Biacore J 取扱説明書
Contents
1. hm ie2. 60 R 4 2 a O O
3. OFF TEL 03 5331 9336 ED 9 00 17 30 WwWw gellfesclences co Jp e maill Web
4. Bulk Effect Bulk Effect 2 Kp Kp 10 100 u g ml 5
5. Edit Delete Biacore J Instrument Handbook 14 3 4 Run Stop BIA HHE in no maht to stop the sensorgrarmy Tes Yes Standby 6 Status Window Flow rate Standby Standby Standby tatus Flow rate ctandby Tempnerature 25 0 5 File Close
6. 10 ug m 10mM pH 6 0 10mM pH 5 0 10mM pH 4 0 medium 2 pH IgG pH6 0 5 0 4 0 10mM pH5 0 pH4 0 pH5 0 50mM NaOH 1
7. Biacore J Instrument Handbook 15 4 90 CM5 VM N g
8. 50ml 4 5 Biacore J Instrument Handbook 35 7 BIAmaintenance Kit Desorb Sanitize Sensor Chip Maintenance 7 1 Prime Tools Prime Flow Systems
9. 2086 CA O Liamd NH at y NH Ligand Amine H Ligand MASS gang thiol H PDOEA Ligand HH 11 Cyetamineg 0 0 1 Li cE SH y CN SS Hand thiol HH H 2 DTE DTT 1 Ligand ct HYdrazihn AH NHNH NHNHCH Ligand Algdehyde 2 NaEHasCH Biacore J Instrument Handbook 16 4 1 CM NHS EDC NHS NHS
10. Maqintenance Chip 10 MiliQ MiiQ Biacore J Instrument Handbook _37 8 System Check HBS EP BIAtest solution Sensor Chip CM5 Sensor Chip CM5 Dock Command Dock 25C Single Channel Fc1 Fc2 High Flow BIAtest solution 2 Y
11. Hesonanees sgnal kn 40 20 10 a i Ei NHS gt 300 600 900 1200 1500 Time s CONH coN Flg8 coOO CD CONHCH CH OH lt gt Sensor Chip CM5 10mM OP EDC N Ethyl N 3 dimethylaminopropyl carbodiimide hydrochloride NHS N Hydroxysuccinimide 1M Ethanolamine hydrochloride pH8 5 EDCINHS 10 ml MiliO 200 u 20 1M Ethanolamine hydrochloride 4 Biacore J Instrument Handbook 17 10 100 h g ml 10mM 10mM
12. 4 Flush Tools gt Flush 7 2 Desorb IN IMa2 10 1 BIAdesorb solution1 0 5 SDS BIAdesorb solution2 50 mM glycine NaOH pH9 5 Sensor Chip Maintenance MilliQ 2 gt MilliQe Sensor Chip Maintenance Dock Command Dock Dual channel Medium Flow BIAdesorb solution 1 5 200ul BIAdesorb solution 1 BIAdesorb solution 2 5 200ul BIAdesorb solution 2 2 MiliO
13. RU 0RU Baseline Yes Biacore J Instrument Handbook 13 RU Sensorgram SHI 45000 ODO 350b00 LDN DO noon 15000 10000 100 200 300 HH SH EO TO SD 300 TO TI mm i oc 282 18957 Report Foint Table Ewents arnd atus otebook Edit Add report point RU Sensorgram SLILIHII SO DN SD 300DD SOO DOO 15000 1HII 1Hn 200 300 HH SH EOD DO HH HI 1HII Time RelResi Heport Fnint Table Mntehnnk Lm Hr i View Reference Line 5
14. Status window Stqtus window 2 3 Tools gt Normalize Hormalize nrmalze splrate 5 HBEmormallzing snlutinn 5 pl air and nnrmallzinn solution Intn a pipette hn see Help for delallg Flace the tip In the nchlnn port Llick on 5tart an wal for the procedure to Fish Start Larcl 100 200u BIAmaintenance Kit BIAnormalizing solution BIAnmormalizinag solution 75 ual A 5 Ll BlIAnmorrmalzinag LI Biacore J Instrument Handbook CE 7 Je Stat I i Homalize i Homalize 3 Close Normalize Biaco
15. 2010 GE GE UKAs 169 0073 3 25 1 ISO 9001 2000 TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com
16. Precomcentration of rouse dG pH 5 7 pH Biacore J Instrument Handbook 19 4 2 Run Start mulart Run Analysis mode Single Channel Next gt Start Hun Tubing connections Hake sure that the tubings on the connector block are Connecited as shown IF wou change the tubing cornnections CICK Carcel Drirme the Ho swstem start the sensorogram Next gt martart Hun Flow rate Medium Start Biacore J Instrument Handbook 20 Tare Results As ET OE Ella MLComputer Resulsfb Save RI Sensorgram 19020 15980 15940 1aHHI 1aaBll 1a gl Time 2 2 AV Sd or NHS EDC NHS EDC 1 1 Command Injiect Biacor
17. Biacore J Instrument Handbook 32 9 BIACORE J Control Snftware CGC Bia UsErs interactinn_hlrl Filg Edit Wiem Cominahd Fn Inns Hglp RU Interaction 26000 adDDD gg ab0bbb TEOOOD ab00 HI EO SD Timse Tool bar Cycle 5 6 Run Stop BIA HHE in nu want to stop the sensorgrarmy gt TEs Nn Yes Standby 5 7 File Close Biacore J Instrument Handbook 33 6 6 1 1 2 2 3 Standby Tools Stdqndby Flow Standby 5ul min
18. Biacore J Instrument Handbook 38 1 i L 5 Biacore J Instrument Handbook H9 3 BR 6 DR 9 F OD HH
19. Regeneration 1 Biacore J Instrument Handbook 26 L Biacore J Instrument Handbook NaC MgCl 10mM Glycine HCI HCI Formic qcid 10mM Glycine NaOH NaOH EDTA EGTA Surfactant P 20 Tween 20 Triton 100 SDS Octylglucoside Acetonitrile DMSO Ethyleneglycol in HBS buffer Ethanol Formamide GuQanidine HCI Urea pH 12 lt 20 pH lt 3M lt 4M pH 1 5 100mM lt 20 lt 100mM lt 0 35M lt 0 35M lt 59 lt 59 lt 0 5 40mM lt 8 lt 50 lt 20 40 lt 5M lt 8M 27 5 1 Run Start atart Hu
20. 2 1 1 100 200ml 0 22um HBS EP 10mM HEPES 0 15M NaCl 3mM EDTA 0 005 Surfactant P20 pH7 4 HBS P 10mM HEPES 0 15M NaCl 0 005 Surfactant P20 pH7 4 HBS N 10mM HEPES 0 15M NaCl pH7 4 Biacore J Instrument Handbook 2 1 3 Start BIA Programs BiacoreJ Control Software
21. Ne 30 5 5 Oe 31 5 6 ii 32 5 7 32 6 hr 33 6 1 ii 33 6 2 33 6 3 34 6 4 ee 34 6 5 34 7 i ee aa 0 a 0s ee 0a 8 aaa 8 88 0 a 0 E8008 Sai 00 8 8 Re ei 35 7 1 coccoecooceoococooeeccocccececcoceccccccccocccceoccococeccococccecocococeeeeee 35 7 2 Wiad cee Ge ieee cee die es eeeeeeeeeeee 35 7 3 36 8 37 1 N 1 gt 30 1 2
22. OK Biacore J Instrument Handbook 31 30 1 Edit Add Report Point Add Report Point Id OK RU Interactinn RU DDO rHHI BII 26000 5600 dO 22000 ET TBOOD EIHI SH 14DDD SE ab00 II EBIIH HH TDD 120D0 140D Time 3 AbsResk 24894 Ma 59402 sjbound Ne 2545ng EE evonenc2n 24878 157 tggs4gl EO sgzaal 74 Le View Reference Line 5 5 su sa Run New Cycle Do wou wark to start a new cycler Yes
23. J Standby Tools Standby Flow 30 OO COE Sensor Chip Maintenance Biacore J Instrument Handbook 7 3 Sanitize 1 BlAdisinfectant solution Sensor Chip Maintenance MilliQ BIA BIAdisinfectqnt solution 1 5 ml 20 ml MiliO Change Buffer Tools Change Buffer MiliO 15 ml Change Buffer Tools Change Buffer MiliO Standby Tools Stdandby Flow 30 Undock Command Undock Sensor Chip Maintenance Sanitize Desorb
24. Resulsfb Mu Nehwark F Save in C Bia Users File name Save C Bia Users RU Sensorgram TH 1 1 1 1 1GGgl TIme 3 CT DE Biacore J Instrument Handbook 11 3 2 Command Inject FT x 5ample ImlEchinh Contact hmg minll 1 l Flace the pipette tip In the inection port and Click Start directhn pproximate sample consumptior rH Hsp Careel Contact time 100 Stqrt Inject start
25. 96 96 standby We 100ml standby Command Stop Flow MilliO MiliO 6 2 2 1 Dock MiliQ Tools Change Buffer MiliO Change Buffer MiliO Tools Empty Flow System 6 2 6 3 6 4 6 2 Command Undock ET Lindock Ma
26. Research Grade 15 Certified Grade 5 Sensor Chip CM4 CM5 Sensor Chip CM3 CM5 Sensor Chip C1 CM3 NY Sensor Chip SA DNA Sensor Chip NTA
27. BIAmaintenance Kit BIAtest solution 10ul RU Sehsordgram ml uml II al IU gal II 5 THU 1 a I 5 ll ll ll TIme 3 Biacore J Instrument Handbook 12 3 3 RUUI a View Reference Line RU Sensorgram SHIHHII SDL ODO 3 DOL NN OO TSO TUDO D TO 2 300 DID Sl BEDD TL BI dn TOD Time UI 60 0 UN TNINIU 00 0 UM 0 R ESPN Fs Edit Add report point Eport point Fieport Foint Time az baseline Baseline ves Lancel Add report point Id OK
28. sa BIA HHRE JEControl Goftware File Edit ig Command Fun Toos Help Eee 2 IE Ewents Status Time Infn Flow rate Standby Temperature 25 0 C Ewents and Status Menu bar Biacore Tool bar 6 Sensorgram window Report point table Eventlog window Sensorgram window Event mark 4 Stqtus window Biacore J Instrument Handbook 3 2 2 1 Dock 2 Biacore J Instrument Handbook 4 Sensor chip Menu bar
29. pH p 1 2 7 pH 7 50mM 100 ug ml 10mM pH8 5 10mM disodium tetraborate pH8 5 1M NaCl Biacore J Instrument Handbook 18 7 2 pH p pH pH
30. Biacore J Instrument Handbook 29 5 2 Command Inject FT x 5ample ImlEchinh Contact hmg minll 1 l Flace the pipette tip In the inection port and Click Start directhn pproximate sample consumptior rH Lamcel 2 5 Stqart RI Interaction RU 28000 66b0 EAI 24HHH 22000 Pi 2nnnm En 18000 58D0 100 200 300 400 500 BI Th Time 5 3 Command Regenerate EIC zx Hgngehgralinn Contact img 1 mn 100 Flace the pipette tip In the inection port and click Start directh hrngimate sample cornsumptior Me cao 1 EC CY 9lorE Biacore J Instrument Handbook 30 Fl IhteractIQh RI gl ll iti
31. 30 0 90 2 Dual Channel High Flow BIAtest solution 2 30 0 90 Desorb Sanitize Sensor Chip CM5 YY Single Channel Dual Channel 2 020 AI ot a Event mark 4 5 10 22 500RU 1 000RU Event mark 3
32. View Reference Line Biacore J Instrument Handbook 24 RU ImmoBilization of Imouse I HHII 350DD0 LOO ao0bD0 OO 150DD0 D 20D0 HH EL LL 1D000 1200 1 HH 1600 Tinne R PSP PE Baseline a Rg RiNesn 3 tdslimmeb he zisgl 555e 1000RU 1ng mm2z2 RU 5933 6RU 5 9336ng mmz 1 25 mm 1000RU 1ng mm lt Run Stop BIA HHE in wou want to stop the sehsorgram TEs No Yes Standby 4 4 File Close Biacore J Instrument Handbook 25 5
33. Command Dock Linck Insert a sensor chip Llick nn hnck Hgmalnimn time 350 Dock The sustem IE how ready for use 4 OK 2 Dock Undock Dock Sensor chip Biacore J Instrument Handbook 5 3 Sensor Chip CM5 NN 0
34. stqart RI Immoblllzatloh of mouse Id nm UL da JUUUU so0UU ll 15000 1 I I hb dl ll TN 1 4 TE Time 3 FR Biacore J Instrument Handbook 23 4 3 NHS EDC View Reference Line NHS EDC 20 Edit Add Report Point dd rEnnrt nnint Hannrt Foint Time lz ld lkaseind Help Lance Add report point Id Baseline Yes OK eR Easeline lt 30 60 Edit Add Report Point Add Report Point Id OK 9
35. NTA His Tag Fusion Protein Ni Sensor Chip HPA C18 Monolayer Sensor Chip L1 Bilayer Biacore J Instrument Handbook 6 cr 2 2 20 37 Command Set Temperature satel Temperature x as TeEmperature elt temperature Help Lance Status window
36. GE Healthcare Biacore Blacore J Instrument Handbook GE imagination at work 1 IF 1 11 meister 1 1 2 ee 1 1 3 ee 2 2 ii 3 2 1 Dock 3 2 2 PF 6 23 6 3 ii 9 3 1 ee 9 3 2 11 3 3 ere 12 3 4 ii 14 3 5 14 4 15 9 i i iI NC 16 42 oro 19 4 3 oro 23 44 24 5 25 5 1 27 5 2 29 5 3 29 5 4
37. e J Instrument Handbook 21 lnect 6 NHS EDC start Ll Sensorgram al NN NN NN 14LHIH ml 1 150 IN ga Jl Jul II 30 ml Sal TIme 3 10 100 ug ml 10mM 7 Command Iniect lnect 6 MN CCV SR VT Biacore J Instrument Handbook 22 RI Immoblllzatioh of mouse Id al 30000 25000 20000 15000 1 a JI FLL dl THON TLL Time 3 Command Inject ample IIEChinh Contact hime mm 1 Place the pipette hp i the Iachinh port and click Start directly PproIimate sample corsumptior 190 EI He Cara 6 1 M Ethanolamine hydrochloride
38. ke sure that hie is ho pipethe hp in the ieclion hot ad cick on Undock Mral uFMil th Oecdte EE Firished behere leTmwin me sarrot chip Undock Undock Undock Sensor chip Dock Biacore J Instrument Handbook 34 3 P 33 6 1 2 3 Standby 6 4 File Close File gt Exit 6 5 4 50ml 4
39. lt 6600 5 gn blU 3 ol 1 bil 1 Al ii ho Hl 1000 1200 1400 Time 5 4 3 View Reference Line 10 20 Edit Add Report Point dd rEnnrt point x lz baselna Easeline es Hep Cancel Add Report Point Id boseline Baqseline OK 10 20 Edit Add Report Point Add Report Point Id
40. n Analysis mnde Make sure that the tubings nn the conmector hlnrk are COnnmected as shown IF nnu change the uhin Conmnections Click Carcel PrImE the How snstem start the sensorogram Next gt artart Hun Flow rate Medium Start Biacore J Instrument Handbook 28 Tare Results As Sawe i Training TT i F FP ET Ella hn Documents MLComputer m E File mame WE as ug Resuls blr Lance 2 Save RU Interaction RU 25400 24200 09 23000 5346 21800 944 20600 534 lt wg 19400 5940 18200 5938 17nnn 5936 1n 32n 30 In 50 ED 7 BD qn 100 Tirme 2000 8 Dual channel Reference subtraction Fc1 Fc2 Fc1 Fc2 Response Resp Diff
41. re J Instrument Handbook 8 4 2 100 250Hl Injection port type2 1000H Injection port type3 Biacore J Instrument Handbook 9 3 3 1 ka Run Start mntart Hun Analysis mode Single Channel Next gt Single Channel 1 Dual channel 2 mstart Hun Tubing connections Make sure that the tubings nn the conmector hlnrk are COnnmected as shown IF nnu change the uhin connections Click Carcel PrIme the In snstEm Fstarl the ssnr Ta Next gt artart Hun Flow rate Low Medium High Start Biacore J Instrument Handbook 10 Tare Results As ET OE Ella skhn p
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