1-155-606 遺伝子組換え原理探究キット FSB14（取扱説明書）
Contents
1. 3 Feel so Science TEL 03 6277 8041 FAX 03 6277 8042 Mail ed kit leaveanest com2. 70 709 70 70
3. 2 E 4 3 DNA 10 KikG 2 KikGR 2 DNA DNA DNA 5 10 6 42 C 50 7
4. 4 1 2 1 eLB 2 5 1 LB lt LB 1 500 ml 1L 1 70 gt 1 2 2 LB 1 L 300 ml 121 C 20 609C LB
5. 2 1 12 KikG KikGR 2 3 LB Amp LB Amp IPTG
6. 3 16 2 19 7
7. 121 C 20 14 1 30 DNA DNA DNA DNA
8. 7 22 4 KikC KikGR 2 KikG ATGAGTGTGATTACATCAGAAATGAAGATGGAGCTGCGTATGGAAGGCGCTGTAAACGGG KikGR ATGAGTGTGATTACATCAGAAATGAAGATCGAGCTGCGTATGGAAGGCGCTGTAAACGGG KikG CACAAGTTCGTGATTACAGGGAAAGGAAGTGGCCAGCCTTTCGAGGGAATACAGAATATG KikGR CACAAGTTCGTGATTACAGGGAAAGGAAGTGGCCAGCCTTTCGAGGGAATACAGAATGTG KikG GACCTGACAGTCATAGAGGGCGGACCTCTTCCTTTTGCTTTCGATATCCTGACAACAGTA KikGR GACCTGACAGTCATAGAGGGCGGACCTCTTCCTTTTGCTTTCGATATCCTGACAACAGCA KikG TTCGATTACGGCAACCGGGTATTTGTCAAATACCCAGAAGAAATAGTAGACTACTTCAAG KikGR TTCCATTACGGCAACCGGGTATTTGTCGAATACCCAGAAGAAATAGTAGACTACTTCAAG KikG CAGTCGTTTCCTGAGGGTTATTCTTGGGAACGAAGCATGAGTTACGAAGACGGGGGAATT KikGR CAGTCGTTTCCTGAGGGTTATTCTTGGGAACGAAGCATGAGTTACGAAGACGGGGGAATT KikG TGCCTCGCCACAAACAATATAACGATGAAGAAAGACGGCAGCAACTGTTTTGTCTATGAA KikGR TGCCTCGCCACAAACAATATAACGATGAAGAAAGACGGCAGCAACTGTTTTGTCAATGAA KikG ATTCGATTTGATGGTGTGAACTTTCCTGCCAATGGTCCAGTTATGCAGAGGAAGACCGTC KikGR ATTCGATTTGATGGTGTGAACTTTCCTGCCAATGGTCCAGTTATGCAGAGGAAGACCGTC KikG AAATGGG
9. RIK N TT 160 0004 2 11 6 VARCA 10 TEL 03 6277 8041 FAX 03 6277 8042 URL http www leaveanest com a
10. KikG GACCTGACAGTCATAGAGGGCGGACCTCTTCCTTTTGCTTTCGATATCCTGACAACAGTA KikGR GACCTGACAGTCATAGAGGGCGGACCTCTTCCTTTTGCTTTCGATATCCTGACAACAGCA KikG TTCGATTACGGCAACCGGGTATTTGTCAAATACCCAGAAGAAATAGTAGACTACTTCAAG KikGR TTCCATTACGGCAACCGGGTATTTGTCGAATACCCAGAAGAAATAGTAGACTACTTCAAG KikG CAGTCGTTTCCTGAGGGTTATTCTTGGGAACGAAGCATGAGTTACGAAGACGGGGGAATT KikGR CAGTCGTTTCCTGAGGGTTATTCTTGGGAACGAAGCATGAGTTACGAAGACGGGGGAATT KikG TGCCTCGCCACAAACAATATAACGATGAAGAAAGACGGCAGCAACTGTTTTGTCTATGAA KikGR TGCCTCGCCACAAACAATATAACGATGAAGAAAGACGGCAGCAACTGTTTTGTCAATGAA
11. 2 1 PCR Feel so Science 003 DNA 3 2 DNA DNA DNA DNA Feel so Science 004 DNA DNA Feel so Science 005
12. LB 5 3 372C 1 2 70 RT DNA KikG KikGR 11
13. Favia Javus KikGR KikG KikG KikGR 4 KikG KikGR J 4 1
14. NG 4 C KikGR KikG 3 KikG KikGR 10 KikGR 4 121C 20 20 ER 2
15. DNA DNA DNA DNA DNA Feel so science 004 DNA DNA DNA DNA DNA PD DNA
16. KikG ATTCGATTTGATGGTGTGAACTTTCCTGCCAATGGTCCAGTTATGCAGAGGAAGACCGTC KikGR ATTCGATTTGATGGTGTGAACTTTCCTGCCAATGGTCCAGTTATGCAGAGGAAGACCGTC KikG AAATGGGAGCCATCCACTGAGAAAATGTATGTGCGTGATGGAGTGCTGAAGGGTGATGTT KikGR AAATGGGAGCCATCCACTGAGAAAATGTATGTGCGTGATGGAGTGCTGAAGGGTGATGTA KikG AACATGGCTCTGTTGCTTCAAGGAGGTGGCCATTACCGATGTGACTTCAGAACTACTTAC KikGR AACATGGCTCTGTTGCTTCAAGGAGGTGGCCATTACCGATGTGACTTCAGAACTACTTAC KikG AAAGCAAAGAAGGTTGTCCAGTTGCCAGACTATCACTTCGTGGATCATCGAATTGAGATA KikGR AAAGCAAAGAAGGTTGTCCAGTTGCCAGACTATCACTTCGTGGATCATCAAATGGAGATA
17. 1 500 ml 1 100 ml 1212C 20 42C 1 LB 5 DNA
18. 422C 30 DNA DNA DNA B KikG KikGR DNA KikG KikGR IPTG B 1929
19. B B B B GFP Green Fluorescent Protein GFP GFP Kikume Green KikG Kikume Green Red KikGR Favia favus KikG KikGR 480 nm 520 nm 507 nm 517 nm KikGR 360 583 nm 593 nm KikG KikGR
20. 2 10 42 C 50 2 8 LB 250 1 10 9 8 100 LB LB Amp LB Amp IPTG 1 dd 8 LB Amp LB Amp IPTG 10 37 C 16 1 2 mm 4 2 2 gt 2
21. 0 2 1 70 500 ml 37 C 1 429C 1 5 1 1 1 20 ml 5 1 200 il 5 1 5 1 1 1 L 300 ml 1 500 ml 1 1 LB gEscherichia co7 DH So
22. DH 5a 20 C DNA KikG KikGR B DNA 20 C 100 mg ml LB 20 C IPTG B Jsopropyl B D 1 thiogalactopyranoside 25mg ml LB 20 C LB 20 C LB
23. 25plx 2 IPTG 30ul 1 LB 250 mx2 LB 2 5 20 200 ul i 1 1 2 1 37 C 1 1 2 1 Invitrogen E Gel Safe ImagerTM Real Time Transilluminator 1 1 LB 25uu LB LB LB Amp 2 IPTG 50ul LB Amp IPTG 3 1 2
24. DNA 7 4 4 1 5 1 2 1 P1 P1 SS 1 2
25. 1 2 LB LB LB lt LB 1 100 ml 300 ml 1 70 gt 2 9 LB 300 ml 100 ml 121 C 20 250 1 10 e
26. DNA KikGR DNA KikGR 1 16 le Semi rational engineering of a coral fluorescent protein into an efficient
27. 2 3 Ne 4 2 6 7 8 9 20 4 1 2 1 400 pl DNA KikG 60 ul DNA KikGR 60 ul 250 ul IPTG 250pl 1 2 ml LB 100ml 1 LB s00ml 1 20 1 0
28. 2 lt LB 1 1 1 200 200g1 1 70 gt 1 25 LB 1 LB 2 372C 1 w amp LB e 1 2 lt LB 5 1 5 70 gt LB 1 2 1 2 LB LB
29. 8 DNA DNA 7 DNA 1 1 DNA 20 4
30. 5 100 ul 10 LB 250 10 25ul 10 IPTG 50ul LB LB 1 709 1 LB 2 DNA 11 lx 2 100 x 2 LB 250 1 x 2 25ul x 2 IPTG 30ul x 1 1 5 Vv Vv Y 1 1 100 mx2 DNA KikG KikGR 11mlx2
31. 3 4 5 6 21 8 9
32. KikG ACAAGCCATGACAAGGATTACAACAAGGTTAAGCTGTATGAGCATGCTAAAGCTCATTCC KikGR ACAAGCCATGACAAGGATTACAACAAGGTTAAGCTGTATGAGCATGCTAAAGCTCATTCC KikG GGGCTGCCAAGGCTGGCCAAGTAA KikGR GGGCTGCCAAGGCTGGCCAAGTAA 24 KikG KikGR KikG KikGR
33. highlighter Tsutsu H Karasawa S Shimizu H Nukina N Miyawaki A EMBO Rep 2005 Mar 6 3 233 8 2 3 3 lt 2 1 2 DNA DNA DNA DNA
34. AGCCATCCACTGAGAAAATGTATGTGCGTGATGGAGTGCTGAAGGGTGATGTT KikGR AAATGGGAGCCATCCACTGAGAAAATGTATGTGCGTGATGGAGTGCTGAAGGGTGATGTA KikG AACATGGCTCTGTTGCTTCAAGGAGGTGGCCATTACCGATGTGACTTCAGAACTACTTAC KikGR AACATGGCTCTGTTGCTTCAAGGAGGTGGCCATTACCGATGTGACTTCAGAACTACTTAC KikG AAAGCAAAGAAGGTTGTCCAGTTGCCAGACTATCACTTCGTGGATCATCGAATTGAGATA KikGR AAAGCAAAGAAGGTTGTCCAGTTGCCAGACTATCACTTCGTGGATCATCAAATGGAGATA KikG ACAAGCCATGACAAGGATTACAACAAGGTTAAGCTGTATGAGCATGCTAAAGCTCATTCC KikGR ACAAGCCATGACAAGGATTACAACAAGGTTAAGCTGTATGAGCATGCTAAAGCTCATTCC KikG GGGCTGCCAAGGCTGGCCAAGTAA KikGR GGGCTGCCAAGGCTGGCCAAGTAA 23 KikG KikGR 9 8 KikG ATGAGTGTGATTACATCAGAAATGAAGATGGAGCTGCGTATGGAAGGCGCTGTAAACGGG KikGR ATGAGTGTGATTACATCAGAAATGAAGATCGAGCTGCGTATGGAAGGCGCTGTAAACGGG KikG CACAAGTTCGTGATTACAGGGAAAGGAAGTGGCCAGCCTTTCGAGGGAATACAGAATATG KikGR CACAAGTTCGTGATTACAGGGAAAGGAAGTGGCCAGCCTTTCGAGGGAATACAGAATGTG
35. Feel so science 014 1 100 014 1 2 3 4 KikG KikGR 20 28 GFP Green Fluorescent Protein DNA GFP 2008 Kikume Green KikG Kikume Green Red KikGR KikG
Download Pdf Manuals
Related Search