マルチプレートリーダーARVO簡易操作マニュアル
Contents
1. Measurement mode By plate By wells La Dispense Dispense properties Injector 1 Volume 50uL Speed 5 OK Injection mode aspVn disnvol aspVo syringeVo Perfarm an first nlate reneat nnly Protocol editor Operations Dispense Shake Dual Luc iferacse Ascay Protocol editor Fe Hep ls Soroks Messuremert Outputs ad Evers 2sremer t mode By plste fe y wels Repeated cperaiicn Drspenser head control When aessurre keep jector head sbove well Shake properties Durat2. Next gt 31 ARVO Finish Frluml lmr TO ml Wiad 92 PerkinEImer 2030 Manager Latest assay result we mm 14
3. Data Flag 3 C D E i G HH 17 Flag OK x Recalculate Recalculate Flag Analysis Flag 18 Flag OK
4. Linear Regression Polynomial Cubic Spline S Four Parameter Five Parameter OK scale Y Axis to BB usin calculated a and d Calculate EC n Axes X Y Factors Unknowyn 1 Edit
5. 30 3 1 33 3 4 ARVO PC 35 1 UV UV UV Fura2 Ca2 Em510nm Umbelliferone Hoechst 33258 DAPI Em460nm B B B A 2 B2 4 Fluorescein FITC GFP Em535nm B 0 repiun toside ep gne2om B 7 TAMRA Resorufin Alamarblue Em590nm B Alexa594 Em632nm
6. 0 7 7 Close Dispenser Maintenance 30 PerkinEImer 2030 Manager PerkinEImer 2030 Start Wizard Next gt Frlumil lmmr TOTO bltart Wr dd Pr Of Font ee ea i et mah yinl 1 we 1 of 1 G mwlnl W 1 WW
7. Off ARVO On 35
8. Concentrations malysis Settimngs LIE mt ar ct Far oun resnseraei das oO ra le ml CE FIR EV Ter Tiina Here jnmma fewrar Ermarsam sm Fm Em wae Fwwm Faateas 1 ma a mdm 1 PT 1 m s Cohn Dd Method Edit amalysis Settings LU mt areal ct Far nak renseras das oO ra le mal CE FA EN Ter Tlies here pe Pn Po COP Wi Fnre bert tes Fwwm Facteas Tmm carrart FT ur cr kb iaq ia ha Sshry them 1 or HH hen To rten 2 TN Ei re rr an het br FTemrrm ET Prmmai YO CE EE rm iT a Fa eae RE ms tt sr 10 Standard Curve Fit Properties
9. On ARVO On PC PC PerkinEImer 2030 Workstation PerkinEImer 2030 Workstation PerkinEImer 2030 Manager amp le Bia Lws display Kinstic Chart Tewperatye Frotocol ALsorbamce 450 1s gl P 5 10 11 12 Scok Logilhmi Ts P30 PerkinEImer 2030 Manager Explore protocols and results PerkinEImer 2030 Explorer nps sg Fie ck atnE Ac 7 et EE ll
10. 1 2 Flush MilliQ MilliQ Apply MilliQ 2 MilliQ PrimingVolume 0 7mL 7 Flush 7 2 7 MilliQ MilliQ Apply MilliQ Fill Apply 1
11. Analysis rahe a Twit ox pa Ltem MT le tw MT er mwiiwnrc Mfree Wer fr JE LU pn PP ge 1 nat _LIr PA So 4 HLSeiS onces a deeds Ln lt 183 Next gt malysis Settings Ci CH mrLrm mt aur of Far am im resnserasi ds o rom lie mal CE FA EV Tr Totten here ad Fargr Eurwrrmwi Ear mrrmramrr Piond ssi Fmre EmrF mumr Fmmm Fortras Th carrart Fe er or entirs F Ermrmrrmnrbs pmi uma rcTemrm im er ET Fm rm ru CE be ls bln Lede la Ci eo WE Pa Fa ark Completing Press inshto ksep the Ww eerd s setirgs Analysis Wizard Save 14 WorkOut Run or open an existing protocols Organiser N a a Reports Mssay Protocol
12. ARVO Start Measurements 16 4 Data Audit Trail Analysis Report Data Audit Trail Analysis ELISA Report Duplicate Triplicate Standard
13. On ARVO On PC PC Workout WorkOut WorkOut 2 5 je Shortcuts k Heb Orgenmser TN Organiser a Create a new protocol FN Run or open an existing protocol Opeh exksting results tia Create a new microplate layout 3 Exit Workout P15 WorkOout WorkOut A B C A Create a new protocol Welcome to the Quick Start Wizard
14. PerkinEImer 2030 Result Viewer PerkinEImer 2030 Manager Dispenser Maintenance Dispenser Maintenance jmstrument status dle Select and operation Fill Empty Flush ss dl Se dl rm wy ee Empty LT 1 0 Wf I Apply 34 Flush
15. Label Labels Photometry Fluorometry Absorbance 450 0 1s OK THRF Fluorometry Luminometry FP LANCE Absorbance 260 1 0s Absorbance 280 1 0s a a FAbsorbance 450 0 1s ee Absorbance 595 1s Absorbance 530 1 0s Absorbance 530 0 1s Absorbance 57 1 0s Absorbance 57 0 1s Operations Absorbance 450 0 1s 450nm 0 1 Absorbance 630 0 1s 450nm 0 1 630nm 0 1 amp nn nm 5 4 s 4 c lt a s lt c 1 3 a i a J
16. 2 2 Fura2 Ca2 Em510nm MCA Em460nm A A 2 2 2 2 A 1 3 2 1 3 DihydroEthidium Em610nm EtBr Em620nm 6 8 4 O Umbelliferone Hoechst 33258 DAPI Ex355nm MCA Ex380nm Fura2 Ca2 Ex340nm 380nm Fluorescein FITC GFP Ex485nm TAMRA Resorufin Alamarblue Ex544nm DihydroEthidium Ex520nm Eu EtBr Ex520nm Propidium Iodide PD Ex530nm Alexa594 Ex555nm Sm OO OO O10O OO OO O OO ARVO WorkOut 2 5 ER ARV0O PC ARVO
17. MiliQ MilliQ Apply MilliQ 3 MiliQ 7ngVoue 0 77 7 Flush 7 2 1mL em MilliQ 709 709 Apply 7096 2 70 PrimingVolume 0 7 7 gt us7 7 2 77 709 6 Apply
18. Save and Close sssacesss EL S EO 2 6 Microplate Layout Next gt Microplate Layout Se mc1 te Noocats neil 0 LR UMm sr eogirg Wctccius hr Administrative Options Next gt Administrative Options nth ce tsch acEPoma oparaiers to ur m the promcecsl Assay Protocol File Details 3 nf faaar Pslooo the Dcteces Soecty a limmsme or ina nw Pierarm FCM LMT FE Sm Pr Dr FWA A TI Yb Has Completing Yo sue aucoesst dy comeketed tre k er C Analysis Wizard ssysswssa Welcome to the Analysis Wizard
19. EC Flag Recalculate Recalculate Flag if Nm 4 a Y X Y Analysis Wizard ed a ans wa a We aa 1 hal aime a RR na ma i a fa 1 hmm aim Fate A a ee ei aa min Late ms poten a fai a hed had a Et 19 a La Li mt rie of Far ur rer a rts a Errs Fae es EX ET Fa Pi oa EE 4 Dore i thee ww i i FEE kui ri Wi 9 HH Next gt Welcome to the Analy
20. Amalysis Settings HH mr mt praie ot rar im m rmi Flier Eurrrrmwi Ea preariraiarrs Dilution Factor Properties Factors 1 Dilutinn Factnors Properties Input Em CD TT Matrix nknowny nknowng Unknownd nknown1 Unknown11 nknown12 nknown13 nknown14 Advanced Auto Series Output Matrix OK AMI 1 Dilutinn Factnors Properties Input Matrix Factar Output Matrix SperciFy the details of the resulting output matrix Name Dilutions Lnits of measurement Result Reporting cc D Analysis Setting Advanced Analysis Settinqs Dortigure crh
21. Delete Edit F Ei be EE L EI 4 TE pm ct Flercyimem t Fr 2 Next group Replicates Fil Settings 2 Next gt 2 A
22. 4 3 lt S 3 EsIX File Save Protocol editor Measurement Settings Next gt Measurement Settings Srp the Uns Acgis torc corka mW corracied m B Microplate Layout Next gt Create Micioplatm Layout deusi Tu NEOP atm Scil 0 mh Ue sr wosieg Ii Microplate Layout Editor Add Layout
23. Next gt Welcome to the Analysis Wizard The Wsa nl gue ycu threugh tne process oF settng Up VOLT sozsy retrst Analysis Selection ELISA Quantitative Next gt Einh ri rm La EL St cl mmm IF LT Ri rm a LT mr Rakio be rt heater te Te Fre Diflarerces re be Her CO AT TE rT Hila Correrl a OAT bwdrcurrr FEAT ET HE Tor OL pam be LE a LT Analysis Overview Next gt ma is Ce PE Here ure am trarakaer ect kmrm sv ha Uhh a aakrTr ie to camiurmima He frargkm or hr m ite rele al Whtris PMPresinml on Minar hm MT Oe or A WF Gn Is Er te 3 Ee m terre of hn rpoint roat rr wad vy bo ep Er labres ep aic TNImIIIIIIHS Eames a Gp rear for sah mam ma Gt Cur HI mmm CreTeried 1 FE OurwE mi brsrd ars pied ansmrst aE eo id OrnT ts rd rs rs m Casie gt amrMas a re alte rn ri ilutinn FarT Ts Oo Cs ti ie ried drhon Ts m carn amphi Fon Fp Analysis Setting
24. By wls By sr Operation Proper iector 2 Speed 5 Volume 50 4L Incremert 0 4L Replrcate 1 necton mods jMnl peated operair Te Wes Xe se Dispenser head controt When megsurrW keep ector heed sbove well Operations CPS 5 0sec Copy 2 Label 1 Label ID Samples leasurement Outputs and Events Maasur ement mode By wells By strips Operation properties Dispenssr head control hen measuring keep injector head sbove veell 28 Operations Paste 2 CPS 5 0sec Operations Operations ID Samples Measurement Outputs and Events Measurement mode By pte By wells C By strips Operatiors
25. PerkinEImer 2030 Result Viewer Side uit HE IE i EE r FEEEEE EE _ _ HE EE EE EE EEE ES NT a E EE EEETE NTLM EE WN WF WE TH TE EE PerkinEImer 2030 Result Viewer Export File Export Excel Save
26. PerkinEImer ARVO X WorkOut 2 5 ELISA ARVO PC 3 Workout 3 14 U ER EID AEieouoBusspiutroi ausisewm meiRissiSkmeka 3 BZ ER io img 6 C 9 D 12 KMOMUI m II 15 MFI OOI 4 io 2 io 17 AMO PO 2 2 ARVO PC 23 23
27. Fc cc 2 i PL k Fc lia 35 V1 ARVO PC ARVO PC ARVO Off PC x PC ARVO Off ARVO On YY ARVO ARVO PC ARVO
28. Next gt Welcome to the Quick Start Wizard The Vioard hape you Creabe an Accay Prototo ams nnn shens 3 Measuremeht Settings Microplate Layout Administrative Opbtions 9 Open existing results Assay Protocol File Details ea Create a hew microplate layout 3 Exit Measurement Settings WorkOut Create Measurement Settings Sp the Ds Ac JH PO Corto Comractmd mr 3 mL Label Operations 450nm 0 1 630nm 0 1 Measurement mode By Plate
29. Source P10 14 Analysis Settin s crisn or PaPeim Fran wa J re TUF 1 das ml Fa CE FI En CE ii rere Ei JrP FE a a t Analysis Setting Next gt malysis Settimngs CLE mt ara cf ror an rensereel das oO ral mm CE FA EN Cr Ti Hee TT El rw Fa awk Fug Fmms Completing FT Ft tr hesp He Wear oer Tired mph CFlaie 1 Raards Gaph he Ly Report Readings rapoies Acerrbente amp 405 Js 12ei ll Tyeas eon Hat B csrnors TH I ODO 4 TE 4
30. Dispense 3 shake Delay Bi CPS SOsec Dispenss Shake 4 Dslay Operation properties Dispenser head contrd Dtspenser head control hen measuring keep injector head above weell When nessurre keep mector head sbove well File Save Protocol editor Frint Frint Preysew 1 Print Setup_ ement Outputs and Evants By wells C By strips Operatcwis F Dispense 3 shake Delay 8 CPS 50sec Dispense Shake Dslay Operation properties crs 50sec Save the active docurment 29 PerkinEImer 2030 Manager Dispenser Maintenance Dispenser Maintenance jmstrument status dle _Select and a CFill Empty CFlush dl Se PP A gy os_
31. Names ect Qjsrbsete Enst R nstrumcnEi mi Aoorhare STi 1s lt Well Types ldrd Ost rm Opeh existing results tt Create a rew microplate layout 3 Exit RY a a Blork Correct on ol wefs comecced by a cormemcn ork crorp andsrd Crs ve Pt Lres Regresar wtn Staras Ei an Fsrbars Db fators coko ddsd for min CU Cako dt es WCU ot Corcs Standard Blank Unknown ARVO Start Measurements CTYDmcgmentg gnd SptlinrccYNmotn proicY Tecl Quoentitate gr Es sos Ho ee a ww bi Sanpls Ds Ansbais Wesd Ed Pre Cs Ws WUworliance _ ee LL Run Scrubber Wizard Scrubber Wizard Next gt 4 WT Welcome to the Scrubbsr Wizard The
32. ke ke te ll Ue ke ks ke DD Use A 5s i ww 8 Explorer New protocol 4 PErkinEImer 2030 Explorer File Edit Tools Wiew Help X Protocols Assay Begindate End da PerkinElmer Assay examples Users a Properties for new protocol Dual Luciferase Assay OK Explorer lt PerkinEImer 2030 Explorer File Edit Tools View Help X Protocols assay Begin date PerkinEImer Assay e
33. 70 Close Dispenser Maintenance ARVO PC ARVO PC ARVO Off PC x PC ARVO
34. By srips Operaton procertes Dwlay duraton Repeasted oper ston Drspenser head controt When messurre keep nector head sbove well Labels Luminometry CPS 5 0sec 5 OK Labels TFF Fluoromet Photometry a CPS 5 0sec e CPS 2 0sec CPS 0 2sec dd Lapy Hemaye Propertes HH BR D Cancgl Help 7 Protocol editor Operations Dispense Shake Delay CPS 5 0sec 1 Fie beb S D Samoks Measurememt Cutputs and Everits D4 Dispenser head controt When messurire keep njector head sbove well 2 Dispense Injector 2 Shake 1 Delay 1 Fie Hep iS D Senohks Measuremert Outpuls ad Everits
35. Farsmctfer of raur aralyza Imp ne Exoression Expeession Ccncerkraborg rerhod Weiatr Errcr Ears ep l Up mirr 6 tronsformnad nn sal ce bebe am ks For au 1 abeco nce ET 0 32 YIORa 2 Abecrbence 2 50 0 35 19 Source Measurements from existing assay results Select File and Import Analysis Settings DardigLrc carh Farsmcfer of you aralyss H mesure cats vailsbie you cr PfEymm your set Imp fdces Excression Ourpur Naee Ineut sbte Expressor rt t 4stite rp warn crcerkratior Metad weich rcrBsrs Aes Rarce Cup lt Cota sn s spscfr he standards dsks set rep WorkOut Result File C My Document My Assays Results Result File ars Open K 0 D Erd_Fort_Demo hy Computer My eo O say Fesun Fiks gt Result File
36. Wed 8 53de rou Hrouge sepa lc ed tHe rece ct Per walk am tx PLMm oie Ou oy ivwese roe p24n by Gcheny nn oh nhus ets Or ry amp gr lt tha rou orer blodg gt amn e reas J Mori my lt or CI SOcr eit OW Tune rie Fe hm mt Fo ai shy etre so rac 5 Scrubber Wizard Unknown Unknown Reset Unknown Next gt mj mm we enw tioche to mw law trer the amp nad hm hrcat 0 EE EC En ALMr nw 3 rlglsliglit js Finish
37. ion 2sec Speed Normal Diameter 3mm Type Linear OK Shake properties Parameters Fast Linear Orbital Douhls gs Perfarm an first plate repeat only Cancel Protocol editor Operations Dispense Shake Delay Fie Heb SO D Samoks Measuremer Qutputs ard Everits Measuemer mods By plste 5 9 we Repeated opwrain SR Drspenser head controt hen ncessurre keep jector head sbove well 206 Delay properties Duration 3sec OK Delay properties Parameters Duratior D1 3600 sec Perform on first plate repeat only Cancel Protocol editor Operations Dispense Shake Delay Label Fie Heb S D Sonoks Messuremert Outputs amd Everits Meqsuremerl mode By plste F By wwls C
38. sis Wizard The WWza loude ycu threugh te process OF settng Up VOLT 22257 rs sg ma is Ce PE Here re the arakmrmr ect tm sve hn gh a taakr r Ie to crm He aa or ior m it real iank Correrkian om mmrhamcp 5 ID jEJ IA Scrrmmrrrs 3 harhmrrard rmt Toni ETCPnTT mmm LSEm pm rrore bbe gm he nc haraluml Cure Hit cm Ulik Lerrusr lal om nines Et Er MR RE RR Fe Hs CUTET ts rd Prmr Has i ra ie marc Dilution Famdlnrs rm Sl Fp AU LU Ta CA lmrs Ee Fd Cre rr tO uu ET Fd EL 1 nat on Cancs Ca re ea bor FE I houhi smoBt mm Analysis Setting Concentrations Method X Y Axes Unknown Factor Advanced
39. xamples Users 4 Dual Luciferase Assay EE ser User 3 User 4 User 5 User 6 Protocol editor Luciferase Assay 96 Measurement V4 Dual Luciferase Assay 1 50uL 3mm 2 3 5 2 50uL 3mm 2 3 5 1 50uL
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