Biacore 3000 v4 ウィザード版 日本語取扱説明書
Contents
1. CN hm Pe2. Biacore 3000 38 4 17
3. 60 4 Z 2 a O O
4. OFF TEL 03 5331 9336 ED 9 00 17 30 TEL 03 5331 9315 FAX 03 5331 9349
5. DERIEET ET Hi UCEIRIEECT 1 1 LCEINIE ET HE HE FL a HE HA 1 FLUWPATH 1 4 PEIRMEET 1 1 Edit Delete Edit Insert 24 lt Command gt Flow tt Fmm Be mm Bem 25 Command gt Flow Path Im Fuh Tire bein tend TA Iret rr rtt lrese r rg en rim FE4 Fr Fe 4 OK Biacore 3000
6. 36 Cycles ET
7. Biacore 3000 114 9 Surface Prep 46 MS NaCl Surfqctqnt P20 DMSO Tris HEPES MALDI 20 mM ESI 1 mM
8. 1 1 Langmuir with mass transfer Biacore 3000 60 4 4 3 2 Linked Reaction 1 1 1 1 Run gt Run Application Wizard Kinetic Analysis Mr EE FE EL tl Ba i a i ry ee l
9. Rmax 50RU Rmax x xS Da 50 000 Do 1 000 RU 1 20 000 Da Rmax 20 000 x 1 000 50 000 x 1 400 RU 10 000RU RU vs C ks k
10. 0 5 TFA MALDI 0 1 ES ZipTip Milipore e g 50 mM NaoOH 47 2 2 ul
11. Sensor chip Dock Dock Sensor chip 3 Dock Undock Sensor chip Dock Biacore 3000 1 4
12. 5C 1 Biacore 3000 10 1 1 2 5 SPR Thermo A 2 BIAmaintenance kit BIAnormalizing solution 0 5 ml 9 mm R2F2 Tools Working Tools Normalize On Tole Tioial nm tie im aboad S minutes LaWFRamTimw 13HMmr99 1530 Start 8 Normalize SPR
13. mimm Capling EMHTHug ml Tools gt Working Tools gt Prime Flush Rinse Tools Service Tools gt Unclogging IFC 7 4 Biacore 3000 98 7 7 3 Rabbit IgG 1 25ug ml Tools gt Service Tools gt Unclogging 7 4
14. 8 CM5 HBS EP 9mm BIAtest soluton BIA maintenance kit Desorb Saqnitize Dock HBS EP Prime 2 Thermo A R2F1 BIAtest solution 1ml 9 mm R2E1 E2 E3 9mm Tools gt Testtools gt System check Biacore 3000 7 CH uF Pa mLsmuF 1 mermma LN Ek Em um a Si mh Fm Fer Cg LRunTma Feb 1 1 Start 40 4 A A amp A A A 4A 4 4 4 500 1000 rh mm em heh eh he Ebi ear
15. Surface Preparation Run gt Run Application Wizard Surface Preparation Start EE HE a FT Surface Performance Test Next gt it FF FE tT LE tin EE re eg In Ta eml Ek ate Fer lh He cjk mw gm Name Injection Time Flow Rate Number of Test Cycles 5 Use Flowcell Next gt Biacore 3000 Regeneration Method Dissociation in Buffer Single
16. 1 1 Protein A 2 3 2 se Solution Vary by Cycle Antibody1 OK 2 2 e em mmm Solution Antigen Vary by Cycle OK Solution Vary by Cycle Antibody2 OK Biacore 3000 5 83 a i eee 1 la La NE a a LN IM II a Antibody1 Antibody2 Biacore 3000 84 5 34 Customized Application C Program Files BIACORE3000 Guide Methods
17. HBS PBS HBS EP 10 mM HEPES 0 15 M NaCl 3 mM EDTA 0 005 Surfactant P 20 pH7 4 HBS P 10 mM HEPES 0 15 M NaC0 005 Surfactant P 20 pH7 2 HBS N 10 mM HEPES 0 15 M NaCl pH7 4 HBS N 10X 0 1M HEPES 1 5 M NaCl pH7 4 10 10 mM HEPES 0 15 M NaCl pH7 4 PBS 10X 0 1M phosphate buffer 27 mM KCI 1 37 M NaCIl 10 10 mM phosphate buffer 2 7 mM KCI 137 mM NaCI 5 DMSO pH7 4 Biacore 3000 1 2 3 Command Rack Base 1 lI My THEHMH_C Haaa JI JO JOY OOOOOOOOc OOS Fack Hase PFo c pea THEHHII_B THEHh4H LC OK
18. Sensor chip SA DNA Sensor chip NTA NTA His Tag Fusion Protein Ni Sensor chip HPA C18 monolayer a Sensor chip L1 bilayer Biac
19. Detection 1 3 _ r ml rr 1 r m 1 2 1 Fc1 2 3 4 r lmef rer we t Biacore 3000 4 41 OK ks kg 20 lt 50 ul min rm SS Crem
20. Enter Next gt 28 Next gt B LALTIRII JWA mr hm im mi TU HmmJEH FUFI eeTi rrP mOil WI NM 5 Ignore WM rm mh Fe Wii RTRJ Ci LE F WPL le Ilgnore Emrtirs HrihrhrraHinn eu bith Pi Fleay Erte i Bie Fir reectiont mw Rs 20 beliem Tas lrgections saki 0mH bc pH 1 9 ImwcienTmmw Pedip Neede Siubaewboml me Re egemerahor bm Hep tho iets Cmm Regeneration Method Dissociation in Buffer
21. lt If Then 5 3 2 35 Biacore 3000 76 5 5 3 1 1 Fc2 2 Fc3 3 Fc4 No 1 No 96 96 Fc1 Run RunApplication Wizard FT FLOW 20 1 Sm Trea Peer Hoa Caea Dctor EE QR Detection Fc1 2 3 4 Ref 2 1 3 1 4 1 OK Biacore 3000 5 77 FEED DETECT ET LE FLOW 20 Injection Mode INJECT Solution Vary
22. 41 Acetonitrile 100 Dimethylformamide DMF 80 DimethylsulphoxidetDMSO 100 Dioxane 0 Ethylenediamine 1M Ethanol 100 Methanol 100 3 Methylaminopropylamine 1M Tetrahydrofuran THF 100 Biacore 3000 104 9 Surface Prep 9 2 Surface Prep Surface Prep Surfqce Prep FCC _ AE Biacore 3000 42 9
23. Surface Prep Biacore 3000 125 oe 40 oe oe eo eo eo eo eo eo eo eo eo eo eo eo eo eo eo e oe eo eo eo eo e e oe oe e 417 19 21 2 33 oe oo oo eo eo eo eo eo eo eo eo eo eo eo ee eo eo ee eo eo ee 19 eee ee ee 17 84 84 oo oo eo oo eo eo eo eo eo eo oo oo oo oo eo eo eo eo eo eo eo ee eo ee 97 46 9 16 lt 33 ee 2 37 38 19 20 21
24. 60 4 84 IM Mass Transfef ee ee ee ee ee ee ee ee ee ee 1556 N Normalize eo eo eo eo eo eo eo ee ee ee ee ee ee ee eo oe ee es ee e oe NTA 464 LP Prime ee 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 6 94 96 97 QO OUICKINJECT RI Regeneration Scoutlmn9 ee eo oe ee ee ee eo ee ee ee eo ee ee ee 4 7 RInSe 96 9 SA 4 64 Sanitize 9 Specify Flow Rate qnd Injection Time se 2
25. f Then bound 20RU HHLT hme 1 ET mmmamm 1 Et EET EE 1ni mm td 1 EE RmCHWumDLT LN ll IF RelResp For Report Point bound In Flow Cell Subtracted Comparison ls less Than Value Constant 20 THEN Exit Cycle OK Biacore 3000 86 5 ETE bt Lr 1 EL iiee ET rtte 1 If Then regeneration 100RU
26. 6 Biacore 3000 8 1 6 Rack Base1 Rack Base2 4 7 mm 2 ml Thermo_A Thermo_A Thermo_C Thermo_C Thermo_A A D E F1 F2 Reag_A Reag_A F3 F7 Thermo_B R2 4 6 8 Rack Base1 Rock Base2 qa 1 3 R1A1 R1A2 R1A3 96
27. 2 Surface Prep 2 5 mm 2 Biacore 3000 9 Surface Prep 107 Po 1 2 0O 44 Oo Surface Prep Surface Prep 1 Surface Prep 2 O
28. Edit Customized Application 5 3 Biacore 3000 5 85 5 3 2 5 3 1 If Then 5 3 1
29. _ 20C Desorb Prime 1 2 Biacore 3000 7 97 Sanitize 1 IFC Sanitize BIA maintenance kit BlAdisinfectant solution 1 1 5 ml BlAdisinfectant solution BA 20 ml 2 15 ml 38 Sqnitize _ Saqnitize Prime 1 2 7 2
30. Immobilization pH Scouting Immobilization pH Scouting pH pH _ 5 200 ug ml 10 mM Immobilization pH Scouting 50 mM NaOH Biacore 3000
31. View Wizard Template Bs Edit Biacore 3000 3 27 3 1 2 Run RunApplication Wizard BIACORE 3000 Cnntrnl Software on COM1 File Edit wiew ommand i ools indow Helb p Run GBS FHFIT Es Run blication Wizard Riuh gthnn Data nllgctinn Hata sa Run Application Wizard Gtart halute ecowery Eiinding naluss Kinghic halusls Customized application se Template 1 ncgl Thg 5urface preparation hhlicahinn wizard gudes nnu Ihrnui h the essental steps In preparing the sehnsor surac Fnr malusls Imclumn Immnhlllzahinn pH sCnulih ligard imrmobilization reqerneratiohn scouting ard surface hlfnrrarice test Surface Preparation Start i Surface Preparation Surface Preparation EE 3 x utace Freparation Steps rmrmobilization pH Scouting rmrmmobilization Immnhillzahinn In Surface Frep Hgngneralin Scoutng utace Peformance esl udes nnu Ihrnu h the steps In Im
32. 1 20 kb 4 76 kb 1 20 kb 1 5 90 kb 5 5 150 kb Biacore 3000 9 Surfqce Prep 103 9 Surface Prep 9 1 Surface Prep Surface Prep Biacore 3000 UFC Surfqce Prep Surface Prep
33. Amine Cospiiag hpand Biacore 3000 32 3 14 2 Response 1 RU Response 2 RU NHS LT imire Cospling U igard EE Response 1 Response2 1 Ja Li i rr Response 2 Response 1 NHSs Response 2 Response 1 Biacore 3000 3 33 3 1 3 Immobilizadtion 3 1 2 urfaca Preparation Immnhilizatinn Frocedure
34. Sensor chip CM5 Research Grade 15 Certified Grade 5 Sensor chip CM4 Sensor chip CM5 Sensor chip CM3 Sensor chip CMV5 Sensor chip C1 Sensor chip CM3
35. S1 1 S1 2 PT er mm ait nra ph 1 mmm mmr Py FC me me es mml Regeneration Method Dissociation in Buffer Single Injection Two Injections Next gt 1 2 2 Biacore 3000 72 5 htmaos 3 i 1 1 an us Wa nl 1 a L LIN NINENEILILNL IL i EI 1 a g i t s a ma i WN ri HB 1 a P EF Bw f Ti LINHI II
36. Biacore 3000 122 9 Surface Prep i til rl pe Standby Flow After Run Prime Before Run Start ET Es Fe ner Eo Lr Dune msh hm Don t Save 11 EE a ismggi Mama tl Save Ctrl Break Run Log Biacore 3000 9 Surface Prep 123 Close
37. Evaluate Biacore 3000 100 7 BIACORE 3000 System Check hame Td Hr EE OD ri ET WT i 1 lrumrmmmiE TD Canin a Fum Ci Lm Tl FH ED rm TE 3 Fr In DBg RE ULT FF DL CH OH HH ar wl OL Ot In ii WT 1 LHF OH JHI ERILT HI Fe 1 4 nBTL WE HE IT HIP FT i FL 1 Ei FE FT 11 Ir LN RF check check Biacore 3000 8 101 8 Bia Users
38. Save Ctrl Break Regeneration Scouting Response brah bnred rn bat resem RU Tc ad i sm EG 1 _ Bm Regeneration Add Analyte Biacore 3000 50 4 Ea TH rm bs ct Tma mi Flow Ra lm pill Peten ia mh BS pl Tha de UMSGkgd UM Ju ptt GE 3 1 0 5 1 20 60 ul min ei Add
39. 1 100 unknown 1 2 Next gt Biacore 3000 5 93 I ama LE IE ra a Er Fr 3 OOOOKJgepoor 7 OS9998O JILJLJLJ Q82888O OC J oo oeeooos FC CC CR JJi J ITImWmn Cp ei be pe Next gt Standby Flow After Run Prime Before Run Start FNA Cre ytd Fe to Et EE dm ET Dont Save 11 Save
40. BSA 10 mM 10 mM Borate 1 M NaC pH 8 5 Biacore 3000 5 200 ug ml 10 mM pH 0 5 2 pH pH 3 5 mmobilization pH Scouting 10 mM pH SA 100 ng ml 10 mM Borate 1 M NaC pH 8 5 NHS
41. RelResp LT hae Lie rl EE Le Fa et Lui HE mp Il i EE mm I be I IGT TE EF Hm TPT MN FE et YF EF EN BPI HT et PF TI TT re i ET mm 2 1 3 File gt Save File Save As Tools Preferences Manual Run Automatic Save 15 Biacore 3000 16 2 2 2 File Print Print esulls Serreongrarn PE Fr Lktin Ever a 1 Graph Table Event Log All Cycles Fit to Page 1
42. pH _ Nac 10 mM Gly HCI HCI Phosphoric acid Formic acid 10 mM Gly NaOH NaOH Etnhanolamine Etnhanolamine HCI EDTA Surfactant P 20 Tween 20 Triton X 100 SD5S Octylglucoside Acetonitrile DMSO Ethyleneglycol in HBS buffer Ethanol Formamide GuQanldine HCI Urea Biacore 3000 4 39 18 Bulk Effect Bulk Effect 4 Bulk Effect Biacore 3000 40 4 4 1 Run Run sensorgram P i i EE I EE OK 19
43. FCO 1 Biacore 3000 9 Surface Prep 105 1 2 1 1 4 2 2 16 mm2 1 13
44. HLT EH ET0 MUTE md iNETET Gem iE 1 OCRLDTT LR EI GPCHTET mm ET em Cycle i mm Biacore 3000 5 75 30 ja i RS 36 lt gt r Kae iim itive om RM IFC
45. Binding Using Capturing Molecule Direct Binding Next gt Use Flow Cell s Flow Rate 20 l min Injection Time 2 3 min Dissociation Time 2 3 min Stabilization Time Run Order Random Sorted Low to High As Entered Analyte Name MW Repl Concentration 5 Biacore 3000 66 5 1 0
46. Draft Wizard Results OK 2 3 Ctrl Break Biacore 3000 3 17 3 90 CM5 Biqcore
47. Ff Biacore 3000 5 87 IF RelResp For Report Point regeneration In Flow Cell Sample Comparison ls Greater Than Value Constant 100 THEN Command Block OK END IF Next gt Biacore 3000 88 5 Standby Flow After Run Prime Before Run Start IP J Cre ju Flr td EE EE td lOLMIa swe swe psewl Dont Save 11
48. N g CM NHS N NHS S S S S Biacore 3000
49. Em ee LE WI 1 EE ram FT F 1 a EE i i EL PE Biacore 3000 5 91 5 3 Keyword Analyte OK Biacore 3000 92 5 PPT 1 1 Fi 1 malaksmsslakmsims Hs Repl 2 Analyte Standard1 1 Standqrd1 2 Conc ug m
50. Ctrl Break ii Fo Resp1 Resp2 Biacore 3000 74 5 5 3 Customized Application 1 If Then 5 3 2 Sequence 30
51. 43 2 2 UPCHURCH SCIENTIFIC MICRO FINGER TIGHT PEEK 10PK MICRO SLEEVE SLV 10PKI PEEK TUBING Naturall 010x 020x5 10 cm Polymer Capillary Tubing Cutter Biacore 3000 106 9 Surface Prep 9 3 9 3 1 Biacore 3000 Biacore 3000 Control Software Sensor Chip Maintenance hs Dock Prime a Command Rack Base 2 Surface Prep 9 3 2 Surface Prep SERVICE LOCK
52. R Coo CONHCH CH OH NH5 BR 1000 50 EDC N ethyLHN 3 dimethylqminopropyUcarbodiimide hydrochloride NHS N hydroxysuccinimide 1 M Ethanolamine hydrochloride pH 8 5 EDC NHS 10 ml 200 ul 7 mm 20C 1 4 C 200 ul 1
53. Q OiI IO I UTUOUOUOCUOCO th tule mm ta me Next gt 1 LE Et om Pr me DE EE EN EE Ef Nm ue se ET ed km mm Fee Re I tharos Pos dia Run Standby Flow After Run Prime Before Run Start Ta sm me se Ce Dont Sqve 11 Biacore 3000 3 35 E sa TL TE Ba EIJI ji Sqve Ctrl Break Response 1 2 14
54. A A A A A B B A seeeeeS9e FT 1 2 4 5 EO rh hn 1nh 110 Lntsinil Concentration Biacore 3000 90 5 Customized Application 5 3
55. Reag_A RR1 RR2 RR3 _ Biacore 3000 1 9 1 2 4 Command gt Set Temperature Em el mmgrluWr 4 40 C OK 7 4 40C Temperature
56. Sqave Ctrl Break 1 1 binding a ko 1 Ms kg 1 5 KA 1 M Kp M Rmax RU 1 1 binding _ Baseline Level Biacore 3000 5 69 5 2 Run gt Application Wizard a Hm ne ol mm WL af en mm aa eos i he Direct Binding Binding Using Capturing Molecule Direct Binding Next gt
57. 2 OK OK Delete Command i ET him i 1 Ra ED EOE bai Nm ET imi T NTT CE PUTWI ke am HH EE EE i mi MEN TT He PUTSW mmmasm Biacore 3000 8Z 5 33 0 1 Solution
58. Injection Time 7 min Flow 10 ul min Next gt 13 1 3 4 Blank NH5 i Surface Preparation Flow Rate and Injecktion Time_ mm mim _ EI Help lt Back gt gt Llngg Biacore 3000 30 3 a il hI EU Re i _ rE mi EF i km mattg re H ETC HC EN OC CO CC OD OO OOO i bi i ta a i Le sb lil mt TFP is or Fis RE Bm to ED
59. Run Run Application Wizard Surface Preparation Start Regeneration Scouting Next gt THIIIDE TB WP FL Ipsa Irwcten ima mm EkwRmr ko Im Fu eRe re Flovcet FE Ht Bmk Meas csm Name Injection Time 2 5 min Flow Rate 10 20 ul min Use Flowcell Next gt Biacore 3000 48 4 NII WI WI NN ME OL Es a s Eie Eta Bur EF pire Florer Am Fur E Standby Flow After Run Prime Before Run Start Dont Save 11 Biacore 3000 4 49
60. 40 4 2 1 kk 47 4 2 lL 52 4 3 kk 56 4 3 1 56 4 3 2 L444 444 60 Biacore 3000 TKT TE OO OO777Y77Y SKK 50000000omooneoeoeoeeoee 64 5 1 eoeoe eee 64 5 2 lk 69 5 3 ee 74 5 3 1 76 5 3 2 L444 85 5 4 kk 89 6 94 6 1 a 94 6 2 El 95 6 3 ki 95 96 7 1 96 7 2 kk 97 7 3 kk 98 4 EY cA 98 8 kk 101 SUC Pre EL eee 103 9 1 Surface Prep 103 9 2 Surface Prebp oocecGGGe ii 104 9 3 i 106 9 3 1
61. Biacore 2 1 EE 5 mm Biacore 3000 2 1 1 1 2 BIA programs BIACORE 3000 Control Software BIACGORE S000 Gantrnl 5aftmarm i LCOTE 0 Danirel Eula wn Ch Epiupe Ernaur a CE Fr mn Eu I er IFP Es Ep EEE re well PTIm D 1 ECH Td E 1 F pn pn a a hemi bar a 8 _ PT 1 a Rh mil rw JP Hensnrersam wr Im 1 L Im Il Ia 1 Il F I I 1 HI me Fa IE le are lm Smile ar TL 1 ajilnp 75 HS F Nl Pan hh RFIHF nt dl 7114 Apunp rH THANT I II TH Il HiFIl FaF hunk 1 lil 1 7 1 eq pin rr FI 1 Tn II TT PII kimh 1 mh 1 7 1 1 anlmp 0 G42 D1 rps ph nsFLHE ab_l wab 1 Repnriwmnt tle
62. Delete Up Down Biacore 3000 9 Surface Prep 111 GiQiIQICT DO OD nT apnoor ooo ci WiCI I ICU Nk ooo woo m Fm Next gt OO Standby Flow After Run Biacore 3000 Prime Prime Before Run Start Perrd se swe tenes Don t Save 11 Biacore 3000 112 9 Surface Prep lle i csm
63. Bulk Effect ng ml pg ml Ko 1 10 10 ug ml
64. Surface Prep Surface Prep 2 he 1 22 1 Surface Prep 2 Surface Prep 1
65. 10 After Id OK 3 DPPCHPET ETECTIOR BF FL aaF twa ET rkboky 1 i UECTEND EGFPONSE bors TUELT mM Hp HH ET EN ET ED FE SPONSE maermmaiom 5 Repl Antibody a Biacore ae 80 5 Antibody1 1 Antibody1 2 Run Order Random As Entered Step Next gt
66. Ctrl Break Biacore 3000 94 6 6 6 1 Standby 3 3 Standby Tools Start Standby 5 l min 96 96 Standby 80 ml Standby 2 Prime Tools Working Tools Prime Close 16 mm 3 ml R2F3 lt
67. Next gt Biacore 3000 118 9 Surface Prep Standby Flow After Run Prime Before Run Sstart ra pens rard Rn Lr Duart ead tres sm sm masm Exam Don t Save 11 Ga mi nie bl a Save Ctrl Break Biacore 3000 9 Surface Prep 119 Face Ty Er BE RA CN a
68. Biacore 3000 70 5 mmm et ismees Fb Im mm yet a Spi rrr TH mar mci Fe em mmmmiwrmmlit mmm Use Flow Cell s Flow Rate 10 20 ul min Number of Sample Injections 29 Injection Time 0 5 3 min Wait After Injection 2 5 min Next gt 29 Number of Sample Injections 4 3 2 Biacore 3000 Sample Repl Run Order Random As Entered SteD Next gt 5
69. TEL 06 6305 4707 FAX 06 6305 3599 TEL 03 5331 9336 Biqcore 3000 9 FAX 03 5331 9370 e mail Tech JP ge com 2010 GE GE 4 169 007 3
70. 4 Dock Biacore 3000 96 7 7 7 1 Tools gt Working Tools Prime Dock
71. 64 5 5 Customized Applicatlon 5 3 5 4 Kinetics Anqlysis Binding Analysis Customized Application Customized Application 5 1 Run gt Run Application Wizard aa Fh hn pH Wd Ths bets ui LL i rd dat Fos Ha Fa Hf mi cbr ng ratte Fa rai ay km A FE EE MP 5 FROPL cf Coat CFU ri pe re rr eki he ak i er kor cl mh tir cmmimrt hd cm a Emrfmurtrm wat Kinetic Analysis Start Concentration Series Next gt Biacore 3000 5 1 5 2 5 3 5 4 5 65 Direct Binding
72. Biacore 3000 5 4 Biacore A B B A RU A RU vs C 5 89 A A Slop A Slop vs C B A A
73. Save Ctrl Break 20 View Plot Single 1 Curve Sensorgrem Fea1 View PlotOverlay View PlotCurveClqsses Curves Biacore 3000 42 4 2 Command gt Inject INJECT 21 lrWei
74. IFC 6 30 Prime 2 3 Flush IFC 3 Rinse IFC 8 Desorb 1 IFC BIA maintenace kit BIAdesorb solution 1 0 5 SDS BIAdesorb solution 2 50 mM Gly NaqOH pH 9 5 22 2 Thermo_A R2F3 BIAdesorb solution 1 3 ml R2F4 BIAdesorb solution 2 3 ml 37 Desorb BIAdesorb solution 1 4C
75. Biacore 3000 108 9 Surface Prep 9 3 3 Surface Prep SURVICE LOCK lt lt UNDOCK SURVICE LOCK DOCK 9 3 4 Surface Prep BIACORE Surface Prep Surface Prep Biacore 3000 9 Surface Prep 109 9 4 Run Run Application Wizard 1 Sadooaoocia br ia Immobilization in
76. 3 25 1 ISO 9001 2000 TEL 03 5331 9336 FAX 03 5331 9370 e mall Tech JP qe com
77. 22 3 Run gt Run Application Wizard EF I LE ue Lr me sm Surface Preparation Start Immobilization pH Scouting Next gt pH Next gt Add Remove Biacore 3000 3 23 i i EE Fi gt ee LE mm Name Injection Time 1 min Flow Rate 10 ul min Use Flowcell Next gt 2 Wash Method Two Injections Solution 50 mM NaOH Injection Time
78. mm 1 Biacore 3000 26 3 iss ls pl ops si Es eg je a pm Le um pH 9 9 Immobilization pH Scouting pH pH NHS pH4 pH4 5 NHS pH8 5 pH4 pH pH pH4 5 View Wizard Results
79. 10 ee ee ee eo eo eo ee ee ee eo ee ee eo ee ee ee ee ee ee ee 98 3 ee ee eo ee eo eo eo eo eo eo ee eo eo eo eo eo eo eo eo eo ee eo eo eo ee ee ee 94 94 41 A ee 3 ee 95 Biacore 3000 126 ee 95 ee 37 ee eo eo eo eo ee ee ee eo oo ee ee ee ee ee eo ee ee 17 84 20 ooo 101 ce 46 ooo 1 oe oe ee ee ee ee eo ee ee ee ee ee ee ee ee ee ee eo ee e e 52 64 89 ee 15 5 6 94 96 97 cee ee ee ee ee 96 20 ce eee eee ee ee eee 96 7 8 ee 19 21
80. Biacore 3000 9 Surface Prep 115 9 5 1 ei mem Analyte Recovery Start i em em IFC MALDI MALDI MALDI 48 Next gt Biacore 3000 116 9 Surfqce Prep pa PR Fm Lil Per FF mm mri Ta mm ah 5 Fae srm wihEsamnt Fania Pe mh dh ser nH ammrrmm Ermtrramn rb Ta r ea Peeomiolnr aganmommEcatonams Feri h wah Re mh Lam He rBwh our me Injection Settings Flow Cell Flow Rate Sa
81. Response 22 Run Start New Cycle Ci Va Comard Kun Hoo Windom Hp Earsd a 2 Run gt Stop Sensorgram Biacore 3000 44 4 22 2 10 10 30 Apply to All Curves Binding of DSA 100ug ml to immo
82. m HE mm 1 ooo 1 aot ot HD OO 1 Ne joooeaot a CTooooon moo hr Fa ei a i Nh ot Next gt Standby Flow After Run Prime Before Run Start OL EE Em BTmE la sww mein pswel sre Don t Save 11 Save Ctrl Break Biacore 3000 5 81 31
83. eee ee 17 84 17 ooo 14 15 44 bY EO 2 TT 2 TT 2 eo 96 Biacore 3000 127 14 40 ee ee ee ee ee 98 Biacore 3000 128 INDEX A Aim for immobilization level yy ye ee ee ee 28 IB BIGINJECT e e e e e 2 Binding Analysis 469 C CIOG8e w eee no ev wowvewseeyvweeweeeseevbesvveesss 94 CM5 17 21 GOINUECW 4 ww WUovvo ovM MM 6 ku yo gt o42 Commaqnd OUueue 5 Customized ADplication ee ee eo eo eo eo eo eo eo eo eo eo eo eo eo eo ee eo ee e se 64 74 76 89 ID Desorb ee ee ee eo eo ee eo ee ee oe ee
84. Standby Flow After Run Standby 5 umin Prime Before Run Prime Start Biacore 3000 3 25 EAL EL Ll ean WA ty EE LE eae rd be T me Smmtr mtswe een 1 Don t Save 11 Save Hl Ctrl Break ri Fretl ll ratio pH Fin NF FE rn re I mi eta EE rr ys IFFT 1 FTiEmi ha W El bars ah FE rh er ti FE hc Elmer PH B lmamrobFH mm Tm em HH
85. pH 8 5 DMSO Instrument handbook Biacore 3000 3 1 1 pH CM5 CM CM 1 0 5 pH pH
86. View Reference Line Edit AddReport Point ma Add Hennrt Point Sehnsogram F Time 5 5 MinH Wi baseline 7 Baseline ly apply In Eurygs Id 0 RU Baseline RelResp 0 Biacore 3000 2 15
87. dd ee enerer lin ih EL rr Hd ewe Start Xa nd Regeneration Scoutind Regeneration Biacore 3000 4 51 Regeneration Scouting ResponSse a 3 3 DSAGDugern 1 10 mk Glycina pH 2n 1 mk Glycine pH 1 5 1 1 mh Glycine pH 1 5 1 HH Hgneheralinh Analyte 80 20 pH Analyte 80 20 20 80 2 90 Biacore 3000 4 4 2
88. mm EF EE ET ES E re a Lr CH Ee mn la Hu re EF Mass Transfer Next gt Bm rBek uy mem Direct Binding Binding Using Capturing Molecule Biacore 3000 4 57 Next gt PR Re CPRM gs WN He bet mem ems Use Flow Cell s Analyte Name MW Next gt Regeneration Method Dissociation in Buffer Single Injection 1 Two Injections 2 2 Solution Injection Time Next gt Biacore 3000 58 4 f i i 3 ED ml a Anio
89. 30s Next gt Biacore 3000 24 3 Lad 1 EEE TH Le Lm ee rm E m ETHIF LE II CK ICUIKJIK JCIOIOICIOI J DIOIC o 88888O COIOIOIPIGBIBCSJ BOODDSBBBO 8338983338e OU II rar ET FH a LE a El F ss F a MenuY Template Save As Next gt pd Fs Td de pH I ET Lr FF em Lr PP Fh bb EE 3 PH Fa m1 1 tr ata FF ker Flee Ld Ri ee et ew pm
90. Flow Cell Recovered Analyte RU 1 2 mmz 1000RU 1 ng mmz ng Standby Tools Stop Standby Biacore 3000 120 9 Surface Prep 9 5 2 Surface Prep Surface Prep 2 Run Run Application Wiza
91. Tools Working Tools Close Biacore 3000 6 95 6 2 Command Undock Undock Sensorchip Biacore 6 3 50 ml 4 C DNA 50 ml
92. My Computer Windows My Computer Bia C Bia Users lt File New Folder Enter Biacore 3000 102 8 Explore Start Program Windows Explores C Bia Users fFile New Folder Enter 39 Bia Users Biacore 40
93. Single Injection 1 Two Injections 2 2 Solution Injection Time Next gt Biacore 3000 5 67 oW OO OO OO Qi OIPI OOO OOO rT EU TT TET LT WT Next gt PT TUEEL EE EPCTI IE TLTTET pp TE Fi mi IP PIEL pi 5 PFILEIIL IE A PETT ILM LR Hg EE 1 FlLE lHHHE HI be lel bse wm ow Standby Flow After Run Prime Before Run Start LNA rn lo jr ng IO bt Ce weir emnetgla 7 Dont Suve 11 Biacore 3000 68 5
94. Save Ctrl Break 35 If Then For Report Point IF RelResp RU AbsResD RU Slope RU s In Flow cell RU Comparison ls Greater Than ls Greater Than or Equals ls Less Than ls Less Than or Equals EquQls THEN Exit Cycle Exit Method Command Block ff Then
95. 4 3 1 Mass Transfer SAN RTT NR rebinding 1 1 Langmuir with mass transfer Run gt Run Application Wizard Kinetic Analysis
96. 46 4 26 Commaqnd Stop Injiect pe ii SEE mT iri ere ey Ep ty 1 1 OK 27 More gt gt Predip Needle Position Extra Cleanup Biacore 3000 4 47 4 2 4 1
97. Save Ctrl Break Biacore 3000 4 55 Baseline Level Response Level Baseline Level Response Level Baseline Level Response Level Baseline Level Response Level Biacore 3000 6 4 4 3
98. 106 9 3 2 Surface Prep 4 106 9 33 SurfacePrebp io ee 108 9 3 4 Surface Prep zg lt kk 108 9 4 ee 109 5 22 EE E EE lee 113 9 5 1 115 9 5 2 Surface Prep 120 9 6 Surface Prep MM 124 kL 125 Biacore 3000 1 1 1 1 1 1 1 1 gt gt gt Windows biacore 30
99. Biacore 3000 2 1 1 2 2 3 4 5 Sucrose 2 SUC TOSG 4 SUC OSG 8 Sucrose 16 Sucrose 2 1 1 100 l Run Run sensorgram al Tv Tir r Frere a nn Fe
100. Immnhilzatinn FrnceHure Specify FlInwi Fiate and Inection Time Help Back bl Llngg Aim for Immobilized Level Next gt Hm lmh ts mm Ligand Name Target RU Wosh Solution 50 mM NaOH Blank 13 Next gt 15 I RU 16 NHS 5ul min 7 5ul min 5ul min 7 Biacore 3000 34 3 oOOOOO GO COO DO
101. Fr EE EE Lu NH Ju Detection mode OK Biacore 3000 12 2 Fm C NBia Users Save Command Inject 10ul Stqrt Injection Biacore 3000 2 13 Injection of several concentration of Sucrose Response mF re i FE LaF re ETE i 5 ca Run Stop Sensorgram Biacore 3000 14 2 2 1 2 RU
102. Standby Tools Stop Standby 48 MALDI MALDI MALDI Holder MALDI Holder MALDI Holder MALDI Holder MALDI Holder MALDI i HHn Larngl Brukar HH gl hd target bd Bl FD ample plate THU well HBEE UIH sample plate T1932 mml EE ABI 4700 sample plate 192 well cal MALD BI Wnnaner sarnnle late TOD well Hoder MALDI Hoder BI nuaner sample plate 192 well Bruker well MALDI target 1 a 7 Micrnmass dE well 4 re wl AL tarr Shimadzu Fratos HH well MALDI target MALDI Holder MALDI Waters Micromass 12well 2ref strip for Q TOF Ultima Global PerkinEImer MDS Sciex MALDIchip target 96 well PerkinEImer MDS Sciex MALDIchip tqrget 384 well Biacore 3000 124 9 Surface Prep 9 6 Surface Prep Surface Prep Wash 1 509
103. Biacore 3000 4 37 4 crude
104. 1 epip HS AL D1 uu AH pinr ub ub_ 1 4d FriL i i Fa Ii H bl Tinn Tin ET HF 1 TI rie pea ri 1 CUE EL EL FIirm IIIITmin Firm LT mE LC ral 1 4 nmpemhire ri ID Inrdnrlinn Find tinglin Fer hmn Esent lng Tahle eo SE i E Menu bar Biacore Toolbar Sensordgram window Report point table Eventlog window X 4A Status window RU Run Biacore 3000 1 3 1 2 1 2 1 Dock mH WO
105. Kp RU RU 200x1 Sx RU 1000 x 1 S x S 50 kDa 100 kDa 1 200 x1 1x 50 000 100 000 100 RU 1000 x 1 1 x 50 000 100 000 500 RU 100 500RU Biacore 3000 3 19 3 1 N g CM NHS N NHS
106. Wizard Templates a 7 gh Coupling Concertration Mskhm HPA Chip Coupling bl HPA 7 Ligand Tl Coupline bly 0 2 Manual Ex hibition assaybly ki Marual Ex2 Consecutive Regenerationbl i Marual Ex3 Positive Controlbly 5 7 Manial Ed Fositive Un eyery titth ctl 4 NTA Chip Cauplingbln NTA NTA Chip Preparing bl NTA 7 Frepating bly SA Surface Thiol Coupling tly Run gt Run Application Wizard Customized Application QOpen template Open Fe lehry cde Crain Wi TErrmnLmi TT i fray mu rain nhn tn Fa re rt ah cE iw ee i Tre ee mmm bf aa gtr rh lpr mnra a
107. ee ee eo eo ee ee ee ee OO Dock 3 E Extra Cleanup Ss eo eo ee DD ee ee TD ee ee ee ee ee ee ee ee ee e LF FLUSH 96 LH HPA 4 64 IThen 8588 Immobilization 7 Immobilization pH scouting lt 21 INJECT 11 42 Biacore 3000 129 LK Kinetics analysis 64 KINJECT 2 LL Linked Reaction
108. i Standby Flow After Run Prime Before Run Sstart Dont Save 11 Biacore 3000 4 63 Sqave Ctrl Break hb eam 1 im am 0 Corcengabon exes Baeine Level Mans Tuansler Lnked Roactont 1 1 1 1 1 1 binding Biacore 3000
109. lsopropanol a 109 6 0erie eid BIAdesorb solution 1 2 509 DMSO BIAdesorb solution 2 ES SDS BIAdesorb solution 1 1 2 Surfqce Prep Wash 1 Thermo A 2 Surface Prep R1F3 1 R1F4 2 1 20 2 10 49 Surface Prep Dock Prime Surface Prep Wash
110. 8 LU Uncloggind eo eo eo eo eo eo eo eo eo eo eo eo ee ee ee ee ee ee ee ee ee eeQJ Undock ee ee ee ee eo oe oe eo eo ee ee eo eo eo eo eo eo eo oe ee e ee e es O WI Wizard Template eee ee ee ee 4 Biacore 3000 H9 3 NN OU 6 DR 9 F OD HH
111. Biacore 3000 36 3 Aim for immobilized Level NHS Too fast binding of ligand pH Too slow binding of ligand pH
112. C NHS EDC NHS Next gt EE rt ET Fi bur ma Fe me EE pl Lp pn a re Eee EF eer Pr EE am le Standby Flow After Run Prime Before Run Start Saw sam pmwsms LEcscm Dont Save 11 Biacore 3000 rm SW 8 EE EE Ei 3 31 EE i ur a 1 a Fs Pl r mm mmr OO ce 5 Save Ctrl Break
113. ENILILIHIELHELILI mH Ll Fl i i i a Ei El i 8 EE i IL i 6 Fw Li 1 Next gt CEE LT i EE E i rit Wim I mm mm 1 al 4 1 31 5 Ph 1 FF LEE mm _ Standby Flow After Run Prime Before Run Start ww i i a BE Lr J 1 Dont Save 11 Biacore 3000 5 73 Suve
114. Form Save Ctrl Break DRE 15 re ELC grd TH 1 1RS 15 LL Tk dE ns ie snluhinn IFPaIIEB riashim hal rmeciiahi WW 1 1E1 EIDPIICNIEP4E lnclrm Limard 1 ELL Ed1 1B lnclf mmmkmmmia H1a5 ELL 5 wihnn after WeChinhi W We 1Eg WE tA Lr ras ed i tr OL 1 rc gr RI rm mmmmm Li Close Standby Tools Stop Standby Surface Prep Biacore 3000 9 Surfqce Prep 113 9 5 Surface Prep
115. GE Healthcare Biacore Biacore 3000 Ver 4 0 Instrurment Handbook GE imagination at work 1 LL 1 1 1 1 1 1 1 1 1 1 2 2 2 3 0 EEE EE EK 3 21 AA A ka 3 1 2 2 kl 5 123 YORE DGDpj A A 7 0 SC 9 1 2 5 SPR Ce 10 2 kk 11 2 1 kk 11 2 1 1 11 2 1 2 ae 14 2 1 3 LL dR R R 15 2 2 16 EE 16 3 I 17 3 1 I 19 3 1 1 pH Lk 21 3 1 2 27 3 1 3 33 4 37 4 1
116. Injection Two Injections Solution Injection Time Next gt 4 53 1 2 2 mm ht E hn_ 1 T 1 IE P 1 Next gt Biacore 3000 54 4 mew Ebote Hun ry Pt ifr Fae Standby Flow After Run Prime Before Run Start EauttUt CT Do sou ment tve amnt wad engla mW etn pemspe Dont Save 11
117. Surface Prep Next gt Surface Prep type 1 Surface Prep type 2 Next gt Biacore 3000 110 9 Surface Prep EE nl HP EE Fir TI LL Lin FPPFTP Hm tgek ma mmm 1 Next gt 45 Injection Sequence Edit rtm Nive im lrWri Pam RW mml Tim 1 Ete W in Me eect me ee Bew ace OK 5 300 ul 1 100 l min Add
118. bilized Asialofetuin 4 10000 T T fa Status window view Reference Line View BaseLine 0 Biacore 3000 4 45 23 Command Queue Command Queue
119. by Cycle Volume OK DETEENON 1141 rms Injection Mode QUICKINJECT Solution Vary by Cycle Volume OK Biacore 3000 78 5 rm NEE TCO 3 1 FUR 20 UERUEET Titd Chet pH a ET UNCERLEET EM HH HI THEHI EE 5 DETECT 1 141 FL a 10 Before Id Baseline OK 1 FJ DEIEmON LL LP 0 EL WET anahod TO RECT PND UIPHHNIECET mMGgHCnGF Biacore 3000 5 79
120. er 1 a b ee i Standby Flow After Run Prime Before Run Start EACH Dont Save Biacore 3000 4 59 Save Ctrl Break mass Tarnsfer_tes2 Resulls 0 9 130 150 180 HD 3a0 300 30m mr BaekmeiLytl Haan Tumaler Lnked feathon
121. ew a ei _ mm rBek jus mm Direct Binding Binding Using Capturing Molecule Next gt Biacore 3000 4 61 Use Flow Cell s Analyte Name MW Next gt Regeneration Method Dissociation in Buffer Single Injection Two Injections Solution Injection Time Next gt 1 2 2 Biacore 3000 62 4 r P n Eg _ mm i m i Nt LL FC NE NL NE NE NL NL ELD LRN RN
122. mnhllzinn ligard or capturing molecule on the sehsor chip surface Help Br Magt Llngg Immobilization Next gt Biacore 3000 28 3 a Surface Preparation Immobilization IX Sensor Chip rmrnobilization Method Amine Lnunlin Help Sensor Chip CM5 Immobilization Method Amine Coupling Next gt lt ack gwt Clnse mmobilization Fracedurg lim Fr Immobilized Lawl Help Hack IK hl Llasg Aim for Immobilized Level 3 1 3 Specify Flow Rate and Injection Time Specify Flow Rate and Injection Time Next gt 12 NHS 5 ul min 7 7 5 ul min 7 Biacore 3000 3 29 lt ack agl 5 LInsg Ligand Name
123. mple Injection Time Wash Settings Sensor Chip Wash Solution Flow System Wash Solution1 Flow System Wash Solution2 Recovery Settings Incubation Time Recovery Solution Add Reagent Recovered Analyte Reagent Solution Reagent Volume Wash Needle with Solution Next gt Biacore 3000 1 2 1 10 ul Add Reagent Recovered Analyte 9 Surface Prep 117 irtF RET res mE 3 5 0 LT1 3 114 EE 6 7 9 L20 Sample Name Repl Next gt CO On OF Qi I CO iP iPi i doo GO COI CPIO CY CTO Oo wm aa Bg ih omct tpt ae ae ae nn He ems tbk ety Com
124. ore 3000 1 5 1 2 2 Dock Working Tools Prime I Faas E 1 Start Prime Exit Biacore 3000 6 1 5 Prime Prime Tools gt Working Tools 0 22 um CM5 10X
125. rd ha mm Surface Prep unit In Via MALDI On MALDI target1 MALDI MALDI 48 Next gt Biacore 3000 9 Surface Prep 121 i Bl or pai 116 Next gt Sample Name Repl Next gt a 5 8 a a a a ki RL mml ys PL a LT m LC a ps Lg Lj Ol J 1 em fa F mf mm a a sm a a on rms arm LE 1 Next gt
126. rm in Fe 1 liihJgrT Pt P xaw ga rr Tar 1 EE inrriummmtmmt Detar mmr han Hitme ss Start Injection Re sponSse 21 Re QUICKINJECT 5 325Ll 10u COINJECT 2 Sample 1 0 100ul Sample 2 0 100ul BIGINJECT 325 750Ll 52Ll MANUAL 30Ll INJECT Biacore 3000 4 43 Command Inject Star Injection
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