S1000サーマルサイクラー 取扱説明書 - Bio-Rad
Contents
1. 00 00000 00000000000 000000010 51000 0000 0000 HB HELD 000000000000000 00000 000000000 0000 0000000000000 10000000 Ugo BE EE ICI CI 98 10 0 O OL OOOO BOE BE ELI 00000 9000000000000 90 000000000 OOOO 000 000000 0000000000 miu uidi 00090 000000 900000010000 BNE 00000000000 90000 0001001000 OOOO L 000000090 00000 000 00000000000 000002. 2 1 1100 m p D 0000 00000000 09510 Up p 0 0000 0000090000 000000000000 E CON EUC
3. 00 0 00010000 TEMPEEDDODULU U III 00 0 O 0510000 0000000000 HO DEBEO CIO LECT D AL Ha uuu UU DOO D D DEC HE HO D DD D 7E 7E CTI UE L 68 51000 0000000000000 1 0000000 00 00000 000000000 5100000000 00000 000000 0000 000 00000000 000 UD Bd uu 2 PCR 0000000 0 TEMP III I BLU LU
4. COPY O 1 IIENTER I I III DO UI BOE EE BE OI DB DU 7E LOT FOLDERS RENAME REHAME 2 000000 5 E Qm m 1k 3 00000 00000 00 000000000000 0000 000 0 DE 00000 Ul 73 31000 COPY SID TO lt HRIH EUR gt GRBHT Unt 4 0 0000000000 00010 0000 0000 0000000 00000000 00000 000000
5. ui 60 5 53 OOOO lI ED E C 00000 00000000000 Chill out Liquid Vex D p 0000 0005000000 00000 000000000000 0000 0000
6. 33 31000 0 BEEP 000 0000 000 0000000 00 00000 000 0000 40000000000 00000 OPTIONIIII uiid I 000 000 00000 00 02600 0000000 5000 O 0000 00 900 ud uiui 000 00 0000 000000000000 00000 0O UB 9 TEMPI LLEIBU DH LB D D D D DU L Changes the target The range is 10 to 10 C cycle in temperature of step tenths of a degree within the by the specified temperature limits of a temperature increment each cycle step 0 100 A step with an increment must be within a GOTO repeat in order to increment the temperature with each cycle Extends the hold time The range is 60 to 60 seconds cycle by the specified in whole numbers within the time number of seconds limits of a temperature step 1 each cycle second to 18 hours step with an extend must be within a GOTO repeat in order to extend the step with each cycle Changes the ramping The range is 0 1 to 5 rate tenths of a degree for temperature steps Ramp rate can only be changed on the dual 48 and 96 well reaction modules The default ramp rate is the thermal cycler s maximum rate for the reaction module
7. I000000 000000 000000000 00000 00000 24 2 00000 00000 0000 III 000000 00000000 0000000000000 00 000000000000 00000010 00000000 101 31000 OOOO BL ELO D L S1000 uU 510000 0000 00000 00000000 LU DU DO DL B S1000 O DB DEI BEL EEL OLD B EOLD UO DLE 7E 7E CE BE DEBEO D BD 7B L
8. 2 00000 0 PERBD UBI U U CU C uua 0 10 00000 00000009 0000000 1000000000000 Tap 3j 120 0 00000000000 00 000 00000 55 000 0 000 00000 0000 20 0 III I I 000000 45 65 00 00L 0 2A410000 U 200 0 0000000 550 674d 0000000 PCRDI UD U DU DL U ELE BL EE BE 7 7 EO D UI L eoc pium 00 0000 ST 2 55 58 60 62 65 67 PCR I 00 O III III 24 Ul PCR 00000000000 000 0000000000 000000 d L 62 5100008 L D D U U U U LI C U D 4 i HH ET EE DEDE BE EET
9. L 060 00 000 00 0000 2 GOTO 00 0000000 00000 200000 utl 3 0 0000000000 330 240 000000 00 59 09 Ti mz 48000 000000 000000000000 00000000 000000000 Lut 53 51000 0000000000000 PAUSE TED DEL ELEL DEL DE D DL UL I HT L o BumgigpuuuiumuubDubbBbuPAUSED II JI B III 00 B BD 000000 1 PAUSE B D 00000000000 PAUSE 00 000 00 000000 U DO DO 0 D D D D D
10. OL DO D 11 51000 DB B UL OL O HELD DO DLE I D UE I stap Name Parameters and Ranges Description TEHP CRAT 70 TEMP 22 The target temperature between 0 0 and 100 0 C in tenths of a degree The hold time bebseen 1 zecond and 18 hours in the format of hours minutezzeconcds enter an infinite hold Forever pess the Infinite be y The loser temperature in the gradient Enter a number between 10 0 and 99 0 9C in tenths at a degres Uppar The upper temperature in the gradient The maximam temperature is 0PC Enter a temperature within 24 0 92 at the Lowar temperature The hold time bebseen 1 zecond and 18 hours in the format of hours minutezzeconds anter an infinite hold Forever pess the hey zem key The step number ofthe first step in the repeat ADDTHL REPEATE The number af additional times that the stepa repeat Ho parameters Atermperature p that instructs the thermal ccler to ramp to the specified target tempratur and hold that temperature for the specified amount oftime see step 5 en page 22 Atemperature step that instructs the thermal ccler to ramp to the
11. 6 Lid 100 28pL 8 FOR 3 F R n b Od F3 UB B D UU UO EI UI 2 0000 000 L 84 510000 0000000000000 L 510000 0000 0000 00 0000000 00000000 000 00000000000 III und 00000000 0 0000 0000 0000 051000 USB 000000 523200000000 5 MOLS JE 000 ga Wd B U EE 7E 000000000 USBII O OO 1 TOOLS 0000 DEFRULT GRRDCRLC PORT TooLS pono opp d b 00000000000 LAST RUNO D L1 HL OD DEB UD DI I UI L
12. 00 00000000000 000 0000000000 000000000 00000 0000 00004040 000 0000000 po Boggugu OO 000 0000 000 CREATE O 00000 000000 DELETE I 00000 000 RENAME 0000 000 L 79 51000 0000000000000 B DEL B ELE EF 000000 UU I III III III I J OFLES NEWDDTDDDD L 510000 0000 000000 000 0 20 0000000 00000 1 NEWD 0000000 000 O O II BLU ONEwO E ET BEBE UO D BLU E UE
13. S1000 0000000000 31000 I U 0000900 0000 09000000100 AO 51000 0000 00000000000 O O 00 051000 I I III 0000 000000 II 000 000 000000000000 1 51000 10 0000000 LU 15 2 3 ON
14. 0 0 0000 0000000000 VERSION HEADER LU 5 gt NAME rpg ul 00000000 1 88 31000 200000 I 510000 000 0 00000000 080 000000 0000 00 00 000000000600 Enter Cycler C1000 LH LH LU HH 0000 000 0000000000000 0940 0000 20000000 1000 0 0000 0000 000 100000 D 00000000 uua 0000000 0 510000 000 000000000000 000000000000 30 DD UU 0 0000 000000000000 0 0000000 00000000 Enter Cycler HEHDELBS DEFAULTS QO D EID 0000000 0000000 00 TOOLS gt DEFAULTS D 0000 000
15. CONTRAST DE 000 000 0 000000000 LITOOLS gt CONTRAST O DU DU 000 1 CONTRAST 0 D 00 00 00 CENTER TEE DD OU D DC DL CL D DD l RUH GEHDCHLC UERZXIUH HRHE PORT 20000000 0 0 92 51000 0 00000 00 00 00000 EHTER T ACCEPT CAHCEL T REJECT 3 00 00 00 00000000 0 D 000 0000000000 00 0 500000 000 000 DO 0 00000 OO O 00000 0 DUC OC 0000 00 DU 000 PORT 1E D BEI BLU D D LI LI L 0000000 00000 00000000 gt PORTOO COD DL DEL D COL 0000000000 000 080000 0 000000 1 PORT O O D UD DU 00 L DEFAULT GRHDCRHLC UERSIUOH HRHE FORT 93 5100008 0000000000000 DU U U
16. DNA 5 20 000 00000000 0000 00 00 0 000 000000 055 200bp0 00000000 0 00 0000 0 000000000 0000 00000 2 TEMP npppmb uuu 00000 10 0
17. 13 Function Descnption PROTCCOLB Copy an existing protocol fie ard ere it wih a new name For COPT E page 66 HONE Move an existing protocol file to another folder Fer instructions 57 DELETE Delete an existing protocol file For instructions ses 63 EEHAHE Rename an existing protocol fle For instructions pace 65 HEW Create a new folder For instructions 72 Protected files can be deleted and but onk after the RECUEE 3 2 pasword protecting themis used For inztructions page 71 DELETE Delete an empty folder For iretructians 73 folder cannot be deleted if rt contains protocol files EEHAHE Rename an existing folder For instructions ses 74 0 000 00000000 0000000 0 ppm 000 000 000000000 000000 72 51000 0000000000000 bELETED TEL BBL 00000000 000000 RENAME TL HL DD DU D ELE BIBLE 0 0000 00000 coPY O0 0000000000 0000000 O00000 OOOO II II OFLES gt COPYO DO O DD 10 00 000 00 0000000
18. PROTOCOLS M tE DELETE 51000 0 000000000 OMAIN ji 0000 12 00 000 000000000 000 2 D U U FOLDERS PROT0COL 5 COP Y SECURE DELETE DELETE REHRME REHRME Mau Folder ANHA 80 51000 0000000000000 DELETE TE 000 00000 000 gt D III D D III D HO D HEB DHL 000000 ubt 0000000000 0000 00 0 000 00 00 ud 900 00090000 0000 L
19. 00 00 040000 0 000000010 PROTOCOLS 00 000 0000 00 000000 00000000000 00000 000000000 0000000 000000 000000 00 0000000100 22 31000 FILESDCBDDBO O D O0 0 D OB HO DD BOO DU 0000 001000 ubt iub r3 L3 NeWwITDTDUU 00000000 BHO OU BEL EE III III O GEL I ETE TP D ED ELE ERE EE BE
20. 70 Id IHE ERE SEE EP 3 00000 0000000 000 III 00000 5 000 000 008 000000 I IILI IENTER JII 000 00 38 10 0 LU DO DEO EE BIO LT UI 2 au LIED 11 50 p AO 3840 00 3 e iii N E ENEN E EE E E EE E S 000 00 0000 00000000000 000000000 00000
21. 00000000 00 00000 0000000 beow 00000 00 0000 000 000000 0 31 Sarpe Voi O 00000000 00000 00000 89 31000 1DEFAULTS 2D D U UD 7 7L UE ED U UOCE DU DEFAULT SETTINGS Block A A Lid Target Turr off below m sample Mol DEFAULT SETTINGS 000 000000 UO III DD L Lid Targe T O O HH D D B g0 10561 0 I off 000 00000 3c O OOO utt 0000 01504 3840 00 00 00 0800000000000 3011 1 00 0 000000 0000 000 009000000 000 III I 000000000 3D LU U UL L No00 0 000 00 000 000 000000 D YES DU ENTER ET D E I LUE UE GRADCALC 1L 000 0000000000000 5 gt GRADCALC 00 00 0
22. ENTER 00 0000000000 19 1 1 Vol 196 45 0 FOR 0 04 45 07 FOR 8 28 55 8 FOR 8 3 GOTO 2 30 TIMES 19 4 FORENER n A ah 40 510000 0000000000000 4 5 0 IB LI ZU TTE 28 4 sz aded 87 8 28 FORENER 5 D HgEmmiEiLmidmuutututbi L uggpBulultdtutiutiuiutulitiuttutiupiiiutiipiiuEeNTERTIE 000000000 00000000 00000 4000 0000000 11470 al FOR 4 3 FUR 8 38 a t FOR HB 3H Ao DING xe e D BB BED DE DU 1 ONS O 00 000000 DD DO GLO D CL LOO D DU CI 1000 000 004100
23. T PORT 2057606 510000 0000 0000000 c1000 0 0 00000000 10 0 5100000000 00000 30 BU d B BLBTI Il 00000000 000000000000 000000000000 WO0 L 5100000000 0000000 0000 C1000 0000 0000000 00000000000 510000 0000 0000 00000 OC1000 i Dn DD D BUD U 000000000 5 0 00000000 10000 000 0 0000000 0 10000 0000000000 000 90000 000000000 100000 USB OOOO 00 10000000000 D OD 0000 0 000000 000 0000 100000000 5 O 51000 USB B O O OO OO O 51000 USB A 0 O0 DD 00 COOL D O78 D OBUD Dd B DO D OD L 000 510000 0000000000 C1000 00 0 HB ELE 00 0 CELO D HE D LOC DC DE EE D OD D DEO UO 0 10000 0000 1000 51000 USB BL O 00 00 C3000 DD 000000
24. 50 00 000000 0900 0000000 0 dll t 48 10 0 40h L 0000 000 000000 Run ITRQFST on R RUN UI EW on AD 000 0000000 5 Run ITROFST iample Uol 2 5 RUN VI EW VIEWD DH 000 OENTEROO DEL I 00000000 Ul Run ITRU FST 7 sample Vol 2 RUH LI 00 III ug 000 00 0 0000 0000 5 L Lid 100 I D REN
25. 000 UO D POMVNT I D 800 ug uu 050000000000 1 19576 Val 18 41 Bo Doug agp OOU 0 0000000 000000000000 20 00 0U I 00 LIE LLLI LI 24 51000 0000000000000 III L Lid 105 C 10ml II III II CANCEL JII III U L 0000000000 DE BD E UO D B UD L Heu STDZGRAN 3 00000 0000000000 Uu uui 000000000 00 00 00 00000 0000 000 0000000 000 00000 00000000 6 110 L 000 0000000 110 L Lid 0 5 Vol off below TT D O0 000 00 000000000000 000 000000 00000 0O D DD off D 0000 00000 000000000 TOOLS gt DEFAULTS O O HU E 19000000 4 25 510000 0000000000000
26. Running on R ITAGFST 24 200 ERE TF 5 Temp 55 PAUSE 1 Running on IBS DE 1 45 0 Temp 38 87 1 2 5 00000 1 6 5 Running 46 87 8 38 81 8 B 54 51000 0000000000000 10000 000 0 0000 000000 00000000 005100000 DO L 000000 000 1 000 000000000000 ENTERO ODL D HO 000 00010000 I ud 2 30 400 00 000 000 00 00000 Running ITR F 3
27. 000 000000000 0000000 00 00000 80000000 0000 0000 000 000000000000 00000 82 31000 III II U DE D t Blocks are idic 20000000 PROTOCOL MIU CE 30000000000 L LD 0000000 000 0 00 000000000 PROTOCOLS 83 510000 0000000000000
28. Ul RUN O NEW EDIT FILES VIEW TOOLS 21 5100008 0000000000000 ul 00000 0000000000 0000000000 OUUU OL iiid diligi dii idi 000000 0000000000 RINIITEDDBDBOUBBHBE BEU E C 000 0000000 00000 utl 0 00 190000000000 gil O MAIN DID EE EEL ELO DD HL DEL D IU L J
29. ucANCELT IT 180 0 A0 uU Dou 3D0 UU LU LU 1 11 13 Mol FOR 3 88 3 FOR 8 38 FOR d 36 31000 4 00000 RAMP O 0 000 00 0 0 ORATE RATE 0 000 00 000 0000 BD LB BEBE D RAMP E E C RAMP 1 0000 000 00000 00000 000 00 Uol 2B8pL FUR 4 H8 FOR 8 238 A t 5 2
30. 1 UH DUO UD DEL EI O INCUBATED D D LT DI 0 000 00000 00 000 00 00000 INCUBATED 0 0000 48 000 00 000000000 0000000000 00 IU L 2 00000 0000 0 000 0000 000 009000000000 OYES 0 0 000 000 DIO 00000 I D DU Ll Usg 114 Ho 56 51000 LI D D B U U U LI CI U D 000 000000 0 1000000000 9 lt 1580530 3 00 00000000000 ZP I OENTER II J 1000 00000 0000 mi b 00 O OOOO D DU UO HIELO CIE EF ELE C D D OH CANCEL BET 00000 0000 000 000000 9 quiyuuuuutulu Incubate atf 4 00 000000000 D HE BIB 0000000 0000000000 RUH LI EH HEH FIDELES EDIT T 0L Incubatin3 at LESS
31. 000000 UL 0000000000000 0 0 O 0 0 15 510000 0000000000000 BIBLE EE EE EE BE BIET LI 1 2 2 0000000000000
32. 5 51000 0000000000000 5 CANCEL 1 HL BLEU D 00000000 CANCEL HH 00000 00000000 0 000 LEYES 000 NeD UO DB 7B DOLO UL YESI III II No00 I III WU LD ENTERCTEID DL UO Out uYESmDmduu B B U D 7 6 00 0000000 0000000 85 Run ISCREENII I I 20 000 LAST RUND O 00 0 000 00 000 0000000 100 q 000000000000 80 0000 0000000000 0 20960 00000000000 LAST RUH INCUBATE LLD VOLUME TOTRL TIME ERRU RS VERSION 58 31000 51000 0000 0000000 CI CI D II Fast 000000000
33. OOOO INCUBATE II D D 0000 00000 SCREENT HL HL D UU BL ELE II IILI DU PAUSEL E uu ulllgBBil ul Lu OCANCEL O D BD B BBEDBBOUBDUULU UU DUINCUBATE EL HL D D BEL B D OD D DO D D DEF 0000000 0000 00000 1 000000 00 RUNO ELO BIB DO ELLO LU rBleck is 0000 0000000000 00 00 000000 0000 R LH UI EDIT TOOLS Block idle 48 100 0000 0000000000000 00 0000000000 0000 0 0 ida 00 III 000000000
34. E 11 11 99 10 0 00000 00 000000 DOODO liti tl uH L 31000 utut UL I OOOO utt 052090 10 0 B Tp piti 2D D U UD UU U U 300 U D l 0000000000000 0000000 0000000000 AQ D BO DB 7
35. 05900 00000 00000 L 198 3 2 53 ri LAST H TOOLS 010000 00 0000000000 080000000 10 SCREEN 0 00 000000000 000000 SCREENTTE ET ETELEIL E E DU D ELE 0000 5 0000 480 00 0000 0000000 00000000 00000 0 0 00000000 B 5 JII 0000 SCREEN 0 JI 0000 0000 00000000000 LRunning 0 000 0 00009000 0000000000000 5 2 0000 00000000000 20 00000000000 00000 200 OOOO 000000 950 mum Temp Repeat 52 510000 0000000000000 2D 000 L Hbi UD B Bam Igual 200000 00 00 0000000 coro 0 0000000 00 000 0
36. HYH m g SEE EP 11 31000 0 0 000 0 000 00009000000 00 0 000 00000 00000010000 L OBlocks are 000000000 00 000000000 069000000 0000000 Hela FILES AED arg idl 12 510000 0000000000000 510000 0 00000000000 S1000 000 0000 000 0 000 00008 00900000000 0 00 00000000 0 0000 00000000000 00000000 0 0000 00000000000 00000000 0 0000 0000 0000000 00000000 00000000000 00000000 IILI UI l
37. PROTOCOL CANCELLEDT PROTOCOL CRHHCELLED Sor 000 000000 000 000 000000000000 00 00 00000 00 102 31000 l 0000000 0 0009000 0000000 0 uuo ull LU m Lu n POWER OUTAGE DLIRING CYCLE X STEP Y RESTARTED AT 27 2 CONTINUE PRESS ENTER PLEASE CALL BIO RAD FOR SERVICE BLOCK OVERHEATED HEATSIHK CYERHEATED PLEASE CALL BIo RAD FOR SERVICE PLEASE RESTART CY CLER STEM CVERHEATED PLEASE CALL BE RAD FOR SERVICE PLEASE CALL BE RAD FOR SERVICE ALL BLOCK SENSORS FAILED LESSE BE RAD FOR SERVICE POWER SLIPPLY
38. uuuudgulluliuluulutuuuillitLLLiLL I iL Lil t 003 6404 0331 0 0 0 DD DE UD DU 31000 H 5 5 0 UD mI 10 31000 UD S1000 LE 0000 E ee N LEEN ULL
39. 1 D 00 00 00 0000 DBENTER D ED 000 0 0000 PROTOCOLS MOVE REHRME REHRHE 2D uuu UI l 000 0000 000 mNTERDE DU UU 000000000 UO D BO D D BL DU l 3L U LU YESDUD 0000000 No 0 0 ENTERII III DDBUD UU 00 00 0000 HO D 000 0009000 00000 0000 81 5100008 0000000000000 RENAME 00000 000000 JFILES 00 00 DD 0000 000000 1 Ul FOLDERS PROTOCOLS HEH SECURE H UE DELETE REHRME 20000000000 LU L UD UD l FOLDERS gt 3 L
40. 50 51000 0000000000000 III l OOOO 2000000 0000000000 310 DO D LU L Running on ITRQFST step 3 65 8 0 10 Temp 65 8 Repeat 2 of 31 coro O00000 000 00 000 00510 00000 00000000000 00000000 III III UI l IILI 4000000 Running on Step 4 Temp Repeat 8 0000 00000000 000 00000000 0 0 Protocol corpete DDDU UU B D 00 00 000 00 O OENTER 00O 00 000000000 0000 5 200 530 DEL 000 00000 PROTOCOL COMPLETE Total 5 51 51000 0000000000000 9 LAST RUND 0000 EEE EL DUL SCREENII D DU I 000 EAST RUNO OL B EE 000000 00000 SCREEN T D II U U C 48000 000000 000000000 5 0 00000000 LAST RUND O 00 E 0000000 LAST RUN O0 HL
41. 10000 000 0 0000000 00 00000 LL 94 5100008 0000000000000 1 1 000 0000 000000 5 USBBIDTDDULD U UU LU 2 C1000 0 LB D 0000 00 U L USB A DEL DD U C 000 0 1000 D EL D 7E DL LO ETE D C1000 0000 00000000000 3 C1000 0 5100000000 1000 20000000 30 DD 510000 C10001 00000000 MAINE BELO UL TE BED 0000 00 000 01000 100 0 0000 000 BD B HH 000000 00 0000 5 51000 52100000 0 000 5100000000000 00000 LU OE OL EL BI OOU S1000USB A 00 00 51000 L 1 5 958 8 USBU L 2 1 U L USB AD II III DO DET C 0OC10000 E ELE I 111 510
42. SID2IJIIIIII I III I III 2 0 9600 Un LAST LID VOLUME TOTAL TIME ERRORE VERSION 8 5 86 510000 0000000000000 TOOLS 55 5 5 0 0000 LSELFTEST HU UuuusELFTESTT O 00 00 00 HENTER Dj 00 00 000000 0060 2D U 00000 0000 OUD 0000000000000 00000000 ui Bi OOOD 00000 00000000000 SELF TEST IH PROGRESS gt EEE 87 5100008 0000000000000 VERSION 0 ET EID 0000000 00000000060 510000 L1 LH D 0000 0 000 000000 0000000000 gt VERSIONTIT LVERSION 2
43. uduiuliuliiiiiiililiii iui 0000000 1 SECURE 0 0 HENTERTED III 000 000000000 FOLDERS PROTOCOLS HEH MU Y SECURE MOLE DELETE RENAME FEHRHE 20000000000000 U L DU D IUUD UD 00 00000 lt GRAHT gt jJ 0 000 100 000000 00 000000 000000000 3U DW ub uu UD 78 5100008 0000000000000 222000000 10040 DDEUUX HOD B III III UE l 0 000000 000000000000 00 00 0000 13 00 0 000 00000 Up uuum utu utt 510000 000 0 0000 00 00 000 000 0000000 0090000 0000000000000
44. 000000 00000000 DU Uu 0800 000 0 0000000 DUD L 13 510000 0000000000000 0000 0000000 00 000000 000 900000000 000 00 000010 510000 C1000 000 0000000 0000 5 00 061000 000 0000 000 USBII 00000000 510000000 O00000 000905800 00000000000 00 0000 lJ I 14 31000 1 7 10000000 0000000000000 00000 LU LL uuuibiuliutu il ul 2x48wel 10 50 uri mad 3 OOOOOOCOOODO III
45. 16 510000 0000000000000 UD dg pid Bp Bu 00000000 1 I L 2 3 d gg d ad X UO 70 OCECE EE BCED 7T BEC LD D L 17 510000 0000000000000 0000000 000000 00000000 0 0000 009 0000 00 09000950 0900 00000000000 U ut uU 0 O L DD IBI U DU UO ELE UE C LT D 000 00000 O 0000000 000 0000000 umi ipli ulubiiuitbulii LL BELDIEIEE BT 00 BL 1 D00000 Wb uu UUOggugdul lii O O OOOO iu 000 00000000000 2 0000
46. P iii BO UB 100 5100008 0000000000000 51 0 009 000 90000000 uU L3 000 0000000000 0009 000000 0 09009 00000000
47. 100000000 0000 S II 0000000000000 0000000000 GRADO ELE LEE DU B BED LI 00000 06000 00000 000 HOO D D O D 060000000000 00 00000000 Ud HU D 00000000 000 0000000000000 029 LU DO Ub 23 51000 0000000000000 L RUH UI EH HEH FILES EBII TOOLS Blocks are 141 510000 0000 000000000 00000000 4000 20000000000 Lid 65 gt Uol 2 0000000000 0 00000 000 0 0000000000009 000 000000 D 0050 0000 00 0000000000000 0 000 00000 0 00000 4080000 L
48. 3 l Il 4 FOR 4 BE sper EUe SPARA xe 58 2 FUR EXT RATE BEEP ENTER DTE ELO DC UU U 1 1 171870 Ua 4 4 SH FOR B 58 FOR B 58 2 aH TIMES A RAR ha 45 510000 0000000000000 000 0000000 00 0 0 d 0000 0000000000000 III 00000 ENTERO l 11 000000000 TFUR CC LE F R 38 2 38 FOR 5 88 Ho ENDIUDDBBUL UUUDO I IU LI END TELDIDO D HU U D ENTERETE III 7 7L 7I UU U L END TTTTIDE DO DOLI C 000 HENTERDTE 000000 B D B EO L DD UD DOENDO TELE BU BEL U L 46 Lid 118 L 4 FOR 510000 0000000000000 51000 0 0 0 000 0000000000 00000000 0000 0000000 000 000000000 RUN np p BOB IILI EI 00000000 PCRO DEO 000 00000
49. 0000 III 00 000 000000 PER 00 DUUUULDLUTOU UD UO CE OL COLO UCEU BE ET CIE Gene Expression Gateway bio rad com genomics HOO O OOOO 000 O 0 Application Techniques gt Quantification gt Fast PCR II E E D E 00 00000 Fast PCR ELEIBL II I D LI Fast 00000 0 0 3 O 0 00009 9000000090 00000 00 0000000000910 DO O L Ramp 000000000000 DD LE 7T DEBE EI BE EE C DO 7 UC CBE BT DU RCeRE D UD 7 7 000000 Auto Protocol Whiter 510000 000000 00 0 00000000 0000000000 Auto Protocol 0 O 000 00 00 000 510000 000000000 Awo Protocol 00000 0000000 59 5100008 L D D U U U U LI DIU D l 510000 0000 000000000000 PCRDDDDUULU l LU 02
50. Li d 118 Us 28 BH F R 4 58 45 8 FOR 8 38 2 IHC ExT 9 BEEF 3 IPAUSELI II DO DU 20 gau LI d 118 al 4 B B 3H SECOHD 2 37 31000 4 300000 RAMP ONO UD 00000000000 rn D 0000000000 0000000000 000000000 000 0000 000 O 00000 00000000000000000 00000 00000000 0000000000 000000000010 O FOREVER BEEP 0 0 00000 0000 1 0000 000 00000 DENTERDU 70 00 00 H zu Lid i118 C a 5 3 al 2 18 8 FOREUER 2
51. 00000000 0009000000 00000 0000 0000000000 PCR DU U HODET 20 00000000000 0000000 000000000000 0000 0000000 090000 00000 0000109000 60 51000 L1 D D U U U U LI CI U D 000 0 0 UD rTEMPHDDEU III C727 D D EEELEEL E E DEO DE 0000000 0000000 t uw 0000 0398 1 1 3 2
52. TUBES AMD MICROPLATES CAPS AMD SEALERS Description Catalog Prefix Tubes Caps TS Description Volume ancd Humber Catalog Prefix tubes with caps 0 2 TFI and strip tubes 51 0 2 ml and 12 tube Strip tubes low profile 0 2 ml amp tube 5 4 Micropxates Film M5 Caps TC S 66 31000 TUBES AND MICROPLATES CAPS AND SEALERS Unskirbed and full height 24 48 and 95 A E F Sirip Unskirbed and low profile 48 and 95 A B F P Skirbed and full height 90 and 384 sel B F Strip ISP Skirted full height and and 384 sel B F Strip hard shell BSP he prefix the catalog num or each series af supplies 67 31000 E Un u s BOB OB BE EE D 5
53. 5 00 0000000 0000000 VERSIONI I 0O LO D B 0000 00000000000 NAME T 0000 000000000 DEFAULTSITI 0000000 00000 0 OLtum off belew TI O 00 00000 GRADCALC T 0000 000000000 CONTRASTO 00 00000 00 PORT B1000 0 HD LL 00000000000 0000 L LJ PL LJ D 85 51000 0000000000000 LAST RUNT 5 LAST RUNDE 0O DD DU 00 000 LLAST TOOLS O HL JLI HEAST RUN B EL HL III HD HD DELEE BE III 1 L IENTERIIII HB BL 0 000000000000 TOOLS LAST RUH DEFAULT SELFTEST GRRDCRLC UERSIOH HRHE PORT ALAST RUNI 1 EH J LAST RUN 1 HE 000000 00 00 000 000 00000000000 D D ENTER III III 0 000 0 000 OLAST RUNI uui 00 uL LI LE LT 00000 Turn off 0 Lu Uu ut tL tL 510000 0000 00000 000000000000 L tr
54. 1 EJ EJ p3 P3 EJ aoda log Ia EJ EJ E3 CJ EJ EE G Da dq 15 i m ca L1 O O n r4 L4 L3 r3 r3 T N M 5 ES I gooo Jo P 51000 510000 0000 000000000000000 00 000 0000 UD Bp Bi L L 3 805 200 J 0000000 e 61010 12001 20 120001 O U 61010 2 010 2005 JEN61010 2 010 2003 20 0010 00000000000 00000000 61010 2 081 pO014A1 JEN61010 2 081 20024AYI E E DE 000000000000 2 605 L L 11000 e 61326 pooo D 1 I D D
55. 200000 Lid 188 C al ZEAL 4 F R 8 38 0 38 10 99900000000 000 0000 L 31 LU DULL LI 30 l 31000000 30 31000 Lid 1887C 4 58 2 HL REPERTS 30 00 0900 000000000000 Yee I 000 00000 000 000000 Non I Hl coror iiu 000 000 0 0 00 00 000 000000000 at FOR 4 1 38 5 ATS 0 38 2 36 31 31000 9 000000 000000 00 000000 dili iti 0000000 00000 09000000 ENTERDD 000000000000 100 000000000000 980 TEMPDeRAb D OU eoronpmnmp m I B BUT D BL Dg guutuiuiiuiubimieMPpnmm GRADO 0 00 0 00000 ENDETLI ILU I uut BEEP 000000000 D DU 00 0
56. 00 00 000000 LU 000000 0090000000000 LL 97 10 0 BE O 010001000 LTD LIELEEL EE BL DL DEBE CLE BED DT L LU UL
57. EAT ED PLEASE BE RAD FOR SERVICE HEATED LID FAILED PROTOCOL CANCELLED PLEASE CHECK AIRFLOW HTSIMK OVERHEATING PLEASE CALL BIO RAD FOR SERVICE PLEASE AIRFLCA STEM CVERH EAT ING PLEASE CALL Blo RAD FOR SERVICE FL u HEF SIR PS OVERHEATING Cau Eis plane d shen a machine running protocol has been turned off either intentionally or due to a power outage and then turned on again Heaction mexdule has excee cec maximum temperature of 107 5 or zensor has a malfunction andis nol meceurinc temperature accurately Protocol terminated Heatsin k temperature has excee cec TFG Protocol te mnina d Amplifier 1 temperature has exceeded amp 5 7 Protocol temninate d All black sensors hae belvs Tor falu contaria Protocol terminated Power Supply temperature has exceeded 5 Protocol terminated sensorhas failed during kd pe Protocol terminate cd Heatsink has exceeded Sytem be eps and displags error Amp tmp hase System Le eps and displags error Power Supply temperature has UPC System and PLEASE CALL Blo RAD FOR SERVIZE displays error 103 zeige C HIC HAb FUE sLOW BLOCK CYCLING C HIO EAb FEH SERVIE sLO W CYCLING PLEASE CALL BIO RAD FOR SERVICE SLOW GRADIENT PLEASE CALL BIO RAD FOR SERVICE HEATED FAILED PL
58. 00000 00000 65 51000 LI D D U U U U LI C U D LU 1000 00 000000 000000 00 00 0000 a 51000 00000 0000000000 000 00000 510008 000 0000 000 0000000000000 000000 00 00000 1000 0000000000000 0 08000 0000 000 00 000 00 00 000 00 00 000 00000009000 100 00 0000000000000 0000 00 00000000000 0000000 PCR I TU 10
59. or aiuiu tu 5 3 14 110 Mol 4 FOR 4 06 5 3 FOR 8 38 4 uuu uBDpuubpuuuvesutut LU 35 31000 UD Bulb IU L 1 TEMP IDODD 00 000000 OPTIONTT ENTER JI CU UE 000000000 000 00 Hew Lid 11B8 C STD 2B8pL 35 6 FOR 4 88 8 3H 00000000 0 00000000000 00000 0 LU 2 000 U ll 3 DDDBIIULUL ul
60. 000000000 0000 00 00000000 000000 000 0000000900000 0000 000 00000 000000000000 100000000 00 00010 00 000010 00000 0000000 500 D UO UU off below TT D ED D DO 0000000000000 0 000 000000 0 0000 DTum eff below 000000000 000000000 TOOLS gt DEFAULTS Q0 0 000 0900000 39 51000 0000000000000 EDIT 1 HL ELELE D D D DELE IU l EDITED O DU DL DO 0 B CT ET D DD L EDITITD O DEENTERTIL EL I IC LE LU RUH LI EH HEH FILES EDIT TOOLS Blocks arg idlig UU 1 0000 000000000000 0 II I I 00000 0 III III L 24 it PRO TOCOLS ITR FR ST STD 3
61. 5 6 6 Expression Gateway bio rad cor genomcs TI O Automation 0 000 000 00 00 000 0000 0 000 00000000 uuulutlulti iultutLiiuiuiuututttLL ttt 10000 00 0 ucior p 96 31000 i iL O O OO 051000 00 0 0000 0 00800000000 0000 III 00000 00000 0 pj 510000 0000 00000 000 000000000000 51000 1D HD D HUDD B BB HH 00000000000000 D D D 00000000 uU iil 0 510000 0000 0000000 000000 510000 00000000000 00000000 00000 0000000000 000000
62. ais 01000 0 00000 00 00000 00 000 000 0000000 OL IILI 0 00000 0000000 00000 gt 00000 000000 TEMP T LU LC L TEMP eRADDDDUL UU U UO C D D U D 0230000 enuutuiuliiuENTERITE 000 D U U D EL U D D L Press EHTER 0000000 500000 001 100 0 Q 0000 0 000 0000 000 DD TEMP D LED UL GI C O C DO L 2 TEMP 9001 1870000 180 0 CT LU U D L 0 000 040 LA OO I 26 31000 40 000000 DO O O OYESONOL
63. H 10 M RUE O LT LT 8 RE RR E RT RR 8 9 10 EE TET 11 S 0001 3 B bi 11 a T Ba 13 rtt 15 E 16 RE 18 u nu on 20 Y 23 NU VERE Dn 23 D usse 34 E uu 34 36 37 38 39 mia s 8 39 40 RE 4 47 31000 SCRE 52 PAU BBB TS 54 55 uuu uu 55 56 5100008 0 00 000
64. T zmp 4 of 256 00000000000 CANCEL 10 mm 0000000000000 U 000 0000 0100 1 CANCEL O 0 0 YESTI CANCEL 00 000 000000 Non I auput III ENTER DE 000 LU DB HE UL OL D Running on 2 Temp Repeat Cancel run 480 00 0000 000000000 000000000 0 CANCEL 0000000 55 s10000 0000000000000 2 D PROTOCOL CANCELLED 1 1 D H D CANCELLED 000 uUidd uiu idWmil idiiitui litLil tll 570 0 0000000000 00 0000 PROTOCOL CRHHCELLED Total hime EE SENTER 0D 00 000000000 0000 INCUBATET 00000 00000000000 0 DINCUBATRHRATI III UU D 0000 000 000 000000001000 DCANCELTD O JII 0000
65. gt amp EVA gt lt GRRHT gt DELETE 00 0000000000 5 gt DELETE I L BBOd igi iB O OOOO ID E 75 510000 0000000000000 1 FOLDERS PROTOCOL COPY SECURE BELETE REHRHME RENAME 2D 0000000 900 00 9000000000000 00000 90000 000 000 000 00 PROTOCOLS 3D D L 500 0 OU U YES DLE 0 DD ENTERD D D 0000000000 00 10000 000000 00 000 090900000 000000000 piu L Ub uuu 76 51000 LI D D B U U U LI CI U D LU 0 000 00 000000000 0000 0000 0000 00 00000000 5 gt RENAME O 00 009 0000000000000 0000000000 BER
66. E I DU 73 5 1 0 00 0 0000 000 0000 00 0000 00 00 00 0 000 Noli I JII Yes 0 00 00 UDDDDYESDDUDDDUDULULULU Edit 194 110 Mal 2B8pL 45 8 FOR 4 145 8 FOR B 3B 025 B FOR 8 58 8 GOTO 2 38 TIMES changes Pe eJ L L j T3 43 510000 0000000000000 3 EDIT OO I DE B L EDIT II HL BIB EIL D D DOE 00 000 0 3 569 3 5 B E At FOR 8 3 5010 2 58 TIMES 000000 3 00000000 5 40 6000000000 STD3 Uol 2 1 95 8 4 88 35 8 FOR 58 F R FUR GOTO 2 28 P DUI 20 000 000 000900000000 000 009
67. 1000 00 000000 0000000 000000 000000000000 0000000 BmiuDuttlu 100000000 0000 0000000 0 2 1 184 0096 10008 00 0 0 0000 0000000 96wellr o 2r 184 0384 10008 00 0 0 0000 0000000 384well 31000 Gene Expression Gateway www bio rad corry genorrics PCR OD EE B D 7B EE 7B EE U D Life Science Research web site bio rad corri DIBBUEBUULbdguUttuutiiutiutuitituiiui it uuu liii lJ iuubiuuui iilii wduututuilil L U 4 6800 000 0000000 00 0000 00 0000000000 L E uuu LL 00 radcorri T Gene Expression Gateway rad com genomics O 00 0 D D 00 uuguuiulutttttlulutlultl 10000
68. 30 5000000000 0000 76 100 Q C 0000 180000180 0 lI 0910 0 00 TOOLS GRADCALC UD D D UI 0 3 000 Ul 50 000 00000 000000 00000000000 000000000 3 EE TT EN 3 Ho ption 28 510000 0000000000000 H zu Lid 188 sTDSGRHD Mal 1 4 F EE ipta B 3d Lid i886 Uol 28 amp RL 8 F R 4 88 4585 3775 0 8 38 4 OCANcCEL0 O 0000 0550 2 FROHT ROH
69. PCR D EU III III D D U 30000000000 000 300000 DNAD O OOO O DNAD 00000 000 000 D NAT 000000 200000 000000000 66 0 PCRO III L 0 000 0 000 0 0 000 00 51000 00000 000000000000 0000000000 0 000000000 05100 10000 000 1 l 400 00000 0 0000000 0000000 LTEMPETI 000000 0 0000 L cororeoramim m OPCROO L O O CENDO DL ID DD D DHL D HEO 7 7E UL EL D D 7E 7E C EE UL L O O 0510000 0000 00000 400 00000 00000000 0000000 4 ac gt 69 31000
70. Toggles between screens for this key to view the status of alternate views a run AND SYMBOL KEYS 1 through 9 Press each key to select numbers PAUSE Enter a decimal point or pause a protocol INCUBATE 0 x Enter a zero begin an instant incubation or enter an infinite hold Enter a minus sign or cancel a Press this key to delete an entry function cancel a function stop a protocol end an incubation or delete text on the screen CANCEL NAVIGATION KEYS RIGHT ARROW Move cursor to the right LEFT ARROW Move cursor to the left UP ARROW Move cursor up Press the up arrow key to scroll from 2 to z on the screen For example press the up key three times to select the letter DOWN ARROW Move cursor down Press the down arrow key to scroll from z to 2 on the screen For example press the down arrow key eight times to select the letter 5 ENTER Confirm a selection a command an entry or to open a folder 20 31000 HEHDEL RUH UI EH FILES TOOLS JETER arg 4 lJ 5
71. 100010 Ul D ud 00000 00000000000 UULU DU UU D DU E U 450 000 0 PCR ODO B BD BE BU DU L 0000 00 000000000000 61 51000 0000000000000 GRADO 1 EIE E D D HE TEMP TEL ETE UL GRAD 1 j ing i Bad BB BBB DNAN DOUDOU EE Tm ED DO HL OD EE CD IU U mim
72. see Interchangeable Reaction Modules on page 9 Instructs thermal No range Only applies to cycler to emit a temperature steps sound when it reaches the target temperature in a temperature step 00000000 000 0000 INC DO 000000000000 0000000 0000000 0000000000000 1 00 0000 34 31000 000 00 0000000000000 110 Hol 28pL 3 68 8 5 2 A t F R 4 FOR 8 238 A t 3 ExT RATE BEEP 3 00000000 OCANCEL III D UL 0000 ENTEROO 000000000000 1001 1600
73. 000 000000000000 0 00000 100 000000 00000 00000 3 00000 18 510000 0000000000000 Di uuu LLL 00000000 lbi OOOD 1 2 00000 000000 000 U U 000000 BO DE BE D DEOE B BEBE C 7D 7 CIE 000000 0 0000 00000 00 000000 00000 000 0000000000 Bub 000000 idt van 00000 00 00 00000 008000000000 000 0 0000000000 V4 D DU UCI UD C 02 0000000 0000000 D 19 51 5 lJ lJ ooo 7 000 4 ooo 000 S1000 Thermal Cycler 0 8 0000000000000
74. Option 3 Ul 5 000 00000 000000 00000000000 000000000 DC DEO B B I D L EXT RATEO 00 II U 1340 FOR 4 08 Ho pfion 6 eRADDL 0000000 UD DD 5 0 Press 27 31000 20 4 FOR 4 88 638 992 LU LLL 24 Lower 50C O D UU U U L Upper 74 Qd 900 0000000 000000 GRAD 0 0O00 UU DEO CL U CLE 308 0O OOL DUppeert TTE O O 100 C
75. X STEP Y PLEASE RESTART CYCLER PLEASE CALL BIG RAD n E 31000 iduals onig Lid has overheated anc has been shut off and protocol has been terminated Armmplifier temperature sensor 1 has aile d Power Supply sensor has Tale d Poserta blockis out of range Logic power sgnsor is out of bounce Hase power sensor is out of bounce Protocol cancelled Amplifier ternperature sensor 2 has aile d modue has been remover Protocol terminated Protocol storage memory has been corrupte d to read information from reaction module properly Information read trom reaction appears incorrect There was aproblem with the block and power w2 shut aff ofthe block sensors in asinge block has faded The system has cancelled the protocol at step x cycle zent the block to 4 C to preserve Ihe samples Bl 00 p 105 00000000000 0 140 0002 III DU D D D D 22 24 DB Dump 000 20 Tel 03 6361 7000 Fax 03 5463 8480 5 gt 11411 Tel 06 6308 6568 Fax 06 6308 3064 0812 0013 2528 Tel 092 475 4856 Fax 092 474 5580 Tel 03 6404 0331 Fax 03 6404 0334 M
76. ate 87 totum eto deae 88 NAME tense ioc R 88 DEFAUE iS IIB ao uu Susa 89 u u cents 90 CONTRASTHDD ELE E ELE DELE NI 2 a y 92 PORT ETE Re uuu 93 10000 5000 0000000 510000 0000 BBBB DD D usse 94 ST000 E ET a otto eter eed ote etd 94 51000 5 C10000 0000000000000000 95 10000 58000 000000000 510000 0000 000000 nnn 96 733337232322 96 97 S TOO ET EL i piq ui ER EET ET D E 97 2 aa asan a nas sunnah 97 NBI a 98 100 101 iu 102 UE EE DM MM LE 102 ERREUR 102 31000 5100 00000000 PCRE D U DO C D UO OU ELOE L 00000 0510000 0000 000000000 00000000000 0000 O0 10000 00000000000 L 1000 HE E E B EL D E 00000 000000
77. lid sensor has failed H a dual protocol terminated f asinge ard BIO TH lid sensors failed protocol terminated and block to 47 Left heatsink sensor has fail eysbem using average of amplifier lemperaturesto continue Hight heatsin k reor has farle system using average of amplifier lemperaturesto continue 0000 000000000 00000 000000000 0000 000000 20000 0000000 2 0 0 000000000000000 ug 0000000000000 00 00000000 000000 9100 BU 000 104 Massage 1 CI OYERHEATED AMD WAS SHUT OFF SERRE ICI Hlo RAD FOR SERVIC TEMP SENSOR FAILED PLEASE CALL FOR SERVICE POWER SUPPLY SENSOR FAILED PLEASE CALL BIG RAD FOR SERVICE BLOCK POWER FAILURE PROTOCOL CANCELLED PLEASE CALL BIO RAD FOR SERVICE POWER FAILURE TEE CALL ETO FAE BASE POWER FAILURE PROTOCOL CANCELLED PLEASE CALL Blo RAD FOR SERVICE TEMP SENSOR FAILED BLOCK MISSING PROTOCOL CANCELLED PLEASE CALL BIO RAD FOR SERVICE PLEASE CE HIG HAL CORRUPT PROTOCOLS MAY BE LEST FLESSE KES i BAD REACTION MODUL E PLEASE CALL Blo RAD SERVICE PLEASE RESTART CYCLER INCORRECT CHECKSUM PLEASE CALL BIG RAD FOR SERVICE PLEASE RESTART CY CLER BLOCK POWER SHUT FF PLEASE CALL BIG RAD FOR SERVICE PROTOCOL CANCELLED SAMPLES CCOLED AT 4C BLOCK SENSOR FAILED AT CYCLE
78. specified taget be ripee rature cqradient across the block and hold thattemperature gradient for the specified amount oftime see step on page 231 protocol step that instructs the thermal coyor to repeat a set ol stepa torte specified number of Imes see Step B on page 25 HO TE The tota number of cycles in the protocol is the number of Soro repeats plus the first ogole protocol step that instructs the thermal cycler to finish the protocol Step 10 on page 271 510000 0000000000000 510000 0000 0000 0 GRADO 00000 bo mum II I IL 400 0000000000000 M RATE BEEF 200000000 D Hd dB D DEXT O E U DE UOCE ET C UE E D PREUT l 1200 0TEMP 0O00 BOE D BEL LI 12 00000 Option Parameter and Range Description INC temperature from 10 0 Apples only to a temperature step incrementi ta 10 092 per cycle in perge 25 Instructs the thermal cycler tenth of a degree to increment change the target lerperature af a step with each cycle where a postre number increases the
79. 0 0 00000 0000 III IILI Doctor O 00000 U DIO 00 Doctori 0000 Gene Expression Gateway LJ 000 000 genomics O JSupport gt Centra gt Doctor 000 CIE III III l uu uuu 000 00000000000000000 E 64 10 0 LL gau PER EL ELO DE L PCR ID D BLU D HU HELL EI D DE DB DE DE DEO OE III EI LD 0000000000000 00 0 000000 0000 0000 00000000 000000
80. 0 00 5 00000000 HII D D B EB BU O ORow ADO EU U CELO 7E L L Ut 90 510000 0000000000000 1 GRADCALC OGRADCALC D HD 00 00 ENTER TIE III D D DB B 7 U UU l DEFAULT GERHDCRLC PORT LI 0 00 000 00000000 0390 9910 000 00 0000000000 0 0 00 00000 3 ICANCEL 2 00 0000000000 SA0 00000 l LOHER TEMP 55 0 A D D OL D DE DU 000000000 000000 O BU 0000 00000000010 91 510000 0000000000000 000 00000 74810060 00000 8 FRONT ROH CJ cr r m irp 2 D T 5 5 BACKE ROH
81. 00 7000 ODEL I HB 00000 00000000 000 DOCS III 8000 000 CES DE D DE 9000 000 00850000 100 0 0 INSOO OOO n 0 0000 TEMPOOOOODO I BED DE L U Save Changes TI I HD D 0000 LED 00000000 D 00000 0 OYES DOLI D 41 31000 INS O0 D D I 0000000000000000 0000000 000 000 IU D UD D U U al 43 FF 4 1 38 F R TIE 4 FOREVER EDIT II DO DD D D B D U U UU DICE L Wol zZBpL 5 55 H B 38 FORENER EHTER fo edit D BUD 0000 0000 EFTE p L a ZBL F R
82. 000 5 Hew 00000000 0000 000000000 EDT 0000000 0000 00000000 LU DD DB ELI MOVE H 000 000000000 gt tu 0000 0000 00 00 00000000000 0 0000 000000000 000 000 00000000 74 31000 1 FOLDERS PROTOCOLS NEW SECURE DELETE REHAME 20 0 000 0000000 3 D 000500000 00000 0 IU L Jos 2 fo lt
83. 000 47 31000 2 ENTER 3 0 000000000 4900 00 0000000000000 5 BLOCK A j j BLOCK 8 O OOOO l HOD OL UO lI
84. 0000 000100000 EEE EE Lii 840 00 0 00 000000000000 3840 00 00000 0000000000 00000000 000000000 00 00 0000 63 10 0 510000 000 0 0000 0 000000 00000000 I I 0000 0 100 0000000000000 0 00000000 1000000 00 BU DUC U 0 10000 00000000 0000000 L 0000 0 00000000 00000 00000000 00000 0 lt 10000000 0000 LU DULL 0 000 0000000 000000000 0 DITE ODUO LU D EE OD B BODIE BILE D ELO 7 DT CI DELE D 0000000 1000 000 0000
85. 00000 uuu 59 Fas NO RETE 5 62 63 u 64 64 uduutulituliuitulbuliuluiuiuiuLiiilbLuttLlL eeeeeeee 66 68 Red 68 OS 71 72 72 COP Y u u u u 73 MOVE 74 DELE DETIENE uum 75 a n 77 SECURE EE 78 79 NP E ia PE P DD 80 eet deter pentes Rte 81 RENAME n teretes 82 uses 83 85 un EU DE CEU 85 86 31000 enserre autetn
86. 000000 000 0000 3 20 00 5100000 0 0 51000000000000 OOOO 5 000 00000 000000000 usBBIITUDUUE 510000000000 510000000 0OC1000 0 000 0 0000000 II USBD DH 000000000 10000 0 510000 00000000 OOOO 1000 20000000 30 DD 510000 C10000 II III LO D DO DU U 95 10 0 gt C1000 H BH BH BD DH ODDO 1000 000 BL LE III 00000 0000000000 910000 HD D D DD U U 910000 UU U U 10 000000 00 051000 U 5100000000 000000 510000 000000000 C3000 C CLE DL LO HEC D 0 BEI BD BEL D LU D LE 0 0000000 00000000 000000000 00 000000 0000 001000 SCREENDI ITID 00 B BB HDE LU BOE EE EE EE E D OB BOT UE EE D EL DU PAUSE 0 51000 I L LL E HH 00000000 0000000000 000000000 UL j ENTER HL ED H HD HEEL EE O 0 510000 000 0 00000000 000000000000 00000000 00000 0000000 l RS2320 000 00 100 00000 00 IPORT
87. 000000000 0000 ggg 0000 DO DD III III L 50000 00 00000 dif TD3 Mal zBrL q5 B 4 q5 8B8 4 Zi 1 aj 09 s 1D3 Mal q45 8 4 BE dure 5 8 Jale SIUE E TS qaum i D 44 31000 3 Edif Lid 118 C sTD3 Ma s lt 1 5 38 ES dn rg Press EHTER edit 10 000000 0000000 0000000006000 0 I 00000000 008000 000 0000 0 000000000000 Lid 118 L Mal 4 4 jugo 95 1452 ANLE e DEL ij 0 000 0000 ENTERTTE OOOO
88. 2 38 TIMES FU REUER Am a TEMP 72 000000 000 000 00000 0000000 03400 00 INCIJEXIIIRATE I 0 HOD UU U 00000 00000 L POS 118 G Ua zBpL 3 2 38 TIMES F REUER EXC P T I 42 31000 OO 0000 000 000 0000000000 12 Ua 9 B 2 36 FOREMER Ho pfion 8 0 000 00000000 0900000 III 000 DO 0000 0000 ti LI 3 Mal 45 8 F R 4 F R SE TS cie LION EDIT 000000000 000 UJU UU 00000000 000 0000000000000 0 0 00000 40 0000000 00000000 500 000 00 0000 4000000 6 000 00 0000 50000000 000000 191738 D S a 2 5 8 4 5 8 55 B8 8 FR B TIE f FA CI LE O1 E2 e
89. 3 E ES o o 3 ooog Ec 5 L3 3 r3 F3 Es 3 E o L3 r3 C3 r3 H L3 L3 02 L3 O o po 0 E Ex wa p Ed E 05 5 L3 ri L r3 ES goong L2 r3 L p3 c3 r3 HO 3 C3 3 5 r3 d L3 o0 70080A L3 r3 p L3 n L3 L3 p Em E EHI 3 L3 r3 L3 r3 3 a 3 oog E 3 ELE O r3 oad pH 3 3 r4 C3 E3 g C3 L3 5 02 05 3 p D prr o go go C3 n 3 r3 o o o L1 3 r4 5 mig 3 5 02 o2 cumul EE m 2085520 mH E cpm ma 280200 pt g H H L1 L3 5 p cru HE E 2 L1 a a a H o 5 L r3 L3 O r3 L3 L r3 Cm 3 E E ES 3 r3 C3 L3 r3 L3 r3 3 0 3 r3 L3 o Og 3 r3 L3 gt Cr mn rc ir r1 FL Ir O L3 P3 1 1 aA 1 ES L1 3 rnm Ex ES 202 5 n 02 a L3 C3 O05 m Fi 3 C3 p4 p4 oono g
90. 380000 10 END J JII OU DUBBI III 000 00 END UL 000000000 000000000 ENDELE O 0000 ENTER TEE ET OOO L LU 51 TIMES 2 8 F R GFRD G T HD 55 ENTER 00000000000000000 0 0 000 Nep B B BEL U UEELU DOLO U ET 2 38 TIMES F R Ho 32 31000 1 000 0000000000 00000000000 0000000 MAIN 000 000 UD iB p i L O OUOOOOCOCO OO OOOD L OOOO DGRANT 0 000 B BOLD 0000 L EUR gt lt GRHHT gt
91. BE DD L 1 0 000 HENTER TE 000 000000000 FOLDERS PROTOCOLS COP Y SECURE MOLE DELETE REHRHE REHRI1E 20000000000 U U L t L Ud guild 00000 0 000000000 OENTERO L OOO OOO QOUOOOOCO 0000 0 0000000 SID22II 000 00000 PROTOCOLS GRANT gt 77 51000 0000000000000 SECURE 0 0000000 000 0000060 0 000 0000 0000000000 gt SECURE III 000000000000 0000 009000 000 0000000000000
92. EASE CALL BIO RAD FOR SERVICE BLOCK SENSOR 0 FAILED PLEASE CALL BIo RAD FOR SERVIC ELECE SENSOR 1 FAILED PLEASE CALL BIO RAD FOR SERVICE BLC amp K SENSOR 2 FAILED PLEASECALL Blo RADFOR SERVICE BLOCK SENSOR 3 FAILED CERE CALL BIC RAD FOR SER BLOCK SENSOR 4 FAILED L rB li E iE BLOCK SENSOR 5 FAILED PLEASE CALL BIO RAD FOR SERVICE LEFT SENSOR FAILED i PLEASE CALL BIO RAD FOR SERVICE RIGHT SEHSOR FAILED PLEASE CALL BIO RAD FOR SERVICE LEFT HEATSIMK SENSOR FAILED PLEASE FOR SERYE HIGHT SENSOR FAILED 510000 0000000000000 Cause Hack taled to achieve target in the eshma d time id taled to achiee target in the eshma d time Block faled to achieve gradient in the estimate d time ingles Ithe right and left lid heater channels desiate om each other bg more than 5 the lid 15 shut att Block sensor 0 has and the protac o was Hack sensor 1 has and the protocol was teminated Block sensor 2 has and the protocol was Block sensor 3 has and the protocol was teminated Hack sensor 4 has and the protocol was Hack sensor S and the protocol was teminate d Left lid n or has 5 f dual protocol terminated f asinge ard BOTH lid sensors failed protocol terminated and block snt to 47 Fight
93. SLIBEIBE 0000 0000 O DOOL Bio Rad Laboratories Inc 2000 Alfred Nobel Drive Hercules CA 94547 800 424 6723 10010424 uiid This base unit Serial No in combination with its immediately attached Bio Rad sample block module s constitutes a therrrel cycler whose purchase conveys a limited non transferable immunity from suit for the purchaser s own intemal research and development and for use in applied fields other than Hunen In Vitro Diagnostics under one or more of U S Patents Nos 5 656 493 5 333 675 5 475 610 clairre 1 44 158 160 163 and 167 only and 6 703 236 clairre 1 7 only or corresponding clairre in their non U S counterparts owned by Applera Corporation No right is conveyed expressly by irmplication or by estoppel under any other patent claim such as clairre to apparatus reagents kits or methods such as 5 nuclease methods Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosysterrs 850 Lincoln Centre Drive Foster City California 94404 USA 5100000 000000000000 000 90000000 LI o L3 r
94. T CA m x m LT T Th 49 5100008 0000000000000 Run sample Mol 25 F L FR IH 1 I 7 00000 00000000 Ud uuu Screen O0 0 0 0 0 0 0 0 0 0000 000 0000 000 0000 0000000 uid Lll 000000000 0000 000000 0 000000000 0000 sedig 0 0000D L Running ITRGOFST Step 1 35 89 B 3808 T emp 23 4 Preheatin3 Li d 56 BB 00000 00000 104 10000 000000000 9810 O 0000000 Running on R ITAQFST Step 1 48 0 0 30 Temp PT 0 Repeat 1 BEEP DELETE DIDI 380000
95. ail life ps jpXabio rad com M 10114 0902A
96. gt gt rm 0 bu bubus Es les es cn m an F ex cl un E BACK ROH 7 5260 0 000020 000000 00000 TEMP 00D UL UO 0000 at 4 3d uid SRAD EHD pan 100 00000000 0000 90000000 0000000 0000 0000 0000000 29 510000 0000000000000 8 III 0000000000000 PCR 000000 0 00 00000000000 0000 IGOTO 00000000 0008 0000000000 U UD gau D 50 Gererm nb uu uuu ENTER TE 000000000000 2
97. lernperature and a regate number decreases the temperature EXT time from 52 to 60 Applies ta both temperature and extendi seconds per cycle gradient teps 30 Instructs the thermal to extend the hold time with each cycle whem a number increasesthe hald time and a negatwe number decreases the hold lime EATE number from 0 1 to 597 Apples only to a temperature step thermal ze cand 31 Instructs the thermal cycler ramp to ramp to te target temperature at the specified ramp rate in that step Apples onk to the Dual 48 and 96 Fast reaction modules EEEP parameters Applies only to temperature step 32 Instructs the thermal cycler as a sgnal that the thema cycler reached the target temperature that step PREVIEW parameters Apples onk to a qradient step Step on page 23 Instructs the thermal cycler to displays the lermperature gradient on the block 71 51000 0000000000000 FILES 0000 0000000000 FILE 1H D DU 0000000 0000 000 000 00000000000000 UI L FOLDERS PROTOCOLS CUPY SECURE M UE HELETE DELETE RENAME REHRHE
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