0 00 0 0 0 00430 Integral 5 50 OM ntega 0 00000 5000 000 000 TT 0 00 000 000000000 10 000 000 0000 0 0 000 0 00 00006 HU 0000 00 0 Reporter 000 0 0000 00000 0 00 00 0 0 00 0 0 0 00 00 0 000 00 0000
12. 0 T2 RED 0000000000 ROOUGO 00 00000000 000 00 0 0 0 00 0 0 0000 000 0000 000 000 0000 0 FO R G O F1 T1r R T1o O T1g G 22 T2r R T2o O T2g G UU URED OR GRO 0 0000 0 0 000 ROUCO 00 UU 000 00 0 0 6 0 000 U 0000 00000 _ Tlg T2o 2 20 120 10 2 T1gT20A 1r T29 1o T2r 1 2 20 10 29 Tlr T2og 2 2 2 1 20 10 T2r T1g T2r T1r T2o 2 9 2 2 20 2 1 1 2 T1r T2o Tlo T2r T1g T2r 20 0 0000 0 2004 3334570 000000 00000 000 00 00 000 000 00 0 00000 U 00000 0 0000 000 000 00 0 0 0 0000 0 0 00 0600 00 000 000 0 0000 0 0000 0 00 0 0 0 0 0 0000 0060 0000 0000 0 06 000000000000 L MultiColor Luc Reporter Assay 00 0 00 0 0 00000 0 0 00 0 0 00 0 00000 00000 00 0 0 000000 0000 0
13. 0 0000 070 000000000000 000 000 0 0 00000 2000 0000000 0000000 0000 0 0 000 I 0 000 0 0 0 0 0 0 0 000 00000 00000 0 0000 0 0000 00 0 III 0000 00000 00000 1 100 II I N 00000 0 0000 000 Helal D NIH3T30 CHO K10 0 4000 35000 30000 z 3000 25000 9 o 20000 2 15000 lt 1000 5 10000 5000 0 0 RED OR RED OR GR RED OR GR 0000000 0000000 000 00 DNA II IJ JI IILI L 24well 1 00 00000 00 Hela CHO K10 00000000000 0O 000 pSLR O G Ssv400 0 0 0000 000000002410 00000 0000 0000 000 00 DNA 00 000 00 0 0 00 00 1000000 000000 0000000000 UXO 0 10 O D 20 000 0 2210 000000 00000 10 000 00 0 0 00000 0 00000 000 0 0 00 0 0 0 0000 0 0680 00 0 00 0000 0 0 00000 0 0000 0 0 00000 2009 0000 000000 00000000000 p 100 O U 2 0000000000000000 0000 0 000000 00 0 02 00000 00 0000000 0000 0 00060 0 0 0000 0 0000000 000 000 0 000000 000000 0 0000000000000 0 J IJ IBD 0000000 0
14. 0 010 000 0 00 MultiColor Luc Reporter Assay 00000 10010 000 0030 00 000 000 0 00000 0 0 0000 00 00 0 0000 00 5000 000 00074 00000 0 00 15 7 0 0 0 0 0 000 000 0 00 0 0 00 0 0 0 00 00 000 Al 000000000000000000 000000000 0039 00000 0000 0010 0000 00 0 0 00 0 0 00 000000 000 00 0 00 0 220 0 0 0 00 0 0 0 000 00 00 0 0 00 00 00000 0000 204 0000 000 O 00 OMultiColor Luc Reporter Assay O 0 0 U 100 1 201 000 00 000 00000000 0000 00 00 0 00 000 0 30 Al 000000000 00000 000 000 0 00 lI UI 00 00 00 00 00 0 9000000001 0 0000 00 29109010000 00 00 00 0 00 00000 I 00000000 00 0000 0 0060 0 0 00 0 0000 000000000000 00000000 00000 L 10 O O ODOL 2 UUUUUUUUUUUU 0000 00000 056 O00 00 860 F1000 F2 FO count F1 count F2 count T1 T2
15. HSV Control Vector 10 MCL VS3 000 00 00000 0000 000 0000000 ____ Dm 00 MCL C11 ultiColor Luc SLO TA Cloning Kit MCL C12 Luc SLR TA Cloning 000000 0000000 Bu 00 SLGOR F primer 0000 0 0005 90 5101 5180 O 0 SLGO R primer 00000000000 000 0 0 0051670500 0 200 prols SLR R primer 00000000000 O00 0 0 0 00 0 JODO DDDOD I O O O D N 14 l LI hhi titi E 9 000 17130 0 104 0031 00000010 2313 TEL 03 3292 1954 03 3272 8276 E bio toyo b net co ww tovyo b net co 15
16. TOYO INK MultiColor Reporter Assay Kit OUUU L I VI VII VIII IX XI XI XIII 0 Luc Reporter Assay O O O Luc Reporter Assay System O MultiColor Luc Reporter Assay 00000 000 O OOOO 000 0 0 LU Q N L 2005 8 0 MultiColor Luc Reporter Assay Systemi I 0 0 0 00 0 00 0 0 000 0 0 0 0 0 0 0 0 0 0 0 0 00 00 0 0 0 0000 0 8000 0 00 00 01 0 00 0 0000 0000 00006 0060 000 0600 0000 000 00 000 0 0 0 0 0 0 00 0 00 000 00 U 0 0 0 0000 e 00 0 0 0 00 ENE 00 0 0 0 0 0 00 MultiColor Reporter Assay Syster 00 00 00 0000000000 0 00 IREDI CORO 0 00000 000 00210 00000000000 00000000 0 0 0 0000 0000 00 0 00000 0000 00000 0000000000 0000 2000 00000 0000 0000000 000000000 00000000000 0000 000000 0000 0 ILILMultiColor
17. er Assay 00 00 0000 00000000 30 0 U LAO 00 0 0 0000 00 0000 CO MTH 200000 0000 0 00 00 00 0 0 0 0 0000 00 0 0000 12 GR RED 1 Relative intensity O 400 500 600 700 Wavelength nm 1 00 00 0 0000 000 000 00 00 0 L 000000 0000 00 0 000 00 000 0000 0 00000 LE 0 0000 0 00000000 0000 00000 A 0 0 00000 0 000 000 L H 3 Luciferases O N N y ATP O gt f AMP PPi CO HO S S HO S S D Luciferin Oxyluciferin Red light rrax 630 nm Orange light max 580 Green light max 550 nm 0 2 000 0 0000070 0000000000000 0000 IU UU 00000 000000 000 pyralis 0O O 0O 0000 III III UU 000 0 O O OOO U U U U U A max 630 nm mex 0000000000 0 0000 00000 Rhagophthalmus 580 000 00000000000 00 0000060
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