取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. 20 30mer GC 40 60 200bp DNA RNase free RNase free DEPC DEPC2. PCR PCR GC RNase RNA
3. Temperature Transition Rate 20 C sec 2 C sec 11 5 Total RNA DNase AGPC Acid Guanidium Phenol Chloroform Total RNA DNA DNA 1 Distilled Water RNase free 9rade up to 10 HI Total RNA X Hg 10x DNase Buffer 1 HI RNase free DNase 10U ul 0 5 Hl Total volume 10 Hl 2 10 30 100ul Distilled Water 100ul TE 5
4. Mn OAc 2 5mM RNA Mn OAc RNA Total RNA 1ug poly A RNA mRNA 200ng 10 2 PCR RT PCR Denature 1 90 C 0 30 61 C 20 00 2 95 C 0 30 Cycle 95 C 0 00 45 Cycles 60 C 0 45 data collection p 2 Denature 1 90 C 30 RNA
5. RNA Total RNA 2 5ug poly A RNA mRNA 500ng 2 PCR RT PCR Denature 1 90 C 0 30 61 C 20 00 2 95 C 1 00 Cycle 95 C 0 15 45 Cycles 60 C 0 60 data collection p 2 Denature 1 90 C 30 RNA Applied Biosystems 7900HT Standard 3 LightCycler Roche Diagnostics TaqMan 1
6. 2 4 C 50mM Mn OAc 1 20 volume 2 5mM RNA 20 C 3 PCR LineGene BioFlux Applied Biosystems Realtime PCR LightCycler Roche Diagnostics PCR
7. RNase free DEPC DEPC DEPC PCR RNase free 10 30 pmol 0 2 0 6uM TaqMan 2 5 15 pmol 0 05 0 3uM Mn OAc 2 5mM RNA Mn OAc
8. DEPC PCR RNase free Total RNA Total RNA AGPC Acid Guanidium Phenol Chloroform Total RNA DNA DNA DNase DNA Total RNA mRNA 1 2 poly A RNA mRNA poly A RNA oligo dT polyA mRNA mRNA mRNA
9. Denature 1 90 C 30 RNA BioFlux LineGene 33 0 2ul PCR 10ul 2 Applied Biosystems 7900HT TaqMan 1 Distilled Water RNase free 9rade up to 50 HI RNA4 airecty Realtime PCR Master Mix 25 ul 50mM Mn OAc gt 2 5 ul 2 5mM Sense Primer 15 pmol 0 3uM Antisense Primer 15 pmol 0 3uM TaqMan Probe 10 pmol 0 2uM RNA sample lt 2 5ug Total volume 50 Hl Distilled Water RNase free RNase free lt
10. RNA RNA DNA DNA DNase DNA 13 2 PCR PCR PCR
11. rT7th DNA Polymerase 2 Mn 1 PCR 2x PCR 1 MA g77ec7 Realtime PCR Master Mix TaqMan FRET PCR 1 PCR RNA direct Realtime PCR Master Mix RNA PCR 2 RNA
12. 12 a 5 500m 5 5ul 20mg ml 100ul 5M 200ul 20 C 30 a 5 70 12 i 5 Distilled Water 2 6 1 data collection data collection
13. GC PCR 3 PCR 14 7 One step Realtime PCR One step Realtime PCR SYBR Green 500ulx5 QRT 201 RNA direct SYBR Green 500ul x 5 x5 QRT 201 x5 Realtime PCR Master Mix RNA Tota
14. Total RNA RNase 4 1 LineGene BioFlux TaqMan 1 Distilled Water RNase free grade up to 10 HI RNA direct Realtime PCR Master Mix 5 Ll 50mM Mn OAc gt 0 5 ul 2 5mM Sense Primer 3 pmol 0 3uM Antisense Primer 3 pmol 0 3uM TaqMan Probe 2 pmol 0 2uM RNA sample lt 500ng Total volume 10 HI Distilled Water RNase free RNase free lt RNase free DEPC DEPC DEPC PCR RNase free 2 6 pmol 0 2 0 6uM TaqMan 0 5 3 pmol 0 05
15. GC rich RNA direct Realtime PCR Master Mix PCR rTth DNA Polymerase RNA rT7th DNA Polymerase GC rich PCR 3 RNA direct Realtime PCR Master Mix DNA RNA 4 RNA direct Realtime PCR Master Mix LineGene BioFlux Ligh
16. Distilled Water KNase free grade up to 20 HI RNA4 airecty Realtime PCR Master Mix 10 ul 50mM Mn OAc gt 1 HI 2 5mM Sense Primer 6 pmol 0 3uM Antisense Primer 6 pmol 0 3uM TaqMan Probe 4 pmol 0 2uM RNA sample lt 1Hg Total volume 20 Hl Distilled Water RNase free RNase free lt RNase free DEPC DEPC DEPC PCR RNase free 4 12 pmol 0 2 0 6uM TaqMan 1 6 pmol 0 05 0 3uM
17. and claims outside the US corresponding to US Patent No 4 889 818 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research Separate purchase of a Licensed Probe would convey rights under the applicable claims of US Patents Nos 5 538 848 5 723 591 5 876 930 6 030 787 6 258 569 5 804 375 claims 1 12 only and 6 214 979 and corresponding claims outside the United States No right under any other patent claim and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchasing licenses may be obtained from the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA LightCycler Idaho Technology Inc Roche Molecular Systems Inc TaqMan Roche Molecular Systems Inc SYBR Molecular Probes Inc 1 RNA direct Realtime PCR Master Mix Thermus thermophilus HB8 DNA
18. 0 3uM Mn OAc gt 2 5mM RNA Mn OAc RNA Total RNA 500ng poly A RNA mRNA 100ng 2 PCR RT PCR Denature 1 90 C 0 30 61 C 20 00 2 95 C 0 30 06 Cycle 95 C 0 15 45 Cycles 60 C 0 45 data collection p 2
19. TOYOBO MM ldeas amp Chemistry One step qPCR Kit RNA direct Realtime PCR Master Mix Code No QRT 101 QRT 101X5 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3586K 1 2 3 5 6 4 7 2 C7 NOTICE TO PURCHASER LIMITED LICENSE A license to perform the patented 5 Nuclease Process for research is obtained by the purchase of i both Authorized 5 Nuclease Core Kit and Licensed Probe ii a Licensed 5 Nuclease Kit or iii license rights from Applied Biosystems This product is an Authorized 5 Nuclease Core Kit Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 079 352 5 789 224 5 618 7 11 6 127 155 5 677 152 5 773 258 5 407 800 5 322 770 5 310 652 5 210 015 5 487 972
20. ix with gDNA Remover ReverTra Ace SYBRY Green PCR THUNDERBIRD SYBR qPCR Mix 10 000U x 1 TRT 101 10 000U x 1 x 5 TRT 101X5 10 000U x 1 x 10 TRT 101X10 DNA RNase 2 500U 1 SIN 201 RNase Inhibitor Recombinant 2 500U x 1 x 5 SIN 201X5 15 HPCR 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
21. l RNA MagExtractor RNA NPK 201F Magical Trapper MGS 101 PCR PCR 1mlx5 QPK 101 Realtime PCR Master Mix 1mlx5 x5 QPK 101X5 I PCR 1mlx5 QPK 201 SYBR Green 1mlx5 x5 QPK 201X5 SYBR Green Realtime PCR Master Mix PCR 1ml x 5 QPK 212 SYBR Green SYBR Green Realtime PCR Master Mix Plus 1mlX5 X5 QRIN2I2AS 1mlx 1 QPS 101T PCR 1 67ml x 3 QPS 101 THUNDERBIRDY Probe qPCR Mix 1 67mlx 3 x5 QPS 101X5 1mlx 1 QPS 201T 1 67mlx 3 QPS 201 1 67mlx 3 x 5 QPS 201X5 PCR cDNA ReverTra Ace qPCR RT Kit 200 FSQ 101 PCR cDNA ReverTra Ace qPCR RT Master Mix 200 FSQ 201 DNA PCR cDNA 200 FSQ 301 ReverTra Ace qPCR RT Master M
22. tCycler Roche Diagnostics passive reference Applied Biosystems Realtime PCR PCR 2 QRT 101 50ul QRT 101X5 x 100 20 C RNA direct 4 C BONE Realtime PCR Master Mix 2 QRT 101 x 5 50mM Mn OAc gt 20 C 500ul x 1 RNA direct Realtime PCR Master Mix dNTPs r7th DNA DNA PCR passive reference 2x RNA Mn OAc RNase free 1x 20 C 20 C 10
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