Transdirect insect cell 取扱い説明書
Contents
1. A N N RI RI RI MSDS SHIMADZU 11I BIOTECH kkRI Rh i A Al iti i 12. SS 12 1 5 SHIMADZU 13 BIOTEC CH RI RI SDS PAGE 7 SDS PAGE CBB 2 CBB 3 X 6 1 Amersham Biosciences Amplify Fluorographic Reagents Code No NAMP100 80 C Promega FluoroTectrM Green im vitro Trans
3. 5 50 L mRNA 16 ug Reaction Buffer 15 L Insect Cell Extract 25 uL 4 mM Methionine 1 L 50 L SHIMADZU 11 BIOTEC CH 4 C 1 Insect Cell Extract 80 C 2 30 80 Reaction Buffer 4 mM Methionine mRNA 25 C
4. mRNA Sf21 Spodoptera geygg Malacosma neustria nucleopolyhedrovirus 5 P N 292 30000 91 1 P N 292 30011 1 50 gL 40 Insect Cell Extract 210 LI 5 80 C 19 Reaction Buffer 630 L 1 80
5. 5 C 5 2 1 PCR cDNA PCR 2 PCR Fidelity Terminal transferase PCR KODplus Code No KOD 201 N 5 ATG 3 ATG TGA ORF ACT 3 ACT CTTAAG GG 5 co RI C
6. Insect Cell Extract Reaction Buffer 80 C 4 mM Methionine 0 5 g L pTD1 Vector 0 5 g L Control DNA 20 C EF 1 mRNA RNase mRNA RNase inhibitor Code No 2310A Promega Code No N2611 50 L 50 units mRNA mRNA mRNA mRNA
7. T7 RNA polymerase promoter 240 258 5UR cs SUTR PoyAemmnawr Polyhedrin 5 untranslated region 259 304 Multiple cloning region 305 343 3 Untranslated region 359 731 PolyA region 736 757 T7 transcription terminator 758 805 ColE1 ori 835 1104 8B lactamase Amp coding region 1992 2852 pTD1 Vector 3052 bp 2 pTD1 Vector 4 MA BIOTEC CH GCAGATTGTA CTGAGAGTGC _ ACCATATGCG GTGTGAAATA _ CCGCACAGAT GCGTAAGGAG 220 Forward primer AAAATACCGC ATCAGGCC AATACGACTC ACTATAGGAG TATTTTTATT CTTTCGTAAA 280 T7 Promoter Polyhedrin PUTR S773 1 ET AAAATTAGAA AAATAAAATA TAAAGATATC GAATTCGAGC TCGGTACCCG _ GGGATCCTCT 340 i Eco RV coRI Sacl Kpnl BamH Xbal AGAGTCGGGC GGCTGTAAAA CACGATACAT TGTTATTAGT ACATTTATTA AGCGCTAGAT 400 TCTGTGCGTT GTTGATTTAC 420 Reverse primer 3 pTD1 Vector pTD1 Vector 5UTR
8. 1 2 N pTD1 4 PCR 7 DNA mRNA IES 8 mRNA i mRNA IS 8 i Transdirect sec cel ES 11 2 1220 P N 292 30000 92 20 20 292 30000 93 50 50 292 30000 94 100 100 SHIMADZU 3 BIOTEC CH In vitro DNA 7 vtro DNA pTD1 Vector pTD1 Vector
9. SHIMADZU 17 BIOTEC CH mRNA NTPs mRNA mRNA LS 8 8 80 C FW 2 cDNA ORF Eco RV SW 5 0 5 ug uL pTD1 Vector RI DEBOD 0
10. 4 mM Methionine 50 L 1 20 10 5 ug uL pTD1 Vector 10 L 1 20 C 8 05 g L Control DNA 10 pL 1 20 C pTD1 DNA Insect Cell Extract Reaction Buffer 80 C 4 mM Methionine 0 5 g L pTD1 Vector 0 5 g L Control DNA 20 80 S Methionine RI Reaction Buffer SHIMADZU BIOTEC CH Insect Cell Extract 1 Lot No 2 Lot
11. 5UTR gco RV 5UTR PCR 5 UTR 3 DNA gp PCR SHIMADZU 5 BIOTEC CH In vitro DNA gco RV 7 ORF PCR N C
12. PCR DNA 50 1 N 5 GCAGATTGTACTGAGAGTG 3 161 179 2 M13 C 5 GGAAACAGCTATGACCATG 3 845 827 3 pTD1Vector C 101 2145 oe 1 183 co 521 806 ozI 805 co T141 769 w I 994 Hind ll 813 Scal 2543 Stul 234 SHIMADZU 7 BIOTEC CH mRNA mRNA 37 mRNA mRNA 4 mRNA
13. 3 Insect Cell Extract COz CO gt Lot No Insect Cell Extract 8 80 25 37 mRNA T7 7 or 1 TE 3M pH5 5 TE se IX Xx 70 2 SHIMADZU BIOTEC CH
14. Biospec mini 8B 7 2 Promega 10 gL 1 x Reporter Lysis Buffer 490 L 50 50 10 uL 1 x Reporter Lysis Buffer 140 L Assay 2 Xx Buffer 150 gL 37 30 30 1M Sodium Carbonate 500 L 420 nm 50 U mL BB 10 U mL 8 SHIMADZU 15 BIOTEC CH
15. 3 mL mRNA 100 xL o gt Cm CS Nh 400 L SHIMADZU 9 BIOTEC CH mRNA 7 mRNA 1 5 mL 8 400 L mRNA 400 L 9 mRNA 40 L 950 uxL 15 000 rpm 4 C 20 70 70 mRNA 20 100 L mRNA 2 g L 7 mRNA 400 wL 2 9 9 10
16. 10 BIOTEC CH 8 MM 3M pH 5 5 2 70 120 C 20 w 1 GE Healthcare NICK Columns Code No 17 0855 01 2 NICK Columns mRNA GE Healthcare 3 mL
17. X Gal 20 mg mL X 3 LL X Gal 1 L 16 L Total 20 uL 7 37 C 20 2 mRNA 16 MIN BIOTEC CH Hn DH5 a Invitrogen Code No 18258 012 LB mRNA DNA 70 9 ST 7 mRNA DNA RNase
18. 4 PCR PCR PCR pTD1 Vector Eco RV CS hoO 5 pTD1 Vector 6 SHIMADZ BIOTECH 50 N 5 GCAGATTGTACTGAGAGTG 3 161 179 C 5 ACAACGCACAGAATCTAGC 3 412 394 mRNA DNA kn DNA ind oz 1 RNase
19. J EO 2 TRd 3 RI RI 18 REY B IOTECH 2004 42 639 641 2005 83 390 391 Ezure T et al Cell free protein synthesis system prepared from insect cells by freeze thawing Biotechnol Prog 2006 22 1570 1577 Suzuki T et al Performance of expression vector pTD1 in insect cel free translation system J
20. Scale RNA Production System Code No AmpliScribe 4 T7 fFlash Transcription Kit Epicentre ASF3257 AmpliScribe 1 T7 Transcription Kit Epicentre AS3107 3 EE 304 14641 Transcription Kit MEGA script T7 Ha Yield Ta CS hor 1334 RiboMAX Large Scale RNA Production System T7 Promega P1300 RNAMaxx Yield TansGHbtSr Ki 200339 ScriptMAXTM 2 Thermo T7 Transcription Kit TSK101 HN TM T7 RiboMAX Express Large Promega P1320 mRNA mRNA 2 NTPs A B NTPs mRNA mR A A SS 9 B
21. A 2 pTD1 Vector mRNA F 5 UTR NNN 50 100 g mL plD1 Vector DNA DDBJ GenBank EMBL Accession Number AB194742 pTD1 Vector pTD1 Vector pTD1 5UTR mRNA 2 pTD1 Vector
22. 3 1 II 2004 2008 Shimadzu Corporation All rights reserved 1 SHIMADZU BIOT
23. SS NS SS 1 1 Insect Cell Extract He 2 2 3905 ROSEHRSRSSOSRSSRSSRPRGRSEPRRSCRSRRGNSSSIHRZYRNs 2 2 II 2 ot 3 3 4 vitro DNA LE EL 4 pTDIMecto ER E mRNA DNA Ti mRNA SEASSSSESSOSSSOAAA ii mRNA mRNA eees SAA RI ee 14 14 B 14 lt ki 15 YY 17 17 SS 19 MSDS 19 RSS NR 19 IV TsHIMADZU BIOTECH Transdirect secr ce
24. NTPs mRNA mRNA mRNA TE 100 400 TE mRNA wg L Azeo x 0 04 x BIOTEC CH 10 RGAEDIN RI 121 C 20 25 mRNA RI 5S Methionine Promega FluoroTect Greeni in vitro Translation Labeling System Code No L5001 50 gL
25. 5 oo wh 12 GAP BIOTECH S Methionine Promega FluoroTect Green in vitro Translation Labeling System Code No L5001 50 zxL RI 3H Leucine 4 mM Methionine 6 50 xuL S Met FluoroTect W mRNA 16 ug 16 ug Reaction Buffer 15 L 15 L Insect Cell Extract 25 UL 25 L 3 S Methionine 1200 Ci mmoL 10 mCirmL eR 4 mM Methionine 1 L FluoroTect Green tRNA 1 L 50 L RI RI RI
26. 19 BIOTEC CH
27. 292 30011D Nov 2008 sHIMADzZUU BioTEcH Transdirect insect cell Na H i 1 2
28. Biosci Bioeng 2006 102 69 71 Suzuki T et al N terminal protein modifications in an insect cel free protein synthesis system and their identification by mass spectrometry Proteomics 2006 6 4486 4495 Suzuki T et al Preparation of N acylated proteins modified with fatty acids having a specific chain length using an insect cell free protein synthesis system Biosci Biotechnol Biochem 2007 71 261 264 Suzuki T ef g Protein prenylation in an insect cell free protein synthesis system and identification of products by mass spectrometry Proteomics 2007 7 1942 1950 Ezure T et al Expression of proteins containing disulfide bonds in an insect cell free system and confirmation of their arrangements by MALDI TOF mass spectrometry Proteomics 2007 7 4424 4434 MSDS MSDS Web 604 8511 1 TEL 075 823 1351 FAX 075 823 1364 E MAIL t direct shimadzu biotechjp http www shimadzu biotechjp SHIMADZU
29. D iz 7 3 oie SR 8 0
30. ECH N
31. lation Labeling System Code No L5001 SDS PAGE 0 5 ug gL Control DNA 8B DNA 7 0 5 g L Control DNA mRNA LS 7 8 mRNA 2 8 mRNA ES 12 BIOTEC CH 14 962 86 Promega 8 Galactosidase Enzyme Assay System with Reporter Lysis Buffer Code No E2000
Download Pdf Manuals
Related Search