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TaKaRa PCR Thermal Cycler DiceTM Operation Manual 取扱説明書

1. Delete User Name Tools Select Tools Delete User Delete User Data 1 MENU User Enter TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx User Common Tools User 05 05 05 12 00 00 25 0 C Select User Name OK Enter 2 Tools Enter Select Tools TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User Common 05 05 05 12 00 00 25 0 C 3 Delete User Data OK Enter Select Tools MENU MENU History Setting GC PC Communication Cancel Cancel Diagnosis Reserved Delete User Data TD3010JE_v3 0
2. 1 History OK Enter EES MENU User Common 10 Progrm TaKaRa Taq Type 1 Tube 50ul Started at Fail ed Recovered at 15 07 59 02 11 26 4 15 25 31 02 11 26 15 31 54 02 11 26 3 Power TD3010JE_v3 00D 38 TP600 TP650 History 4 3 Y MENU 2 History 4 NOTE 40
3. LINK 1 LINK 1 21 LINK 1 4 LINK 3Short LINK 4 History 2 2 lt ProgranCLINK 1 Mode 1 O ul Started at 11 50 10 05 05 21 Flnished at 15 28 15 05 05 21 2 Setting Setting 2 1 1 Setting OK Enter LET 2 Date amp Time OK Enter Date amp ITImelllll Default Setting TD3010JE_v3 00D 39 TP600 TP650 3
4. K k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k Kk
5. HHHHHHHHHHUHHHHHHHUHLHHHHL LILHLHHHHLHLHUHUHHHUHHHHUHHHHUHUHHHHHHUHHULHHLH HHHHHHHHL HHHHHHHHHUHHHHHHHHHHHHHHHLHHHHL TD3010JE_v3 00D eiillliilluiliuililullitliiulltuti L HHHHHHHHHHHHHHHHUHHHHHHHHHHUHL LILHHHHHHHHHHHHUHHHHHHHHUHH LILHHHHHHUHHHHUUHHHHHHUHHHHHHHHHHHHHHHL HHHHHHHHUUHHUHHHUHHHHHHUHHHHHHHHHHLHL HHHHHUHHUHHHHHHUHHHUHHHHHHHHHUHHHHHHHH LU D e lilii III I IU Ll HHHHHHHHHHHUHHHHHUHHHHHUHHHHHHHHHHL HHHUHHHHUHHHHH UUUUUUUUUUUUUU eiililllulllililul lil IMM UU WU UU OD CE EE EE BE HHHHUHHHHHHHIHHUHHUHHHHHHUHHHHHHHHHUHUHHHHHHHHHHHHL HHUHHHHUHUHHUHHHHHHHHHHHHHHLHHHHL HU HHHHHHL HH gooo EL eae tinte 000000000 000 fm W Disassembly Prohibited UU 000000000 Hu gt D montage Interdit HH HHHHHHHHH ul 000000000 Warning High Voltage HHHHHUHHHHHHL k Attention Haute tension EL EL E E BE E LI HHHHHHHHHHL HHHHHHHLH Sos 00000000000 Mise Terre Obligatoire 00000 mires 0000000 0 00000 0 0 0 0 HHHH aeree HHHHHHHHHL 0 00 OI High Temperatures Haute Temp rature BIBLE 0000 UE DT TH 000000 ee 3
6. e 0000 0000 00000 000 000 U IP 0000 0 0000 D 05 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHL IlHHHHHHHHLHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH OE Ee RE i py lai EE BID PEE E 000001 00000000100 00000000010000000000000 LL ELEDE EH BE BL EI EL EFLOE 0000 CLE CECI 00 BEER 00 0 00 000 LU 06 00000 00 00 00000 HU 00 0 0 000 EE JUD 0 00 L 0000 00 00 0 0 0 00 00000 0 U 0000 0000000000 0000000000000 Long 00 ELIO BEL EE UE BE BELL 7E 0 0 000000 PCR OL UO UO EL ELLO ELO DD Tempe 0 HHHHHHHHHHHHHHHHHHHHHHHHHL FIT p h ii iii ii HHHHHHHHHHHHL IIHHHLHHHHHHHHHHHPCRHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHLHHL HHHHHHHHUHHHHHHHHHHHHHHHHHH 0900 00 00001 e e HUUUDUUUUUUUUUUOO TD3010 JE_v3 00D TP600 TP650 0000 eil lll 000 000 HHHHHHHHHHHUHHHHHHHHHHHHHHHUHHHHHUHHUHHLH HHHHHHHHUHHHHLHHHHL HHHHHHUHHUHHHHHHHHHHHHHUHHHHHHUHHHHHHHHH HHHHUHHHHHHHUHHHHHHHHHHHHHUHHHHHHHHHHHHLH 100010 N 10 11 12 13 TD3010 E v3 00D HHUHHHHHUHHHHLHHHHL HHHHHHHHHHHHHHHHHHHHHLHUHHL HHHHHHHHHHHHHHHHHHHUHHHHUHHHHHHHHHL HHHHHHHHHHHHHHLHUHHL HHHUHHHHHHUHHHHHHHHHHHHHHHHHHHHHHHHL HHHHHHH
7. 94C30 G50 G60 5 72 1 Remaining 0 07 Grad 2 lt 3 Grad OK Gradient Temperature 01 49 8 02 50 04 51 8 05 53 07 55 4 08 56 10 58 9 11 59 Result of Gr a d Test Heat 3 1 Cool 2 0 C s OK Over 0 2 C UnderO 5 C Started at 16 21 42 03 01 15 Finised at 16 31 05 03 01 15 Length of Run 00 09 23 Resul t Nor mal 2 lt 3 1 C OK Enter TD3010JE_v3 00D 46 TP600 TP650 5 Delete User Data 1
8. Start Receive a program from pc ancel Receiving 4 PC gt PC 5 OK Enter 6 SaveAs Program TaKaRa Taq ance User Common penn Ger NOTE 4 5 3 TD3010JE_v3 00D 43 TP600 TP650 4 Diagnosis PCR 4 Rate Test Cycle Test 3 Up Down Test 4 C
9. Enter Confir nati on OK 9 Are you sure to delete Program Name TaKaRa Taq TD3010JE_v3 00D 48 TP600 TP650 Cancel Enter Delete User CK Enter Confirmation MENU Are you sure to delete OK User Name Ichiro NOTE Programs exist Delete All Program in User Are you sure to delete OK Cancel All Program in Common OK Enter Seek and Erasing 15 TD3010JE_v3 00D 49 TP600 TP650 IIHHHHHHHHHHUHHHHL 0000 000000000000000000000000000 History 0000000000 Resut See history HHHHHHHHHHHHIHHHHHHHHHHH See history 0 0 00 000 mH H H DHE HUB 0000 00 iti 0 0 000000000000 00000 000 00 0000 ooo Saa HHHHHHHHO ____
10. 2 2 gt gt gt RS232C 9 D sub 3 2 Dice 1 PC Communication Enter Select Tools MENU History Setting Sve PC Communication S vc Cancel Diagnosis Reserved Delete User Data TD3010JE_v3 00D 41 TP600 TP650 2 Senda program to PC OK Enter PC Communication MENU Send Program to pc Cancel Receive a Program from pc Send Enter Program Name Stored RT PCR 03 04 01 m Send Cycle Seg 03 04 01 TaKaRa Taq 05 04 01 Touch Down 03 04 01
11. pp EE LU DL D DE D 0 0000 00 B following repairs shall be subject to a fee even during the warranty period Malfunctions resulting from incorrect operating procedures customer repairs or renovations Repairs resulting from moving the system to a different location on site moving it to a new site dropping or transporting the system c Malfunction or damage resulting from an act of God including earthquakes or lightning pollution or abnormally high voltage input oa saog d In cases of failure to produce this warranty card e In cases where the customer has failed to fill in the date of purchase or has altered the information on the warranty card f In cases where the cause of the malfunction does not originate in the product itself and improvements are required g In cases where causes the malfunction originates in system supplies that need to be replaced 2 Takara Bio Inc shall not be responsible for malfunctions for accidents that occur when the product is connected and used with equipment not recommended by the company 3 Be sure to consult with Takara Bio Inc or the place of purchase prior to moving the product 4 Store this Warranty card in a safe location as it cannot be reissued UUUUUUUOUUUUUUUUUUUUUUOUUUUUUUUUUUUU
12. OK Enter LET SAS Y 2 M D Y 3 0 Y 03 01 15 14 15 00 2003 1 15 14 15 00 Date Style 1 Date 030115 Time 141500 OK Enter 2 2 1 Setting OK Enter 2 Default Setting OK Enter Default Setting Stand by Temperature LCD Back Color 1 LCD Sleep 1 1 Buzzer Key Pad 1 1 Buzzer Finished 1 1 3 OK Enter Stand by Temperature 25 LCD Back Color 1 2 Sleep 1 30 2 NOTE MENU Buzzer Key
13. 1 2 Buzzer Finished 1 2 3 6 TD3010JE_v3 00D 40 TP600 TP650 3 PC Communication 3 1 PC Windows 2000 XP 1 gt gt gt C Program Files Windows NT hypertrm exe 2 TaKaRa 9600 8 1 Xon Xoff ASCII gt gt ASCI ASCI
14. gt 0000000000 000 0 Gave UW 000 OU 00 00 EE 0 000 0 00 00 00000 00 gt 0000000000000 GaveAs 00000 000000 Save DO U OUEnterQ HI TD3010JE_v3 00D 27 TP600 TP650 Save Program MENU program SaveAs User Common UU UU UU UU ut ii 0 0060 00000 000 JU 000000 0000000000000 save 00 00 000 U UU D 0000 UU UU UU UU bittulbliiLiuliliuttLtLtL i Enter 00 00000 0000000000 Save 00 00 enter 000 0 0 00 0 000 00 0000 UU U UU UU 000 00 0 0 00000 000 00000 000 000 0000 00 0 0 SaveAs 00 0 00 D Enter 0 0 00000 0 000 000 00 NOTE 0 00000 0 SaveAs 00 0 Enter 0 Program Narre 0000000 0000000000000 000 00 0000000000000 D D UU D 3aKaRa Save SaveAs 000 0 0000000 HHHHHHHHHHHHUserlHLHHHHHHHHHHHHHHHHHHH UU 00000 0 00 00 000 0 000000000 00000 000 00000 HHHHHHHHH O 00000 0 lt gt aKaRast 010 0000 OO 00000 000 0 _ 0 00 00 0 0 0 000 0 0 0 00000 0 6 HHHHHHHLHHHHHHHHHLHHHHHHHHIHHHHHHHL U U HHHHHHLH 1 000000000000000000000 Userr Enter OU TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx User Common User 05 04 20 12 00 00 25 0 C TD3010JE_v3 00D 28
15. Progrm TaKaRa Displays events 1 Tube 50 15 07 59 02 11 26 Showing 3 of the 15 25 31 02 11 26 past 4 events 15 31 54 02 11 26 Started at Failed Recovered at Power The number of recorded events is shown to the right of History on the top line The example shown on screen indicates that information is displayed for 3 of the past 4 events and does not indicate page count More events can be viewed by pressing the Down arrow key Maximum recorded events 40 TD3010JE v3 00D 96 TP600 TP650 Operation Manual lt NOTE gt To display the history of continuation the pop up menu is erased and Push down arrow key or pushing the MENU key twice When the denominator of the fraction on the History side is four or more on the history screen the history is displayed on the following screen Please do not forget and look lt NOTE gt History display when link function is used example LINK 1 LINK 2Long LINK 8Short LINK 4 The history record is displayed as LINK 4 4 when running from LINK 1 to LINK 4 History 2 2 lt Mode 1 TTT Dul Started at 11 50 10 05 05 21 Finished at 15 28 15 05 05 21 The start time of the first programis displayed as start time The finish time of the last programis displayed as a finish 2 Settings Function Time Date and System Default The Settings function includes time date syst
16. 4 Gradation Test TP650 25C 1 Diagnosis OK Enter Setting PC Communication Diagnosis Reserved Cancel Delete User Data 2 OK Enter Self Diagnosis Rate Test Cycle Test Cancel Up Down Test Grad Test NOTE Result Enter THE 3 Start Enter 20 23 TD3010JE_v3 00D 44 TP600 TP650 D RateTest 25C30 94 C30 40 C30 Remaining 0 02 Start
17. m e AN KA ANA Mt SAN ANN A A A Y A e 4 OOOOO OOOO RS232C DIUBgagab utl HHHHUHHL HHHHHHUHHHHHHHHHHLHHHHL I UW UU UU D EIE ELCE EI 000 000 000 000 D 0000 00 00 0 0000 HHHHHHHHHHL RS232CHH 000 00 0 0000 000 000 0 0 0 00 00 00 00000 UNOTERS232C JT OU BB D 00000 TD3010JE_v3 00D TP600 TP650 OOOO MENU 000 0000 00000 6 POWER I T pm 0000000 ONU Hg D UBI 00000 0 00 00 0000 Lu uultu t TaKaRa PCR Thermal Ope STOP I CE 000000000 0000000000 00000000 0 00000 00000 0000 0000000000 0000000000 TD3010JE_v3 00D TP600 TP650 0100 000 00 EL 00000000 012600 000 650 000000000000 TP650 0 0000000000000000 0000000 00000000000 0000000000 00400 0000 00000000 000000000000 00000 0 0 0 0 O MENU 0 0 00 0 00 000 00 000000000 0000000000 00 000 0 0000 0 nn 000000000000000000000 MENU 0 00000 0 0 0000 000000000000000 0000000000000 nr D 000000000 HHHICEHHHHHHHHHHHHHHHHHHH 00000000000 00000000000 00000000000 0000 400000000000000 00000 0 0000000000 400000000 UU SOUUUUUUU 00 WU 000 0 t Uu L UU gu 000000 00 000 0 H UUDUILL 00 0 00 00000000 00 000 000
18. Dice 1 Choose PC Communication go to pop up menu select OK and press ENTER Select Tools History MENU Setting Diagnosis Reserved Delete User Data 2 Choose Receive a Program from PC go to the pop up menu select OK and press ENTER PC Communication MENU Send a Program to pc Receive a Program from pc 3 This displays the Receive a Program screen Go to pop up menu select Start and press ENTER Confirm that the Receiving message appears Progress MENU Start Receive a program from pc Receiving 4 On the PC use HyperTerminal Go to Transfer choose Send Text File and select the send file name Send the program from the PC to Dice lt NOTE gt For the correct PC transmission settings refer to PC Communication Settings in section 3 1 Transferring a Program to a Computer 5 Information for the received program is displayed when the process is completed 1Temp 3Temp 25Cyc Time 10 00 Go to pop up menu select OK and press ENTER TD3010JE v3 00D 100 TP600 TP650 Operation Manual 6 Save program under new file name MENU SaveAs Program TaKaRa Taq User Cancel User Common lt NOTE gt For a description of the file saving procedure refer to 3 Saving New Programs in section 5 of Chapter 4 4 Diagnosis Function Running Operation Testing Programs This function performs self diagnosis tests These tests are
19. Cancel Result of Rate Test MENU Heat 3 1 C s Cool 2 1 C s Over 0 1 C UnderO 4 Started at 15 47 21 03 01 15 Finised at 15 50 52 03 01 15 Length of Run 00 03 31 Resul t Nor mal onan Heat Rate gt 0 Cool Rate gt 2 0 2 Cycle Test 94C30 55 C30 72 30 Remaining 0 02 Statt Cancel Result of Cycle Test Heat 3 0 C s Cool 2 1 C s OK Over 0 2 C 4 Started at 15 39 09 03 01 15 Finised at 15 42 39 03 01 15 Length of Run 00 03 30 Resul t Nor mal lt 0 5C lt 0 5C lt 4 TD3010JE_v3 00D 45 TP600 TP650 3 Up Down Test 98 1 4 1 Up Down Test Remaining 0 02 Result of Up Down Test MENU Heat 3 2 5 Cool 1 4 Over 0 2 C 3 Started at 15 59 23 03 01 15 Finised at 16 05 49 03 01 15 Length of Run 00 06 26 Resul t Nor mal lt 10 Gradient TP650
20. 7 Transient over voltages according to Installation Categories Over voltage Categories For mains supply the minimum and normal category is II 8 Pollution degree 2 in accordance with IEC 60664 3 2 Installation Use the following procedures when installing system 1 Confirm that the system power switch is set to off 2 Connect the system side of the power cable to the power connector on the rear of the system 3 Plug the AC power plug of the cable into a power outlet The system is designed to operate normally with a 100 to 240 V AC 50 60Hz power line Make sure that the power outlet is designed for a 3 pronged plug with ground line In the interests of safety and avoiding electric shocks be sure to CAUTION connect the system to a properly grounded power outlet 4 Turn on the system power 5 The following input prompt screen appears as soon as power is applied Select User Name Common TaKaRa Cancel 3 Moving the System Follow the procedures described below when moving the system to a new location after it has been installed e New Location Within the Same Site Follow the precautions listed below when moving the system to a new location within the same site 1 Thoroughly clean the system and make sure that there are no tubes or plates remaining in the sample block 2 Avoid any shock during movement that can affect the operation of this highly sophisticated system 3 When lifting an
21. TP600 TP650 2 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHL 00000000000 OKU Enter 1L Select User Name lt Common gt lt TaKaRa gt MENU Jiro Sabro OK HHHHHHHHHUHHHHUHUHHHHHHHL IIHHHHHHH lt gt O U UU 000060 LEL EL OL EL EL D DEDE DE CE HHHHHHHHHH 0000 3 HHHHHHHHHHHHHHHHHHHHHHHHHHH MENU User Jiro HHHHHH 000 00 0000 0000000 0000000000000000000000000 4 000000000 00 9 00000000 00 0 00 U U HHHHHHHUHH 1 000000000000000000000 User Enter 00 TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx User Common User 05 05 05 12 00 00 25 0 C 2 0000000000 New Enter 00 Select User Name lt gt New TD3010JE_v3 00D 29 TP600 TP650 3 HHHHHHHHHHEnterl HHHHHHHHHHHHHHHH 0 SaveAs Enter IMENU_ MENU Save As User 4 0000000000000 00000 0000000000 LHHHHHHHHHHHHHHHHHHHHHHHHHHHH HH OK Enter 0 00 HHHHHHHHHHHHH 000 HH 0 HHHHHLHHHHHHHHH HL a ji U U HHHHHHHUHH 1 000000000000000000000 Usern Enter 00 TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx User Common User 05 05 05 12 00 00 25 0 2 HHHHHHHHHHHHHHHHHHHHHHHHHHH OKH Enter 00 Common TaKaRa Jiro UUUUUUUUUUUUUUUU 000
22. Dice reaction time Reaction volume 50 Ll Row D Reaction Mix O PCR Conditions 1 x PCR Buffer 94 C 30 seconds 65 C 10 min at 30 cycles 200 dNTP mixture 10 H M each primer Row G 1 ng A 1 25 U TaKaRa Taq Cat Row H up to 50 ul All 96 wells were used for the Dice TP600 reaction Random samples were placed 3 per row for the SP reaction After the PCR reaction 1 Agarose gel electrophoresis was performed using 5 H of reaction mixture lt Result gt Dice TP600 samples amplified nicely in all wells with hardly any inconsistencies indicating reactivity equal to or better than the SP TP400 TD3010JE_v3 00D 116 TP600 TP650 Operation Manual Application 4 Comparison of 35 kbp DNA Region Amplification for TaKaRa PCR Thermal Cycler Dice vs SP e Evaluation target Compare differences in 35 kbp A DNA region amplification between TaKaRa PCR Thermal Cycler Dice TP600 and TaKaRa PCR Thermal Cycler SP TaKaRa LA Taq Cat RROO2A was used for the reaction Reaction tube TaKaRa Micro PCR Tube Cat 9047 e Reaction mode 2 Selected at TaKaRa PCR Thermal Cycler Dice MODE 2 e Reaction volume 50 0 with addition of 25 pg template 50 e PCH conditions 94 C 1 min 98 C 10 s 68 C 15 min 30 cycles 72 C 10 min After reaction 1 Agarose gel electrophoresis was performed using 5 H of reaction mixture TD3010JE_v3 00D 117 TP600 TP650 Op
23. Tahara TaKaRa PCR Thermal Cycler Dice Version Ill Model TP600 TP650 Gradient Standard Operation Manual TaKaRa PCR Therrmal Cycler Dice 0 00 000 0 000 0 0 0 0 0 00 000 0 00000 HHHHHHHHHHHHHLHHHL HHHHHHHHLHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHLHHHL Attention Users are recommended to carefully read the contents of this manual before operating the TaKaRa PCR Thermal Cycler Dice system Carefully observe all special Warnings and Cautions outlined in this manual This manual should be stored for reference in a secure and accessible location 233J 2 C ARSL TAKARA BIO INC NOTICE lt Only for US gt Purchase of this instrument conveys a limited non transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under one or more of U S Patents No 5 038 852 5 656 493 5 333 675 5 475 610 and 6 703 236 claims 1 6 only or corresponding claims in their non U S counterparts owned by Applera Corporation Noright is conveyed expressly by implication or by estoppel under any other patent claim including under U S Patent No 6 814 934 which claims thermal cycler apparatus and systems capable of real time detection Further Information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA Applied Biosys
24. Taq Antibody PrimeSTAR HS DNA Polymerase PrimeSTAR GXL DNA Polymerase RO45A PrimeSTAR MAX DNA Polyrrerase PrmeSTAR 000000000000000000000000 http www takara bio co jp I 00000 TD3010JE_v 3 00D TP600 650 00000 e 000 0000000000000 HHHHHHHHHHHHL 0000000 TEL 03 3371 8553 FAX 0 3271 7282 TEL 077 543 7297 FAX 077 543 7293 0000 TaKaRa PCR Thermal Cycler Dice 00000 TPODO lI No HOO 000 I u aaa ss uli Eo HHHHHHHHHHUHHHHHHHUHHHUHHHHUHHUHHHHUHHHHHHHHL LE A E E E EN E EMEA E OO EN a E 00 00 00 00 0 00 0 00 0 0 0000 9999999999999999999999999999999999999999999999999 D 0 0 LI C IN UUW 0000 0000000 000 00080 0000000000 0 00000 OUUW UO 000100 090000 0000000600 OHHHHHHHHHHHHHHUHHHHUHHHH OHHHHHHHUHHHHHHHUHHHHHHHUHHHHHHHHL C 999999999999999999999999999999999999999999999999 HHHHHHHHHHHUUHHHHHHUHHHHHHHHHHHHHHL 00 0 0 00 0 0 0 0 00 aL ly UU TD3010JE_v3 00D 58 TP600 TP650 Operation Manual Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Appendix 1 Appendix 2 Appendix 3 TD3010JE_v3 0
25. 04 49 0 05 51 06 53 07 56 2 08 58 09 61 10 63 0 11 64 5 Uggagopuutgpeurmauutututuatututuacanadautt 0 HHHHUUHHHH HHHHLHHHTHHLHHHHHHHHHHHHUHHHHHHHL 0000 O 000076 0000 0000 EE EE Ge E EE 0002 Qd 000000 00000 000 0000 HHHHUHUHHHHHHUHHHHHL 00000000000 Enterr D E HHHHHHUHHHHHHHHH 000000 Ex GHHHHHHHHHHHHH 00000000000 Savel Enter D L i1Tempi3Temp 25cyc 2Te MENU Time 10 00 0 30 0 30 0 00 0 0 00 TD3010JE_v3 00D 21 TP600 TP650 e HHHHHHHH IIHHHHHHHHHHH Terrp EX 0 00 Slope Enter 000000000 i3Tempi 1 x MENU 1 RE C E RSE ER i Time 10 00 60 00 15 00 Ex 0 00 0 00 0 00 0000 O app 00000696 000 O 000000000000000 010 000000 O 0 00000 L HHHHHHHHHHHHUHUHUHHUUHHUHHUHHHHHHHHHHH HHHHHHHHHULHHHHUHHHHHHHHHHHHHH INOTEH HHHHHHHHHHHHHHHHHHHHHHHH 0000000 0 MENU Slope Enter 00 D 00000 00 0 0 0 0 00 O 0000 000 0 00000 00 0 L 00 0 000 Save Program as Program See User Common 0000000000000 SaveAs Enter 0000000 0000 00 00 000 0 000000000 000000 0000 0000 0000000000 0000
26. UD GP EN61000 6 1 2001 EN61000 6 1 1 2001 EN61010 1 2001 TD3010JE_v3 00D 54 TP600 TP650 eil HHHHHHHHHUHHHH 0 0 gd 00000 000 00000 Ut 0 00 0000000000 0 00000 0 0000000000600 0000 TaKaRa 0 PCRHHHH 000 00 0 271 Hi Tube Dome Cap 00000000 00000 200 1007 0 211 8 Tube 00 0000 00000 300 120 0 2m 8 Dome Cap DO OOOO DDD 301 12 TD3010JE_v3 00D 55 TP600 TP650 ei Et EE SEL LED BE UTE D LI E ooooo 3 3000 02 0100000000 Nan __ MED 0 2m Hi Tube Flat Recovery nmnnnnonon 0 2m Hi 8 Flat Cap 10 E 0000 TD3010JE_v 3 00D 56 TP600 TP650 HHHHHHLPCRHHHHHH nu nuu j U __ TaKaRa Ex Tag RRO1AM RRO1BM 4 TaKaRa Mg free Buffer RROLCM AMx 12 TaKaRa Ex Tad Hot Start Version TaKaRa LA Taf RRO2AG TaKaRa LA Tad with GC Buffer RRO2BG TaKaRa LA Tad Hot Start Version TaKaRa Tag 12 TaKaRa Tag Hot Start Version SpeedS TAR HS DNA Polymerase RRO OA RRO OB RROOSA aa Premi Tad Ex Tag Version 500u Ix 60 1200 0506 ES TaKaRa Versior 500u Ix 60 0 120 0504 IPCRH RROO5A PerfectShot Ex 7ad Loading dye mix 25 H Ix 48
27. 000 000000 HHHHHHHHHHHHUHHHHHHHH EnterQO 00000 DHD BEBE 000 000 0 3 User 000 000000000000 000000 000 000 0000 00 0 0 User 00 0 enter DT TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx User User 05 05 05 12 00 00 25 0 C TD3010JE_v3 00D 30 TP600 TP650 4 UserlHHHHHHUHHHHHHHHHHHHHHHHHHH ut 1000000000000 Edit Enter 00 lt gt TaKaRa Jiro Sabro NOTE 0 0 0 00 0 0 00 0 0000 0000 0 Sane User T 0 000 00000000 00 00006 o 000 0 0000 000 0000 000 Enter O O BE B 00 00000 0000 C OK Enter User Sakura Password HHHHHHHUHHHHHHHHL HHHHHHHHHHHHH 000 00 HHHHHL HHHHHHHHHHHHHUHHUHHHUHHHHHHUHHHHHHHHHL 7 000000000 00 00 0 0 00 00 00000 0 0 01 TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx user akura X User 05 01 01 12 00 00 25 0 C TD3010JE_v3 00D 31 TP600 TP650 NOTE HHHHHH 1 HHHUHHHHHHHHHHL OT 3 5 QRS 7 UVt uv a WX Y 2 WX z 9 L3 L3 L3 L3 L3 L3 L3 L3 P3 L3 L3 L3 m HHHHHHUHHHHHHHHHHH NOTE 0000 SaKaRalHHHUHHHHHHHHHHHHHHHHHH ilu 000 0 00 00 0 00 0 00 0 6 000 0 0 1 1Kbp lt 5Kbp lt 10 210Kbp 0000 00 0 000 0 0000 000000 PCROUUOUUU 00 0000000 0 D TeKeRa C 000 OO D 0000
28. 0000000 000000 00000000000 Grad EnterQ 00 0 00 000 000 00 0 0 0 0 0000 Gradient MENU1 OK 02 46 0 03 47 0 04 49 0 05 51 4 06 53 8 07 56 2 08 58 6 09 61 0 10 63 0 11 64 0 65 0 0000000 00 00 000 0 0 0 08410 0000 00 000 000 0000 0 0 00 0 60 0 0 0000 Hl 0000000 0000 O 000076 O C MUU QE UO D D UD 7O DO EE CI CE 000 0000 HHHHHHHHHHHHHHUHHHHH 00000000000 Enter U HHHHUHHHHHHUUHH 000000 ExT T utut D 00000000000 Save Enter 00 0 TD3010JE_v3 00D 26 TP600 TP650 00000000 0 00 00 000000 Terp EX 0 D J 0 Slope Enter 000000000 3Temp 1cyc MENU 1 0 0 0 0 Slope Time 10 00 60 00 15 00 Ex 0 00 0 00 0 00 HHHHHHUHHHLH 00006 O UBI e ampi 0090 0000696 OOO O 000000000000000 010 000000 HHUHHHHHUHHHULHHLHHHHHHHHHHHHHL HHHHHHHHHHHHHHHHHHHHHHUHUHHHHHHH 0000 00 00 00 00 0 000 0 0 00 0000 0 UNOTE 000 000 000000000000 0 00 0 01 MENU Slope Enter 0 00 0 00000 00 0 0 0 0 DU 00000 O 0006 00 000 00000 00000000000 UW UU UU OU UU 00 0 00 0 000 00 00 0 0 00 gt HHHHHHHHHHHH UW UU UU UUUU
29. 01 01 12 00 00 25 0 2 Select first program Example PLANT _TEST PLANT TESTAZ PLANT PLANT TESTAZ Programs numbered 1 to 4 will be executed in that order Select PLANT TEST4H go to OK and press ENTER 3 Go to pop up menu select Start and press ENTERI to display Select Reaction Mode screen Mode 1 1 3 Container 1 1 Thin 2 Thick Volume Link 4 Enter desired reaction settings confirm 1 is selected for Link field line 4 then choose OK in MENU and press ENTER 5 Next go to MENU select Start and press to start execution of program PLANT TESTZ Execution of PLANT TESTZ2 begins automatically as PLANT 1 completes Execution continues with PLANT TESTZ and PLANT TEST and terminates TD3010JE v3 00 93 TP600 TP650 Operation Manual Note Execution can skip the beginning programs and start with PLANT_TEST 2 for example and continue through PLANT TEST 3 and PLANT_TEST 4 Notel lf a gap occurs in the order execution terminates with the last program before the gap Example Execution that begins with TEST 1 terminates with TEST 2 Start TEST Sto TESTU TEST Executing Programs Intended for Link Function Individually Programs created specifically for Link execution can also be executed separately 1 Select program name go to MENU select Start and Press ENTER to display the Select Reaction Mode screen 2 Enter 2 for Link then selec
30. 10 28 32 05 01 20 stopped Stopped at 11 47 12 05 01 20 The second time of power recovered When the electric current is cut off two or more times and others have an error it indicates by a history at the order of time A maximum of 40 events Attention If a program is newly performed History at the time of the past operation will be eliminated Note Please refer to the page 107 of an Operation manual about Events and Error Code Table TD3010JE v3 00D 109 TP600 TP650 Operation Manual Chapter 7 Specifications Product name TaKaRa PCR Thermal Cycler Dice Model No TP600 Gradient model TP650 Standard model Operating environment Ambient temperature 15 to 30 C Humidity 20 to 80 RH Temperature accuracy 0 5 C 30 0 C to 99 9 C 2 0 C 4 0 C to 15 0 C Temperature uniformity 0 5 30 0 C to 99 9 Temperature setting range 4 0 C to 99 9 C 1 second to 99minites 59seconds Input 9999 Display entry panel unit angle 4 positions from perpendicular to 70 C Tubes and plates 0 2 mL X 96 tubes 96 well micro plate 12 X 8 rows PCR reaction volume setting 5 micro liter to 150 micro liter Program capacity User names max 22 excluding and Number of programs max 200 Lid temperature Approx 110 C for the 20 0 C to 99 9 C range Approx 40 C for the 4 0 C to 19 9 C range Added functions Gradient
31. 10 s and 68 15 min at 30 cycles 4 Preset Protocols with Slope function See next page Mutagenesis 94 C 10min 94 C 37 C 60min and 37 C 15min lt 1 Kop siRNA double 95 C 1min 70 C 1min 70 C 37 C 60min and 37 Differential DD 94 C 30s 40 C 1min 40 C 72 C 2min and 72 C 30s RNA 30 C 1 min 30 C 65 C 15min and 65 C 20min TD3010JE_v3 00 90 TP600 TP650 Operation Manual Mutagenesis 94 C 10min 94 C 37 C 60min and 37 C 15min Mutagenesis siRNA double 9590 1min 709C 1min 70 37 C 60min and 379C oo siRNA double 00 0 00 T Differential DD 94 C 30s 40 C 1min 40 C 72 C 2min and 72 C 30s Differential DD 4Temp 40 BcaBEST RNA 302C 1 min 30 C 652C 15min 652C 20min BcaBEST RNA TD3010JE_v3 00 91 TP600 TP650 Operation Manual 8 Link Function The Link function is used to execute multiple programs consecutively The Cycler Dice is capable of inputting a maximum of 12 segments with one program Users wishing to execute even larger programs have the option of running multiple programs consecutively Program names are assigned the symbol followed by a single digit number The Link function executes programs consecutively in the order of the assigned numbers The Cycler Dice is not optimally suited to operating in a modular configuration in which the required modules are linked together Creati
32. Cancel 4 PC gt gt 5 Start Enter Start Cancel Send a program to pc Program Name lt TaKaRa Taq Finished Finished 6 Cancel Enter 3 CE 3 3 3 PC gt Dice 1 Tools Enter PC Communication OK Enter Select Tools History Setting PC Communication Diagnosis Reserved Delete User Data TD3010JE_v3 00D 42 TP600 TP650 2 Receive a Program from PC OK Enter PC Communication MENU Cancel Receive a Program from pc Send a Program to pc Start Enter Receiving
33. OOO L 0000000000000 00000000000000000 0 amp 0 HOO 0 C a b 2 D E F 3 G H I g h 4 010 J K L j k l 5 M N O m n o 6 P 5 q r s 7 V t u v 8 W X Y Z W x z 9 TD3010JE_v3 00D TP600 TP650 OO Ill 0 IACIIIILIVIUI 000 D UU 00 0000 0000 0 00000 000 0000 0 00 0 0 D 0 0000 UUOUUUOUUUUUUUUU UU 000 00 0 0 00000 00000000000000 HHHHHHHHHHUHHHHUHHHHHHHHHHL HHHHHHHHHHHHHHHHHHHHHHHHHHHHH OKU EnterQ 00 lt TaKaRa gt Sabro Cancel NOTEH 0000000000 000000000000000 m0 0000 000000000000000000 000000000000000 00000000000000000 000 0 0 0 0 00 0 000 00 0 0 0 0 0 00 uu Common TaKaRa 0 000000000 0000 00 000000 D 00000 0 000 0000 00 User Jiro Password dac NOTE 0000000000000000000000000000 DOU uiulilii B 0 00 0 0 0 00 0000 0 000 HL 00 000 NOTE 000 000 0 000000 00000 0 000 000MENUG p Cancel DU D D Enter 0 00 0 D 000 000 00000 00 0 0 0000 00000 TD3010JE_v3 00D TP600 TP650 OO 00000000 00 EnterlHHHHHHHHHHHHHHHHHL 000 00 00 0000 000 00 000000 TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx RUN User Commo
34. Paused at poo 4 Remia ON 6 Recoveredat HHHH 8 000000 00 0 9 0000000000 0000000000000000000000 10000000 0000 TD3010JE_v3 00D 50 TP600 TP650 0000010000000 0000000000000 0000000 0000000000 0000006 00000000 000000 0000000000000000000 0000000000 00000000000 000000000 00000 000 1000000000000 00000000 0 0000 0000 000 0000 00000 00 HUUDUDUUUDUDUDUuD ul 000000 00000 0 0 0 000 00000 00000000 LU BUD B 0000000000 LH p Bali HHHHHHHHHHHHHHHHL E E 0000000000 00781 00 00000000000 000000 0000000000000 HHHHHUHHHL HHHHHHHHHHHL 00000 HHHHHUHHHHL 0000000000 00781 00 00 0000000000000 0000 Hui 000 00 000 HHHHHHHHHL HHHHHHHHHHHL HHHHHHHHL HHHHHHHHHHHHHHSeelHHHHHHHHHHHHHHHHHHHH 000000000000000000 operation manua 0 0 0 D 0000000 0000000000600 000000000000000000 0000000000 0 000000 000 0000 0 Default Setting 000000000 u 000000000000000000 000000000 0000000 039 00000000 0 00000 0 00000 00000000 000 000 D D U D D DL I Default Setting LCD Sleep No0 I II 00000000 Default Setting LDC Sleep 0 000 00 DU 000 0000000000 pp 000 Lu B 0000000000 TD3010JE_v3 00D 51 TP600 TP650 9 p o T hi i lila LCDH 1 D D D DE D D 0000
35. at 14 33 41 03 01 15 Length of Run 00 19 18 Over 0 0 C 3 C Result St opped Explanation of content of display Status Program Name User Name Program beginning time hour minute second and the date Program end time hour minute second and the date Operation time hour minute second The maximum overshoot and maximum undershoot program s operating Result Going to the pop up menu and selecting OK again displays the main program screen TD3010JE v3 00D 13 TP600 TP650 Operation Manual 5 Creating New Program New programs are created by going to the top screen selecting and editing a basic program pattern format then assigning it aname When creating a new program from an existing user file or simply editing the file refer to the instructions provided in section 7 User Selection and Registration 1 From the main program screen go to the pop up menu select New and press ENTER TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User Common 05 04 20 12 00 00 25 0 C 2 This displays the New Program screen Programs are created by editing segments Temp count and numerical values 1 segrert pattern 3 segments pattern Definitions of Terms Segment Represents the smallest unit which is equivalent to 1 Temp unit Pattern Represents a combination of segments for which numerical values are continuously set Procedure for setting Values Use ar
36. block Temperature setting range 40 0 to 75 0 C Temperature difference 6 0 to 20 0 C Notel Exceeding the temperature settings or the temperature setting range may destabilize controlled temperature The temperature values for 02 to 11 are calculated values Temperatures cannot be automatically incremented decremented for each step Go to pop up menu select OK and press ENTER When displaying the temperature gradient the left temperature is shown on the Temp line and the right temperature beginning with G on the Temp Ex line Go to pop up menu select Save and press ENTER 3 Saving an Editing Program There are two procedures for saving an existing program 1 Saving the edited program under the existing name Save 1 2 Saving the edited program under a new program name Save 2 3 Saving the edited program under a new program name SaveAs 3 Go to pop up menu select Save SaveAs and press ENTER TD3010JE v3 00D 84 TP600 TP650 Operation Manual Save Program V Save male Program SaveAs SaveAs User Cancel User Common 1 Press under the existing name Or select Save from the pop up menu and press ENTERI Overwrite Save 2 Press ENTER under the new name Or select Save from the pop up menu and press ENTER Saved but the former program is erased 3 Press ENTER under the new name Or select SaveAs from the pop up menu and press ENTER T
37. for programs 6 Temp size is 200 Program name character Maximum 16 Max number of registered users 22 excluding pre registered Common and lt TaKaRa gt usernames Notel New programs cannot be registered to the TaKaRa folder as the Save and SaveAs functions are disabled blanked out as in the pop up menu Select User in the pop up menu and save the program under a different username Save Program MENU Program User TaKaRa TD3010JE_v3 00D 19 TP600 TP650 Operation Manual 6 Editing and Saving Existing Programs Existing programs can be saved under a new name newly created program or under the same name edited program Go to the top screen select Edit and press ENTER TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User 05 04 20 12 00 00 25 0 C This displays list of programs registered to the User Choose the program to be edited select Edit in the pop up menu and press ENTERI TaKaRa Taq Cycle Seq Touch Down PCR 05 04 01 Cancel This displays the existing program and enables editing of the segment Temp count and numerical values Use the arrow keys to move the cursor Press or an arrow key after setting temperature Temp cycle count Cyc and numerical values Temperature setting 4 0 to 99 9 C Time settings 00 01 1 second to 99 59 99minutes 59seconds 99 99 when operating in continuous Cycle count maximum 99 Bloc
38. function only TP600 History function Diagnosis function PC communications RS 232 PC transmission software not provided 40 0 C to 75 0 temp difference 6 0 to 20 0 Safety devices Power switch with 10A over current breaker Lid Heater temperature fuse Thermal guard for reaction block heater plate 260 W x 345 D x 260 H mm 11 5 kg Conformity standard CAN CSA C22 2 No 10101 1 92 No 1010 1B 97 LR108182 UL Std No 61010A 1 EN61000 6 3 1 2001 EN61000 6 1 1 2001 EN61010 1 2001 lt CE The unit with CE mark complies with EC directives 89 336 EEC 93 68 EEC and 73 23 EEC TP600 model S N D 6051 and over TP650 model S N E 1301 and over model TP600 model S N D 6051 and over TP650 model S N E 1301 and over D 6051 and over TP650 model S N E 1301 and over DIRECTIVE 2002 95 EC the restriction of the use of certain hazardous substances in electrical and electronic equipment Performance specifications apply when system is operated in the 20 C to 23 C temperature range Please request exchange of a lithium battery from a service engineer TD3010JE_V3 00 110 TP600TP650 Operation Manual Appendix 1 Confirm that the following items are included in the system package when delivered Accessories list and packing Standard Accessories Quantity 1 Power cable 1 2 Operation Manual booklet 1 3 Warranty sheet Included Operation manual 1 4 0 2 ml Hi Tube Dome NJ200 100
39. press ENTER TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User Common 05 05 05 12 00 00 25 0 C 2 Select the user name to be changed go to pop up menu select OK and press ENTER Select User Name MENU Common lt TaKaRa gt OK diro BILLIG When the password is set the password is demanded Enter password when the password entry screen appears and return to the top screen 3 Confirm the user name you desired Go to pop up menu select User and press ENTER TaKaRa Thermal Cycler Dice TP600 Ver 3 xx 05 05 05 12 00 00 25 0 C 4 confirm the user name that wants to change reverses by the User selection screen Go to pop up menu select Edit and press ENTER Select User Name Commons lt TaKaRa gt MENU Jiro T Edit Cancel 5 Enter a new use name Go to pop up menu select Save and press ENTER ave Cancel TD3010JE_v3 00 88 TP600 TP650 Operation Manual 6 Enter password when the password entry screen appears Select OK on the pop up menu press ENTER and return to the top screen User Sakura Password characters and length numbers 0 to 9 1 to 6 digits This displays the entered numbers on the screen Use the CE key to clear password If numbers are mistyped use the CE key to clear and re enter password To avoid registering a password simply leave the field blank select OK from the pop up menu and press ENTER To confirm the User name was ch
40. product for detailed description of the Warranty conditions and period This product is designed to ultimate safety standards Nevertheless should any safety related issues arise users are requested to immediately contact the nearest sales agent Precautions safety and accident prevention for System Use Warnings The various messages and labels provided on the product itself and in the various manuals are designed to ensure the system is operated safely to prevent personal injury or damage to property Users are strongly recommended to read and observe the warnings included with the symbols shown below handling procedures that can lead to serious Warnings indicate incorrect system operation and WARNING accidents Cautions indicate incorrect system operation and CAUTION handling procedures that can lead to accidents TD3010JE v3 00D L General Precautions Regarding Takara Scientific Instruments It is important to observe the various precautions described below to ensure optimum system performance and prevent accidents before they happen Ensure safe and correct system operation by carefully reading the precautions Precautions for Installation Location Observe the following precautions in order to prevent fires and electrical shocks Do not install the system on an unstable or sloped foundation or any other location subject unstable conditions f the system tips over or falls immediat
41. shall be presented as a statement of the safety of the equipment to be serviced by customers requesting servicing and maintenance work We request your cooperation as it is designed to ensure the safety of all those who come into contact with the system Note that we may decline to perform servicing and maintenance work if we do not receive the Safety Confirmation Form TD3010JE_v3 00D 119 Tahaha Iu D J Warranty Card TaKaRa PCR Thermal Cycler Dice Model TP600 TP650 DOOD BB DD 000 DD HHHHHHUHHHHHHHHHHHUHHUHHHHHHHHHHHHUHHHHH UU Thank you for purchasing the TaKaRa PCR Thermal Cycler Dice Model TP600 TP650 In case of malfunctions that occur despite correct operation on the part of the customer Takara Bio Inc shall repair the system in accordance with the conditions listed in this Warranty Customers may have their device repaired by presenting this Warranty to a Takara Bio Inc sales agent or at a Takara Bio Inc service location within the period listed below nog Warranty Period 1 year TaKaRa PCR Thermal Cycler Dice Model TP600 TP650 Serial Number UD Date of installed BERE JU Address amp Postal code Name of Company Institute Customer details Department Name TEL Personal z 2 L3 r3r3 adia r3 r3 J o ESI r3 a
42. used to check whether the system is operating normally when errors occur in PCR reactions The Diagnosis function performs the following four tests 1 Rate Test Tests heating and cooling rates 2 Cycle Test Tests cycle times and stability of control operations 3 Up Down Test Measures time required to cool down to 4 4 Gradient Test model TP600 only Measures gradient temperatures Confirm that standby temperature is set at 25 C prior to commencing tests 1 Choose Diagnosis go to pop up menu select OK and press ENTER Select Tools Hi story Setting v PC Communication Caneel Diagnosis Reserved Delete User Data 2 This displays a list of self diagnosis tests Choose the desired test item then go to pop up menu select OK and press ENTER Self Diagnosis Rate Test Cycle Test Cancel Up Down Test Grad Test TD3010JE_v3 00D 101 TP600 TP650 Operation Manual lt NOTE gt Choosing the target test item then selecting Result from the pop up menu and pressing the ENTER Displays the last results for each test item 3 When the diagnosis test screen appears select Start from pop up menu and press ENTER Test results are displayed when the tests are completed Reference values are for ambient temperature in the 20 to 23 C range 1 Rate Test program execute screen Program details 25 for 30 seconds 94 C for 30s and 40 for 30 seconds Rem
43. 0 0 00 00000 0000 L 1 1Kbpg 1Kbp 94 C 30sec 55 C 30sec 72 C 1min 30cycles gt q U De 2 lt 5KDD 5Kbp 94 30sec 55 C 30sec 72 C 5min 30cycles 2 eo 0 lt 1O0Kb 10Kbp O 98 C 10sec 68 10min 30cycles gt qT 4 gt 10Kbp J10Kbp O O 98 C 10sec 68 15mn 30cycles gt O eo I TD3010JE_v3 00D 32 TP600 TP650 5 0010000 000 Up ep umucumememu iD eu D BcaBEST RNA 65 0 Time 1 00 15 00 00 0 007 6 DIC Iu OD CD uu eu1wuu OD COE Differential DD 4Temp 40 1 881 0 00 7 Mutagenesis 00 C O00 iic Mutagenesis 8 HHLLHHHMML DUC OO be pi 000 IILI C OOU UD e siRNA double TD3010JE v3 00D 33 TP600 TP650 OO 00 00 0000 00000 0000000000000 000 0 0000 0 0 0000 0 000 00 0 000 L 0 0000 00 UUW 00000 00 0 0000 00 00000000 0000 0000 0000 0 00 00 0 0 000 0 00000 000 0 0 0 00 00000 000 0000 TE HH ET DEED ED E EE PLANT TEST PLANT TEST HHHHH 0 00 0 0000 PLANT TEST PLANT TEST NOTE 000000 0000000 10 D U CD D U U UI U HHUHHHUHHHUH
44. 0 0000 000 0 lt gt 0 TD3010JE_v3 00D 22 TP600 TP650 Note 0000 00 000 00 00 0 000 00 0 0 0 00000 00000000000 Save 0 00 100 00000 0000 LIBI EI 000 00 UE DELE 0000 6 sae MENU MENU Program Bier User TaKaRa OO 00000 00 00 _ 00000 00 000 00 0 0 8 0 0 CBE D 0 60 ELE C O U WUU OU UU UUUUU 00000 0000 0000 L 0000000000 000 00000000000 EdtH Enter 00 TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User 05 04 20 12 00 00 25 0 C HHHHHHHHHHHHHHHHHUHHHHENIERIHHHHHHHHHHHL 0000 0 D Edt Enter D Program Name Stored MENU TaKaRa Taq 05 04 01 11 30g m mn Cycle Seq 05 04 01 11 31 View Touch Down PCR 05 04 01 Wee User TD3010JE_v3 00D 23 TP600 TP650 0000000000 000000 0 000 0 00 0 0001 HHHHHHHHHUHHHHHHHLL 0 0 000 0 you 00000 0 00000000 O O L HHHHHH 0000 Buc ULULO O 0001001100 000 L 00000 00 Dee ET E U gab 0000 IIHHHHHHHHHHHHHHHHH OMA T5 00 T 03 Temp T T94 0 O30 ITI 00 00 00000 0000000000000 More 0 0000 0 rem 25Cyc l O 0 055 9 0 30 72 0 0 3010 0 00000 0000000000 D 0 000 00 0000 1000000 Insert 00000000000000000000000
45. 0 900000 00000 0 00 00000 00000 0 0 00 0 0 0 000 00 0000000000 00 00 000 Bg guum ut utu 000 0000 00 0 DMENU 00 0 0 00 00000000 0 B B LET BO 0 00 00 00 E Du 0000000 0000 00 00000 00 0 0 New Enter 00 TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User 05 04 20 12 00 00 25 0 C O0 000000000000000000 00 BUTemer 200 0 00 0 000 0 00 0000 0000 00 000000000 4 11000000000 Save UOUUU HUD 000 ETE 0 060 0000 0060 0 0 0000 00000 000 0000 00 0000 UU UU 00 00000 00 EVE HHHHHHHHUHHHHHHHL 0 Cepil O imeHH 000 0 Cyc HHHHHH Enter HHHHHHHHHHLH IIHHHHHHHHHHHHHHHHHHHHHHHHHLLIHHHHL 0000 0000 HHUHHHHHHHHUHHHHUHHUHHHHHHHUHHHJHHHHH HHHHHUH iip 004006008 OOO O 00 0000 0 BB 00 0 00000 0 0 0 L TD3010JE_v3 00D TP600 TP650 00000 0 0000 OO 000 0 0000000000000000 HITemp 00949 5 00 Tema D 10 3010 00 HB D D 00 0000000000000 Delete 0 000 00000 0 0 0 0 0000 Save 0 00 00 0 3 rem 25CydrTI 55 0 0 301172 0 0 30 TT 0 00000 00 00000 00 0 Hreert 000000000 U 0 0 0000 27 Bun Save Delete d bae lt More un lt gt Time 10 00 0 30 p gguuguuaiutiu 0 0 000 00 0000 tbt
46. 000 0 utt 00 00 0 00 00 00 0 0 0 0 0 0000 00 0 00 HH 1000 00 0 0 0 00 0000 Temp OL 0 00000000 BUP E D D HHHHUHHHUHHHHUHHHHHHH H HHHHHHUHHExtensiorHH HHHHHHHHHHHHHHHHHH Extension 0 0 0000 000000000000000 0 0000000000000 0000000000000 O 00 TD3010JE_v3 00D 24 TP600 TP650 HHHHH Pause DD HHHHHHHHHHHHTime ExHHHHHHHHHHHHHH HHPauseH Enter 0 ITP650 0 0000 000 00000000000000000 HHGrad Enter 00 000000 00000000000 0 0 0 000 OMEN Siope 00 D HH 0 0000 o 0000000000 00000000000 Morel Enter 00 1 3Temp 25 2 Te MENU 1 Pause Cance 0000000 0 Time OO 080 0000000000000 Enter 0 000 00000000000 0000 0 00 00 OEnter E O L Ex0 0007 6 OD OOO 000 00000 000 0 00000 0 000 Pause 00000000 0 Pause 0O BO D B D U U UOCE IL 00000000000000000000 Time nm ga 000 0000 LH BEBO 0000 00 UU 0 Enter 00 LEO ELE BEL OE EL BE UTE CE BOE BICI 000 0 0000 HHHHHHHHHLHHHHHHHHHHHHHHHHHHHHL Pause O O O OO O Pause ENTER 1 1 OD TD3010JE_v3 00D 25 TP600 TP650 INOTEI 00000 TemnlTime 0 000 00 0000000000 Insert Delete Gradient 7 000000 00000 Temp Ex 0 000 00 0 0 Slepet 00000000 Time Ex 000 00 0 0 PauseQ 00000000 0000000000 6500 0000000000000
47. 000 0000000000 OKO 0000 0000 000 History 00 00 0 0 0 0 0 000 0000 HHHHHHHUHHHHHHUHUHHHHHHHHHUHH TD3010JE_v3 00D 3 TP600 TP650 5 Tools 1 History 2 Setting 3 PC Communication 4 Diagnosis 5 Delete User Data Tools Enter TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User Common 05 05 05 12 00 00 25 0 C OK Enter Select Tools MENU OK Setting eve PC Communication Diagnosis Reserved Delete User Data Select Tools ASRVIV yA 4 a 6 OK Enter 1 History History
48. 00000 0000000 ii 0000 000000 i mm 0000 00000000 OOOO uuu 00090000000000 HHHHHHHHHHHHL 0000000000000 30400 I2 3 0000000 0000000000 100000000 HHHHHHHHHHL 0000000000 iu HHHHUHHHHUHHHHHHL 0000000001000 0000 0000 00 00 0 0 00000 0000 000 0 00 0 0 00000 000000 TEL 03 3271 8553 0 3271 7282 00000 TEL 077 543 7297 FAX 077 543 7293 0000 0 00 0 00 0 0 0 00 0 00 0 0000 0 0 0 0 000 00 00 00 0 00 0 0 0 00000900 0 0 00 0 00 HHHHHHHHL HHHHHHHHUHHHUHHHHHHHHHHHHUHHHHHHHHHHHHL HHHHHHHHHHHHHHL OOO OOOO C Finished ABC Test User Common Started at 09 47 12 05 01 20 Finished at 09 54 32 05 01 20 Length of Run 00 07 20 Power failed DO 0 00 0 0 0 00 0000 0000 Norrral HU 00000 Stopped 0 0000000000000 TD3010JE_v3 00D 52 TP600 TP650 000 D D 0 History ss jas User test Mode 1 Thin 50 ul Started at 09 47 12 05 01 20 Power Failed 09 50 34 05 01 20 UUUUUUUUO Recovered at 09 50 37 05 01 20 U DIDI mimm 000000000 sa 10 25 10 05 01 20 000000000 Recovered at 10 28 32 05 01 20 Stopped at 11 47 12 05 01 20 HHHHHHHHHHUHHUHHHHHHUHHHUHHHUHHHHHHUHHHH IHHHHHHUHHHHHHHL HHHHHHHHHHUHHHHHHHHHHHHHHUUHHUHHHHHHHH O OOO 000 UNOTE 0000 0
49. 000000 NOTE 0 0 0 000 00 B 0 7E 00 00 00000000000 0000 000 00000 UD D D UTI EPEANT 0000 0 00 NOTE DH DH 0 DD D HEU 0 0 000 ECL OE 0 0000 0000 00000 0000 000 000 5 00 0 0000000 TEST TEST TEST TEST 4000 0 0000 00 00 00 000 00001 0 000 00 E 000 0 0 00 000000000 1 00000000 0 Start Enter 00 Select Reaction Mode 0 1 0 2 Link 00 00 J Enter Mode 1 1 5 Container 1 1 Thin 2 Thick Volume ul 5 150 ul Link 2 1 Yes 2 No Link 02 No LU OU O EL OOL 3 MENU Start Enter 0000 0000000000 00 0 0 00 TD3010JE_v3 00D 36 TP600 TP650 NOTED 00060 000 0 0 0 0 00000 UW 00000 HHHUHHHHHHHHUHHUHHHHHHHHUHHHHHL Remaining time 00 D D D 00000 0 0 0 0 000 00 0000 Info 009000 000000000000 000 0 0 0 0 00 0000 DH E Finished Fnshedt 0000000000000000000 HHHHHHHHUHHHHHHHHHHHHUUHHHHUHHUHHHHHHHL HH History 0000000 000000 0000000000000 000000000 000000 LINK LINKAEJE 06000 0000 0 0 0 0 U U LINK Long LINK 0000000 LINK Short Program LINK 1 4 Mode Started at 11 50 10 05 01 21 Finlshed at 15 28 15 05 01 21 NOTE OO 0 0 ang pa d 000080000 00 000 000 0000 00 0 Teos Enter 0000000 History 0 0
50. 00000000000000 OK Mode 1 1 5 Container 1 1 Thin 2 Thick Volume 50ul 5 1501 Link 1 1 Yes 2 No HU UO COMO O 00 000 MOULU 0000 0000 90090000000 0000000001 10000 0 00000000000 00 0000 000 00000 rip B 0 0000 0 Mode 0 0000000000 0000 0 00000 0 e h 111 EBD ER ELE HHHHHHHHHHHHHHHHHHHHHL 00000 0 0 0 0 Mop 00 000 0 00 000 000 TaKaRa PCR Therrral Cycler SPO 00000000 HHHHHHHHHHHHHHHL HHHHHHHHHHHHHHHHHHHHHHHHHHH TaKaRa PCR Thermal Cycler MP 0 9 00000000 DO WU UUUUUUUUUU 00 DOLO 00 0000 00 0000 0 0000 LI uuu TaKaRa PCR Therrral Cycler PP Mode 1110 0000 60 00 000 E 0 0 000 0 Container QOThick 00000000000000000 000 00 t 00000 OU 0 000 00 LI 000000 Micro PCR Tube 00 00 0 904700000 0 2m Hi Thbe Dome Cap 00 000 NJ 200 0 00 a 000 0000 TD3010JE v3 00D TP600 TP650 LVolume 5 15090 000000 00000 O 100 200000000 OO 0 0000 00 0 000 0 00 0 0000 00 0 0 0 0 000 0 0 0000000000000 UO 000000000000 OKOD O 0 00 00 00 00 6 00000 0 00 00 0 0 00 000 HHHHHHHHHUHHHHHUHHHHHHHHHHHL OHHHHHHHHHHHH TaKaRa Taq Remaining 1 27 U D 00 I D 00000 NOTE III I UO 0000 00 0 000 000 00000 00000 HHHHHHHHHHH UNOTE HHHHHHHHUHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHUHHHHL 00 000000000000 Sta
51. 0D TABLE OF CONTENTS Precautions for Using TaKaRa Scientific Instruments Table of Contents Overview Installation Requirements amp Maintenance 1 Installation Conditions 2 Installation 3 Moving the System 4 Maintenance Names of Parts and Their Functions System Operation Introduction Turning on System Power Starting a System Operation Program Terminating or Aborting Operation Creating New Program Editing and Saving Existing Program User Selection and Registration poe at up IUE exe pose cq ums Link Function Diagnosis Functions and Other Tools 1 History Function History of Events 2 Settings Function Time Date and System Default 3 PC Communications Function Program Send and Receive 4 Diagnosis Function Running Operation Testing Programs 5 Delete User Data Function Troubleshooting Specifications Accessories list and packing List of Supplies PCR Enzymes Application 1 4 Servicing Request and Safety Check 99 page 59 60 61 61 62 62 63 64 67 67 68 69 73 74 80 86 92 96 96 97 98 101 105 107 110 111 112 113 119 TP600 TP650 Operation Manual Chapter 1 Overview TaKaRa PCR Thermal Cycler Dice TaKaRa Code TP600 Gradient model TP650 Standard model Thank you for purchasing the TaKaRa PCR Thermal Cycler Dice system Until now conducting polymerase chain reactions PCRs involved repeating experiments several times in order to achieve optimum reaction condition
52. 0D 47 TP600 TP650 4 Delete User Data Delete User Data MENU MENU Delete User Program Delete User Name Delete All Program in User Delete User Data Delete User Program 1 Delete User Name Programs exist 3 Delete All Program in User Delete User Name 5 Delete User Program 4 Delete Enter Program Name Stored MENU A My App6789012456 03 04 01 Cycle Seg 03 04 01 TaKkaRa Taq 19051404 017 Touch Down PCR 03 04 01
53. 5 0 2 ml Hi 8 Tube 300 12 6 0 2 ml Hi 8 Dome 301 12 Operation manual Tube set TaKaRa Supplied PCR tubes Option Cus Podwimme se _ TD3010JE_v3 00D 111 TP600TP650 Operation Manual List of Supplies PCR Enzymes Cat Product Name TaKaRa Ex Tag RRO1AM RRO1BM AMx 4 TaKaRa Ex Mg free Buffer RRO1CM AMx 12 TaKaRa Ex Tad Hot Start Version RROO2A DE TaKaRa LA Tag Mg free Buffer 125U RROO2M BEEN TaKaRa LA Tag Mg plus Buffer 250 U RRO2AG TaKaRa LA Tag with GC Buffer 125U TaKaRa LA Tag Hot Start Version TaKaRa Tag Hot Start Version SpeedSTAR HS DNA Polymerase RRO7OA RRO7OB RROO3A Premi Taj VersiorrT00u Ix 61 EN ROMA Prerrix Tag TaKaRa Tag Version TB500u Ix RROOSA PerfectShot Ex Tad Loading dye nix 9002A PrimeSTAR HS DNA Polyrrerase PrimeSTAR HS Prerrix For more detailed information refer to the Takara Bio general catalog or check for online catalog at our website http www takara bio com or http www takara bio co jp TD3010JE v3 00D 112 TP600 TP650 Operation Manual Appendix 2 Application 1 Gradient PCR Using the TaKaRa PCR Thermal Cycler Dice LTP600 System 8 kbp amplification performed in 2 or 3 steps using A DNA template Reaction TaKaRa Tag 5 units u 0 25 H 10x Ex Taq buffer 5 H dNTP Mixture 2 5 mM each 4 Ll Primer 1 20 u M 0 5 ull Pr
54. 925 0055 00000 0000 2457 D IMM UU DE BI DE DE L a HEURE SSH 00000 00000 0 000 00 00 00 00000 0 OE OE UU uiu uuu Lu gut HHHHHHHHHHUHUHHHHHHHHL TD3010JE_v3 00D UU UU 0000000000 TaKaRa 00000 00 0 0 00 00 000 00000 0 000 0 00 0 0 000 00 00 00000 00000 0 0 0000 0000 0 0000 0 HHHHHHHHHH 00000 0000 0 0000 00 000 0 00 0000 L HHIHHHHHHHHHHHHHUHHUHHHHHUHHHHHLHHHHL HHHUHHHHUHHHUHHHHHUHHHUHUHHHUHHHHHHHHHHH 00000 0 0 0000 00 0000 0 00 0000 000 00 00000 HHHHHHHUHHHUHHHHHUHHHHHHHHHHHHHUHHHH 00 HHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHUHHHUHHHHLHHHHL HHHUHHHHHHHHHHUHHHHLHHHHL eiillbll liuuilliiuiililillililiilluiltLiu il tL HHHHUHHHHHUHHHHHHUHHHHHHHHHUHH e lilii UU L HHHHHHHHUHHHUHHUHHHHHHHHHHUHHHHUHHHHHHHHHLHHHHL eiilljli l lllllllillilliillllHilliL l 0000000000 COOU 000000 0 0000000000 000 UD Up L Urn pul 0 0 000 0 00 000 0 0 OL HHHHHUHHHHHHHHHHHHUHHHHHHHHHUHH TD3010JE_v3 00D LILHHHHHHHHHHHUHUHHHHHUHHHHUHHHHHHHHHLHHH HHHHHHHHHHHHHHUHHHHHHHHHHHUHHUHHHHHH HHHHHHUHHUHHHHHHHHUHHHHHHHHHHHHHHH LU 00 00000 LILHHHHHUHUHHHHHHHHHHHHHHHHHHUHHHHHHL 0 0000 00 0 0 000 0 00 0 0 0 0 00 0000 DL
55. Eo 0 0000 0 0 00000 OOOO UD ul OOO 00 DO 0000000000000000000000000 HHHHHHHHHUUUHUHHHUHHHHUHHHHHHHHHHHHHHHHHLH HHHHHHUHUHHUHHHHHHHHHHUHHHHHHHHHHHUHUHHHHHHHHH HHHHHHHHHL HHHHHHHHHLH HHHUHHHHHUHHUHHHHHHUHUHHHUHHHHHUHHHUHHHHHHL HHHHHHHHHHHHUUHUHHHHHHHHHHHHHHHHHH HHHHHHHHHUHUHHHHUHHHHHHHHHUHHUHHHHHL e HHHHHHHUHUHUHHHHHHHUHHHHHUHHHHHUHHHHHHUHHUHHHH HHHHUHLHHHHUHHHHUHHUHHHHHHHH TD3010JE_v3 00D 53 TP600 TP650 e UL 0 000000000000 00 00000 TaKaRa PCR Thermal Cycler Dice TP600 Gradient TP650 Standard 0000 0 00 0 nuc HH 0 000 HHHRH 000 moog HHHH 000 C 0 HHHH 000 910000 0 HH Hsec 0 DDmininsec 0 gt 00000000 HHHHHHHHHHH HHHHLHH HHHHHH OOOO 60000 000 00000000000 108 000 OOO L HHHHHHH 00000000000 0 00 00000 iuo 0000 0 0 D 00007 1 IILI GI 000 bue O OD mue 000 OD 0 0 0000 00 0000 00 0 0 0 00 D U U 085 0000 HHHHHHHHHL D UD 0 000000 0 00001 aue OOO DDD GI bud 0000000000000 00000 0000000 DU 00000 0 MUU QAI 000000000 HHHHHHHHLHHI AC 000 0 000 4 DUUVA CR202 IHH _ 000025 000090 rm o 0 CAN CSA 22 2 No 10101 1 92 No 1010 1B 97 UL Std No 61010A 1
56. HHHHHHHHHHL nnnn 0000000000 Installation Precaution HHHHHUHHHHHL Pr cautions d installation 00000 eris HHHHHHHHHHL daba oo HHHHHHHHH 00 Ne Pas Ouvrir en cours de LL ET ET ED D HHHUUUHUU RR TD3010JE_v3 00D HHHHHHHHH 0000000 000000 ora 00 0660 00000 0 0 00 il illi Copyright TAKARA BIO INC Printed in J 0 000 0000 00 0 00 0 00 00 0 00 0 000 0 520 2193 OU 41 i HHHHHHHHHHU 000 Tel 077 543 7231 TD3010JE_v3 00D Precautions tor Using TaKaRa Scientific Instruments Users are recommended to carefully read the content of this manual before operating the TaKaRa PCR Thermal Cycler Dice system e After familiarizing themselves with the information in this manual users are recommended to store it in secure and convenient location from which it is always available for reference Introduction All Takara scientific instruments are sold strictly for use in research applications and should not be used by those who are not experts in this field Due to ongoing improvements product specifications and the external appearance are subject to change without prior notification Warranty and Responsibilities Refer to the Warranty card included with this
57. HHHHHHHHHL CSA RoHS CSA S ED E eI RO Canada Standard Association UL UL Under Laboratories CE EU EB BENE Communaute Europe enne RoHS EUQQ 000 UDduuuututuluttl Hestriction of the use of the certain Hazardous Substances in electrical and electronic equipment TD3010 E v3 00D TP600 TP650 iUil lllull nib Lu TU AL TAA Y 1r FID D OO DL L AAA OOOO TMI OOOO OT TTA EE SH TAN 00000000000 OND Morton 00 1000 iu 000000000 0000000010000 000 000000000000000000 eut HHHHUHHHHHHHHHUUUHUHHHHHUHHHHHL 0000000000000 110HTHHHHHHHHHHHHH 910000000 0 000 000 0000 00000 0 0900910 00000000000 e miu HHHHHHHHL eu HUHUHHHHHHUHHHHHHHHHHLH HHHHHHHHL UUUMUU TD3010JE_v3 00D TP600 TP650 HHHHHHHHHHHHHH 000000000000000000000000000000 000 eu 000000 oNIOI FOFFITI 00000 000 0 000 910100000 0000000000000 a EEUU GE E A Y YY AN n XX AA 45 3 V Y YA M AP AAA 44 AAA NN AX AN AA AAAS YY A Y AN
58. HHHHHHHHHUHUHHHHHHHUHHHHUHHHHL 000 0 0 00 00006 HHHHHUHHHHHHHHUHHHHHHHHHHHUHHHHHHHLL HOU 159 30 CQOU U WOO 2910 89 HHHHHHHHLH HHHHHHHHUHHUHHUHHUHHHHHUHUHHUHUHHHL HHHHHHHHHHHUHHHHHUHHHHHUHHHHL HHHHHHHH OUHHHHHHHHHHHHHHHHHH 10000 HHHHHHHHL HHHHHUHHHUHHHHHHHHHHHHHHHUHL HHHHHHHHHHHHHHHLHHHHL Lll onda da 000000000000 00 0000000 000001011010 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHL HHUHHHHHHHHHHL TP600 TP650 000 000 00 00000 9000000000000 0000 0000 000000 000 2 HHHHHHHHHHHHHHHHHHHHHH 0 0 0000 00000 000 00000 0 00000000000 00 0090 0 00000000 000000000000000000 0000000000000 HHHHHHHHUHHHHHHUHHHUHUHHUHUHHHHHHUL 00000000000 0000000000 00 0000 0 0 0 0 00 0000000 000000 Select User Name O 0 0 lt gt aKaRazt O O I 0 0000060 HHHHHHHHHHHHHHHHUHHHUHHHHHHHHL e HHHHHHHUHHHH et 1 000000000000000000000000000000000000 000 00 0000 L 2 000000000000000000000000000000000 3 0000000 000000000 00000 000 00000060 4 00000000000 00000 00000000000000000 5 0000000000000 000 00000000000000 TD3010 JE_v3 00D TP600 TP650 000 JUUUUUUUUL HHHHUHUHHUHHHHHHHLH D OU 0000 000 m 10000 HHHHHHHHHH
59. HHHLH Long LINK D DO OL UL B DTE LINK Short LINK 0000000000 LINKE LOO QUO UU OOo T HIHHHHHHHHHUHHHHHUHHHHHHHHUHHHHHHHHHHHHL 90000 0 000019000 00000000000 0000000000 HHHHHHUHHHHHHHHHUHHHHUHHHHHHHHHHHHHHHHHHH UUUHHHUL HHHHHHHHHHHI 010000000 0000000000000000 LINK LINK LINK LINK LINK LINK Long LINK Long Short LINK Short LINK Short LINK LINK LINK TD3010JE_v3 00D 34 TP600 TP650 LITLEELELELT ELTE I ET 1 000000000000000000000000000 00 0 000 0000000000000 RUNI UU E CLE D O O 0 0 0000 060 0000 Select User Name lt TaKaRa gt MENU Sabro OK New MENU JOK Enter TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx RUN User Common 05 05 05 12 00 00 25 0 C 24 HHHHHHHHHHHHHHH PLANT TEST PLANT TEST HOUUU 0 SEIL EE 0 0 0 00 0000 PLANT TEST PLANT TEST 000 0 BEPEANT TEST TN 0 OOKO Enter 3 000000000 00 0 Enter Select Reaction Moda 0 Mode E Container i 2 Thick Volume 50 ul 5 150 HI Link 1 1 Yes 2 No 4 000000000000000 000000 LnkHHHHLHHHHHHHHHHHHHHHH MENU Enter TD3010JE v3 00D 35 TP600 TP650 5 000 0 Start EnterHHHHHH 00 OPLANT TEST CL 0000 UD PLANT 000 UO 0 0 DPEANT TESTSEILEI OOO UO OPLANI TESTAEILEHI 00
60. IO INC SETA3 4 1 OTSU SHIGA 520 2193 JAPAN Tel 81 77 543 7247 Fax 81 77 543 9254 http Awww takara bio com Technical Inquiries Contact htto Awww takara bio co jp english contact inquiry_form htm TD3010JE v3 00D TP600 TP650 HOU DU TD3010JE v3 00D TaKaRa 0000 000 L UU UU OOO 00 D 000 L 000 0 HHHHHHHLH HUU U 000 DU 000 C 00 00 0 0 0 0 0 DO O O O 00 LI 00000 00 0 0 0 TI DU 0000 00 B 0 000 0 0 0 0 0 000 0000 00 0 Ungu 000000 0000000 00000 000 00000 00000 0000000 0000 000 000000000 HUU 00000 0000000000 0000 UU 000000000000000 OOOO 0 0 0000 rtl UU HHHHHHHHUHHHHL 1000 2000 3000 E EHE 40 0 LI 6001 Ji 101 MOOG 16101 17100 23 280 UL 4000 380 00 390 0 0 41000 470 00 500 gt 40 D E 560 O L 580 I LI TP600 TP650 UU TaKaRa PCR Thermal Cycler Dice HDD TPeo GRADIENT 000000000 00 0 TP650 STANDARD 0000 TaKaRa PCR Thermal Cycler 10 0000000 00000000000 00000000000 000 0000 1 0000000000 mTP HII 00000 0 OOOUU UO000 Hr 00 0 0 000 00 0 000 00 000 0 0 000 0 IIHHHHHHHHHH 260
61. O 000 0000 0000 O 000 0 000 0 D Enter 00 ExHHH 000 00000 000 O IIHHHHHHHHHHHHHHHHHHU Tire 00 008 00 00000 0100000000000 Enter TD3010JE_v3 00D TP600 TP650 HHHHHHHHHHHHHHHIHHUHUHHHHHHHHHHHHHHHHHL HHHHHHHHH HHHHHHHHHHHHHOHHHHHHHHHHHHHHH HHHHHIHHHHHH Dp pn b pag HB OL DID EE BELEB E BEU E UE C E OWO 0000 0 O OO 000 00 0 DU UU 00 D HE 0 0 0 DD 000 000 DUENTER TE O 00 HLHHHHUHHHHHHHHHH HHHHHHL O 000 000 00 0 0000 000 00 0 0 0 0 0 DUENTER TE O 00 1Temp 3Temp 25cyc 2 Te MENU 1 Time 10 00 Ex 0 00 0 00 O INOTEH 0000000000000 cE 0000000 0000000 Pausen OOOO 000 00 00000 0 0 Pause Ege a NOTE 0 00 0 0 0 0 00 00 0 00 0 000 00 000 0 0 0 00 00000 NOTE 0000 00 0 0 0 000 00 0 00 00 00 0 0 0 0 0 0000 00000 0 000 00 000 0000 00 L Save Delete Gradient 0 000000 00000 Temp Ex 0 000 05 7 05 00 U 0000 00000 Time Exp 000 OSave Cancel D 0 00000 TP650 TP600 HEN TD3010JE v3 00D 20 TP600 TP650 0000000000 UP650HHHHHHHHIHHH 000000 0000 0 0 00000 000000000 Grad Enter i1Tempi3Temp 25 2 Te MENU 1 HHHHHUHHHHHHHHHHHHH Gradient 02 46 03 47
62. Setting in Tool Menu and check LCD sleep setting Go to rear of display and adjust Display Brightness Knob Remove the foreign object or matter Confirm the nature of the error and request system servicing as required TP600 TP650 Operation Manual Power failure Operation will be resumed from the stopped state and a program will be ended When a heated lid becomes by power failure below in a predetermined temperature resume operation after warming a heated lid to an appropriate temperature When you operate in your absence at night and an experiment is completed Please check the following method whether abnormalities have occurred 1 Operation End Screen Display Finished ABC Test User lt Common gt Started at 09 47 12 05 01 20 Finished at 09 54 32 05 01 20 Length of Run Over 0 0 Result Power failed Power Failed It is shown during operation that there was a power failure At the time of a normal end It is stopped when Normal and operation are stopped on the way 2 History Display MENU Tools gt History See page of 96 Three events of total six events User lt Common gt Program ABC test Mode 1 Thin 50 ul Started at 09 47 12 05 01 20 Power Failed 09 50 34 05 01 20 Recovered at 09 50 37 05 01 20 The first time of restart The first time of stop Last events of total six events Power Failed _10 25 10 05 01 20 The second time of power Recovered at
63. UUUUUU 00 000 00 000000000000000000000000000000000000000000 UUUUUUUUUUUUUUUUDUUUUU This Warranty covers repairs that shall be made at no cost to the customer for the period listed above For information on the cost of repairs after the Warranty period please to consult with a Takara Bio Inc branch or local sales agent If there is no the TaKaRa s seal in this warranty card this warranty is invalid HHHHHHHHHH UU Sales Agent TAKARA BIO INC 0 000 0006 SS Seta 3 4 1 Otsu Shiga 520 2193 Japan K k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k k Kk k k k Kk k K k KK 09 99 09 99 0 9 99 0 000000 http wwtakare 103 8232 HHHHHHHHH 200 15 10 TEL 02 3271 8553 FAX 03 3271 7282 0 520 2193 00000 00300 41 TEL 077 543 7297 FAX 077 543 7293 00000 0 525 0055 000000000 2257 TEL 077 567 9261 FAX 077 565 6995 0 520 2193 31041 TEL 077 543 7231 FAX 077 543 9254 TAKARA BIO INC SETA3 4 1 OTSU SHIGA 520 2193 URL http wwwtakara bio com 2009 01 3 gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt
64. a setting It becomes Gradient temperature setting screen 1Temp3Temp 25cyc 2Te MENU 4 Save Insert Delete Grad U Cancel It becomes Gradient temperature setting screen Gradient Setting 02 46 0 03 47 0 04 49 0 05 51 4 06 53 8 07 56 2 08 58 6 09 61 0 10 63 0 11 64 0 65 0 the low temperature setting at position 01 The left end of a block and the high temperature setting at position 12 The right end of a block Temperature setting range 40 0 to 75 0 C Temperature difference 6 0 to 20 0 C Note Exceeding the temperature settings or the temperature setting range may destabilize controlled temperature The temperature values for 02 to 11 are calculated values Temperatures cannot be automatically incremented decremented for each step Go to pop up menu select OK and press ENTER When displaying the temperature gradient the left temperature is shown on the Temp line and the right temperature beginning with G on the Temp Ex line Go to pop up menu select Save and press ENTER TD3010JE_v3 00D 18 TP600 TP650 Operation Manual Save Time 10 00 0 30 0 30 0 30 5 0 00 3 Save the newly created program Entering program name Save Program as SaveAs Program Cancel User Common Go to pop up menu select SaveAs and press Notel User created programs maximum memory capacity
65. aining 0 02 W CNE Start Cancel Results Displayed Result of Rate Test MENU Heat 3 1 C s Cool 2 1 C s Over 0 1 C UnderO 4 C Started at 15 47 21 03 01 15 Finised at 15 50 52 03 01 15 Length of Run 00 03 31 Resul t Nor mal Reference Values Max Heating Rate gt 3 0 C second Max cooling rate gt 2 0 C second 2 Cycle Test Program execute screen Program details 94 C for 30 seconds 55 C for 30 seconds and 72 C for 30 seconds Remaining 0 02 Start Results display Result of Cycle Test MENU Heat 3 0 C s Cool 2 1 C s Over 0 2 C 4 Started at 15 39 09 03 01 15 Fini sed at 15 42 39 03 01 15 Length of Run 00 03 30 Resul t Nor mal TD3010JE_v3 00D 102 TP600 TP650 Operation Manual Reference Value overshoot lt 0 5 C undershoot lt 0 5 C cycle time lt 4minutes 3 Up Down Test program executes cream Program Details 98 C for 1 minute and 4 C for 1 minute Remaining 0 02 Start Cancel Results displayed Result of Up Down Test MENU Heat 3 2 5 Cool 1 4 0 2 UnderO 3 C Started at 15 59 23 03 01 15 Finised at 16 05 49 03 01 15 Length of Run 00 06 26 Resul t Nor mal Reference Value cycle finish time 10 min 4 Gradient program test model TP600 only Program Details 94 C for 30 seconds G50 G60 for 5 min and 72 C for 1 minu
66. ame Select OK on the pop up menu and press ENTER 3 Enter password when the password entry screen appears User Jiro Cancel tk Password length to 9 characters that appear as when entered Use the CE key to clear password If the password is mistyped use the CE key to clear and re enter password 4 Select OK on the pop up menu press ENTER and return to the top screen TD3010JE_v3 00 86 TP600 TP650 Operation Manual 2 Registering New User 1 Goto main system screen pop up menu select User and press ENTER TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx RUN New User Common Edit Tools User 05 04 20 12 00 00 25 0 2 Select New on the pop up menu and press ENTER Select User Name MENU lt Common gt lt TaKaRa gt New 3 Enter the new user name go to pop up menu select Save As and press ENTER save MENU MENU User Jiro 4 Enter the password if necessary User Jiro Cancel Password aww Enter password Password characters and length numbers 0 to 9 1 to 6 digits This displays the entered numbers on the screen lf the numbers are mistyped use the CE key to clear and re enter password To avoid registering a password simply leave the field blank select OK from the pop up menu and press ENTER TD3010JE_v3 00 87 TP600 TP650 Operation Manual 3 Changing Existing Username 1 Goto pop up menu select User and
67. anged main system screen is displayed TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx RUN New Tools User 05 01 01 12 00 00 25 0 lt NOTE gt Username Edit Functions Up arrow key insert space Down arrow key backspace Left arrow key cursor left Right arrow key cursor right Character input keys Okey amp 0 1 key Space 1 2 A B C a b c 2 D E F d e f 3 4 GH l g h 1 4 5 J K L j k 1 5 6 key 6 7 key PQ R S p g r s 7 8 key TU V t u v 8 9 W X Y Z w x y Z 9 Enter only numbers for number fields TD3010JE_v3 00 89 TP600 TP650 Operation Manual lt NOTE gt Pre registered user names Common and TaKaRa may not be changed The following protocols are registered to the lt gt folder lt 1 lt 5Kbp lt 10Kbp gt 10Kbp Mutagenesis siRNA double Differential DD and BcaBEST RNA Preset Protocols These consist of programs preset protocols programmed for use according to the length of the target lt 1 upto 1 Kbp 94 C for 30 s 55 C for 30 s 72 C for 1min at 30 cycles 4 lt 5 up to 5 Kbp 94 C for 30 s 55 C for 30 s 72 C for 5min at 30 cycles 4 C oo lt 10 up to 10 Kbp 98 C for 10 s and 68 C for 10 min at 30 cycles 4 gt 10 more than 10 Kbp 98 C
68. ays the main system screen described in step 3 Users logged on as TaKaRa are not able to perform program save operations change username There are eight standard protocols registered for the TaKaRa user name Their program names lt 1Kbp lt 5Kbp lt 10Kbp and gt 10Kbp Mutagenesis sIRNA double Differential DD BcaBEST RNA See Chapter 4 Note Preset Protocols on page 90 3 Starting a System Operation Program If a system operation program does not yet exist begin by going to section 5 Creating New Programs or 6 Editing and Saving Existing Programs With the main screen displayed press the MENU key select Run from the pop up menu and press ENTER TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User Common 05 02 14 12 00 00 25 0 C This displays the names of programs registered to the user chosen from the Select user screen Use the arrow keys to select the desired program press the MENU key then go to pop up menu select Start and press ENTER P N St d rogram ame ore MENU RT PCR 05 04 01 Start Cycle seq 05 04 02 D TaKaRa Taq 05 04 02 Touch Down PCR 05 04 02 Notel When the pop up menu is active the arrow key does not function Press key to disappear the pop up menu and select program by Using arrow key Note Other functions in pop up menu 1 Checking Program Information To confirm the program informa
69. cket Fault in internal system circuitry Note that power switch includes an over current breaker Operating environment temperature may be unsuitable Electronic cooling element may be damaged Operating environment temperature may be unsuitable Electronic cooling element may be damaged Faulty temperature sensor This is due to running the test before lid heating time is up Therefore the time required to complete the test exceeded the allowable time Beeper setting may currently be Faulty keys or keypad wiring LCD sleep setting may be set to 30 min Faulty backlight Faulty LCD Backlight brightness not adjusted properly Foreign matter between lid heater and sample block Faulty lid lock mechanism Refer to list of error codes 108 Check power cable connections on system and power outlet sides Check installation facility power supply Immediately disconnect the power cable and have the system serviced Operate the system ina temperature environment of 2 to 23 C and run self diagnosis tesis If outside rated performance have the system serviced Operate the system ina temperature environment of 2 to 23 C and run self diagnosis tesis If outside rated performance have the system serviced Allow system to warm up for at least 10 minutes then rerun the test Go to Default Setting in Tool Menu and check the key entry sound setting Go to Default
70. d carrying the system be sure to place one hand on either side of the system and lift and carry in a balanced way 4 Make sure that the new location conforms to the installation requirements TD3010JE_V3 00 62 TP600 TP650 Operation Manual described in section 1 Installation Conditions in Chapter 2 5 Follow the installation procedures outlined in section 2 Installation in Chapter 2 e Moving to a New Site When moving the system to a new site clean it carefully and repack it in the packaging in which it was delivered Note that damage sustained by the system during transport due to improper packaging will not be covered by Warranty even if the Warranty period is still valid Follow the instructions provided in this chapter when reinstalling the system at the new location 4 Maintenance Be careful especially of the following point before beginning maintenance check 1 Especially the maintenance work without directions of operation should surely turn off a power supply switch for safety 2 Warn to spill neither water nor a reagent to mechanism parts and electric parts Moreover please place neither detergent nor medicine on equipment 3 Please do not touch with a hand other than the appointed place e Routine maintenance work 1 Please clean a main part periodically and suitably 2 Wipe all outside surfaces of the equipment with a diluted neutral detergent solution 3 When a liquid is spilt pleas
71. e Reaction Mix 10x One Step RNA PCR Buffer 5 Ll 25mM MgCle 10 H dNTP Mixture 10 mM each 5 H RNase inhibitor 40 U ul 1 H J AMV XL 5 U ul or DEPC treated water 1 Lu AMV Optimized U ul 1 Control F 1primer 1 20 uM 1ull Control R 1primer 2 20 uM 1ull Positive Control RNA 1 Lu RNase Free H2O 24 ul Total volume 50 H Conditions M 1 2 M 2 Result 50 15 min RT reaction 94 C 2min jnactivation of RTase 949C 30 sec 30 cycles 60 C 30 sec PCR 72 1 5 min Lane Sample 1 RTase 2 M 100 bp DNA Ladder Dice Lane E6 Dice SP Dice Lane 7 TP600 TP400 After the reaction was completed a portion of reaction was applied for agarose gel electrophoresis In both cases using TaKaRa PCR Thermal Cycler Dice Cat TP600 and TaKaRa PCR Thermal Cycler SP control reaction product 462 bp was detected only under the existance of RTase TD3010JE_v3 00D 115 TP600 TP650 Operation Manual Application 3 Comparison of 8 kbp A DNA Region Amplification for TaKaRa PCR Thermal Cycler Dice vs SP e Evaluation target Compare differences in 8 kbp DNA region amplification Dice TP600 SP TP400 between TaKaRa PCR Thermal Cycler Dice TP600 and TaKaRa PCR Thermal Cycler SP Row B Reaction tube TaKaRa Micro PCR Tube Cat 9047 e Reaction mode 2 Selected at PCR Thermal Cycler Row c
72. e wipe off a sample block circumference part as early as possible 4 Since dust is easy to collect please clean a sample block frequently If dust and garbage adhere to a sample block a temperature control performance will fall and temperature homogeneity will also become bad simultaneously 5 Please check it that the air hole of the back of equipment the left and right hand side is not closed Please wipe off when dust has adhered Neither strong alkali high concentration alcohol nor use of the C AUTION organic solvent can be performed It becomes the cause of breakage such as discoloration deterioration and a crack TD3010JE_V3 00 63 TP600 TP650 Operation Manual Chapter 3 Names of Parts and Their Functions Front View 1 Lid Handle For sliding use with lid open 2 Lid 3 Sample Block 4 Displa 5 Key Pad 6 Control Panel 7 Air Vents Front 9 Power Switch 10 Air Vents 8 Control Panel Release Lever ON H OFF O Left and Right Sides Pressing upward releases lock General Description F 1 Lid Handle Be sure to perform sliding operations with the lid open lt Caution gt Do not lift the unit using the lid handle 2 Lid The lid is designed to protect against evaporation resulting from heating of the reaction tube and reaction plate lt Caution gt Avoid inserting fingers as the interior of the unit reaches very high temperatur
73. echnical support line CAUTION Do notsit on the system Do not disassemble the system TD3010JE_v3 00D LL Warning Safety Labels Applied to System Case Users are advised to avoid accidents by carefully reading the warnings and cautions contained on warning stickers at key locations on the interior and exterior of the system Warning Type Location of Danger Type d Avertissement Localisation du Danger Possible Danger Disassembly Prohibited System Interior Demontage Interdit Int rieur de Appareil Electric shocks and fires Warning High Voltage Power cable Attention Haute tension Cordon d alimentation Electric shocks Grounding Mandatory Power cable Mise la Terre Obligatoire Cordon d alimentation Electrical shocks and fires High Temperatures Heat cover amp Heat block Haute Temp rature Couvercle Chauffant Installation Precaution Air vents Pr cautions d installation Prises d air Burns and fires Do not Open When in Operation System cover Ne Pas Ouvrir en cours de fonctionnement Couvercle Personal injury TD3010JE v3 00D Warning Label Disassembly Prohibited D montage Interdit Warning High Voltage Attention Haute tension AREE Grounding Mandatory Mise la Terre Obligatoire High Temperatures Haute Temp rature Installation Precaution Precautions d Installation Do not Open When in Operation Pas Ou
74. ecified interval after 1 30 or 2 00 minutes seconds The LCD is designed to wake up by pressing MENU Will not wake up with other key operation Buzzer Key Pad pressing key toggles options 1 beep on 2 beep off Buzzer Finished selects between options 1 beep on 2 beep off Beep count 3 at program pause 6 at program finish 3 PC Communications Function Program Send and Receive This function is used to save program data to a computer or send it from a computer to other devices It is also used to perform system backup and transfer operations 3 1 PC Communication Settings Windows 2000 and XP Version 1 From Start Menu Go to Programs Accessories Communications and open HyperTerminal If not included in the Start menu open HyperTerminal by going to the following directory C Program Files Windows NT hypertrm exe 2 HyperTerminal Screen New Connection Name Example TaKaRa Connect using COM1 COM Properties gt Port Settings Bits per second 9600 Data bits 8 Parity Stop bit Flow control Xon Xoff ASCII Setup go to File Properties and select the Settings tab Click ASCII Setup button and enter the following settings Line delay 2 milliseconds Character delay 0 milli seconds Uncheck all other options TD3010JE_v3 00D 98 TP600 TP650 Operation Manual When Receiving go to Transfer and select Capture text then specify file name and click the Start button When sending go to Transfer sel
75. ect Send Text File and select the send file name Important Notes gt The RS232C cable with 9 pin D sub connector required for this feature is not included as a standard accessory 3 2 Transferring a Program to Computer Dice 1 Choose PC Communication go to pop up menu select OK and press ENTER Select Tools MENU History OK Setting SETS PC Communication Cancel Diagnosis Heserved Delete User Data 2 Choose Send a program to PC go to pop up menu select OK and press ENTER PC Communication MENU OK Send a Program to pc Receive a Program from pc 3 This displays the program list Choose program to send go to pop up menu select OK and press ENTER Program Name Stored MENU MENU Cycle Seg 03 04 01 TaKaRa Taq 05 04 01 Touch Down PCR 03 04 01 4 On the PC use HyperTerminal In the HyperTerminal screen to go to Transfer and select Capture then save the file by clicking the Start button For a description of the PC settings refer to PC Communications Settings 5 Transfer the desired program MENU Send a program to pc Program Name lt TaKaRa Taq Finished Go to pop up menu select Start and press ENTER The Finished message appears when transfer is complete Select Cancel from the pop up menu then press ENTER to be returned to the main TD3010JE_v3 00D 99 TP600 TP650 Operation Manual menu screen 3 3 Transferring Programs From a Computer
76. ely turn off power to the system and all connected devices and disconnect the power supply cables Avoid blocking the paths to any air vents included on the product Blocked air vents can lead to internal overheating that may result in physical burns or fire Avoid installing the system in locations subject to dust or strong CAUTION vibrations Avoid installing in locations subject to direct sunlight and wide temperature fluctuations as this may result in internal overheating and may cause physical burns or fire Do not place objects on top of the system Do not install the system in locations other than research institutions or related facilities Electrical Precautions Observe the following precautions when using electrically powered scientific instruments in order to prevent electric shocks and fires Avoid operating system with power sources that do not conform to WARNING system power supply specifications Avoid sharply twisting altering marking or otherwise damaging the power supply cable Avoid applying stress to heating or placing objects on the power cable Always ground the system to prevent electric shocks due to leakage Current TD3010JE_v3 00D Be sure to compare system power supply requirements with the rated power capacity of the power source to ensure system current requirements do not exceed power socket capacity Confirm that the total power requirements of all connected e
77. em standby temperature display screen color and highlighting system sleep time and sound settings 2 1 Time and Date Settings 1 Choose Setting then go to pop up menu select OK and press ENTER 2 Choose Date amp Time then go to pop up menu select OK and press ENTER Date amp Time i Default Setting 3 Enter display format and date time settings then go to pop up menu select OK and press ENTER IFLA Y MD 2 M D Y 3 D M Y 03 01 15 14 15 00 TD3010JE_v3 00D 97 TP600 TP650 Operation Manual For example the time 14 15 00 and date January 15 2003 are entered as follows Date Style 030115 Time 141500 Then go to pop up menu select OK and press Enter key 2 2 Default Settings 1 Choose Setting then go to pop up menu select OK and press ENTER 2 Choose Default Setting then go to pop up menu select OK and press ENTER Default Setting Stand by Temperature LCD Back Color 1 LCD Sleep 1 T Buzzer Key Pad 1 1 Buzzer Fini shed 1 1 Enter the desired settings go to pop up menu select OK and press ENTER Stand by Temperature block temperature setting during standby mode default is 25 C LCD Back Color screen background settings 1 blue 2 white character color complements background color LCD Sleep enables system sleep mode shuts off display backlight when there has been no input while program is idle for a sp
78. en the system is paused Error messages are displayed on the main system screen when errors are detected during standby mode 1 Events and Error Code Table 0 Sma Indicates program start ime 1 Finished at indicates program finish ime Stopped at indicates time program stopped by user 3 Paused at Indicates time of system pause Resumed at Indicates time system operation resumed 5 Power failed at indicates time of system power failure 6 Recovered at indicates time of recovery from system power failure e Coorme indicates abnormal cooling rate Overshoot Indicates overshooting Note that only one error is displayed at a time even if several occur simultaneously TD3010JE v3 00D 107 TP600 TP650 Operation Manual 2 Troubleshooting ___ Symptom Cause System fails to operate and display is off even when power is applied Power switch stays off even when turned on Heating and cooling rates are abnormally low Cycle time is too long The See operation manual message 1 displayed as the self diagnosis test result The normal key entry sound is absent The display goes off Display is too dark too light Lid will not close Error number appears TD3010JE_v3 00D Power is not coming to the system Power supply cable is not properly plugged into the socket Power is not coming to the so
79. eration Manual TaKaRa PCR Thermal Cycler Dice TaKaRa SP __ gt o IAl 1 1 TD3010JE_v3 00D 118 TP600 TP650 Operation Manual Appendix 3 Please copy and use this Servicing Request and Safety Check Attention Distributor of Takara Bio Inc Product Name TaKaRa PCR Thermal Cycler Dice Model TP600 TP650 Serial No Date of request Requested by Name Contacts Telephone Email Company Institute name Address Details of request Current status Customers are requested to provide the following information on the type of the function and any safety considerations Safety check covers all personnel involved in using the system as well as safety of the location in which the system was operated If contaminated the customer is requested to decontaminate the system prior to servicing IPathogenicity Onone possibly possibly Radioactive substances not used usedl nuclidel I 2 System contamination status Lino contamination decontaminated possibly contaminated This is to confirm that the system is free of contamination O This is to confirm that there is risk of contamination and that specific safety measures and decontamination procedures were implemented Date year month day Company Institution Witness by signaturel AN warnine This Safety Confirmation Form
80. erature diagonal line section of graph flashes Stabilized temperature horizontal line flashes Temperature indication HOT is flashing when over 50 C Time remaining for current step counts down Definition of o continuous operation 99 99 Notel The Lid is warming up xx x C message appears if it has not reached 108 C over temperature The System starts as soon as the lid is warm 1 Pausing When System Operation Go to pop up menu select Pause and press ENTER 1 25 Remai ni ng 1 22 MENU To resume system operation select resume from the pop up menu and press ENTERI 1 25 Remai ni ng 1 22 oe TD3010JE_v3 00D 72 TP600 TP650 Operation Manual 2 Viewing Program Information During Operation While program 1 running go to pop up menu select Info and press Program Informatlon User Common Program TaKaRa_Taq Mode 1 Thin 50 Ll Run Started at 09 47 12 05 02 20 Run will end at 09 54 32 05 02 20 To close Info screen go to pop up menu select OK and press ENTER Terminating or Aborting Operation Press key ll This displays the following confirmation screen C onf i r mat ion Are you sure to stop cancel Temperature 54 6 C 00 Go to the pop up menu select OK and press ENTER This displays the following termination screen Finished TaKaRa Taq User Common Started at 14 14 23 03 01 15 Finished
81. es up to 110 3 Sample Block Section of the unit in which sample tubes and plates are placed lt Caution gt Do not touch the block as it reaches temperatures up to 110 lt 4 Display Displays current status of the various system features and functions TD3010JE v3 00D 64 TP600 TP650 Operation Manual 5 Key Pad Used to enter the various programs 6 Control Panel Enables adjustment of various angles gradients 7 Air Vents Front Openings used to cool the interior of the unit 8 Control Panel Release Lever Used for changing the angle of the control panel Pressing upward releases lock 9 Power Switch Power switch is equipped with circuit breaker 10 Air Vents RandL Openings used to cool the interior of the unit lt Caution gt Do not block any of the air vents Rear View 13 Brightness Adjust Knob 11 Power Cable connector A A YA VIN A Y MAAAAAAA AA 4 A A ry X XXX AA Y Ww XX OO A A Q X 0 A 0 OO AAA OO NA NA UM A A M 12 Air Vent rear T A A Y A 14 Communications A A A Y A 4 X m Port Connector General Description nP sM AF 11 Power Cable Connector Socket for power cable lt Caution gt Make sure to use the powe
82. extension setting can be canceled by pushing key or entering 0 The Pause setting can be canceled by pushing key or going to MENU and selecting Pause again Note Decreases every five seconds but It doesn t become less than one second Notel Pop up menu content varies according to cursor position The pop up displays Save Insert and Delete when the cursor is positioned at Temp or Time Save Slope and Cancel are displayed when positioned at lempE Save Pause and Cancel when positioned at TimeEx TP650 TP600 Save Grad Cancel Cancel 3 Slope Function Settings With the cursor placed on TempEx go to MENU select Slope and press ENTER This displays the following Slope screen wewu Slope Setting Range TD3010JE_v3 00D 77 TP600 TP650 Operation Manual Temperature 30 0 to 99 9 C Time 1 30 1 minute 30 seconds to 99 59 99 minutes 59 seconds INote The Minimum Time Setting for Slope is 1 min and 30 s Any setting shorter than the minimum setting will automatically be adjusted to this value Note Slope setting is canceled by moving the cursor to the designated position and pressing the key or going to MENU selecting Slope and pressing again These steps reset the current slope setting to its default value 0 0 4 Gradient Function Settings only for TP600 With the cursor positioned on Temp or Time go to MENU select Grad and press ENTER to select
83. he former program remain T INote The message of Change Program Name appears when SaveAs is selected without changing the name and is done and the program name change is demanded Please input a new name Note Programs cannot be saved to the TaKaRa username using the Save or SaveAs functions Select User from the pop up menu and save the program under a different username INote User created programs maximum memory capacity for programs 6 size is 200 Program name character Maximum 16 Maximum number of registered users 22 excluding pre registered Common and lt gt usernames TD3010JE v3 00D 85 TP600 TP650 Operation Manual 7 User Selection and Registration Options include 1 Select user name User 2 Register new user New and 3 Change user name Editor 1 Select user name 1 Goto main system screen pop up menu select User and press ENTER TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx RUN New User Common Edit Tools User 05 04 20 12 00 00 25 0 2 This displays the Select Username screen Select User Name lt Common gt lt TaKaRa gt Sabro Cancel lt NOTE gt This screen appears at system startup when the power is turned on The defaulted usernames Common and lt TaKaRa gt are pre registered Maximum number of user names 22 Maximum user name length 10 characters Use the arrow keys to select the desired usern
84. icates the time that the first program commenced and Finished at the time the last program was completed TD3010JE v3 00 95 TP600 TP650 Operation Manual Chapter 5 Diagnosis Functions and Other Tools The following functions are provided in the Tools menu 1 History records history of activity 2 Settings enters time and date and system default settings 3 PC communications sends and receives programs 4 Diagnosis runs operation testing programs 5 Delete user data deletes saved usernames and programs gt NS gt lt gt Selecting Tool Menu Go to main system screen pop up menu select Tools and press ENTER TaKaRa Thermal Cycler Dice TP600 Ver X XX User Common Tools 03 01 15 12 57 31 Choose desired item from the displayed Select Tools menu select OK from the pop up menu and press ENTER Select Tools History Setting PC Communi cation s Diagnosis Reserved Delete User Data lt NOTE gt The descriptions of the various functions provided below assume that they have already been selected from the Select Tools menu by selecting OK in pop up menu and pressing ENTER 1 History Function History of Events The History function records a log of activity for the most recently executed program Executing a new program updates the history log by overwriting previous data Select History choose OK from the pop up menu and press ENTER User
85. imer 2 20 u M 0 5 gll A 0 5 ng u 1 Ll Sterilized water 38 75 H Total volume 50 H PCR Conditions 2 step 98 C 10sec 30 cycles 52to72eC 5 min y Gradient temperature of 20 C Gradient 3 step 98 C 10 sec 45 to 65 C 30sec Gradient temperature 20 C 72 C 5 min 30 cycles TD3010JE_v3 00D 113 TP600 TP650 Operation Manual Results Results for 2 step PCR 2 step PCR annealing extension temperature M 52 C gradient 72 M A Hind Ill marker Higher annealing extension temperatures result in reduced low molecular nonspecific amplification and increased amplification of the target amplicon Results for 3 step PCR 3 step PCR annealing extension temperature 45 paden 65 C M A a w a o w o i a w a M A Hind Ill marker Higher annealing extension temperatures give reduced low molecular nonspecific amplification and increased amplification of the target amplicon TD3010JE_v3 00D 114 TP600 TP650 Operation Manual Application 2 One Step RT PCR The performance of TaKaRa PCR Thermal Cycler Dice Cat TP600 was evaluated through One Step RNA PCR by using TaKaRa One Step RNA PCR Kit Cat RRO24A in comparison with TaKaRa PCR Thermal Cycler SP RI PCR was performed in two conditions In the presence of AMV XL in the reaction mixture Without AMV XL in the reaction mixtur
86. ks can be inserted or deleted by going to the start position Example placing cursor on 1 of 1 Temp block and selecting Insert Delete from the pop up menu Save Insert Segments can be inserted deleted by placing cursor anywhere except the pattern start position Example placing cursor on 55 0 0 30 or 72 0 1 00 for 3 Temp 25 on the screen below and selecting Insert Delete from the pop up menu TD3010JE_v3 00D 80 TP600 TP650 Operation Manual MENU 2 1Temp3Temp 25 yc MENU 1 Run x Save 94 0 94 72 00 72 Cancer 10 00 0 5 07 Switch Pattern Temp count can be modified by inputting a new value 1 to 9 at the first Temp position and pressing Max Temp count per program 12 Using a Temp count of 7 or more requires as much memory as two 6 Temp programs Segments Temp count can be added or deleted by selecting Insert Delete from the pop up menu while the cursor is positioned anywhere except at the start of a block Using Extension Mode Extension mode provides the following functions but model TP650 does not have the Gradient Function Extension Settings Temperature and time settings must be increased decreased each time cycle count is increased Temperature extension setting is changed by enabling Extension mode moving the cursor to the TempEX position and input a value Go to MENU select and press ENTER Time extension
87. llowing Slope screen a gene wenu Cancel Ti 10 00 60 00 15 00 E 0 00 0 00 0 00 Slope Setting Range Temperature 30 0 to 99 9 C Time 1 30 1 minute 30 seconds to 99 59 99 minutes 59 seconds Note The Minimum Time Setting for Slope is 1 min and 30 s Any setting shorter than the minimum setting will automatically be adjusted to this value INote The Slope setting is canceled by moving the cursor to the designated position and pressing the key or going to MENU selecting Slope and pressing again These steps reset the current slope setting to its default value 0 0 4 Gradient Function Settings only for TP600 With the cursor positioned on Temp or Time go to MENU select Grad and press ENTER to select a setting It becomes Gradient temperature setting screen i1Temp i3Temp 25cyc 2 Te MENU1 U Save U Insert 72 QDelete Grad U Cancel D TD3010JE_v3 00D 83 TP600 TP650 Operation Manual It becomes Gradient temperature setting screen Gradient Setting 02 46 0 03 47 0 04 49 0 05 51 4 06 53 8 07 56 2 08 58 6 09 61 0 10 63 0 11 64 0 65 0 the low temperature setting at position 01 The left end of a block and the high temperature setting at position 12 The right end of a
88. n 05 02 14 12 00 00 25 0 GTakaRa 00 OO 0 0000 00000 000 0 0 6 0 0 0 0000 06 O O 00 00 00 00 TaKaRa OO 00000 0 0 0 00 00000 Uu 0 0 0 0000 00 0 0 0 00000 OO 00000 00000 HHHHHHHHHHHHHHHHHHHHHHHH LHHHHHTHIHHIHHHUHHHUHHHIHHHTITIHIHIHIHITIIHHHHLHIHHHTHHL Dun ul Du 000000000 00 000 0000000 ENTERO 0 000000 TaKaRa Thermal Cycler Dice MENU TP600 Ver 3 xx RUN User Common 05 02 14 12 00 00 25 0 C EE S EE 000 HHHHHHHUHUHHHHHHHHHHHHHHENTERIIHHHHIMENUHTT LEO 00000000000 0000 ENIERHHHHHHHHH A Program Name Stored MENU MENU RT PCR 05 04 01 Start Cycle seq 05 04 02 TaKaRa 05 04 02 Touch Down PCR 05 04 02 Sort TD3010JE_v3 00D TP600 TP650 NOTE HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 0 0 MENU HHHHHHHHHHHHHHHHHHHHHHHHHHHHH 0000000 0 0000000 NOTED 000 00 0000 0 0 000 pnnpmmnmmmmmnnmilc 00000000000 0 0 OEnter 00 1 25 i2Temp 1 Cancel Date of stored Date of last used HHHHHHHHHHHHHL IProgamname 00000000 0 00 000 Date of stored 10000 Date of last used O 0000000 00 OKU Enter 0 TD3010JE_v3 00D TP600 TP650 O0 0000 00 0 0000 0 0 000 0 Select Reaction Mode MENU OK Mode 1 1 5 Container 1 1 Thin 2 Thick Volume 50ul 5 150 HI 000000000000000000
89. n to which the pop up menu is jumped over with the ENTER key and the key is possible System Operational Flow The basic flow of system operation is shown below 1 Settings function Selection of date time and display format Section 2 Chapter 5 2 Program design User selection and registration Section 7 Chapter 4 Program creating or editing Sections 5 and 6 Chapter 4 Program link function Section 8 Chapter 4 3 Launching program Operating program procedure Sections 2 and 3 Chapter 4 4 Terminating or Aborting system operation Section 4 Chapter 4 5 Maintenance and System tools For description of inspection diagnosis backup and deletion procedures Sections 1 4 3 and 5 Chapter 5 Key Entry System Notes that alphanumeric keys are assigned multiple characters selectable by repeatedly pressing the key to cycle through the options Okey amp 0 1 key space 1 2key a b c 2 key D d e f 4key GH I g h 1 4 5 J K k l 5 6 key 6 7 5 4 7 okeyl T V t u 8 9 W X Y Z w x z 9 TD3010JE_v3 00D 67 TP600 TP650 Operation Manual 2 Turning On the Power The TP600 TP650 uses a 100V AC to 240V AC non switched 50 60 Hz power line Electric power is 490 VA and rated current is 4 9A when operating at 100 V Note Be sure to check local powe
90. nds Pause setting by moving cursor to TimeEx of the target segment select Pause in pop up up menu and press ENTERI The figure below shows an example of a program setting procedure using 55 C and 30 s as the temperature and time settings with Time setting decremented by 5 s each cycle The Pause is set for activation when the 55 C segment is completed and operation moves to 72 C Procedure 1 Use the arrow keys to activate highlight the selected position 6 T Press MENU key to display pop up menu then use arrow key to select Pause Press ENTER key 2 Use numerical pad keys to 5 3 Press key to display pop up menu then use arrow key to select 4 Press key 5 Use the arrow keys to activate highlight the selected position TD3010JE_v3 00D 82 TP600 TP650 Operation Manual Note The extension setting can be canceled by pushing key or entering 0 and the Pause setting is cancelled by going to MENU and selecting Pause again Note Pop up menu content varies according to cursor position The pop up displays Save Insert and Delete when the cursor is positioned at Temp or Time Save Slope and Cancel are displayed when positioned at lempE Save Pause and Cancel when positioned at TimeEx TP650 TP600 MENU 1 Grad _ Cancel Cancel 3 Slope Function Settings With the cursor placed on TempEx go to MENU select Slope and press ENTER This displays the fo
91. ng Delete User Program With folder programs displayed use WA to select desired program then go to MENU choose Delete and press Enter Program Name Stored MENU MyApp6789012456 03 04 01 perete Cycle Seg 03 04 01 1 105194 101 Touch Down PCR 03 04 01 When the following confirmation message appears select from MENU and press Enter Confir nation OK Cancel Are you sure to delete Program Name TaKaRa 5 2 Using Delete User Name When the confirmation message appears select OK from MENU and press Enter Confir nation OKI Are you sure to delete 2 User Name Ichiro lt NOTE gt The Programs are exist message appears when programs are still present in the username file and the delete operation is terminated Making sure to delete the programs first 5 3 Using Delete All Program in User Confir nation Are you sure to delete All Program in Common Selecting OK from MENU and pressing Enter displays the flashing Seek and Erasing message Deletion is complete in about 15 seconds and the main screen is displayed TD3010JE v3 00D 106 TP600 TP650 Operation Manual Chapter 6 Troubleshooting Any errors that occur during system operation are logged in the system History The See History message is displayed under Result in the system closing screen The See History message is also displayed wh
92. ng Link Programs A single program name is assigned the symbol followed by single digit numbers at the end Example PLANT TESTZ PLANT TEST Programs numbered 1 to 4 PLANT TEST will be executed in that order PLANT TESTZ Note The Link function ignores all numbers except the first digit following the symbol Examplel LINKZ LINKZ Long Execution commences with LINKZ Short Program 1 and continues to 4 LINK I Note Avoid Program Names with Same First Digit Although possible to save programs to the system that have the same first digit after the symbol if they have differing subsequent digits they will be regarded as identical by the Link function They will be executed in the order of their system memory addresses rather than when created or how named resulting in confusion Order of execution depends on location in memory Example Program Names LINK LINK pee LINK LINK Long ANNAL LINK Short LINK Short LINK Long LINK Short TD3010JE_v3 00 92 TP600 TP650 Operation Manual Link Function Program Execution Procedure 1 from program username screen to main screen and press MENU Then select Run from pop up menu and press ENTER Example Program Created for Username Common Select User Name lt TaKaRa gt Sabro In MENU select OK and press ENTER TaKaRa Thermal Cycler Dice mini TP100 Ver x xx User Common 05
93. o be deleted From the main screen go to MENU select User and press Enter Select the user name to be deleted and then go to MENU choose OK and press Enter to move to the desired folder The User folder in this example is named Commons 2 Use W to select Tools from MENU and press Enter to display the Select Tools Screen TaKaRa Thermal Cycler Dice TP600 Ver X XX User Common Tools 03 01 15 12 57 31 3 In Select Tools select Delete User Data choose OK from MENU and press Enter to move to the Delete User Data screen Select Tools History Setting Svc PC Communication Cancel Diagnosis Reserved Delete User Data 4 Select the desired function from the Delete User Data screen Delete User Data MENU ELI Delete a User Program Delete a Use Name Delete All Program in User Delete User Data provides the following functions 1 Delete User Program Deletes an individual program in the user folder 2 Delete User Name Deletes a user name Note that the Programs exist message appears when there are programs remaining in the user folder All programs in the user folder must be deleted before deleting a user name TD3010JE v3 00D 105 TP600 TP650 Operation Manual 3 Delete All Program in User Deletes all programs in the user folder without deleting the user folder user name itself Use the Delete User function to delete a user name 5 1 Usi
94. p programs Notel When the pop up menu is MENU2 it is possible to switch to MENUT by pushing a right and left arrow key Using Extension Mode Extension mode provides the following functions But model TP650 does not have the Gradient Function Extension Settings Temperature and time settings must be increased decreased each time cycle count is increased Temperature extension setting is changed by enabling Extension mode moving the cursor to the TempEX position and input a value Go to MENU select and press ENTER Time extension setting is changed by moving the cursor to the TimeEX position and input a value Go to MENU select and press ENTER e Pause Setting TD3010JE_v3 00D 75 TP600 TP650 Operation Manual With Pause activated the program pauses when operation moves to the selected pause segment Program operation is resumed by going to MENU selecting Resume and pressing Note Pause is not designed to activate after the selected segment has been executed With the Extension mode enabled go to MENU select Pause and press ENTER to activate Pause To cancel pause go to MENU select Pause and press again Slope Setting This is used to set the desired temperature heating cooling speed setting Do this by setting the desired target temperature and ramp time settings The resulting temperature change slope will then be used to perform linear temperature control Move cur
95. peration 4 Fast speed turning for disregarding overshoot temperature 5 Enables system operation with high temperature control Semi Compatible with TaKaRa PCR Thermal Cycler PP normal mode operation Mode performance outlook Ramp Speed Hi 5 gt 4 gt 1 gt 2 gt 3 Low Container 1 Tube 2 Plate Select desired container TaKaRa PCR Tube of 0 2ml Hi Tube with Dome Cap Code NJ200 is a thin wall type tube Select 1 when using this tube Volume 5 150 micro litter Select reaction volume Usually below 100micro litter INote The above information settings are saved separately for each program When the program is started each setting condition is memorized Select OK from the pop up menu and press ENTER 00 This displays the program pattern screens shown below Note that the patterns show a program that has not yet started Extension Data Not Selected TaKaRa Taq Remaining 1 27 Start Cancel Extension data shown here TD3010JE_v3 00D 71 TP600 TP650 Operation Manual Notel The Extension mode allows the user to temperature time increments decrements pause and gradient settings Notel Program names are registered using up to 16 characters however the pattern screens can only display 12 characters Go to the pop up menu select Start and press ENTER This starts the program Program Operating Screen TaKaRa Taq Remaining 1 22 Pause info Changing temp
96. quipment do not exceed the rated power supply capacity of the common power source The system body and power supply cable are subject to high voltage levels The maximum voltage is a line voltage AC100V 240V Avoid inserting metal or flammable objects inside the system or handling it with wet hands as this can result in electric shock and or other dangers CAUTION When moving the system be sure to first disconnect the power cable as well as any peripheral devices Be sure to disconnect the power cable prior to cleaning or servicing the system Other precautions Observe the following precautions in order to prevent electric shocks and fires Avoid inserting metal objects flammable materials or other foreign WARNING objects into the various openings on the system case Never remove the system cover or attempt to alter it in anyway Avoid disassembling or making any direct contact with internal parts of the system Should noxious fumes smoke or other abnormal signs appear immediately terminate operation turn off system power and disconnect the power cable Wait until conditions stabilize before contacting the nearest sales agent or the company technical support line Should water or other foreign matter enter the system immediately terminate operation turn off system power and disconnect the power cable Wait until conditions stabilize before contacting the nearest sales agent or the company t
97. r cable provided with the unit 12 Air Vent rear Openings used to cool the interior of the unit lt Caution gt Do not to block any of the air vents 13 Brightness Adjust Knob Used to adjust display brightness 14 Communications Port Connector RS232C connector 9Pin used to link unit to computer NOTE The RS232C cable not included as standard accessory TD3010JE v3 00D 65 TP600 TP650 Operation Manual Key Pad MENU Key Power L ED Alphanumeric Switches pop up menus on off Lights up when the Wakes up display from LCD power is on sleep mode rA TaKaRa FCR Thermal Oy RSN DOG 2 a 4 N9 NO pos WWZ 7 9 Arrow Keys Used to move cursor and STOP Ke Key move between pages Used to abortor Used to clear Used to fix any entered stop system any entered information and operations information selecting functions Lid Section Lid Lid Handle Lid Heater TD3010JE v3 00D 66 TP600 TP650 Operation Manual Chapter 4 System Operation 1 Introduction This system operation explains both TP600 and TP650 Gradient Function is only for TP600 Operation Based on Pop up Menu Configuration Operation begins by pressing the MENU key which displays the pop up menu screens Menu items are highlighted using the arrow keys and selected by pressing the ENTER key Pop up menus can be closed by simply pressing the MENU key A part of operatio
98. r line specifications prior to connecting the system Notel Make sure that the power plug is inserted snugly into the power outlet Turn on the power switch The beeper sounds twice and the select User Name screen appear Using arrow keys select the User Name and push the MENU key lt TaKaRa gt Sabro Cancel INote When the pop up menu is active the arrow key does not function The following password prompt screen appears if a password has been registered for the selected user Note that Common and TaKaRa have been pre registered default user names that have not been assigned passwords User Jiro Password Note The above screen appears only when the username has been assigned a password If not the screen is skipped and the main system screen appears Note When forget password select Cancel from the MENU It is possible to go to the main system screen of 2 3 as user After input the correct password displays the main system screen shown below All system operation begins with this screen TaKaRa Thermal Cycler Dice TP600 Ver 3 xx User 05 02 14 12 00 00 25 0 C TD3010JE_v3 00D 68 TP600 TP650 Operation Manual Notel Users who do not possess a password or have forgotten theirs may simply go to MENU select Cancel and press ENTER This logs the user onto the system as Common user and displ
99. row keys to move cursor Press or an arrow key after input numerical values of temperature Temp time time and cycle count Cyc Temperature using numerals For example for94 0 C 940 Temperature setting range 4 0 to 99 9 C Time using numerals For example for 1 minute and 30 seconds 0130 Time setting range 00 01 s to 99 59 99 minutes 59 seconds when operating in continuous 9999 Cycle count 1 to 99 TD3010JE_v3 00D 14 TP600 TP650 Operation Manual Procedure for Inserting Deleting Patterns and Segments Patterns can be inserted or deleted by placing the cursor on the pattern start position Example placing cursor 1 94 0 or 10 00 of 1 Temp 3 94 0 or 0 30 of 3 Temp and selecting Insert Delete from the pop up menu Save Segments can be inserted or deleted by placing cursor anywhere except the pattern start position Example placing cursor on 55 0 0 30 or 72 0 1 00 for 3 Temp 25 on the screen below and selecting Insert Delete from the pop up menu Run Cancel Temp 94 0 3 arrow gt Time 10 00 Ov Switch Segment Temp count in pattern is modified by inputting a new value 1 to 9 in the first Temp position in pattern and pressing Max segment Temp count per program 12 INote Using a segment Temp count of 7 or more requires the double memory capacity as two 6 segment Tem
100. s for amplifying the test target TP600 Main Feature The newly developed PCR Thermal Cycler Dice TP600 system is equipped with a gradient function that enables the user to set a maximum 20 C temperature gradient between the left and right rows in the sample block at each temperature condition setting step This has made it possible to optimize the reaction conditions for a single test and thereby design a new high performance functionally enhanced system at a lower cost lt Enhanced Functionality and Ease of Operation gt Gradient function enables consideration of annealing temperatures TP600 TP650 Features The control panel and display units are integrated in a variable angle wing panel configuration enabling the creation of a more compact and stylish system that can be installed almost anywhere lt Enhanced Functionality and Ease of Operation gt e Easy to operate slide and lever type lid e New variable angle control panel enhances visibility and ease of operation e Graphic display makes programming the system much easier Simpler operation through combination of pop up menus and mobile phone type alphanumeric character input pad Extended time functions ideally suited to long PCR Extended Temp function that makes touchdown PCR possible Slop function Dice version 2 and up Link function that chain any User Programs and run Dice version 3 8 types of preset protocol that make starting PCR easier Modes tha
101. setting is changed by moving the cursor to the TimeEX position and input a value Go to MENU select and press ENTER Pause setting With Pause activated the program pauses when operation moves to the selected pause segment Program operation is resumed by going to MENU selecting Resume and pressing Note Pause is not designed to activate after the selected segment has been executed With the Extension mode enabled go to MENU select Pause and press ENTER to activate Pause To cancel pause go to MENU select Pause and press again This is used to set the desired temperature heating cooling speed setting Do this by setting the desired target temperature and ramp time settings The resulting temperature change slope will then be used to perform linear temperature control Move cursor to TempEX go to MENU select Slope and press ENTER TP600 only With the cursor positioned on Temp or Time go to MENU select Grad and press TD3010JE_v3 00D 81 TP600 TP650 Operation Manual Enter to select a setting 1 Enable Extension mode by selecting More from pop up menu and pressing 2 ERE ET Cancel 0 30 0 00 BHE Pause Cancel U U UU Position cursor at the Ex line below the Temp or Time lines desired value and press ENTER Then select increase decrease or Pause from pop up menu and press again Max temp Ex value 9 9 Max time Ex value 9 59 9 minutes 59 seco
102. sor to TempEX go to MENU select Slope and press ENTER TP600 only With the cursor positioned on Temp or Time go to MENU select Grad and press Enter to select a setting 1 Enable Extension mode by selecting More from pop up menu and pressing 2 Cancel U U UU Position cursor at the Ex line below the Temp or Time lines desired value and press ENTER Then select increase decrease or Pause from pop up menu and press again Max temp Ex value 9 9 C Max time Ex value 9 59 9 minutes 59 seconds Pause setting by moving cursor to TimeEx of the target segment select Pause in pop up up menu and press ENTER The figure below shows an example of a program setting procedure using 55 C and 30 as the temperature and time settings with Time setting decremented by 5 s each cycle The Pause is set for activation when the 55 C segment is completed and operation moves TD3010JE v3 00D 76 TP600 TP650 Operation Manual to 72 C Procedure 1 Use the arrow keys to activate highlight the selected position 2 Use numerical pad keys to 5 3 Press MENU key to display pop up menu then use arrow key to select 4 Press ENTER key 5 Use the arrow keys to activate highlight the selected position 6 Press MENU key to display pop up menu then use arrow key to select Pause 7 Press key i1Temp 3Temp 25cyc 2Tem MENU 1 Notel The
103. t OK in MENU and press ENTER Mode 1 1 3 Container 1 1 Thin 2 Thick Volume 150 ul Link 2 2 Selecting 2 No for Link cancels the Link function 3 Going to MENU selecting Start and pressing ENTER executes the program until it is finished Note Display on running with Link function 1 Program Execution Screen Program Title displays name of program in progress Remaining Time indicates time remaining for execution of current program and Info displays the starting time and expected finishing time of current program in progress 2 Finished Program Screen The Finished screen appears program name program execution starting and completion time and length of run time of the only last program 3 Program History Screen Finished program name is comprised of the name before the and starting program number finishing program number TD3010JE v3 00 94 TP600 TP650 Operation Manual Example LINK 1 LINK 2 Long LINK 3 Short LINK 4 When the Link programs finished History screen registered a program name as LINK 1 4 User lt Commons Program LINK 1 4 Mode 1 In 50 ul Started at 11 50 10 05 01 21 Finlshed at 15 28 15 05 01 21 Notelllo view the program history go to the main system screen select Tools from the pop up MENU and press ENTER Next select History then choose OK in the MENU and press ENTER Refer to section 1 Saving History Started at ind
104. t ensure compatibility temperature rise characteristics with earlier Takara Thermal Cycler models Air intake vents on the front and sides and exhaust vents on the rear that reduce heat based interference with other equipment lt Ease of Maintenance gt System firmware updates and data backup can be performed easily through connection to personal computer Automatic creation of operating history and error logs TD3010JE_V3 00 60 TP600 TP650 Operation Manual Chapter 2 Installation Requirements and Maintenance 1 Installation Conditions To ensure optimum performance and long and stable product life observe the following precautions when selecting an installation location Note that installing the system in a location that does not meet the requirements listed below can result in malfunctions and or system breakdown Replacement of components and system repairs for any deterioration in performance or damage caused by failure to adhere to the following conditions will not be covered by warranty 1 Install system in a comparatively dust free location 2 Avoid locations subject to corrosive or flammable gases 3 Avoid locations subject to either strong shocks or continuing low levels of vibration 4 Install the system away from direct sunlight 5 Install the system as far away as possible from electrical equipment that generates strong magnetic fields electrical fields and high frequencies Avoid locations near eq
105. tHHHHHEnterlHHHHHHLH 00000 000 00 00 TD3010JE_v3 00D TP600 TP650 HHHHHHLH HHHUHHHHL D D UD LI UI UU UU HHHH 00000 0 0 00701 00 0 00000 HHHHHUHHHUHHHHHHHHL Lee L DU Ducum 0 0 00 000000000 00000 00 000 0000 00 0 StatQ 0 0 00000 000 OOOO DL LE LIL Lid is warming up x x C 10HEL 000 E OOOO 000000 08 00000000000 0 0 0 10000 1 25 Remaining 1 22 1 25 Remaining 1 22 Program Informatlon User Common Program TaKaRa_Taq Mode 1 Thin 50 Ll Run Started at 09 47 12 05 02 20 Run will end at 09 54 32 05 02 20 0000 00 00000000000 OKU EnterQ 00 TD3010JE_v3 00D TP600 TP650 OO 0000000 Stop 000000 00 0000000000000 Confirmation Are ou sure to stop A A P Temperature 54 6 C 00 000000000000 EnterQ 00 0000 00 0 0000 Finished TaKaRa Taq User Common Started at 14 14 23 03 01 15 Finished at 14 33 41 03 01 15 Length of Run 00 19 18 Over 0 0 C UnderO 3 C Result St opped HOU HUDDUD H UOUUWWWH HHHHHHHHHHHHHHHHUHHHHUHHHHHHHHHH UU 00 00000000000 OKU Enter 000000000000000 TD3010JE_v3 00D TP600 TP650 OO 00000 0000 _ 00 0000 000
106. te Remaining 0 07 Cancel Gradient items are displayed in the menu during gradient control enabling monitoring of the temperatures in each row After the timer has counted for 2 or 3 min during the gradient temperature program selecting OK for Grad from the pop up menu displays the following monitoring screen This will not be saved Gradient Temperature 01 4918 02 50 03 50 04 51 8 05 53 06 54 07 55 4 08 56 09 57 10 58 9 11 59 o Results are displayed the center of the sample block TD3010JE v3 00D 103 TP600 TP650 Operation Manual Result of Grad Test Heat 3 1 C s Cool 2 0 C s Over 0 260 5 Started at 16 21 42 03 01 15 Finised at 16 31 05 03 01 15 Length of Run 00 09 23 Resul t Nor mal Reference value lt 1 C by 2 to 3 minutes after Timer begins Select OK from pop up screen and press ENTER key to return to the main system screen TD3010JE_v3 00D 104 TP600 TP650 Operation Manual 5 Delete User Data Function This function deletes a username a user program and all programs in a Use folder Users can now delete all programs in the User folder with one stroke instead of having to go to the folder and delete each program separately Note that the User folder user name is not deleted A User name can be deleted using the Delete User function Procedure 1 Go to the User folder t
107. tems does not guarantee the performance of this instrument lt Outside US gt Purchase of this instrument conveys a limited non transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under one or more of U S Patents No 5 038 852 5 656 493 5 333 675 5 475 610 and 6 703 236 claims 1 6 only or corresponding claims in their non U S counterparts owned by Applera Corporation Noright is conveyed expressly by implication or by estoppel under any other patent claim Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA Applied Biosystems does not guarantee the performance of this instrument TaKaRa 0 00000000000 UUUOUUUUOUUUUUUUUUUUOUUUUUUUOWUUUWUUUU LIHIHIHIHHHHHHHHHHHHHHHHHHHHHUHHHHHHHHHHHL LEO TaKaRa HL E 00 0 0 00000000000 00000 0000000 Ug i i DD L 00 0 0 00 0 00 0 000 0 00 0000 0 000 0 0 0000 HH Up 00 0000 00000010000000010000000000000000 HHHHHHHHHHHHHHHHHHHUHHHHUHHHHHHHHHHHHUHHHHHLH 0 0 0 00000 0 0000 0 00000 000 000 0000 TEL 03 3271 8553 03 3271 7282 Lj 103 8232 0100000000210 1510 000000 TEL 077 543 72971 077 543 7293 920 2193 0000 000 00040 1 HHHH TEL 077 567 9261 FAX 077 565 6995 l
108. tion select View from the pop up menu and press ENTER TD3010JE_v3 00D 69 TP600 TP650 Operation Manual 2 1Cyc i1Tempi3Temp 25 2 Sorting Program Names To change program name order select Sort and press ENTER Select Sort Date stored Date of last used Select from the following sort options Program name sorts alphabetically by name in ASCII code Date saved sorts in order of date saved Date last used sorts in order of date used Then go to pop up menu select OK and ENTER key QO Select or directly Reaction mode rur Container 1 1 Thin 2 Thick Volume 50ul 5 150 HI When the program that adds the link function is selected it becomes the following displays A m Container 1 1 Thin 2 Thick Volume 50ul 5 150 HI Link 1 1 Yes 2 No Link Select 1 or 2 for Link function 1 Yes When operating the program by using the link function 2 When operating only one program Please refer to the paragraph of the link function for details TD3010JE v3 00D 70 TP600 TP650 Operation Manual Mode 1 Enables system operation with maximum temperature control Recommended for normal operation 2 Enables system operation with high temperature control Compatible with TaKaRa PCR Thermal Cycler SP operation 3 Enables temperature control compatible with linked compressor unit Compatible with TaKaRa PCR Thermal Cycler MP o
109. uipment that generate noise caused by electrical motors and transformers 6 Choose a flat location capable of withstanding the design weight of the system 7 The installation environment must be within 15 to 30 C temperature range and a 20 to 80 relative humidity range 8 Avoid locations subject to sharp temperature fluctuations exhaust from air conditioning systems and condensation caused by sharp humidity fluctuations 9 Avoid blocking air vents located on the right and left sides front and rear of the system 10 Choose an operating location with good ventilation 11 Do not operate the system in a cold room 12 Select a room that can provide sufficient power frequency voltage and current for system operation 13 Do not attempt to move the system while holding it by the lid handle or the control panel as this can damage to key components Doing so can also result in personal injury due to part breakage resulting in the system falling down lt Environmental Conditions gt This equipment is designed to be safe under the following conditions based on the OO ces TD3010JE_V3 00 IEC 61010 1 Indoor use Altitude up to 2000 m Ambient temperature Operating environment 15 C to 35 Humidity range 20 to 80 relative humidity Supply voltage fluctuations not to exceed 10 of the nominal voltage Other supply voltage fluctuations as stated by the manufacturer 61 TP600 TP650 Operation Manual
110. utt D D p O 000000 010 0000000000000 0000000 1000 000 0 0 0 0 000 OL 00 C 0 00 000 00 A 00 0 0 0 0 0 00 60 0 000 LINOTEH D 000 0000 00 0 06 0 0 0 U 00 000000000 O UU D E 00 00 L 000 D D 0 11 D 00 0 0000000 LI HHHHHUHHHHHHHUHHHHHHHHHH H 000000 00 HHHHHHHHHHHHHHHHHHHHHHHHH 00000000000 00 0 0 0 0000 00 0 EXTI 0000000 uguali 00000 00 0 j D L DL DELELELELELELEIILE 000 00 0 00000000000 0 00 0 TD3010JE_v3 00D TP600 TP650 DOO 0 0 0000 Pause DD 0000000000 00000000000 N0 00 000 0 00000000 LE EL E E E Resun Enter 000000000000000000000000000000 00000000000 Time Exp 0 0 0 000 Pause ENTER Pause 0 DO O MENU Pause ENTER 1 0 0000000000 0726500 0 00 00 0000 0 000 00000000000 0000 Timo 0 00000 MENU Grad ENTER L 0 000000 00000000000000000000000000000000 000000000000000000000000000000 0000 000000000000000 00000 Temp 0 D 0000 DIMENU ENTER 0 0 0 0 00 o 000000000 0000000000 0 0 Morel EnterQ Temp 94 0 KENN 55 0 Time 10 00 0 30 0 30 Time 10 00 0 30 0 30 1 Ex 0 00 0 00 Pause 0000000 erpil 0 Tire D 0 DEXTI 00000000000 Enter OO
111. vrir en cours de fonctionnement Description of Danger Never disassemble system as this may lead to electrical short circuits leakage current and or fire The case and power cable are subject to high voltage levels so take care to avoid electric shocks Always ground the system to prevent electric shock from leakage current Avoid touching the heat cover which reaches very high temperatures and may cause burns Be sure not to block air vents when installing the system as this can cause internal overheating Be sure to close the cover while the system is in operation Failure to do so can lead to burns and or damage to the eyes A This symbol means caution or warning S This symbol means an action is prohibited This symbol means an instruction must be followed AL Electrical Symbols The symbols shown on this page are adapted to the TaKaRa PCR Thermal Cycler Dice Read the explanations and make sure you understand what the symbols mean before you interact with the instrument in any way This symbol indicates the on position of the main power switch O This symbol indicates the off position of the main power switch Precautions against Transferring or Copying Content of This Document The content of this document cannot be transferred or copied without the express permission of the copyright holder Copyright holder TAKARA BIO INC Printed in Japan Contact TAKARA BIO INC TAKARA B

TaKaRa PCR Thermal Cycler DiceTM Operation Manual 取扱説明書

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