Biacore 1000 日本語取扱説明書
Contents
1. 5 Biacore 1000 Instrument Handbook 62 8 8 Biacore 1 MAIN 1 DEFINE APROG MAIN Method DX DEFINE APROG END 1 MAIN 1 MAIN MAIN2. Biacore 1000 Instrument Handbook 10 1 5 Normalize SPR BlAmaintenance kit BIAnormalizing solution 0 5 ml R2F2 Tools Working Tools Normalize Ta Working Tools X Cancel Lime Rinse canitize Shutdown Standby Normalizes the signal in all flow cells Use this procedure aj when the apto interface is changed b when the temperature is changed c when a new sensor chip is docked and maximum sensitivity is required Total run time is about 5 minutes Last Run Time 12 May 99 15 30 Start Normalize CD
3. JE OANA HE SP e c6REOXS e5X33J2 QU e o Pe
4. GE T 169 0073 3 25 1 TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com ISO 9001 2000
5. OFF TEL 03 5331 9336 ERIT 9 00 17 30 WWw gelifesciences co jp e maill Web 02009 GE
6. DEFINE APROG assay PARAM X 9opos FLOW 20 INJECT Oopos 40 RPOINT baseline b RPOINT bound INJECT R2F3 20 RPOINT regeneration FLOWCELL 1 APROG assay APROG assay APROG assay FLOWCELL 2 APROG assay APROG assay APROG assay APROG assay APPEND CONTINUE Biacore 1000 Instrument Handbook R Il E PR 5 67 gU Lacke M 40 pce oa npe E 18 19 24 aic gd repa e kk kL lt M HS 19 Joder rRNA ERROR 18 at et T2 MM ERE 11 43 BIN E 47 e UM eJ E HH 46 dt ts rN CONN NN A it MR ee 56 ee 47 ev CT RM NRI NER 9 3 19 AR Ae Pp c UT 2
7. 1 Edit Delete Edit gt Insert CAU Io es E Command Queue GI x Fm Edt Hun c QUICKINJECT R2E1 10 4 QUICKINJECT R2E1 10 4 QUICKINJECT R2E1 10 C FLOW 10 Sj MIX R2A1 110 SF FL WPATH 1 2 3 4 4 QUICKINJECT R2E1 10 Command Queue 0 zk Command Queue version Biacore 1000 Instrument Handbook 4 49 4 2
8. RU 200x1 5x 1000 x 1 S x S 50kDol 120kDal d TGC 200 x 1 1x 50 000 120 000 83 RU 75 1000 x 1 1x 50 000 120 000 417 RU XDIBICIS Manual Inject 35 Biacore91000 Instrument Handbook 3 35 3 4 MANUAL INJECT Manual Inject
9. Prime 2 3 2 Flush IFC 3 Rinse FC 20 Jl OND Y Z6 27 V DIGITAL C BLR CC 9 8 3 MilliQ 1 2 OE Prime D Desorb 1 IFCAP 7E ee EE EE TEE BIA BIAdesorb solution 1 0 596 SDS BIAdesorb solution 2 50mM Gly NaOH pH9 5 Thermorack A 2 R2F3 3ml BIAdesorb solution 1 R2F4 3ml BIAdesorb solution 2
10. 2 E Bilayer Biacore91000 Instrument Handbook 1 5 2 Prime Dock Working Tools Prime Working Tools Cancel Shutdown Primes the liquid system by flushing pumps IFC and autosampler with buffer Use this procedure after docking a new chip and when the buffer is changed Total run lime is about 7 minutes Last Hun Time 77 Mar B 18 49 Start i Start Prime Exit Prime D Prime
11. Injection Moe cU UV ee Injection X Injection in Fc 1 2 3 4 KINJECT el Variable Contact Times Sample Position r al 180 amp Consumption 100 ul Contact Time Dissocialion Time dt EES O Recovery Recovery Fraction Collection Mone To Position real Ce Recovery Volume 60 ul O Fractions Extra Cleanup Extra Cleqnup Options Predip Needle Position W Extra Cleanup Biacore 1000 Instrument Handbook 48 4 Command Queue
12. SG ms Rmax Da x RU Da x S s 50 000 Da 1 000 RU 1 PRAN RSE 20 000 Rmax Rmax 20 000 x 1 000 50 000 x 1 400RU 50 100RU 10 000 15 000RU Biacore 1000 Instrument Handbook 34 3 k kJ Kinetics Parameter
13. Tools gt Working Tools Working Tools Prime e Biacore 1000 Instrument Handbook 6 1 10 mM HEPES 0 15 M NaCl 3 mM EDTA 0 00596 Surfactant P20 pH7 4 HBS P 10 mM HEPES 0 15 M NaCl 0 00596 Surfactant P20 pH7 4 HBS N 10 mM HEPES 0 15 M NaCI pH7 4 SESS EJBJIC do4o t2 TIS ZESBIXEECOS et ORT CSL 0 22um 5 C3X8 amp 11UV SERRBUIC T 2 BS C11 2e CM5 Biacore91000 Instrument Handbook 1 7 3 Biacore Command Rack Base Hack Base Rack 1 Lett w Cancel Hack 2 Right Rack Base1 Rack Base
14. NHS INJECT MANUAL INJECT Ju t Ki Run RunSensorgram amp 2 U v 2L 979 727 Bcc 22 6 Manual Inject Command Queue T Command Queue rer FIE Edt Hun Generate Method File Close BIACORE ES 3 Closing the command queue will clear all commands in the queue Do you want to clase the command queue Yese 7 Biacore 1000 Instrument Handbook 36 3 1 NHS NHS EDC i deg st Command Inject i E i Injection Stqrt Injection 7 NHS Biacore 1000 Instrument Handbook 3
15. 50 DNA 150mM GE 2 1 Kier 10mM 12 pH 21 pH SE E gr LI rcs pereo qe eed T c bis A EDCIN ethyl N 5 dimethylaminopropyl carbodiimide hydrochroride 2 NHS N hydroxysuccinimide 8 1M ethanolamine hydrochloride pH 8 5
16. pH pH pH pH5 Biacore 1000 Instrument Handbook 24 3 3 3 E PIER 62 1 Frile Open Open Bl amp core File x eXbiacoreXguideX methods Ex cx etl eu biacore Eg guide ET methods kinetic blm thiol blm TT E54 0108 A BIA Files GI m Ed c 2606 mt3 MM C biacore1000 NguideN methods amine biIm Biqcore bm Method
17. R2A3 6 25ug ml R2A4 3 125ug ml R2F3 10mM Gly HCI pH 1 75 Biacore 1000 Instrument Handbook 4 i 2 Method 28 3 29 4 30 5 32 Ctrl Break 1 Method Resut Resut View Method Method Method Biacore 1000 Instrument Handbook 52 4 4 3 4 2 1 1 Bed E
18. i OK ave Results As ki c Xbiauser cy Cancel C y biauser File name Folders OK ave file as type Drives Results bir sl E c bia Biacore 1000 Instrument Handbook 3 31 immobilization Fe HH Time bshesp Slope Baseline Heen ld mmoh 3025 112237 UU Yes 0 BASELINE mmob 2070 5 14635 4 0 05 Mo 3405 7 immobilization Ctrl Break Method Resut Resut View Method Method Method blim Biacore
19. B Acore x AN Error in method OK de OK gt gt Expected 1 2 3 4 FLOWCELL 5 22 Prerun Method D 2 Run Prerun Method Biacore 1000 Instrument Handbook 30 3 4 Run Run Method qmine bIm EE BIACORE 2 Save changes In method Amine blr No es
20. Lio Biacore 1000 Instrument Handbook quu EP 61 7 Biauser 1 Explore Start gt Program Windows Explores i C QV P774 A2 Biauser File New gt Folder i Enter 2 My computer BIACORE control software My computer gt C Biauser e File gt New gt Folder Enter Je Biauser Biacore
21. NHS CONH COO COO CONHCH CH OH 08 2 50 2U 77 I Ez o2 2 0237 FOXIBICTS ES SISBERI 7 Vi amp e fT CHEI 7375 D S5 ZSE ZI ivo 9 gU OE t I EZUE Biacore 1000 Instrument Handbook 3 19 SA DNA DNA DNA SE ST C TEMERE C 1 10ug ml
22. n 28 p ul uro 2 de 55 ml Ls pn 7 8 See 18 J ja HF STR cierre UNI HM POPE UP 20 Ne EE 18 J SE EE 18 24 ME 14 Le dd cna ML UM ED tau Ate eL O E UE 55 RR 14 66 INDEX A Aprog DR E Ee ee eege EE 63 B BIGINJECT E I MU RD LI EE ODIT OIL ES 45 B1 4 C W ISSE 4 COINJECT PITE IERI T ODIT M IU IP CY ECT DD BU I DUBII 45 CONTINUE EE 5 3 Command Queue eH TCR P 48 C1 F SS EE EE MM IEEE AD RENE NE 4 D Desorb SUN ee E T P EE E EE ES EE ee ENEE ER EE PT E EE 55 DNA Tm M 20 19 Dock EE 3 E Extra Cleanup dE E A Ee dE EE 47 F D p TE 4 FLOWCELL UH Se 92232RSSSeS202die3 FLUSH Ge Ee ele deed e 55 H HEPA 4 I INJECT A EE E EE EE EE 11 43 47 K KINJECT cececsooooooocosoooooocoosooosooosoooosoocoosocosocosooocosooosoocoosoosooocsoososoocsooocosocoosoocosooosoocssocossoocoocossooosocoosoocosooosoooossooosoooo
23. SA NHS pH8 5 TSEURCU C 10750mM POT pH 85 100ug ml 150mM SA Ian d 2 Biacore 1000 Instrument Handbook 3 21 3 2
24. CM3 CM5 E 227 235 C1 CMS EB DNA NTA NTA His Tag Fusion Protein Ni HPA C18 Monolayer L1
25. 30 i Biacore 2 mp valo Biacore 1000 Instrument Handbook 2 1 2 Biacore BIA Programs Ta BIACORE 1000 Control Software 2 O amp J5lAcore 1000 Control Software on COM KINETIC Sensorgram RriSiiee Je a x Edit View F F TE Se er cdi a xj kala A5 5i us D23 Ye BASELINE kinetic 5 62 Dei D o i BASELINE 25 Heportpont table kinetic 55 i 24 4 28 Di re BASELINE lt kinetic H 1712 2B AN res BASELIIE kinetic Gah 1 83 Y Tr 1 E xu Erd 2 Te le Tima 979 8 x Finzponzme 118145 AU Fire Calit SUME siroke Menu bar BIACORE Toolbar
26. BEAR 46 CD pH 3 5 30 1 GE Sch Biacore 1000 Instrument Handbook 42 4 4 1 Jeux Run Run sensorgram Fm i TRYIS Flowcell Flow Cell e Fc 1 cl Fcz cl Fcs OK Ka kg 20 50ul min VAN Command Inject Biacore 1000 Instrument Handbook 4 43 I
27. DEFINE APROG injection FLOW 5 INJECT R2A1 5 END MAIN FLOWCELL 1 APROG injection END MAIN APROG injection DEFINE APROG injection APROG MAIN END DEFINE APROG MAIN APROG Biacore 1000 Instrument Handbook 8 65 APROG MAIN APROG injection APROG injection 2 DEFINE APROG injection FLOW 5 INJECT R2A1 5 injection injection APROG injection doc DEFINE APROG injection FLOW 5 INJECT R2A1 5 END MAIN FLOWCELL 1 APROG injection FLOWCELL 2 APROG Injection FLOWCELL 3 APROG Injection FLOWCELL 4 APROG Injection Biacore 1000 Instrument Handbook 66 8 AJ Dub
28. UT ERRI De OK 8 Biacore 1000 Instrument Handbook 8 1 Thermo_A Thermo_B Thermo_C 3 Iz 96 0 seq 2 OMVIG Thermo A EMO 9 mm 0 5 ml 16 tint 29 2 Dell Thermo B 9 mm 05ml Thermo C 2 15 mc US qr MICRO 967700 sc T 2 EOS e Rack Basel R1 Rack Base R2 or 1 3 R1A1 R1A2 R2A3 f 2
29. 10mM pH6 100l 10mM pH5 100bH 10mM pH4 100bH A A ip uie TN 150mM HBS 50mM NaOH 30 Biacore 1000 Instrument Handbook 22 3 5 50ug ml 10mM AFARIREN pH6 100u 10mM BERE pH5 100ul 10mM BERTI C pH4 100 i ZU BD X Run gt Run Sensorgram Flowcell Flow Cell Cancel OK
30. Biqcore Bulk effect D 0 0 baseline bound Window 2 Delete Biacore 1000 Instrument Handbook 2 2 3
31. Fc AFROG Time bsAeszp Slope Baseline RelResp ld Blank Ligand ame Target 677 5 117706 0 0 01 Yes DU Baseline anti alb1 Hunrmll 3 Am4 12405 1317823 0 12 Mo 117 2 EDC NH5 No antr alb 1 ugZml 1000 3 Am4 1511 5 187342 0 02 Mo 1028 3 Ligand Ho anti alb 1 ugZml 1000 3 Am4 2151 5 18735 3 0 19 Mo 1030 3 EIhannlamine Mo antr alb 1 ugZml 1000 Biacore 1000 Instrument Handbook 40 4 A crude
32. File gt Save As Save Results As x File name Folders HK Cancel Gd c CH biauser Cd name Dave file as type Drives Results bir M amp c bia C biquser OK a 71 gu 61 Y gt 0N Biacore 1000 Instrument Handbook 17 18 3 N 3 90 CM5 CD EPA E N g CM NHS N
33. O Biacore 1000 Instrument Handbook 2 11 2 Biacore Sucrose SPR 296 Sucrose 496 Sucrose 8 Sucrose 16 Sucrose 2 1 100 JU EX Run Run sensorgram nisi d tees Cancel Flow Cell OK Flow X Set Flow Pow 0 wm Refill Cancel Biacore 1000 Instrument Handbook 12 2 1 100ul
34. 2 1 Continue Tools Working Tools Continue 5ul min 72 Continue 72 Continue som 2 Biacore lt 2 1 MilliO Prime REDAN P JU E MilliQe2K7h P JU eei Tools Working Tools Prime 2 Close 16 mm MilliO 3 MAN R2F3 Tools Working Tools Close Biacore91000 Instrument Handbook 54 5 5 2 Undock Command Undock
35. EDC NHS 10ml MilliQ 200ul 0 5ml 7mm Z2 20 B35 1 4C 200ul Biacore 1000 Instrument Handbook 20 3 2 5 200ug ml 10mM pH 1 2 pH Z2N 21 eb Ed nU pe b tese 10mM pH3 5
36. Sensorgram window i nec Hc q Report point table Eventlog window CW Status window RU Run Biacore 1000 Instrument Handbook 1 3 1 2 1 Dock Dock Dock Insert a new sensor chip Check Dock when ready After docking you should run Prime from Working Tools 4 0 0 E Sensor chip Dock Dock Sensor chip
37. MAIN EP C Ea ES o MAINCINSU 2 70090 0 7 E12 2 Ix amp d EROR OEUS s File New Method Method NIIF S MAIN FLOWCELL 1 END Run gt Run Method Status window FLOWCELL 1 Nc MAIN Run MAIN FLOWCELL 1 FLOWCELL 2 FLOWCELL 3 FLOWCELL 4 END Run FLOWCELL 1 gt 2 gt 3 4 MAIN Biacore91000 Instrument Handbook 8 63 MAIN Working Toos Run Prime 2 Rinse 2 FO
38. EILAN ORAAL 91 5 3U 977 0u 3 WII 1 OHE HITA Ab IC 60 Lil a5 H S d H A d 8Bila 2 FRP CRUS VN i EEBOIBNICIAU x o o iB
39. ee 0 pes 2 0 7 DJ DEFINE APROG assay FLOW 20 INJECT R2A1 40 END MAIN FLOWCELL 1 APROG assay APPEND CONTINUE END R2A1 1 3778 20 25 clc AR R2A1 R2A2 R2A3 DEFINE APROG assay PARAM 9opos FLOW 20 INJECT Oopos 40 FLOWCELL APROG assay APROG assay APROG assay APPEND CONTINUE Biacore91000 Instrument Handbook 8 73 Biqacore INJECT 9 pos 9 pos PARAM pos MAIN 7h APROG assay 73 45 0 C APROG assay APROG assay R2A1 R2A1 POS 2
40. NHS EDC NHS 30ul 100u INJECT R2F3 10 RIFS UNA SA tae tqmone dst EE E E 10mM Gly HCI pH 1 52 0 10 50mM NaOH 1 1 Biacore 1000 Instrument Handbook 3 27 3 21 So fGGUL Ce UFC Ce gd DENTE 4 C Nbiacore1000 Nguide methods s eos
41. RUD 0 OU N 5 14 APRO G FC Time window AbsHesp SD Slhpe Baseline HelHesp ld kinetics H ZOU 50 23rar 1 D60 016 Yes 0 baseline kinetics 4 335 0 20 2450 5 5 39 6 78 Ma 7135 bound CORIN t iuur ed es DEFINE APROG Injection FLOW 5 0 10RPOINT 10sec 0 20 RPOINT 20sec 0 350 RPOINT 30sec END MAIN FLOWCELL 1 APROG injection END APROG RPOINT 0 10 RPOINT 10sec 10 Biacore91000 Instrument Handbook 8 67 Time Windnw AbsHesp SD Slope Baseline FielFiesp Id 10 5 50 324306 6 1115 50 20474 121 5 50 345
42. assay blm 62 1 Jw t File Open Open Bl amp core File x ceXbiacoreXguidexX method Ex cx evtl Se Gees CN guide Gs method mf T E54 0080 A Files Gk bl T E c 2606 mf3 h C biqcore1000NguideNmethods assay blm OK Biacore 1000 Instrument Handbook 50 4 DEFINE APROG assay PARAM 96pos 96id 96conc CAPTION Binding of 9 ID conc to immobilized antibody FLOW 20 KINJECT pos 60 180 RPOINT baseline b RPOINT bound QUICKINJECT RZF3 20 RPOINT regeneration RACK 1 thermo b RACK 2 thermo a flowcell 1 APROG assay R2A1 Sample 50ug ml APROG assay R2A2 Sample 25ug ml APROG assay R2A3 Sample 12 5ug ml APROG assay R2A4 Sample 6 25ug ml flowcell 2 APROG assay R2A1 Sample 50ug ml APROG assay R2A2 Sample 25ug ml APROG assay R2A3 Sample 12 5ug ml APROG assay R2A4 Sample 6 25ug ml APPEND continue IContinuous flow after aprog EE 0 Spe 2b e
43. ze pu 4 5 do E Weg EE Biacore 1000 Instrument Handbook 14 2 22 QU View Reference Line IRIA ag c Se PJ PF lt T i i Edit Add Report Point
44. Sqnitize MiliO Prime 2 3 6 2 Air zl TVA EEA ERAL EE TH I Amine Coupling HSA 10ug ml Biacore 1000 Instrument Handbook ZE D Tools gt Working Tools gt Prime Flush Rinse 2 Tools Service Tools Unclogging O Prime m EK S1 ASNA D NANS S120 RREA IFC p A US O 3 e EE Rabbit IgG 1 25hg ml Tools Service Tools Unclogging
45. Biacore 1000 Instrument Handbook 4 1 Dock er 0 60 Undock Sensor chip Doch e5 SEU HS d Slc S S 1 FA Research Grade 15 Certified Grade 5 CM4 CM5
46. i Flow Set Flow Refill Cancel G 100ul min 10ul min OK t LA Command gt Inject Y D Stqrt Injection Biacore 1000 Instrument Handbook 3 23 Injection Injection in Fc 1 GN 5 INJECT S ampls L ancel Boston r2al hl Consumption At ul Contact Time i Hore gt gt i Preconcerntratian nf mousc Ist pH P MouselgG i e 7 Zee PIZ Run Stop sensorgram amp 7 U V2 UL GIBEERE S S S pH pH NHS pH4 5 pH4
47. Undock Sensor Chip i Dock File Exit I l Biacore Off 5 3 Undock Z 1 Dry COAR 50ml Sae TE 4C DNA 2 Wet PBS 50 ml 4
48. 37 2 Ju T LA Command gt Inject T3 Injection Injection in Fe 1 le INJECT sl Contact Time Consumption i More gt MANUAL INJECT Start Injection i 7 9 am Mamual Iniection Mode FIT EGIT C v ilume Ge enu Accumulated injected volume 0 pl Remalnng volume in pump 42 pl Cancel Load Loop From Position E bd Volume su ul Predip Needle Position T 2 120ul OK Z 30ul Cancel Biacore 1000 Instrument Handbook 38 3 d Manual Injection Mode xc Injection E Start C Molume ul ze Ce Manual stap Accumulated injected volume D pl FLLLLLLLLLI KIT Remaining volume in pump 382 ul Eat Manual Start Stop 1 gt 100ul GG es sg OD a E
49. R2F2 96 7 Biacore CO D 5cm Biacore 1000 Instrument Handbook 1 9 4 Command SetTemperature Det Temperature Set Temperature Temperature Sb Cancel 20 37C OK 20 37 states window H MAO Temperature ODI 7 Z7 9 TRES OD LC do
50. Biacore 1000 Instrument Handbook 58 S aS Ara RUP 6 4 CM5 HBS EP 9mm BIAtest solution BIA maintenance kit BIA maintenance kit BIAtest solution 1ml amp 9mm R2F1 2 Thermo_A 9mm 4 R2E1 R2E4 Tools Testtools System check Test lools X System check stat System check evaluation IN Cancel Checks detection unit flow cell factors pump function autosampler performance and IFC sample loop and valves Total run lime is about 40 minutes NOTE This procedure should be run at 25 C with a new Sensor Chip CMD Last Hun Time Start
51. 2 7 CM CM pH 1 2 pH pH pH 5 50ug ml
52. Run Stop Sensorgram 14 10 10 430 30 1 BlAevaluation Biacore 1000 Instrument Handbook 4 45 Qm NaCl 10mM Gly HCI HCI Phosphoric acid Formic acid 10mM Gly NaOH NaOH Etanolamine Etanolamine HCI L R 210722 4 7 t Ee IS OG EDTA Surfactant P 20 Tween
53. MERA 22 Biacore 1000 Instrument Handbook 56 S aS Ara RUP Biacore Desorb 20 BIAdesorb solution 1 4 eot Desorb MiliO Prime 2 3 Saqnitize 1 FC BIAdisinfectant solution MilliQ 1 1 5ml BIAdisinfectant solution BIA 20 ml MilliQeZK CHRL 27 V TREAD SIC iS e SE 2 MIlliQe2K 15ml amp 45 Sanitize
54. Bulk Effect 46 ng ml ud ml kop 1 10 10 RU 18 miye T 12 d 7 100 200 300 400 500 600 Time s Biacore 1000 Instrument Handbook 4 41 Regeneration O
55. R2A2 3 R2A3 APROG assay UEZSSBLCU X JST eos DEFINE APROG assay PARAM 9opos FLOW 20 INJECT Oopos 40 RPOINT baseline b RPOINT bound INJECT R2F3 20 RPOINT regeneration FLOWCELL 1 APROG assay R2A1 APROG assay R2A2 APROG assay R2A3 APPEND CONTINUE Biacore 1000 Instrument Handbook 74 8 2 2 2 tI uibs R2A1 R2A2 RAS 3 2079 16 204 30u DEFINE APROG assay FLOW 20 j IN
56. Add R eport Point Sensorgram Fc 4 DE Time e pw Window amp Cancel T Baseline Apply to all curves in window Biacore 1000 Instrument Handbook 2 15 ld 0 Baseline RelResp 0 Add Report Point Sensorgram Fc 4 Time Window z i Baseline I Apply to all curves in window RU Injection of several concentration of Sucrose 17000 15000 15000 14000 o eo a ZS 13000 12000 11000 10000 RelResp i Biacore 1000 Instrument Handbook 16 2 Injection of several concentration of Sucrose 0 100 200 Fc Time AbsHesp Slope Baselne RelHesp ld 1 855 124353 0 06 Yes 0 baseline 1 1275 154229 0 02 No 2927 0 2 sucrose 1 2985 18309 7 0 08 Mo 5813 7 4X sucrose 1 4 25 24010 1 1 24 No 11514 1 OS sucrose 1 648 5 353049 1 32 No 22808 3 15 sucrose E508 OD AZ LC IT LIE UL ATRA BS STi S
57. Dock Dock Biacore 1000 Instrument Handbook 6 6 1 Biacore Working Tools Tools Working Tools WU T OR CIT SS 1 R amp I OR Prime Dock EB m 7 V ZTRGTAS el BH C TT 2388 sv WIE MERAN 6 Prime
58. 20 30 0 RPOINT b 0 10 RPOINT 10sec b 0 DEFINE APROG assay FLOW 5 x INJECT R1A1 5 RPOINT 10sec RPOINT 20sec RPOINT 30sec Time window AbsResp 101 5 50 334306 6 10827 3B 111 5 50 343 WA 1435 T 1215 50 hj 524 278 No 1365 5 Jec Biacore 1000 Instrument Handbook 4 8 69 C Nbiacore1000N guideN methods amine blm DEFINE APROG immob CAPTION Amine coupling FLOW DILUTE INJECT RPOINT INJECT INJECT INJECT RPOINT RACK 1 RACK 2 FLOWCELL APROG APPEND 10 R2E1 R2E2 RZE3 50 R2E3 70 Baseline b R2A1 70 R2
59. EEN 35 4 40 2s x e Ce ER de AEHETTEREU E 42 UEM NECESE leet e E 49 1 HD eee 49 2 Method MR RE 51 3 c Y 51 aT NN 51 SPATTEN eebe 51 2 52 5 Eeer 53 ZI 3 Meteo ous EE kG 53 5 2 L Undock See ee ee 54 5 3 Y M 5A E NRN 55 55 6 2 56 e CE a e SN E 57 58 T i ET NI SE 61 1 Explore M 61 2jJMy Computer oo el 8 2 NO00 WE 62 Cg 3 34 1 WIS LA ME T A NSS ET 62 ee APROG 63 3 UIR KIRJ 66 LOU EE i 69 Nurs c TEM SE e Ee GE 72 1 1 1 1 1 1 00315 E d d ORRGDRLOD E ER gt gt gt gt d
60. Biacore91000 Instrument Handbook 28 3 2 Method AROJ A E Ud IE NIIS Fels CC Edit gt Adjust Method Second Minute define aprog immob caption immobilization flow 5 dilute r2el r2e2 r2e3 A inject 2e3 35 0 10 rpoint baseline b end Edit gt Adjust Method gt Minute DEFINE APROG immob CAPTION immobilization FLOW 5 DILUTE r2elr2e2r e3 P INJECT 2e3 55 0 10 RPOINT baseline b END METHOD 1 2 Second Minute Biacore 1000 Instrument Handbook 3 29 3 Run Prerun Method i
61. 50 Biacore 1000 Instrument Handbook System Check To evaluate the System check click the Evaluate button Evaluate Biacore 1000 Instrument Handbook 60 S aS Ara RUP BIACORE 1000 System Check Mame Crpabei By BI amp CORE 1000 Conta Software IF IE 20 7 gea Insirumernr rE delt at ECG Zeth Herpere Pun Dale 22 Mar 1 035 Eaa Date 12 Sep 1539 Arabais ieme 25057 RESULTS A IFE Smia Feit 22165 Fe gems FEX z2 Ei 22461 FLA 214p Leaks 34 s22225 H Hefractametnr tesi 155 Sume Ft Fc lc d FE 4 Loumgterm Sope ELT Fc t DL 045 Fils c03 RUD Fc 2 BZ 0405 Fils ET AU Fc X 0 00 1 05 Ris
62. MAIN 1 2 APROG MAIN Rn MAIN APROG Analysis Program cc cles DELI NEAR OL TT EC NED RATE TI I ER CO LE kt DEFINE APROG injection FLOW 5 INJECT R2A1 5 END DEFINE APROG injection injection APROG FLOW 5pl min Run gt Prerun Method error Prerun Method 29 Biacore81000 Instrument Handbook 64 8 gt gt Expecting keyword MAIN MAIN APROG MAIN APROG
63. min OK d gos X Command gt Inject Injection Injection in Fc 1 Cancel Start Injection 5 ample Position Volume ul 60 amp Consumption At ul Contact Time Hore gt gt FY LORYXYY IYRES 10ul Start Injection Injection of several concentration of Sucrose co o E o Q co cc 296 Sucrose i J Biacore 1000 Instrument Handbook 2 13 RU Injection of several concentration of Sucrose 40000 35000 30000 25000 Response 20000 15000 10000 5000 100 200 300 400 500 600 700 a00 Time s Fc Time AbsResp Slope Baseline RelResp Id 1 85 5 12495 9 0 06 Yes 0 baseline 1 127 5 154229 0 02 No 2927 0 2 sucrose 1 298 5 18309 7 0 08 No 5813 7 4 sucrose 1 4725 24010 1 0 24 No 11514 1 8 sucrose 1 648 5 35304 9 40 92 No 22808 9 16 sucrose Run Stop Sensorgrum
64. 0 11 31 Wu NP EE 25 74 EEE SE Br NNE IE EG 35 GENEE dee EE EE E A E Ne 2 41 45 doc ee c 56 M SA 20 46 EE 47 cce EE ccu CM NAT 58 i 3 T PHo n Y 53 E nord y SN ES i AER 4 ee ac Up E 090757700000001000000 277077 20700717700 0727007001071 711 0 3 54 EE 54 EE e E 42 JEEP SU EE 49 TAE EHTEEROO e Ee 50 72 EE 1 2 FI E EE 18 ER DE E EE E T NN OTT T ETTE E 20 EE 61 GE Ee E 1 OO 10 La NT ER eom NN NC augur eae temas it Uude utc p En pOM dd 4 UI 12 52 BI Wii P EE NE 55 EXP ERU p ug p 21 pi CE 29 EE RE 30 SCH RE 20 Pin AN 62 JOZ RW anaE aS e 49 AR 15 68 2 UN EN ESE NE EE 20 wore sp Ee ee 35 AA xw HR 62 s EU
65. 03 3 RPOINT 10sec 20sec 30sec Id AbsResp RelResp GB 5 Window 5 Ck DEFINE APROG assay FLOW x INJECT RPOINT 0 20 RPOINT 0 30 RPOINT INJECT 0 10 RPOINT 0 20 RPOINT 0 350 RPOINT END MAIN FOWCELL APROG END Biacore91000 Instrument Handbook 68 8 0 10 RPOINT 10sec x INJECT R1A1 5 R1A1 10
66. 1000 Instrument Handbook 32 3 5 5 Continue MAIN APPEND CONTINUE Continue Continuous flow for 72 hours Stop this procedure by pressing Stop Stop Continue Biacore 1000 Instrument Handbook 3 33 CO 8
67. 180 RPOINT Baseline b RPOINT bound INJECT ReF5 20 RPOINT regeneration FLOWCELL 1 APROG assay antigen 1 25ug ml APROG assay antigen 1 12 5ug ml APROG assay antigen 2 25ug ml APROG assay antigen 2 12 5ug ml FLOWCELL 2 APROG assay antigen 1 25ug ml APROG assay antigen 1 12 5ug ml APROG assay antigen 2 25ug ml APROG assay antigen 2 12 5ug ml APPEND CONTINUE Biacore 1000 Instrument Handbook 76 8 9 ID conc CAPTION Binding of 9 ID 9 conc to immobilized antibody ID conc soe 1950 7 7 eld o m Binding of Antigen 1 25ug ml to immobilized antibody CR 0 550319 JL TRES Binding of Antigen 1 12 5ug ml to immobilized antibody OCN 0
68. 20 Triton X 100 SDS Octylglycoside Acetonitrile DMSO Ethyleneglycol in HBS buffer Ethanol Formamide Guanidine HCI Urea pH lt 1M gt pH1 5 lt 100mM lt 100mM lt 2096 lt pH12 lt 100mM lt 100mM lt 1M lt 0 35M lt 5 lt 5 lt 0 5 lt 40mM lt 20 lt 8 lt 50 lt 20 lt 40 lt 5M lt 8M Biacore 1000 Instrument Handbook 46 4 VPE a Bulk effecd Response 1 1 lt comnand step Iniect WASTE Injection Stopped L Remaining 5 ample H Dispense remaining sample to Biacore 1000 Instrument Handbook 4 47
69. E4 70 R2F3 10 immob thermo b thermo a 1 immob CONTINUE MAIN RACK 1 thermo_b Command Rack Base Biacore 1000 Instrument Handbook 70 8 APROG CAPTION Amine coupling coq 0 9 FLOW 10 10ul min DILUTE R2E1 R2E2 R2E3 50 NHS EDC R2E1 EDC R2E2 NHS R2E3 50 R2E1 50 eg 200ul INJECT R2E3 70 R2E3 70 10u min 7 INJECT R2A1 70 R2A1 70u H
70. ESE INJECT R2E4 70 R2E2 70u NHS INJECT R2F3 10 50mM NaOH Biacore 1000 Instrument Handbook 8 71 MAIN FLOWCELL 1 1 APROG immob APROG immob APPEND CONTINUE Continue 5ul min 53 APPEND CONTINUE Immobilization Fc APR G Time AbsRHesp Slope Baselne HelHesp ld immnh 3125 119550 002 Yes 0 Baseline 2 nmmnh 271235 3151202 0 23 Wo 3154 2 immob Biacore81000 Instrument Handbook 72 8 5
71. GE Healthcare d Biacore Biacore 1000 Instrument Handbook GE imagination at work 71 3200 31 Biacore 1000 GE 3 25 1 TEL 03 5331 9336 FAX 03 5331 9370 E mail Tech JP ge com PME CAU ACTA Md er E 1 EHS Eed d fe Ae Eer EE 1 Qm gud den a uq 0 UI 1 2 Biacore 3Y FO JUV 2HR 0T PODES eee emm meme ene 2 1 2 E PS 3 1 Dock J 098 AN M 3 APE RRB 5 7 A c coe odd M M nM iMd edi p nS 9 illegale EE 10 P E 11 PIPER 11 ZA EAE ME NODI E 14 2 999 TE 17 3 G 18 3 1 P Tu A be P 19 2 2 LA PIRE emm 21 2 954774 24 1 24 2 Method KEREN EE E EENEG 28 LM D A SE ESS EE NE ET EE 29 ANAL E PINO a NE 20 570 DDN ZE 32 3 4 MANUAL INJECT
72. IACORE 2000 Control Software on COMI Kinetic Sensoregram kel File Edit Miew Gomrmand Run Tools Window Help kl 18 xl pc Ex m x nit Se ie E CH C 1 sl urve 5Sensorgram Fc 1 eif Dr adesfo 400 5 5 500 1000 Time AFROG Time AbsRHesp Slopg Baseline Rieliesp Id mh L kinetic b3 59 z441 2 A es I e e SUB 0o o o o oos E poss a De on Ep UM M E ix 1 kinetic 43555 193071 7 0 03 res 1530 5 ligand 286 1 kinetic 646 5 19221 0 39 Yes 149 6 analyte 286 1 kinetic 8765 5 17595 0 16 No 1625 BASELIMEZ 286 E vent Log Time Info DO Timestamp 95 017 08 59 49 UU Set Temperatu 25 0 Nc Flow Flow Cell nn Temperature Ue ns JIC S Flow Cel 3 4 Temperatur Dn Flow 20 Kimin Instrument Offline DU Data Cgafecthon 1 Hz cyde 1 Cycle Biacore 1000 Instrument Handbook 5 53 5 5 1 EROK I 1 Continue 2 Hautes Cs 1 Continue 3
73. JECT pos vol 0 10 RPOINT baseline b 2 20 RPOINT bound 3 INJECT R2F3 20 2 00 RPOINT regeneration END MAIN FLOWCELL APROG R2A1 10 APROG R2A2 20 APROG APPEND CONTINUE pos vol 2 PARAM pos vol INJECT 96pos 96vol MAIN APROG assay PARAM 9 6pos vol 4 Ag 1 R2A1 10ul APROG assay R2A1 10 2 R2A2 20l APROG assay R2A2 20 3 R2A3 30ul APROG assay R2A3 30 Jg APROG PRAM Biacore 1000 Instrument Handbook 8 75 EL je ELTE CDAntigen 1 25ug ml 2 Antigen_1 12 5ug ml 2 2 Antigen_2 25pg ml DAntigen 2 12 5ug ml DEFINE APRO dssQg PARAM 9epos ID 96conc CAPTION Binding of ID 96conc toimmobilized antibody FLOW 20 KINJECT pos 60
74. NJECT Injection L Injection in Fc 1 2 3 4 KINJECT el Variable Contact Times Sample Pozitian r2al 180 amp Consumption 100 ul Start Injection Cancel it Contact Time i Hore gt gt Dissociation Time KINJECT INJECT KINJECT 10 250ul QUICKINJECT COINJECT 2 Sample 1 10 100yI Sample 2 15 250Ll BIGINJECT 325 750yul 52Ll MANUAL INJECT Biacore 1000 Instrument Handbook 44 4 E o o Q tc 45 INJECT Response
75. blr Result ble BlAevaluation software Biacore 1000 Instrument Handbook 3 25 PESO LIO SRSD mcs ties DEFINE APROG immob CAPTION Amine coupling 0 6 FLOW 10 10ul min DILUTE R2E1 R2E2 R2E3 50 NHS EDC INJECT R2E3 70 NHS EDC RPOINT Baseline b INJECT R2A1 70 INJECT R2E4 70 INJECT R2F3 10 RPOINT immob RACK 1 thermo_b RACK 2 thermo a FLOWCELL 1 APROG immob APROG immob APPEND CONTINUE CONTINUE R2A1 R2E1 R2E2 R2ES R2EA R2F5 EB pH NHS EL Id EDC 7 9887 EK U BB US REB TS 0D 6 02 EDC NHS 7S48I87J DV UI SS TS 06 02 9 mm 0 5ml v4 207 a 2 Biacore 1000 Instr
76. l View Bqseline 0 E Cc kg File Edit View Command Run Tools Window Help Gi Tn e i Hlp ei Cycle m j Curve Sensorgram Fc 3 RU Response 17000 15000 15000 1100 1150 1200 1250 1300 1350 1400 Time Fc APROG Time AbsResp Slope Baseline RelResp Id Blank LigandName Target 3 Aim4 495 177221 0 01 Yes 0 TestiBase No anti alb 10ug ml 1000 3 Aim4 71 5 16878 7 85 14 No 843 4 Testi 1 No anti alb i ug ml 1000 9p N Event Log r Sensorgram Fc 3 Time Info Time 1360 55 Rel Resp 3968RU 0 0 Rack 1 Left Thermo C b 4 0 0 Rack 2 Right Thermo A Flow Flow Cel gl 00 Rack R Reagent REAG A i 00 Flow Cek 3 Leners E 00 Flow 5 I l min Not Connected to Instrument 00 Data Collection 1 Hz Biacore 1000 Instrument Handbook 3 39 3 NHS EDC INJECT NHS EDC
77. osoocoocoosooosooooooo 43 L 1 4 M Main 0 25 50 62 Method mr EE Ee EE EE EE EE EEE T 28 N Normalize UCM IERI SE OE T LLLI DIDIT M 10 NTA 4 P Prerun Method p 29 PRIME EEE EEE EE EE EE EE Ek 5 55 Q QUICKINJECT E E EE E A A E EE E EEE 43 R Rinse NEE E ASEE A E DFE dE EE 55 S AT E 4 Sanitize 56 U Unclogging 57 Undock p PT TR EE 54 XD coUv duUlcla CSL Ed EE EE rine nad o Pae Is U Een em i IC fees Geneck D 9 Di a d Ka on o m
78. ument Handbook 26 3 62 FLOW 10 10ulmin DILUTE R2E1 R2E2 R2E3 50 R2E1 RE R2E3 EDC NHS 200ul 50 R2E1 50 INJECT R2E3 70 da L icikgeer7l dL77r7ku t amp t79U xXme HUS ae 7 7 CM 40 fy 10 INJECT R2A1 70 R2A1 70ul 7 30ul 100u INJECT R2E4 70 R2E4 1 M 70ul
Download Pdf Manuals
Related Search