ALR-103
Contents
1. Stop solution 37 SDS formamide 50 60 50 60C TGase DNA 566bp2. NBT 1383213 Roche BCIP 1383221 Roche CDP Star 1759051 Roche 1 PCR 400 ng 95C 5 400 ng Buffer 4 Alkaline phosphatase 5uL TGase 2uL DEPC Treated Water to 40 pL PCR 40C DNA 80 RNA 2 Stop solution 4uL TGase Alkaline phosphatase 1 Alkaline phosphatase
3. 6 20
4. DNA Mi 1 0 Q DNA 2 20 Q DNA 3 40 Q DNA 4 60 Q DNA 5 80 Q DNA 20101203 RN1 3763Rev 0 DNA OPCR DNA dUTP dNTP ALR 101 PCR 80 uL QTIA quick PCR Purification kit Qiagen 380 nL 40C 80 Stop solution 4 uL TGase Hybridization Buffer 55 30 Hybridization Buffer 10 ngmL 55 a Buffer 2
5. DNA DNA dUTP dNTP ALR 101 RNA RNA UTP NTP ALR 102 Q dUTP Q d UTP DNA dUTP dNTP ALR 101 RNA UTP NTP ALR 102 TGase ALR 103 TT 5 Alkaline phosphatase 8 mg mL 100 pL TGase 10 U mL 40 pL Buffer 10 x 80 pL DEPC treated Water 1 mL Stop solution 10 x 80 pL 20 20
6. 55C 10 2 We Buffer 9 5 2 1 Hybridization Buffer 2 M Urea 20 mM phosphate buffer pH8 0 0 5 M NaCl 1 mg mL torula yeast RNA 1x Denhardt s solution 1 Casein 0 1 Triton X 100 5 Dextran sulfate MW 500 000 2 Wash 1 Buffer 50 mM phosphate buffer pH8 0 150 mM NaCl 0 1 Triton X 100 3 Wash 2 Buffer 100 mM NaCl 100 mM Tris HC1 pH9 5 50 mM MgCls 0 1 Triton X 100 181 8622 6 22 1 0422 45 5181 http www aloka co jp e mail bio aloka co jp
7. ALOKA Labelling ONE ALR 103 20101203 RN1 3763Rev 0 20C N 1 2 Q TGase K
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