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G-SERIES 取扱説明書

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1.
2. pH Vitrolife G Series 6 0 IVF ET 21 22 1 75 000 200 000 mL 37 C 6 CO
3. 40 40 30 500 80 10 Edgar DH Karani J Gook DA Increasing dehydration of human cleavage stage embryos prior to slow cooling significantly increases cryosurvival Reprod Biomed Online 2009 Oct 19 4 521 5 Gardner DK Maybach J Lane M 200
4. LAF Vitrolife G Series 6 0 IU IVF ICSI
5. CO pH 7 2 7 4 pH G Series G MOPS G MOPS PLUS G PGD W G Freezekit Blast G Thawkit Blast 6 CO 0 m 1 000 m CO 0 6 pH pH 10 G MM HSA solution
6. SpermGrad 90 45 DNA 9096 SpermGradW 90 90 45 909 459 SpermGrad RTU SpermGrad 100 GQ IVF G IVF PLUS G IVFW G MM HSA solution G IVFW G IVFW PLUS 37 6 CO
7. 46 31 721 80 20 866 VITROUS 866 848 7687 46 31 721 80 99 866 VITROFAX 866 848 7632 E mail support fertility vitrolife com E mail support us fertility vitrolife com Vitrolife Sweden AB Vitrolife Inc Box 9080 3601 South Inca Street SE 400 92 Goteborg Englewood Sweden Colorado 80110 USA http www vitrolife com Vitrolife G Series 6 0 65 66 Vitrolife G Series 6 0 Vitrolife G Series 6 0 67 TOGETHER ALL THE WAY Vitrolife Inc 3601 South Inca Street Englewood Colorado 80110 USA 866 848 7687 866 VITRO US 866 848 7632 866 VITROFAX Vitrolife Sweden AB Box 9080 SE 400 92 Goteborg Sweden
8. Vitrolife ISO 13485 2003 and 21 CFR Part 820 QSR Vitrolife G Series 6 0 pH pH USP Ph Eur pH 6 CO CO
9. 10 6 12 50 196 G ThawKit Blast 50mg mL G MM 100mg mL HSA solution Blastocyst thaw solution 1 BTS 1 9 0mL BTS 1 1 0mL G MM 1 0mL HSA solution Blastocyst thaw solution 2 BTS 2 9 0mL BTS 2 1 0mL G MM 1 0mL HSA solution Blastocyst thaw solution 3 BTS 3 9 0mL BTS 3 1 0mL G MM 1 0mL HSA solution Blastocyst incubation medium BIM 9 0mL BIM 1 0mL G MM 1 0mL HSA solution Vitrolife G Series 6 0 BTS1 200mM 109 BTS2 100mM
10. pH HYASEW HYASEW HYASEW 10 G MOPSW G MOPS PLUS G GAMETE 10 HYASE 30 1 HYASE G MOPS G MOPS PLUS G GAMETEW 2 HYASEW G MOPSW G GAMETEW 3 HYASE
11. pH 12 2 3 pH 10 Vitrolife G Series 6 0 pH 1 pH 7 00 pH 7 00 1 2 pH 7 00 pH 7 00 pH 10 0 2
12. G IVFW G Series G MMW HSA solution 5 9 5mL 0 5mL G MMW 2 5mg mL HSA 5mg mL G IVFW G MM HSA solution 10 9 0mL 1 0mL G MM 5mg mL HSA 10mg mL G RINSEW G MOPS G 1 G 2 G PGD mL G MM HSA solution mL mL 9 5 0 5 10 0 19 0 1 0
13. 100nL ny IVF 15 20 day 1 PN ICSI 12 18 pH G MOPS GMOPS PLUS G MOPS G MOPS PLUS pH MOPS CO CO pH 7 2 7 4 G GAMETEW G MOP
14. 30 5 62 Vitrolife Swemed 1 G MOPSW G MOPSW PLUS G GAMETEW 6 M2 M1 GV ICSI
15. ICSI ICSI 6 ICSI G 1 G 1 PLUS ICSI G 1 7 2 PN 2 3 1 G 1 G 1 PLUS G 1 G 1 PLUS Vitrolife G Series 6 0 41 ICSI ICSI 3
16. G MOPS G MOPS PLUS 1mL 60 2 Vitrolife G Series 6 0 9 pH pH pH pH
17. MEA ART CAP College of American Pathologists 1 MEA MEA MEA ART s MEA
18. Vitrolife PETG G IVFW PLUS G Series PLUS 5mg mL HSA G IVF PLUS 10mg Mg mL HSA GS5 G MM HSA solution 7
19. G5 Series IVF IVF Vitrolife Vitrolife Vitrolife
20. G IVFY G IVF K081116 PLUS G 1m K081114 day 2 3 G 1 PLUS G 2m K081117 day 3 G 2 PLUS EmbryoGlue K031015 G MOPS K081115 G MOPS PLUS G GAMETE K021893 G RINSE K022295 Vitrolife G Series 6 0 G Series Vitrolife 59 60 G FreezeKit Blast G ThawKit Blast G PGD SpermGrad HYASE ICSI FREEZEKIT 1 THAWKIT 1 FreezeKit Cleave ThawKit Cleave OVOIL 510 k K032154 K032155 K033101 K023403 K000627 K043116 K000623 K000618 K991351 Vitrolife IVFW ASPW TM SpermRinse CCM K991348 K991345
21. 95 10296 pH7 00 4 mV pH8 00 pH pH 0 05pH CO 16 a b a b Vitro
22. 1 LAF 2 LAF 3 Vitrolife G Series 6 0 5 4 6
23. 37 G MOPS G MOPSW G MOPS PLUS G MOPS G MOPS PLUS G IVFW G IVFW PLUS G IVFW G IVFW PLUS 1 0mL 2 G IVFW G IVF PLUS ny pH
24. MEA 2 1 3 MEA MEA ART Cn 1 College of American Pathologists 1998 Reproductive Laboratory Section 90 Proposed Checklist p26 8 40 2 Gardner DK and Lane M 1999 Embryo culture systems Handbook of In 30 Vitro Fertilisation Second Edition Eds Trounson AO and Gardner DK CRC Press Boca Raton p 205 264 20 1 3 Davidson A Vermesh M Lobo R A and Paulson RJ 1998 Mouse embryo c
25. G MOPSW 1mL 60 2 G MOPS G MOPS G MOPS G MOPSW PLUS G MOPSW G MM HSA solution CO 2
26. 62 Vitrolife Swemed Vitrolife G Series 6 0 37 38 ICSI ICSI HYASE 62 Vitrolife Swemed 130 175um
27. 9 IVF ET Vitrolife G Series 6 0 1 EmbryoGlue G 2 G 2 PLUS 2 EmbryoGlue 1mL 3 EmbryoGlue 2mL 4 37 69 CO 4 18 5 EmbryoGlue 6 CO 10 EmbryoGlue 4 EmbryoGlue 6 1mL
28. USP Ph Eur Vitrolife LAL 0 005EU IU mL USP Ph Eur FDA Guideline on validation of the limulus amebocyte lysate test as an end product endotoxin test for human and animal parenteral drugs biological products and medical devices USP Ph Eur
29. Vitrolife G Series 6 0 51 52 4 TS1 TS1 5 TS1 5 6 TS2 5 7 TS3 5 10 8 Cryo PBS 6 37 4 Cryo PB 5 CO CO
30. day 6 EmbryoGlue G 2 IVF ET G IVF ICSI G 1 Day 1 G 1 G MOPSW G MOPSW G GAMETE G 2 day 6 3BB EmbryoGlue G 2 IVF ICSI G MOPS G GAMETE G 1
31. 64 Vitrolife G Series 6 0 Vitrolife Vitrolife Vitrolife www vitrolife com Vitrolife Vitrolife Vitrolife www vitrolife com IVF CE 2 CCM Stem Cell Cutting Tool www vitrolife com
32. ICSI pH G MOPS G MOPS PLUS Vitrolife G Series 6 0 pH G MOPSW G MOPS PLUS MOPS 37 pH CO G MOPSW G MOPS PLUS CO 37 G MOPS G MOPS PLUS 37 G MOPS G MOPS PLUS
33. OVOIL 2 40mm ID 50uL G 1 G 1 PLUS OVOIL 2 40mm 25uL G 1 G 1W PLUS 6 OVOIL 25uL 2
34. 5 300 600g G MOPS PLUS 2 5 10pL OVOIL G MOPS PLUS G IVFW G IVFW PLUS 37 C 6 CO PGD day 3 62 Vitrolife Swemed
35. 18 0 25 Step 1 6 2 0 Step 2 6 0 2 6 0 1 0 Step 3 30 0 3 Step 4 150 50 196 ThawkKit Cleave 3 MOPS Thawing solution 1 TS1 Thawing solution 2 TS2 1 2 propanediol Equilibration solution ES 1
36. 2 K991700 Microcell Vitrolife G Series 6 0 Vitrolife 63 Vitrolife FDA HIV ypes 1 2 HBV HCV
37. G MOPS G MOPS PLUS 37 G MOPS G MOPSW G MOPSY 37 G MOPS CO G MOPSW 37 4
38. 48 Vitrolife G Series 6 0 1 0 5 1 0ml TS1 TS2 ES 2 30 3 30 45 4 TS1 5 5 TS2 5 6 ES 5 7 Freezekit 1 Thawkit 1 8
39. ICSI 3 G MOPS G MOPS PLUS G GAMETE 1 4 4 1 5 1 7 4 ICSIW
40. 1 ES FS 0 5 1 0 ml 2 ES ES 10 4 FS 10 FS FS 10 a b 9 d 9 h i a FS 2 3cm b 9 1 4cm c h FS 1cm d FS 2cm i e 1 4cm Vitrolife G Series 6 0 47 5
41. 0 5 mL 7 1mL 0 5 1 0 mL 8 EmbryoGlue 0 1imL 20uL 9 5 10uL EmbryoGlue 1cm 25 30uL
42. ET Vitrolife G Series 6 0 IVF ET 33 34 6 ET 7 ET 8 30uL
43. 7 BFS 2 7 7 BFS 2 6 ga mw M Vitrolife G Seriesm 6 0 53 54 8 1mL 1cm 15 BFS 2 al b o d e f 9 h i ie a BFS2 2 3cm b 9 1 4cm c h BFS2 1cm d BFS2 2cm i e 1 4cm 9 6 10 2 11 6 LN
44. 2 3 SAL Vitrolife G Series 6 0 57 58 MEA 1
45. EmbryoGlue G 2 day 6 G 2 Vitrolife G Series 6 0 ET ny 1 1 2 60uL 1cc EmbryoGlue G 2 G 2 PLUS
46. 37 6 CO 6 7 EmbryoGlue G 2 G 2 PLUS 37 696 CO 6 8 day 5 1 2 day 5 G 2 G 2 PLUS 24 day 6 9 G 2 G 2 PLUS 37 69 CO 10 30 day 2 40 48 day 3 66 74 day 5 6 120 144 28 IVF ET
47. BFS1 100mM 5 BFS2 200mM 10 Vitrolife G Series 6 0 6 Step 1 2 Step 2 10 Step 3 32 0 5 II gt 3BB 1 BIM BFS 1 BFS 2 BIM BFS 1 BFS 2 800uL 20 5 BIM BFS 1 10 BFS 2 7
48. 1 SpermGrad RTU 2 SpermGrad SpermGrad G IVFW G IVF PLUS 9096 45 90 SpermGrad 9 0mL G IVFW 1 0mL 45 SpermGrad 4 5mL G IVF 5 5mL 2 ID 2 4
49. ICSI 3 SpermGrad GIVF G IVFW PLUS ICSIW G IVFW G IVFW PLUS 10 300 600g G IVFW G IVFW PLUS 200 500uLni 42 Vitrolife G Series 6 0 G MOPS PLUS
50. Vitrolife G Series 6 0 IVF ET 35 36 IVF ET Vitrolife G Series 6 0 ICSI 37
51. G MOPS G MOPS PLUS G GAMETE 1mL 4 6 50 100uL A B G MOPS G MOPS PLUS 37 1 5 G GAMETE pH 69 CO ICSI a ee 3 Or 9 d A a HYASE b 1 G MOPSmG MOPSm PLUS G GAMETEW Vitrolife G Series 6 0 B a HYASE b e G MOPS G MOPS PLUS G GAMETE 4 62 Vitrolife Swemed 3 5 HYASE
52. 1 2 propanediol PrOH Testart et al 1986 FREEZE KIT1 Cryo PBS 25mg mL HSA Freeze solution 1 FS1 1 5M PrOH Cryo PBS Freeze solution 2 FS2 1 5M PrPOH 0 1M Cryo PBS Vitrolife G Seriesm 6 0 49 50 2 18 0 25 Step 1 7 0 10 2 Step 2 7 0 10 2 Step 3 30 0 0 3 Step 4 30 0 80 0 30 0
53. 90 1 5mL 45 1 5mL 1 0mL A 2 4 2mL 9 _b 0 A 300 600g 20 a b 45 c 90 16 Vitrolife G Series 6 0 3 300 600g 10 20 4 2 B G IVF G IVF PLUS 5mL 909 5 300 600g 10 6
54. ID 62 Vitrolife Swemed V Tip Vitrolife G Series 6 0 IVF ET 19 20 G MOPS G MOPS G 1 MOPS pH
55. 4 G RINSE 5 2 5 10 0 mL G MOPS 6 37 IVF ET Vitrolife G Series 6 0 37
56. 2 IVF ET Vitrolife G Series 6 0 MOPS pH 3 G 1 G 1 PLUS 1 0 2 2 6 4 2 5 1 3 3 2 4 day 3 EmbryoGlue G 2 G 2 PLUS day 3
57. 4 5 6 Vitrolife G Series 6 0 IVF ET 31 32 day 2 day 3 PLUS EmbryoGlue G GAMETE G RINSE PLUS G MM HSA solution VF G RINSE G MOPS G IVF ICSI G 1 G GAMETE
58. 3 7 1 Z 1 O Z1 2 Z3 Z4 Z Oo Oo Oo O Oo oo o o 2 o 8 O _O DAO oO 2 A 0 5mL 1 0mL B B 0 05 0 1mL
59. IVF ICSI IVF swim up swim up swim up
60. 2 K991700 ICSI K991700 ICSI 1 ICSI K991104 ICSI 1 K991700 ICSI K991104 KO022643 62 Vitrolife G Series 6 0 510 k PZD K991700 _PZD
61. 3 37 69 CO 6 pH 18 Vitrolife G Series 6 0 IVF ET 25 26 ny pH
62. day 5 day 5 Vitrolife G Series 6 0 IVF IVF 1 2
63. G PGD MOPS G MOPS 8 G PGD CO CO Vitrolife G Series 6 0 43 44 1 2 a PrP G PGD OVOIL G PGD 37 G PGD Ca2 Mg2 MOPS 1 2 G PGD G 2 G 2 PLUS G 2 G 2W PLUS
64. G Series PLUS G MM HSA solution Vitrolife G Series 6 0 G Series PLUS G RINSEW G GAMETE EmbryoGlue PLUS G Series G MM HSA solution HSA HSA G MM HSA 0 15ppm
65. Vitrolife Vitrolife MEA Vitrolife Swemed IVF Vitrolife ICSI Vitrolife G Series 6 0 Vitrolife Vitrolife
66. SR 1 2 O 3 4 5 6 ICM A B lt C A B C IVF ET Vitrolife G Series 6 0 4AA 3
67. 62 Vitrolife Swemed day 1 3 150 200hm 1L G MOPS G MOPS PLUS G GAMETEW 2 3
68. VF Swim up migration IVF ET Vitrolife G Series 6 0 10 12 13 14 16 19 19 20 21 22 22 23 24 25 26 28 29 30 31 32 33 33 35 ICSI ICSI ICSI Freezekit Cleave Thawkit Cleave Freezekit 1 Thawkit 1 G Series Vitrolife G Series Vitrolife 37 38 40 42 43 43 45 45 47 49 52 56
69. 3 4 LN Vitrolife G Series 6 0 45
70. 2 4 G IVF G IVF PLUS 2 0 mL 37 69 6 CO 30 60 swim up 37 696 CO 30 60 swim up a G IVF G IVFW PLUS b 14 Vitrolife G Series 6 0 10 G IVF G IVF PLUS 5mL 300 600g 10 G IVFW G IVFW PLUS G IVF G IVF PLUS 5 0mL 300600 g 10 1
71. G 2W G 2 PLUS Vitrolife G Series 6 0 55 Vitrolife US Pharmacopeia USP European Pharmacopoeia Ph Eur SO 13485 2003 21 CFR Part 820 QSR Vitrolife Vitrolife
72. Vitrolife G Series 6 0 EmbryoGlue G 2 G 2W PLUS O Gardner DK Schoolcraft WB 1999 In vitro culture of human blastocysts in Towards Reproductive Certainty Infertility and Genetics Beyond 1999 eds Jansen R and Mortimer D Parthenon Press Carnforth pp 378 388 pH
73. G 2 G 2 PLUS Day 3 G 2 G 2 PLUS 1 day 3 ID 25uL G 2 G 2 PLUS OVOIL 2 37 69 6 CO 6 1 1 0 2 40mm ID 50uL G 1 G 1 PLUS OVOIL
74. 1 0 G RINSE Vitrolife G Series 6 0 13 Swim up migration Swim up 1 20 23 20 2 2 3 ID 4 1 0mL
75. PVP ICSI AI crush tail 50 75 Vitrolife G Series 6 0 ICSI PVP ICSI 62 Vitrolife Swemed 1 ICSI 1 2uL 2
76. G MOPS G IVF G IVF PLUS G MOPS G MOPS PLUS 37C 6 CO G GAMETE G MOPS G MOPS PLUS 5 1 1 G RINSE 37 6 CO 2 G MOPS PLUS 37 CO 3 G MOPS 37 CO 37
77. 2 a sa a lt 1 SL b 1 2 OO s 1 3 b A 1 B 2 a 1 6 a G MOPS G GAMETE 1 6 G MOPS G GAMETE b ICSI b ICSI 4 G MOPS 37 15 G GAMETE 37 69 CO 4 ICSI ICSI ICSI ICSI PVP PVP
78. 3 Nomarski Hoffman 200 2PN 1 PN 3 4 G 1 G 1W PLUS G 1 G 1 PLUS Scott L Alvero R Leondires M Miller B 2000 Hum Reprod 15 No 11 pp 2394 2403 Hum Reprod 15 No 11 pp 2394 24
79. OVOIL 0 22um 9 9 b b A OVOIL B a OVOIL a OVOIL b b 10 100uL 24 IVF ET Vitrolife G Seriesm 6 0 6 CO 5 O2 69 CO 5 O2 89 N2 6 CO 1 G RINSE 2 ID 25uL G 1 G 1W PLUS
80. day 5 37 6 CO G 2 G 2 PLUS 5 G 1 G 1 PLUS EDTA 2 3 G 2W G 2 PLUS 5 O CO 6 day 4 G 2W G 2W PLUS 3 Transfer Freeze Hold day 6
81. 6 BTS 1 BTS 1 BTS 1 BTS 1 1 1 7 BTS 2 5 8 BTS 3 5 9 BIM 5 10 37 BIM 5 11 1 G 2 G 2 PLUS 1mL 12 EmbryoGlue
82. al b 9 d e 9 h i IN I A A OA a FS2 2 3cm b g 1 4cm c h FS2 1cm d FS2 2cm i e 1 4cm Vitrolife G Series 6 0 5 6 7C LN 7 2 196 THAW KIT 1 Thaw solution 1 TS1 1 0M PrOH 0 2M Cryo PBS Thaw solution 2 TS2
83. G MOPS PLUS G GAMETE 1 G MOPS G MOPS PLUS G GAMETEW 1mL G MOPS W G MOPSY PLUS G GAMETE 2mL Vitrolife G Series 6 0 IVF ET 27 3 G MOPS G MOPS PLUS G GAMETE 1mL G MOPS G MOPS PLUS G GAMETEW 2mL G MOPS CO CO 4 day 3 G 2 G 2 PLUS
84. 2 40mm 25uL G 2 G 2 PLUS 9 OVOIL 25uL 2 5 37 6 CO 6 1 1 0 2 gay 2 37 6 CO 2 1 0 G MOPS
85. C 2 G IVF G IVF PLUS 1mL D mm MO a B C G IVF G IVF W PLUS D G IVFW G IVFW PLUS a 7 75 000 200 000 mL G IVFW G IVFW PLUS 8 0 5 1 0mL 37 6 CO 2 0 5 1 0mL 100uL Vitrolife G Series
86. ICSI ICSI G 1 G 1 PLUS ICSI M1 GV 1 G MOPS G MOPS W PLUS VOILW 37 15 G GAMETE 37 696 CO 4 2 ICSI 20 5 3 ICSI ICSI ICSIW 1 10uL G MOPSW G MOPS W PLUS G GAMETEW 6 10uL Vitrolife G Series 6 0 39 40 OVOIL 2 A B 1
87. day 5 2 Transfe j Freeze 1 3 AA 1 1 6 ICM 2 2 3 3 6 ICM Vitrolife G Series 6 0 IVF ET 29 30
88. Q IVFW G IVF PLUS 0 5 1 0mL G IVF G IVF PLUS 75 000 200 000 mL G IVF G IVF PLUS 0 5 1 0mL 37 6 CO 2 100uL Vitrolife G Series 6 0 15 SpermGrad SpermGrad
89. ICSI ICSI ICSI ICSI ICSI ICSI
90. 46 31 721 80 46 31 721 80 99 E support support us fertility vitrolife com E support support fertility vitrolife com E mail fertility vitrolife com http www vitrolife com Vitrolife
91. 6 0 17 18 Vitrolife G Series 6 0 IVF ET Vitrolife G IVF G IVFW PLUS G 1 G 1 PLUS G 2 G 2 W PLUS day 3 ICSI G 1 G 1 W PLUS EmbryoGlue G2W G 2 PLUS pH Day 0
92. 596 BTS3 100mM BIM MOPS G 2 1 BIM BTS 1 BTS 2 BTS 3 BIM BTS 1 BTS 2 BTS 3 800uL 20 5 2 LN 3 10 4 30 30 5 1mL
93. 0 5M PrOH 0 2M Cryo PBS Thaw solution 3 TS3 0 2M Cryo PBS 1 1 LN TS1 TS2 TS3 Cryo PBS 2 30 LN 3 30 30 1mL
94. 30uL 1 G RINSEW ET
95. 9 G 1 G 1 PLUS day 3 G 2 G 2 W PLUS G 1m G 1 PLUS 10 G FreezeKit Blast 50mg mL G MM 100mg mL HSA solution Blastocyst incubation medium BIM 9 0mL BIM 1 0mL G MM 1 0mL HSA solution Blastocyst freezing solution 1 BFS 1 9 0mL BFS 1 1 0mL G MM 1 0mL HSA solution Blastocyst freezing solution 2 BFS 2 9 0mL BFS 2 1 0mL G MM 1 0mL HSA solution BIM MOPS G 2
96. MOPS NaHCO Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes G Series Yes Yes Yes Yes Yes 61 Vitrolife www vitrolife com National Regulatory Requirements 510 k K991273 K991700
97. 10 1 Cryo PBS FS1 FS2 2 Cryo PBS 3 FS1 10 20 4 FS2 1mL 1cm
98. 59 61 62 64 65 Vitrolife David K Gardner Vitrolife G SeriesW Vitrolife Vitrolife MEA ISO 13485 2003 21 CFR Part 820 QSR
99. K000621 CSI day 3 G Seriesm G RINSE G MOPS G MOPS PLUS G IVF G IVF PLUS G GAMETE G 1 7G 1 PLUS G 2 7 G 2 PLUS EmbryoGlue G FreezeKit Blast G ThawKit Blast FreezeKit Cleave ThawKit Cleave G PGD Vitrolife G Series 6 0
100. pH day 2 day 3 IVF ET 5 2 1 1 Day 1 G 1 G 1 PLUS 1
101. 0 SERIES Dr Gardner G Series Edition 7 January 2013 Vitrolife 2002 2013 Vitrolife AB All rights reserved Vitrolife Vitrolife Sweden AB Vitrolife AB Box 9080 SE 400 92 Goteborg Sweden Tel 46 31 721 80 00 Vitrolife Inc 3601 South Inca Street Englewood 80110 USA Tel 1 866 VITRO 866 848 7687 G5 G Series G5 GIll GII Series GII G MM HSA solutionw G IVFY G IVF PLUS G 1 G 1 PLUS G 2 G 2 PLUS G GAMETE G MOPS G MOPS PLUS G RINSE G FreezeKit Blast G ThawKit Blast G PGD SpermGrad HYASE ICSI FreezeKit Cleave ThawKit Cleave V Tip Vitrolife Sweden AB EmbryoGlue Vitrolife Sweden AB Vitrolife G Series 6 0 4 Vitrolife CO G MM HSA solution G MOPS G MOPS PLUS pH
102. 03 2 2PN Day 3 2 pH IVF 15 20 ICSI 12 18 ICSI IVF G MOPS G MOPS W PLUS G GAMETE Vitrolife G Series 6 0 IVF ET 23
103. 1 Hyaluronan and RHSA increase blastocyst cryosurvival Proc 1 7th World Congress on Fertility and Sterility Melbourne pp 226 Lane et al 2003 Cryo survival and development of bovine blastocysts are enhanced by culture with recombinant albumin and hyaluronan Mol Reprod Dev 64 70 78 Gardner DK et al 2003 Changing the start temperature and cooling rate in a slow freezing protocol increases human blastocyst viability Fertil and Steril 79 2 407 410 46 Vitrolife G Series 6 0 Freezekit Cleave Thawkit Cleave 8 FreezeKit Cleave 2 MOPS Equilibration solution ES Freeze solution 1 FS 1 2 propanediol PrOH
104. 20 0 28 5 1 5 30 0 38 0 2 0 40 0 47 5 2 5 50 0 57 0 3 0 60 0 66 5 3 5 70 0 76 0 4 0 80 0 85 5 4 5 90 0 95 0 5 0 100 0 Vitrolife G Series 6 0 7 G IVFW mL 9 0 18 0 27 0 36 0 45 0 54 0 63 0 72 0 81 0 90 0 G MM HSA solution mL 1 0 2 0 3 0 4 0 5 0 6 0 7 5 8 0 9 0 10 0 mL 10 0 20 0 30 0 40 0 50 0 60 0 70 0 80 0 90 0 100 0 G FreezeKit Blast G ThawKit Blast 50 52 9 0 mL Freeze Thaw solution 1 0 mL G MM 1 0mL HSA solution 900uL G MM 100uL HSA solution 100uL 1 G MOPS G MOPS PLUS G MOPS G MOPS PLUS CO CO
105. S G MOPS PLUS G GAMETE 37 69 CO 1 G MOPS G MOPS PLUS 5 37 62 Vitrolife Swemed IVF ET Vitrolife G Series 6 0
106. life G Series 6 0 11 9 pH pH CO 30 30 CO pH 10 pH pH 11 IVF IVF
107. ulture as quality control for human in vitro fertilisation the one cell versus the two cell model Fertil Steril 49 516 521 T T T T T 4 Lane M and Gardner DK 1997 Differential regulation of mouse embryo 40 45 50 55 60 65 70 development and viability by amino acids Reprod Fertil 109 153 164 Vitrolife G Series 6 0 G Series Vitrolife 510 k G MM K021894 G MM 50 mg mL G MM HSA solution K021896 HSA solution 100 mg mL HSA solution HSAsolution FDA HIV types 1 2 HBV HCV HTLV types II

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