取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. 1 MultiReporter Assay System Tripluc nm SLG 550 SLO 580 1226047 SLR 630 90 SLG Green Light A max 550 nm SLO Orange Light Amax 580 nm SLR IT ATP O Oxyluciferin AMP PPi CO Mg D luciferin SLG SLO SLR T T T T 450 500 550 600 650 700 750 nm Red Light Amax 630 nm 2 SLG SLO SLR 2 MultiReporter Assay System Tripluc MCA Code No AB 2250 Phelios Code No AB 2350 ARVO2. 3 1 450 750 nm 2 2 3 4 1 80 C 2 96 1 MultiReporter Assay System Tripluc
3. 1 2 3 MCA Phelios 560 nm 560 nm LP Filter 1 600 nm 600 nm LP Filter 2 EF SLG SLO SLR 560 nm LP Filter 1 600 nm LP Filter 2 SLG Filter 1 Tig SLG Filter 1 Filter 2 T29 SLG Filter 2 SLO Filter 1 3538 XE Tio SLO Filter 1 Filter 2 538 T20 SLO Filter 2 SLR Filter 1 Tir SLR Filter 1 Filter 2 538
4. 50 8 1 Nakajima Y Kimura T Sugata K Enomoto T Asakawa A Kubota H Ikeda M and Ohmiya Y 2005 BioTechniques 38 891 894 2 2005
5. TNFa PMA DMEM 10 FBS 100ul 37 5 G O R DMEM 10 FBS No o 1 5 2 1nM PMA 6 0 001ng ml TNFa 3 10nM PMA 7 0 01ng ml TNFa 4 100nM PMA 8 0 1ng ml TNFa 9 o 1ng ml TNFa 10ng ml TNFa 4 CO Tripluc Luciferase Assay Reagent 10 ARVOwx 660nm 100nm Filter3 595nm 60nm Filter2 510nm 60nm Filter1 2sec G O R 5 2 2 p 7 G O R
6. SLG SLR SLO SLR 600 nm LP Filter 2 SLG SLO 560 nm LP Filter 1 6 2 3 ARVO 510nm 60nm Filter1 595nm 60nm Filter2 660nm 100nm Filter3 SLG SLO SLR Filter 1 Filter 2 Filter 3 Ri SLG Filter 1 Tig SLG Filter Filter 2 T29 SLG Filter 2 Filter 3 T3g SLG Filter 3 SLO Filter 1 Tio SLO Filter 1 Filter 2 538 T20 S
7. 3 x1 MMULT 3 x3 3 x1 2 Ctrl Shift OK Microsoft Excel 6 MultiReporter Assay System Tripluc AP1 NFkB 1 pSLO test Code No MRV 102 pSLR test Code No MRV 103 HSVtk AP1 5 ATGAGTCAA 3 6 NFkB 5 CGGAAAGTCCCA 3 6 pAP1 SLO pNFkB SLR 2 96 Code No LS 136101 HeLa S3 1 2x10 cells 100yl DMEM 10 FBS
8. 96 100 11 100 4 10 10 5 5 MultiReporter Assay System Tripluc 1 MultiReporter Assay System Tripluc SLG SLO SLR 3 1 SLG SLO SLR
9. Es lt gt lt Tripluc Luciferase Assay EM Reagent 1 Tripluc 2 Code No Mun poner Assay System Tripluc 10ml MRA 301 80 C Tripluc Luciferase Assay Reagent Moi poner Assay System Tripluc 10mlx5 MRA 301X5 80 C Tripluc Luciferase Assay Reagent 96 MRA 301 100 MRA 301X5 500 3 3 3 MultiReporter Assay System Tripluc MultiReporter Assay System Tripluc 9
10. 1 2 3 SLG SLO SLR 50 COB SLG SLG SLO SLR SLG SLO SLR 4 2 SLG SLR 2 CO 3
11. 7 8 453 455 3 Asai T et al Berthold A and Ohmiya 2007 Proceeding of the 14 International Symposium on BIOLUMINESCENCE AND CHEMILUMINESCENCE World Scientific 177 180 4 2007 IYERHBDOA DON AL ITUF IM TITAN 235 243 5 Ohmiya Y Sumiya M Viviani V R and Ohba N 2000 Sci Rept Yokosuka City Mus 47 31 38 6 Viviani VR Uchida A Suenaga N Ryufuku M and Ohmiya Y 2001 Biochem Biophys Res Commun 280 1286 1291 7 Viviani VR Uchida A Viviani W and Ohmiya Y 2002 Photochem Photobiol 76 538 544 8 Viviani VR EJH Bechaera and Ohmiya Y 1999 Biochemistry 38 8271 8279 9 Nakajima Y Kimura T Suzuki C and Ohmiya Y 2004 Biosci Biotechnol Biochem 68 948 951 10 Nakajima Y Ikeda M Kimura T Honma S Ohmiya Y and Honma K 2004 FEBS Letters 565 122 126 11 9 Code No SLG pSLG test 20ug MRV 101 SLO pSLO test 20ug MRV 102 SLR pSLR test 20ug MRV 103 SLG SV40 pSLG SV40 control 2019 EA SLO SV40 pSLO SV40 control enue MB SLR SV40
12. Filter Filter1 Filter no 1 10 1 SLO SLR SLG N 3 SLO SLG SLR SLG 1 1 Microsoft Excel 5 PMA AP1 NFkB m TNFa AP 1 20 TIE NFkB TNFa EDS m 0 1 10 100 PMA nM AP1 SLO lll NFkB SLR Relative activity e
13. D 2 3 3 1 2 Tripluc Luciferase Assay Reagent MultiReporter Assay System Triplucei 1 1 2 AR 1 10 2 MAREA
14. 7 Endotoxin HEHE 5 10
15. pSLR SV40 control eho MRANA SLG HSVtk pSLG HSVtk control ars NRN SLG SLO SLR pSLG test pSLO test pSLR test 20g MESH SLG SLO SLR SV40 pSLG SV40 control pSLO SV40 control pSLR SV40 control 200 MENE SLG SLO SLR pSLG test pSLO test pSLR test pSLG SVAO control pSLO SV40 control pSLR SV40 control 200g MS pSLG HSVtk control D luciferin 20 mg MRL 101 D luciferin 20 mg x5 MRL 101X5 Eth 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail
16. 24 1 0 09ug pAP1 SLO 0 02ug pNFkB SLR 0 09ug pSLG HSVtk control Code No MRV 301 1 0 5pl ZEonmoou gt MECO OI GXOXOXRXR AMAIA OO 5 5 6 67 6 SED N Lipofectamine 2000 No 1 10 G O R pSLG SV40 control Code No MRV 201 pSLO SV40 control Code No MRV 202 pSLR SV40 control Code No MRV 203 0 249 2 37 C 5 CO FC 24 1 SLG SLO SLR 50 B 2 pSLG SV40 control pSLO SV40 control pSLR SV40 control 3
17. tech_osaka toyobo jp URL http www toyobo co jp bio
18. 1 fzo MultiReporter Assay System Tripluc Green 550 nm Orange 580 nm Red 630 nm 3 1 4
19. T2r SLR Filter 2 3 SLG SLO SLR G O R F0O 560 nm LP Filter 1 F1 600 nm LP Filter 2 F2 FO G O R F1 T1gxG T1oxO T1rxR F2 T2gxG T2oxO T2rxR F0 F1 F2 G O R T1o x T2r T1r x T20 x FO T2o T2r x F1 T1r T10 X F2 mue T1g x T20 Tirx T2g Tlox T2r Tig x T2r T1r x T20 T10 x T2g O T1r x T2g T1g X T2r x FO T2r T2g X F1 T1g T1r x F2 T1g x T20 Tirx T2g T10 x T2r T1g x T2r T1r x T20 T10 x T2g R T1g X T20 Tlo x T2g x FO T2g T20 X F1 T10 Tig X F2 Tig x T20 Tir X T2g Tlox T2r T1g x T2r Tir x T20 T10 x T2g G O R xx 2 560 nm LP Filter 1 600 nm LP Filter 2
20. 12 12 TOYOBO Ideas amp Chemistry PCK MultiReporter Assay System Tripluc Tripluc Luciferase Assay Reagent 100 Code No MRA 301 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3977K 1 SEE PPP Erica deere MultiReporter Assay System Tripluc 1 2 oan nr BBW WD 4L rcs Ux 3 OC
21. LO Filter 2 Filter 3 T30 SLO Filter 3 SLR Filter 1 Tir SLR Filter 1 Filter 538 T2r SLR Filter 2 Filter 3 T3r SLR Filter 3 3 SLG SLO SLR G O R Filter 1 F1 Filter 2 F2 Filter 3 F3 F1 T1g T1o Tir G F2 T2g T2o T2r O F3 T3g T3o T3r R F0 F1 F2 G O R G Tig T1o Tir Y F1 O T2g T20 T2r F2 R T3g T30 T3r F3 Microsoft Excel Microsoft Excel 3 x3 MININVERSE 3 x3 Ctrl Shift OK
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