KOD －Plus - 東洋紡ライフサイエンス事業部
Contents
1. KOD Plus M 1 2 3 4 5 6 M A Hind II Marker KOD Plus DNA 50ng KOD Plus DNA 100ng A DNA 50ng A DNA 100ng B DNA 50ng B DNA 100ng o On ROD 2 TGF GC 70 DMSO DNA TGF GC 709 2kb 30 PCR DMSO DMSO KOD DNA Polymerase M 1 2 3 M 1kb 1 KOD Plus 2 KOD Plus 292DMSO 3 KOD Plus 592DMSO gt TOYOBO2. 2 5 DNA MgSO 1 0mM 0 8mM 1U 0 5 0 8U TA TArget Clone Plus 10x Buffer for KOD Plus gt TEL 06 6348 3888 9 00 12 00 13 00 1 E mail tech_osaka toyobo jp FAX 06 6348 3833 7 00 URL http 7www toyobo co jp bio 7 1 DNA Polymerase p53 4kp PCR PCR DNA Polymerase DNA 50 100ng
3. PCR 1 KOD Plus 3 KOD 201 KOD 201X5 KOD 201X10 200Ux1 KOD Plus 1 0 U gl 200lx1 10x Buffer for KOD Plus 1mlx 1 KOD 201 x5 KOD 201 x10 25mM MgSO4 1mlx1 2mM dNTPs 1mlx 1 2 3 1 KOD Plus PCR 50 1I 1U KOD Plus 1U ADNA 21kb Genomic D
4. RNA 100ng Total RNA1 rg 20 PCR 2l DNA RNA RNA DNA DNA RNase 9 30 40 10 PCR 11 GC rich DMSO 2 lt 592 PCR DNA DNA 50 200ng Colony Direct PCR PCR PCR blunt end PCR
5. TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobo jp URL http 7www toyobo co jp bio htlp wwwitoyobo co jp bio 1 PCR 2 KOD Plus Site Directed Mutagenesis 3 KOD Plus Long PCR 4 PCR 5 Colony DirectPCR 6 Colony DirectPCR KOD Plus 8 Code No lt M MLV gt 10 000 Ux1 TRT 101 ReverTra Ace 100 U gl 10 000
6. GC 22 34mer Tm gt 60C PCR Tm 65C DNA PCR DNA DNA RNA PCR gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http 7www toyobo co jp bio 7 DNA RNA PCR PCR 50 Il
7. blunt end KOD KOD Plus TA TArget Clone Plus TA 6 1 0mM 1 2mM MgSO 1 5 2 0mM Tm 10 C 2 5 DNA DNA RNA RNA RNA 68 PCR MgSQ 1 0mM 0 8mM
8. E mail tech_osaka toyobo jp URL http 7www toyobo co jp bio 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order lifescience toyobo jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order lifescience toyobo jp gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobo jp URL http 7www toyobo co jp bio
9. Plus 1U 1 gl 1U650l Autoclaved distilled water to 50 gl 2 PCR 3 Tm 72 C PCR 2 gt 3 6 Pre Denature 94 C 2min Pre Denature 94C 2min Denature 94 C 15sec Denature 94 C 15sec 25 35 Annealing Tm 5 C 30sec a Extension 68 C 1min kb cycles Extension 68 C 1min kb Extension 1kb 1min 5 1 2 3 4 5 6 thin wall dNTPs dNTPs Mixture 2mM Code No NTP 201
10. Ux1 x5 TRT 101X5 10 000 Ux1 x10 TRT 101X10 lt PCR gt 250 Ux1 KOD 101 KOD DNA Polymerase 250 Ux1 x5 KOD 101X5 lt PCR gt 200Ux1 KOD 211 KOD Plus Ver2 200 Ux1 x5 KOD 211X5 lt PCR gt 200Ux1 KOD 401 KOD Plus Neo 200 Ux1 x5 KOD 401X5 200 Ux1 x10 KOD 401X10 lt KOD KOD Plus TA gt TArget Clone Plus MM IN dNTPs Mixture 2mM 1 ml NTP 201 KOD Plus Buffer 1 ml KOD 2B KOD Plus MgSO4 1ml KOD 2S KOD Plus Ver2 Buffer 1ml KOD 3B KOD Plus Neo Buffer 1 ml KOD 4B 1 Takagi M Nishioka M Kakihara H Kitabayashi M Inoue H Kawakami B Oka M and Imanaka T App Environ Microbiol 63 4504 4510 1997 2 Hashimoto H Matsumoto T Nishioka M Yuasa 1 Takeuchi S Inoue T Fujiwara S Takadi M Imanaka T and Kai Y J Biochem Tokyo 125 983 986 1999 3 Mizuguchi H Nakatsuji M Fujiwara S Takagi M and Imanaka T J Biochem Tokyo 126 762 768 1999 gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 17 00
11. 12 11 Code No KOD 201 Code No KOD 201X5 Code No KOD 201X10 20C P CR KOD Plus KOD Plus 7 er77ococcus kodakaranesis KOD1 KOD DNA Polymerase PCR KOD DNA Polymerase 2 Hot start PCR KOD DNA Polymerase Polymerase 3 5 Exonuclease Proofreading PCR KOD DNA Polymerase Taq DNA Polymerase 82 KOD DNA Polymerase 2 KOD Polymerase Exonuclease PCR
12. NA 12kb 7kb 2 KOD Plus Human globin 3 6kb Human p53 4 0kb gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobo jp A3616K URL http 7www toyobo co jp bio HPCR a 1 4 PCR 1 PCR 10x PCR Buffer for KOD Plus Buffer Components Volume FinalConcentration 10x PCR Buffer for KOD Plus 5 ul 1x 2mM dNTPs 5 gl 0 2mM each 25mM MgSO4 2 al 1 0mM 10 uM each 1 5 ul 0 3Meach Genomic DNA 10 200 ng 50 gtl DNA gt 1 ul Plasmid DNA 1 50 ng 50 cDNA 5 7 KOD
Download Pdf Manuals
Related Search