Bio-Scale CHT-I カラム 取扱説明書 - Bio-Rad
Contents
1. 3 3 3 3 1 2 22. pH NaCl Table 1 CHT CHT2 I CHT5 I CHT10 CHT20 I Column volume ml Recommended max protein 20 50 100 200 loading mg Recommended flow rate 0 5 3 0 0 5 5 0 0 5 7 0 0 5 10 0 ml min Dynamic binding capacity 30 75 150 300 mg Lysozyme Column Static DNA binding capacity mg calf thymus DNA Average particle size um 10 3 0 Nominal pore diameter A 600 800 Column dimension mm 10 x 64 12 x 88 15 x 113 Maximum operating pressure 1000 6 7 67 750 5 0 50 600 4 0 40 500 3 4 34 psi Mpa bar
3. 1 ml 10 ml 20 ml CHT 2 3 2 4
4. 7 8 2ml min 5 10 3 4 Top Off Resin Top Off Resin mm
5. Fig 3 Fig 3 lt gt 1 2 3 4 5 6 5ml
6. 2ml 5ml 10ml 20ml 4 1 2 CHT Type I CHT Type I CHT 1 pl pl Ca PO
7. 1 3 gt Table 2 2 3
8. PEEK CHT I gt CHT I pH 5 5 14 2M NaOH 6 M 8 M Triton X 100 CHAPS CTAB 1 SDS EDTA EGTA CHT Tris Tris CHT
9. 4 C SDS Table 2 CHT 2M EDTA 8M 6M Tris 100 Triton X 100 CHAPS CTAB 100 1 SDS 2 Bio Scale CHT 2 1 1 4 28 BioLogic DuoFlow
10. pH 7 0 7 0 3x10 M pH 7 0 10 mM 500 mM 10 mM 0 3 mM 500 M 0 00 6mM pH 6 0 3 x 10 M pH 8 0 3x107 M 2 6 2 1
11. 4 1 2 2 2 0 2 1 3 1 4x28 4 M6 2 2 5 10 32 2 2 Fig 1 Bio Scale CHT I 20 5 mM pH 6 8 HPLC 0 2 0 45 ux m 50 Table 1 gt 1 5
12. pH NaCI pH gt Bio Scale CHT2 48 ml Fig 2 2 0 m min 6 ml 3 0 5 M 24 ml 12 6 ml 3 12 ml 6 100 m 5 90 m 0 6 12 18 24 30 36 42 48 Volume ml Fig 2 CHT2
13. 2 2 M6 2 2 2 2 0 2 1 751 0023 Bio Scale CHT5 I 5ml 1 4 x 28 1 4 x 28 2 10 32 2 2 M6 2 2 2 2 0 2 1 751 0025 Bio Scale CHT10 I 10ml 1 4 x 28 1 4 x 28 2 10 32 2 2 M6 2 2 2 2 0 2 1 751 0027 Bio Scale CHT20 I 20ml 1 4 x 28 1 4x28 8 2 10 32 2 2
14. 3 5 3 20 5 mM pH 6 8 0 02 0 05 5 mM pH 6 8 3 6 1 Guide to Sample Preparation Bio Rad Laboratories Technical Bulletin 1322 2 Hydroxylapatite T L Brooks Calbiochem Biochemicals 3 HPLC of Proteins on an Hydroxyapatite Column T Kadoya et al 1988 J Liguid Chromatography 11 No 14 2951 2967 4 Ceramic Hydroxyapatite HPLC of Complexes Membrane Protein and Sodium Dodecy Sulfate T Horigome et al 1989 European Journal of Biochemistry 186 63 69 5 A Guide to the Properties and Uses of Detergents in Biology and Biochemistry J ege ge Calbiochem Biochemicals 4 Product Information Hn ny 751 0021 Bio Scale CHT2 I 2ml 1 4 x 28 1 4 x 28 2 10 32
15. UV 3 Bio Scale CHT 3 1 0 2 0 45 x m 0 5 M Table 1 25 1 2 2 0 M NaCl KCI 3
16. Bio Scale Table 2 SDS UV Critical micelle concentration CMC CHT I 1 4 5 2 5
17. 140 0002 2 2 24 20 Te 03 6361 7000 Fax 03 5463 8480 532 0025 1 14 11 Te 06 6308 6568 ax 06 6308 3064 812 0013 2 5 28 Tel 092 475 4856 ax 092 474 4858 Tel 03 6404 0331 Fax 03 6404 0334 E Mail life ps jp bio rad com M2510 1306A
18. 5 10 mM pH 6 8 2 1 2 0 M NaOH 3 3 3 1 3 2 0 5M 3 6M 8M 3 2
19. M6 2 2 2 2 0 2 1 751 0029 Top off Resin CHT CHT 1ml 5 5 0 2 1 751 0091 Bio Scale 2 5 5 O 2 1 751 0093 Bio Scale 5 5 5 0 2 1 751 0095 Bio Scale 10 5 5 O 2 1 751 0097 Bio Scale 20 750 0561 Tefzel 1 4 28 M6 2 750 0561HPL 1 4 28 10 32 2 750 0564 BioLogic HPLC
20. 2 5 5 10 mM pH6 8 3 5 500 mM pH 6 8 4 5 2 2 pH 6 30 10DG P 6DG P6 0 2 0 45m
21. Bio Scale CHT I 751 0021 751 0023 751 0025 751 0027 06Sealen6HII RM 1 RSS 1 1 2 CHT Type 1 1 2 Bio Scale CHT 3 SICPRNYRGONOS2RRSMRCOMB 3 2 2 URBANR203885302NRE22RHESRSRS2 3 9nr SR 4 2 4 LE 5 2 5 SES SL 5 2 6 SS RE 5 3 Bio Scale CHT 6 90 RE83RCGGRRR882RHSRRERE0EGSSSOSaoK 6 832S 6 S95 TYED NON Steg 6 3 4 Top Off Resin 8 385 SS 8 6 8 4 Product Information 9 1 Bio Scale CHT I 1 1 Bio Scale CHT CHT Type 10um
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