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Electrophoresis Power Supply EPS 3501

1. HSI19N3 The parameter that should be chosen as the constant and thus control the electrophoresis depends on the type of electrophoresis In the case of homogeneous buffers throughout the system same electrode and gel buffer the conductivity is constant during the electrophoresis If the conductivity is constant the voltage will be proportional to the current and the power to the square of the current according to 1 and 2 This means that the result of the electrophoresis will be the same regardless which parameter is chosen for constant For historical and practical reasons voltage is most commonly used for regulation Submarine gel electrophoresis of DNA RNA and pulsed field electrophoresis are usually run at constant voltage SDS PAGE using continuous buffer systems is run at constant voltage or current For discontinuous buffer systems the resistance will increase as the electrophoresis proceeds due to a decrease in conductivity Running at constant voltage will result in decreasing current and power Constant voltage will thus be safe in the respect that the power will not increase and produce more and more heat On the other hand the separation will slow down and impair resolution due to an increased time available for diffusion Running at constant power would give a faster electrophoresis and controlled power while running at constant current would at the first sight seem to be problematic because of increasing volt
2. 1 Selezionare il numero del programma Sar P1 OFF 3000V 50mA 20W 8 W 2 Avviare le operazioni SET P2 3000 Vh 2500V 50mA 30W Selezionare con Avviare con RUN PZA 1Vh 500 6 MA 30W RUN Pra 500 Vh 1200V 25mA 25W q attendere 5 secondi SEI P2 3000 Vh 2500V 50mA 30W PAUSE p2 500 Vh 1200V 25mA 30W 3 Durante il funzionamento possibile visualizzare le impostazioni E anche PSE Fas 3000 h possibile interrompere il 2500V 50mA 30W funzionamento e modificare il programma Programmazione 4 Interrompere il funzionamento RUN p2A 2500 Vh manualmente 2500V 10mA 25W END p2 2500 Vh 2500V 50mA 25W 5 Il funzionamento viene interrotto END p2A 3000 Vh automaticamente 2500V 9mA 20W Fig 5 Riepilogo di funzionamento e funzioni di pausa passo dopo passo gt 5 Z Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD 13 5 Funzionamento fe Z lt lt 14 Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD WWW gehealthcare com GE imagination at work and GE monogram are trademarks of General Electric Company All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory GE Healthcar
3. Check the electrophoresis unit Check voltage selector Check mains voltage Press PAUSE CONTINUE to continue running a program or STOP to interrupt the run FAIL Code No xxx Call service f a serious error occurs the program enters the FAIL mode The output is switched off and an error message is shown in the display Please read the error code number and contact your GE Healthcare representative Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 25 HSI19N3 ENGLISH 7 Trouble shooting 26 Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 8 Technical specifications Technical specifications 8 Regulation Output range Programming range Output resolution Programming resolution Accuracy Line regulation Load regulation Ripple Short term stability Long term stability Start current check Sudden load change check Ground leakage check Output protection Recovery after power failure Ambient operating temperature Ambient operating humidity Ambient operating pressure Mains requirements Power consumption Dimensions WxDxH Weig
4. AuBerdem kann der Benutzer durch Dr cken der Taste SET nderungen im Programm w hrend eines Programmlaufs vornehmen wenn zun chst die Taste PAUSE CONTINUE gedr ckt wurde enter set V Change up Change down Mit dieser Taste werden Parameter Werte oder eine andere Option in dem blinkenden Feld ge ndert Numerische Werte laufen schneller ab wenn eine Taste gedr ckt gehalten wird durch Anklicken einer Taste ndert sich der Wert in voreingestellten Stufen Parameter oder Einheiten z B Vh und VES NOOptionen werden durch einmaliges Dr cken einer Taste ge ndert Die Tasten k nnen auch zum Umschalten zwischen Zeit und Voltstunden in RUN PAUSE und END verwendet werden Die Werte rollen d h sie laufen automatisch von ihrem H chstwert bis zu ihrem Mindestwert bzw umgekehrt Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD 9 DEUTCH 4 Technische Beschreibung 10 pause continue STOP RUN Nach dem Dr cken der Taste RUN beginnt der Programmlauf und das Programm wird in den RUNModus geschaltet Auf der Anzeige erscheinen die Programmnummer und die aktuellen Werte f r Spannung Strom und Leistung Auch die abgelaufene Zeit bzw die Voltstunden werden angezeigt Das Umschalten zwischen Zeit und Voltstunden erfolgt mit Jj PAUSE CONTINUE Mit dieser Taste wird das Ger t in den PAUSE Modus geschaltet und die Spannung wird abgeschaltet Die Anzeige zeigt den Status des Programmla
5. El signo de admiraci n en un tri ngulo equil tero en el manual advierte al usuario sobre la presencia de instrucciones importantes de operaci n y mantenimiento del aparato CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual ATENCI N Este es un producto de Class A En un ambiente dom stico este producto puede causar interferencias radioel ctricas en cuyo caso se le podria requerir al usuario la adopci n de medidas adecuadas Recycling This symbol indicates that the waste of electrical R and electronic equipment must not be disposed as unsorted municipal waste and must be EE collected sep
6. Off Rupture Manuelle En bas gauche au centre et droite sont indiqu es respectivement la tension le courant et la puissance 4 1 2 Clavier Touche ENTER Entr e Une pression sur cette touche permet successivement d entrer une valeur de la valider si cette valeur est correcte et de faire avancer le programme au champ suivant Les valeurs correctes sont tension 35 3500 V courant 1 150 mA puissance 1 100 W dur e 0 01 500 heures nombre de volt heures 1 500 000 Vh enter SET Programmation D marrage Une pression sur cette touche met l instrument en mode SET qui est le mode programmation et le mode d marrage En mode RUN ex cution une pression sur SET affiche les param tres programm s pour l ex cution en cours De plus une pression sur la touche SET permet d effectuer des modifications sur le programme en cours d ex cution apr s avoir appuy sur la touche PAUSE CONTINUE Wt Change up Change down Modif Une pression sur l une de ces deux touches permet de modifier le param tre la valeur ou le choix oui non dans le champ qui clignote On modifie les valeurs num riques de mani re acc l r e en maintenant la pression sur la touche concern e Un pression rapide sur une de ces touches modifie la valeur par increments pr d termin s On modifie les param tres ou unit s par exemple Vh et les choix YES NO oui non avec une seule pression sur la touche en question On peut aussi utiliser ces touche
7. manuel et utilis dans le m me tat que lors de sa livraison par GE Healthcare AB l exception des modifications d crites dans ce manuel ATTENTION Ceci est produit de Class A susceptible de produire des interf rences radio au cours d une utilisation domestique Dans ce cas l utilisateur est pri de prendre les mesures n cessaires Recycling This symbol indicates that the waste of electrical R and electronic equipment must not be disposed as unsorted municipal waste and must be wmm collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Ce produit est conforme la directive 73 23 EEC LVD Basse tension par la norme harmonis e EN 61010 1 1993 A1 1992 Normes EMS EMC Electromagnetic Compatibilitu Ce produit est conforme la directive EMC 89 336 EEC par la norme harmonis e EN 50081 1 mission et EN 50082 1 immunit Table des mati res Table des mati res 1 Introduction 2 Renseignements concernant la s curit i Pella 5 2 2 Caract ristiques de s curit intrins ques ss 6 3 Reception et installation 4 Description technique 4 1 Panneau avant 4 1 1 cran d affichage 412 ClOVIeFzs eben iia 9 4 1 3 Prises connecteurs de sortie ttt ttt tentes 10 4 2 Pannequ Qrri amp re rennes 10 5 Utilisation 5 1 G n ralit s i 11 5 2 Instructions simplifi
8. 4 Sperren der Strompr fung WARNUNG Wenn die Funktion Start Current Check gesperrt ist dann ist es m glich da an dem Stromversorgungsger t selbst dann Hochspannung anliegt wenn es nicht an das Elektrophoreseger t angeschlossen ist 1 Programmnummer w hlen SET PI OFF 3000V 50mA 20W W hlen mit 2 Programmlauf starten SEE P2 3000 Vh 2500V 50mA 30W Starten mit RUN P2 1Vh 500 6mA 30 W RUN P2A 500 Vh 1200V 25mA 25W oder 5 s warten 3 Der Benutzer kann w hrend eines Programmlaufs die Einstellungen ablesen Er kann den Lauf auch p2A 3000 h unterbrechen und nderungen im 2500 V 50mA 30W Programm vornehmen SET P2 3000 Vh 2500V 50mA 30W PAUSE p2 500 Vh 1200V 25mA 30W Programmierung 4 Programmlauf manuell stoppen RUN p2A 2500 Vh 2500V 10mA 25W END PA 2500 Vh 2500V 50mA 25W END P2 3000 Vh 2500V 9mA 20W 5 Programmlauf wird automatisch gestoppt Abb 5 Schrittweise Zusammenfassung von Programmlauf und Pausefunktion Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD 13 5 Betrieb E e E o un uy 14 Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD Important user information Normas de seguridad Haga el favor de leer el manual completo para comprender perfectamente el uso del EPS 3501 iADVERTENCIA
9. ENTER If volthours or h time were chosen the display will flash for the break value Set the value with 4 and confirm with ENTER Choosing alarm When a breakpoint has been entered the following question is shown Alarm at End Y Select YES or NO with WR and confirm with ENTER If YES is selected a small A appears on the right of the program number i e 2 Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 15 ENGLISH 5 Operation 16 Choose Program SET P1 number 3000 V 50 mA Select by Confirm by 2 Set Voltage limit P2 150 mA Select by Confirm by 3 Set Current limit SET P2 OFF 2500V 150 mA 100W Select by Confirm by 4 Set Power limit SET P2 2500 V 50 MA Select by Confirm by 5 Choose Breakpoint unit h or Vh or no Breakpoint OFF SEIE 2500 V P2 50 mA Select by Confirm by 6 Set Breakpoint SET P2 2500 V 50mA Select by Breakpoint off 7 Choose Alarm gt VES or NO AlarmatEnd Y Select by 0 m Confirm by 8 Program ready SET p2A 3000 Vh 2500 V 50 mA 50 W Confirm by SEI 2500 V P2 50 mA Fig 3 Step by step summary of programming Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Operation 5 HSI19N3 Back to start position The program is now back to the start position in SET mode with the program number flashing It is possible to go back to this positio
10. GE Healthcare die mit einem CE Symbol gekennzeichnet sind angeschlossen order Es wird an andere Produkte angeschlossen die in diesem Handbuch emphohlen oder beschrieben werden und Es wird in jenem Zustand verwendet in dem es von der Frima GE Healthcare AB geliefert wurde die in diesem Handbuch beschrieben sind WARNUNG Dies ist ein Produkt der Klasse A In h uslicher Umgebung kann dieses Produkt Funkst rungen verursachen In diesem Fall sollte der Anwender entsprechende Massnahmen ergreifen Recycling This symbol indicates that the waste of electrical R and electronic equipment must not be disposed as unsorted municipal waste and must be mm collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Dieses Produkt entspricht den Anforderungen gem der Niederspannungsvorschriften LVD 73 23 EEC und zwar im Rahmen der angepa ten Norm EN 61010 1 1993 A1 1992 EMC Normen EMC Electromagnetic Compatibility Dieses Produkt entspricht den Anforderungen gem der EMC Vorschriften 89 336 EEC undzwar im Ramhen der angepa ten Norm EN 50081 1 Strahlungsabgabe und EN 50082 1 Unempfindichkeit Inhaltsverzeichnis Inhaltsverzeichnis 1 Einleitung 2 Sicherheitsangaben Zi SIGNER eM manco 5 2 2 Eingebaute SIcherheitsMaBNahMeEn tti 6 3 Auspacken und Installation 4 Technische Beschreibu
11. a key is held down Clicking a key changes the value in preset increments Parameters or units e g Vh and yes no choices are changed with one key push The keus can also be used to switch between time and volthours in RUN PAUSE and END The values scroll i e they automatically change from maximum to minimum value or vice versa RUN F Pressing RUN starts the run and puts the program into RUN mode The program number current values for voltage current and power are shown on the display The elapsed time or volthours are also displayed Switch between time and volthours with y Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 13 ENGLISH 4 Technical description pause continue STOP 14 PAUSE CONTINUE Puts the instrument in PAUSE mode and switches off the voltage The display shows the status of the run at the time the key was pressed PAUSE CONTINUE only operates in RUN mode Time and integration values are retained In the pause mode SET can be used to make changes in the program Return to RUN mode by pressing PAUSE CONTINUE or RUN STOP Stops the run and puts the instrument in END mode The voltage is switched off and the end parameters are displayed Switch between time and integrated voltage by pressing V A run cannot be continued after pressing STOP Continue by pressing RUN to run the same method again or pressing SET to choose another program program a new method or make changes in an
12. ambienti estremamente umidi oltre il 95 Evitare la condensazione consentendo all unit di ritornare gradualmente a temperatura ambiente dopo il trasporto da ambienti molto caldi o freddi Conservare lo strumento il pi asciutto e pulito possibile Pulirlo regolarmente con un panno morbido Assicurarsi che lo strumento sia completamente asciutto prima dell utilizzo In caso contrario disinserire la spina dalla rete di alimentazione finch lo strumento non asciutto Utilizzare esclusivamente cavi elettrici non danneggiati ed attrezzature specificate per la tensione utilizzata cavi ad alta tensione devono essere conformi alla norma IEC 1010 2 031 1993 Tutte le attrezzature per elettroforesi collegate all alta tensione devono essere conformi alla norma IEC 1010 1 1993 Notare che l uscita e collegata al telaio massa di riferimento gt E 5 ZE 2 Informazioni sulla sicurezza 2 2 Funzioni di sicurezza incorporate L EPS 3501 omologato in conformit alle norme di sicurezza elettriche IEC 61010 1 EN 61010 1 L alimentatore dotato di diverse funzioni di sicurezza incorporate Sul display vengono visualizzati anche i messaggi di errore 1 Perdita di messa a terra funzionale Se l alimentatore collegato ad un unit per elettroforesi dotata di dispersione di messa a terra l EPS 3500 rilever il difetto e disattiver la tensione 2 Controllo della corrente di avviamento Per assicurarsi che l unit
13. cable de energ a correctamente conectado a la tierra de la toma de corriente 3 Losrespiraderos laterales y posteriores del aparato deben mantenerse destapados para permitir una ventilaci n suficiente 4 No utilizar el aparato en condiciones de humedad extrema m s del 95 Evitar la condensaci n dejando que la unidad se equilibre a la temperatura ambiente al trasladarla desde un ambiente m s caliente 5 Mantener el aparato tan seco y limpio como sea posible Limpiarlo regularmente con un trapo suave h medo Dejar que se seque del todo antes de usarlo Si se moja desenchufarlo hasta que se seque 6 Utilizar solamente cables el ctricos intactos y equipos especificados para las tensiones a usar Los hilos de alta tensi n deben satisfacer los requisitos de la norma el ctrica IEC 1010 2 031 1993 Todos los equipos electrofor ticos que se conecten a la EPS 3501 deben satisfacer los requisitos de la norma el ctrica IEC 1010 1 1993 7 T ngase en cuenta que la salida se conecta en el chasis masa de referencia m gt z e r Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD 5 E e lt a 4 Lu 2 Seguridad 2 2 Funciones de seguridad incorporadas La fuente de alimentaci n EPS 3501 est homologada seg n la norma de seguridad el ctrica IEC 61010 1 EN 61010 1 Adem s incorpora varias funciones de seguridad y mensajes de error que se indican en la pantalla 1 F
14. conductivity The high voltage is also turned off in this case and an error message is shown This function can be disabled to perform certain applications see 5 4 Optional programming 3 Sudden load change detection This function prevents accidents under running conditions due to a break in the electrical circuit such as a bad connection to the electrophoresis unit The high voltage is turned off in such an event Error messages are also shown on the display for sudden fails Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Unpacking and installation 3 HSITDN3 3 Unpacking and installation Unpacking Check the contents against the packing list supplied Inspect for any damage that may have occurred during transit Report any damage immediately to your local GE Healthcare representative and to the transport company concerned Mains connection Select the appropriate voltage range 100 120 or 220 240 V see Fig 2 inside front cover WARNING If the power supply is connected to 220 240 V with the range set to 100 120 V the instrument can be severely damaged WARNING Only use mains cables delivered or approved by GE Healthcare Select the appropriate mains cable and connect one end to the mains socket on the EPS 3501 power supply see Fig 2 inside front cover and the other end to an AC grounded outlet Switch on the power Each time the instrument is turned on a self diagnost
15. existing method MORE You can disable the start current check that otherwise detects if the resistance is too high and switches off the power See Section 5 4 EXIT Stops the execution of an operation such as the entry of a value Only values units that have already been confirmed by ENTER are retained Returns the instrument to the mode that was left or the start position in SET 4 1 5 Output sockets There are two sets of output sockets to allow two electrophoresis units to be connected and run at the same time see Fig 1 See inside front cover The voltage output is 0 3500 V The negative output socket gives between 0 and 1750 V and the positive gives between 0 and 1750 V 4 2 Rear panel The rear panel is shown in Fig 2 See inside front cover On the rear panel there is 1 A mains switch Press in to switch on the power to the power supply Press O to switch off the power 2 Asocket for the mains cable 3 A switch for voltage range The left position corresponds to 100 120 V and the right to 220 240 V 4 Vent openings Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Operation 5 HSI19N3 5 Operation 5 1 Overview The operation of the EPS 3501 is described in the following sequence 1 Programming or editing a method 2 Running a method When the method is running it can be interrupted put in PAUSE mode to apply samples and or change the program During a run it is
16. fils lectriques compl tement intacts et que des quipements pr vus pour les tensions que vous utilisez Les fils haute tension doivent tre conformes la norme lectrique IEC 1010 2 031 1993 Tout quipement d lectrophor se branch l unit d alimentation lectrique doit tre conforme la norme lectrique IEC 1010 1 1993 7 Notez que la sortie est branch e la masse du ch ssis r f rence Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD 5 FRANCE 2 Renseignements concernant la s curit 2 2 Caract ristiques de s curit intrins ques La conformit de l EPS 3501 la norme de s curit lectrique IEC 61010 1 EN 61010 1 a t certifi e Cette unit d alimentation lectrique est aussi dot e de fonctions de s curit intrins ques Des messages d erreur sont affich s l cran 1 Functional earth leakage Fuite la terre fonctionnelle Lorsque l unit d alimentation lectrique est branch e sur une unit d lectrophor se qui a des fuites la terre l EPS 3501 d tecte cette d faillance et coupe imm diatement la haute tension 2 Start current check V rification du courant initial Pour s assurer que l unit d lectrophor se est branch e correctement l unit d alimentation lectrique v rifie basse tension de s curit inf rieure 40 V que la r sistance n est pas sup rieure une limite d termin e Si cette r sistance est t
17. funziona solamente nel modo RUN Vengono visualizzati i valori di tempo ed integrazione residui Nel modo PAUSE SET pu essere utilizzato per modificare il programma Per tornare al modo RUN premere PAUSE CONTINUE o RUN STOP Ferma il funzionamento e commuta lo strumento modo End La tensione viene disattivata e vengono visualizzati i parametri finali Dopo aver premuto STOP non possibile continuare il funzionamento STOP MORE E possibile disattivare il controllo della corrente di avviamento il cui compito rilevare se la resistenza troppo elevata e disattivare l alimentazione EXIT Interrompe l esecuzione di un operazione come l inserimento di un valore Vengono conservati solamente i valori unit confermati in precedenza Lo strumento ritorna nel modo nel quale era rimasto o nella posizione di avviamento nel modo SET 4 1 5 Prese di uscita Vi sono due gruppi di prese di uscita per il collegamento ed il funzionamento contemporaneo di due unit per elettroforesi vedere Fig 1 La tensione erogata 0 3500 V La presa di uscita negativa fornisce una tensione compresa tra 0 e 1750 V quella positiva tra O e 1750 V 4 2 Pannello posteriore La Fig 2 vedere prima di copertina mostra il pannello posteriore Sul pannello posteriore si trovano 1 Uninterruttore principale Premere I per accendere l alimentatore Premere O spegnere l alimentatore 2 Una presa per il cavo di rete 3 Un commutatore di tensione
18. installation Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD Description technique 4 4 Description technique 4 1 Panneau avant Le panneau avant comporte un cran d affichage alphanum rique un clavier avec 9 touches une diode lectroluminescente LED qui s allume lorsque l instrument est mis sous tension Sortie HT active et des connecteurs pour deux unit s d lectrophor se 4 1 1 cran d affichage Un cran d affichage 32 caract res alphanum riques vous permet de suivre les diff rentes phases de programmation affiche les valeurs actuelles des param tres pendant l ex cution d lectrophor se et les valeurs finales des param tres Il affiche aussi des questions et des messages d erreur L cran d affichage comporte une barrette sup rieure et une barrette inf rieure La Figure 1 voir verso de la page de couverture montre l cran initial lorsque l interrupteur d alimentation est mis en position Marche On Le mode dans le cas pr sent SET c d programmation est affich en haut gauche et le num ro du programme au centre de la barrette sup rieure Le num ro du programme indiqu est celui du programme pr c dent Le clignotement du chiffre en l occurrence 1 indique qu il est possible de le modifier en appuyant sur une des touches yy En haut droite s affiche le param tre de fin programm pour l op ration d lectrophor se Dans le cas pr sent
19. local de GE Healthcare y a la empresa transportista Conexi n a la red Seleccionar la tensi n apropiada 100 120 220 240 V la figura 2 ver interior de la cubierta PRECAUCI N El aparato puede sufrir aver as graves si se conecta a 220 240 V habiendo seleccionado la tensi n de 100 120 V Seleccionar el cable de red adecuado Conectar uno de sus extremos a la toma de la fuente de alimentaci n ver la figura 2 y el otro a un enchufe de corriente alterna conectado a tierra Conectar la corriente Cada vez que se conecta el aparato se ejecuta un test de diagn stico autom tico y si se detecta un error se visualiza un mensaje en la pantalla y suena una alarma Conexi n de la s unidad es electrofor tica s Conectar los cables de la unidad rojo con rojo y azul o negro con azul la figura 1 ver interior de la cubierta El cable rojo es el positivo y el azul o negro el negativo PRECAUCI N Utilizar solamente hilos el ctricos intactos y equipos homologados para la tensi n a emplear Con un mismo programa pueden operarse dos unidades electrofor ticas simult neamente Para ello debe doblarse la corriente limitadora y la potencia La tensi n es la misma independientemente del n mero de unidades m gt z e r 3 Desembalaje e instalaci n e E a un uy 8 Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD Descripci n t cnica 4
20. mite de potencia SET P2 OFF 2500 V 50 mA 100W Seleccionar con Confirmar con 5 Elegir la unidad del evento h Vh o anular la ruptura OFF SET 2500 V P2 50 mA Seleccionar con Confirmar con 6 Fijar el evento SEI P2 2500V 50mA Seleccionar con Evento desactivado Confirmar con 7 Elegir alarma s YES o no NO Alarma en final V Seleccionar con Confirmar con 8 i Programa listo SET pa 3000 Vh SEI p2 OFF 2500 V 50 mA 30 W 2500 V 50 mA 30 W Figura 3 Sumario paso a paso de la programaci n 12 Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD Operaci n 5 1 Introducir MORE m s SEE Pali 3000 Vh 2500 V 50 mA 30W Seleccionar MORE m s con 2 Seleccionar el programa MORE Seleccionar Seleccionar con Confirmar con 3 Elegir inhabilitaci n del control Control de corriente de arranque de corriente no VES no Seleccionar con 4 Vuelta a la modalidad SET SET p2A 3000 Vh preparar 2500V 50 mA 30 W Confirmar y salir con Figura 4 Inhabilitaci n del control de corriente jADVERTENCIA Si se inhabilita el control de la corriente de arranque la fuente de alimentaci n puede suministrar alta tensi n aunque no est conectada al equipo de electroforesis 1 Elegir el numero de programa SET P1 OFF 3000V 50mA 20W Seleccionar con 2 Iniciar el funcionamiento SET P2 3000 Vh 2
21. power During the early stage of the formation of the pH gradient it is important to limit the current Otherwise the gradient will be irregularly shaped phase The conductivity is not constant along the gel so it is important to also limit the voltage This means running at constant voltage for the last phase phase III to prevent local overheating For detailed information about parameter values always follow the gel supplier s recommendations Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 21 ENGLISH 5 Operation 22 5 6 Short instructions This section summarizes the main points covered earlier in this chapter Use it as a check list once you are familiar with the detailed programming and running instructions Refer also to the separateschematic operating guide included with the power supply We recommend you keep this schematic guide close by the instrument 1 Turn mains power ON The display should have the program number flashing 2 Press ENTER for the actual program number or use to Y select the program number desired Press ENTER 3 For each of the parameters Voltage Current and Power press Y until the value desired is reached Confirm by pressing ENTER after each one and the display will automatically move to the next parameter 4 Choose between manual break or automatic break For automatic break choose breakpoint units in either Volthours Vh or time h Select the correct u
22. 4 Descripci n t cnica 4 1 Panel delantero En el panel delantero hay una pantalla alfanum rica un teclado con 9 teclas de membrana un diodo luminiscente que se enciende cuando se aplica la tensi n HV on y tomas para dos unidades electrofor ticas 4 1 1 Pantalla La pantalla alfanum rica de 32 d gitos sirve de gu a en la programaci n visualiza los par metros durante la electroforesis y los par metros finales posteriores Adem s hace preguntas y visualiza mensajes de error La pantalla tiene dos renglones La figura 1 ver interior de la cubierta ilustra una pantalla en posici n de arranque con la corriente conectada La modalidad en este caso SET se indica a la izquierda del rengl n superior En el centro del mismo rengl n se indica el n mero del programa previamente introducido La cifra intermitente en este caso 1 indica que puede cambiarse con las teclas Jy Ala derecha del rengl n superior se indica el evento programado en este caso desconectado Las posiciones izquierda central y derecha del rengl n inferior indican la tensi n el amperaje y la potencia respectivamente 4 1 2 Teclado ENTER Introduce un valor lo fija si es correcto y mueve la programaci n al campo siguiente Los valores v lidos son tensi n 35 3 500 V amperaje 1 150 mA potencia 1 100 W tiempo 0 01 500 h volthoras 1 500 000 Vh SET Pone el equipo en la modalidad SET modo de programaci n y arranque Pulsando l
23. 500V 50mA 30W Iniciar con r RUN p2A 1Vh 500 6 mA 30 W RUN p2 500 Vh 1200V 25mA 25W SEE p2A 2500V 50 mA 3000 Vh 30W PAUSE p2 500 Vh 1200V 25mA 30W o esperar 3 Durante el funcionamiento pueden verse los ajustes El funcionamiento puede interrumpirse para efectuar modificaciones en el programa P SET p2 3000 h 2500 V 50mA 30W m D gt PA e Programaci n 4 Parar el funcionamiento manualmente en a RUN P2 2500 Vh 2500V 10mA 25W END p2 2500 Vh 2500V 50mA 25W 5 Elfuncionamiento se para Ai END P24 autom ticamente 2500V 9mA 3000 Vh 20 W Figura 5 Sumario paso a paso de un proceso con pausa Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD 13 5 Operaci n E e E o un uy 14 Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD Important user information Standard di sicurezza Leggere attentamente il manuale per comprendere completamente i uso dell EPS 3501 AVVERTENZA Il punto esclamativo all interno di un triangolo equilatero indica all operatore la presenza di importanti istruzioni di funzionamento e manutenzione nella documentazione allegata al prodotto A CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It
24. 501 automatically switches over the controlling parameter according to programmed limits and conductivity variations in the system Two electrophoresis units can be connected to the EPS 3501 and run with the same programmed method at one time Three programs can be saved Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 7 ENGLISH 1 Introduction Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Safety information 2 HSI19N3 2 Safety information 2 1 Safety precautions Extreme caution should be exercised in the operation of this instrument as it can develop sufficient voltage and current to produce a lethal shock To avoid any risk of injury the instrument should only be operated by properly trained personnel and always in accordance with the instructions provided Read this entire manual before using this power supply 1 This instrument is designed for indoor use only 2 Theinstrument must always be used with the protective earth lead of the power cord correctly grounded to earth at the mains outlet 3 To permit sufficient cooling ensure that the vents in the rear and sides of the instrument are not covered 4 Do not operate the instrument in extreme humidity above 95 Avoid condensation by letting the unit equilibrate to ambient temperature when taking the power supply from a colder to a warmer environment 5 Keep the instrument as dry and clean as possibl
25. 81 3 5331 9336 Fax 81 3 5331 9370 e Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 e Middle East amp Africa Tel 30 210 9600 687 Fax 30 210 9600 693 Netherlands Tel 0165 580 410 Fax 0165 580 401 Norway Tel 815 65 555 Fax 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 Russia other C I S amp N I S Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 South East Asia Tel 60 3 8024 2080 Fax 60 3 8024 2090 Spain Tel 93 594 49 50 Fax 93 594 49 55 e Sweden Tel 018 612 1900 Fax 018 612 1910 Switzerland Tel 0848 8028 12 Fax 0848 8028 13 UK Tel 0800 616928 Fax 0800 616927 e USA Tel 800 526 3593 Fax 877 295 8102 imagination at work E Elanders sterv la 2006 User Manual 18 1130 21 AD 02 2006
26. GE Healthcare Electrophoresis Power Supply EPS 3501 User Manual Manuel d Utilization Bedienungsanleitung Manual del usuario Manuale dell operatore Electrophoresis Power Supply EPS 3501 amersham pharmacia biotech Program o9 60 9 WARNING High Voltage N 0 0 E E Fig 1 The front panel of the EPS 301 Figure 1 Le panneau avant de l EPS 301 Abb 1 Die Fronttafel des EPS 301 Figura 1 Panel frontal de la EPS 301 Figura 1 Pannello anteriore dell EPS 301 Ve Voltage 100 120 220 240V AC O CO ONE ENT C gt C J 2 C C gt A bee servicing AD CO ol0 Olle C gt 5 0 C gt C gt 100 120 O 220 240 C D 2 Fig 2 The rear panel of the EPS 301 Figure 2 Le panneau arri re de l EPS 301 Abb 2 Die R ckwand des EPS 301 Figura 2 Panel posterior de la EPS 301 Figura 2 Pannello posteriore dell EPS 301 Important user information EMC Standards All users must read this entire manual to fully understand the safe use of EPS 3501 WARNING The WARNING sign highlights instructions that must N be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The Caution sign highlight
27. La posizione sinistra corrisponde a 100 120 V quella destra a 220 240 V 4 Bocchette e lt lt 10 Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD Funzionamento 5 5 Funzionamento 5 1 Vista generale La seguente sequenza illustra il funzionamento dell EPS 3501 1 Programmazione o editaggio di un metodo Vedere Fig 3 e 4 2 Esecuzione di un metodo Vedere Fig 5 5 2 Breviistruzioni Questa sezione riassume i punti principali per l utilizzo dell EPS 3501 Fare riferimento anche alla guida schematica di funzionamento allegata all alimentatore Si raccomanda di conservare questa guida schematica vicina allo strumento 1 Accendere l unita Il display visualizza il numero del programma 2 Premere ENTER per visualizzare il numero attuale del programma oppure utilizzare 4 per selezionare il numero del programma desiderato Premere ENTER 3 Per ognuno dei parametri di tensione corrente e potenza premere yy fino a raggiungere il valore desiderato Confermare premendo ENTER dopo ogni impostazione il display passer automaticamente al parametro successivo 4 Selezionare tra interruzione del funzionamento manuale o automatica Per l interruzione automatica selezionare il punto di interruzione in Voltore Vh o tempo h Selezionare l unit corretta o per l interruzione manuale selezionare OFF con V T Confermare premendo ENTER 5 Se stato scelto voltore o t
28. a tecla SET en la modalidad RUN funcionamiento se visualizan los par metros activos Adem s la tecla SET permite efectuar cambios en el programa durante el funcionamiento despu s de pulsar la tecla PAUSE CONTINUE enter v Change up Change down Flecha ascendente y flecha descendente Cambian el valor par metro u otras variables en el campo intermitente Los valores se cambian de forma acelerada manteniendo oprimida una tecla y en incrementos prefijados con pulsaciones sucesivas El tipo de par metro o unidad por ejemplo Vh y las alternativas YES NO se cambian con una pulsaci n Las teclas tambi n pueden usarse para el cambio entre tiempo miliamperhoras y voltioshora en las modalidades RUN PAUSE y END Los valores se desplazan en pantalla cambiando autom ticamente entre m ximo y m nimo y viceversa RUN Pulsando la tecla RUN se inicia el proceso y se pone el programa en modalidad RUN La pantalla muestra el n mero de programa los valores actuales de tensi n amperaje y potencia as como el tiempo transcurrido voltioshora o miliamperhoras el cambio entre estos tres ltimos par metros se hace con las teclas Y f m gt z e Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD 9 4 Descripci n t cnica PAUSE CONTINUE Pone el aparato en modalidad de pause y desconecta la tensi n La pantalla muestra continue el estatus del proceso en el momento de pulsarse la tecla La
29. age and power During discontinuous electrophoresis however the voltage is not distributed evenly across the gel These gels have a region with low ionic strength that causes a high electrical field strength This region increases as the electrophoresis proceeds This means that the main part of the voltage is spread over a longer and longer distance and a higher and higher power is tolerated This is the reason why constant current is chosen for such applications It is however recommended to also limit the power as a precaution against overheating the gel The power supply will probably switch over to limiting power at the end of the run due to increased voltage The crossing over between different parameters controlling the electrophoresis can be illustrated by IEF isoelectric focusing using carrier ampholytes A graphical representation of the changes in power voltage and current that may occur during a typical IEF run is given in Fig 6 Since the pK values of the carrier ampholytes and the proteins are temperature dependent IEF must be carried out at a constant temperature Therefore cooling of electrophoresis equipment and controlling by power is recom mended The main part of the IEF is thus controlled by power phase II The conductivity is gradually decreasing because the carrier ampholytes and sample will loose their net charge during the build up of the pH gradient Thus the current will decrease and the voltage increase at constant
30. also possible to view the program settings Programming and running are discussed in more detail in the following two sections Blinking characters are shown as bold characters 5 2 Programming Start position When the power supply is switched on the display shows the start position in SET mode The previous program set is shown and that program number is blinking for example SET P1 OFF 3000 V 50mA 20 W If the power supply is switched on for the first time the breakpoint para meter and the alarm are off and the values for voltage current and power are 35 V 150 mA and 100 W respectively Choosing a program Up to 3 programs can be entered Press ENTER to confirm the program shown on the display or use Y keys to choose another Confirm with ENTER The programming procedure can be followed step by step in Fig 3 Setting voltage current and power limits The display will now flash for the set voltage limit Using the W keys select the maximum voltage desired for the run Confirm with ENTER Repeat the same procedure for limiting current and limiting power Programmable values for voltage are 35 3500 current 1 150 mA power 1 100 W time 0 01 500 h volthours 1 500 000 Vh Setting break point Choose between manual break or automatic break For automatic break choose breakpoint units in either Volthours Vh or time h Select the correct unit or for manual break choose OFF with yy Confirm with
31. amaci n avanzada y alta tensi n La EPS 3501 est disefiada principalmente para aplicaciones de Secuenciaci n de DNA Enfoque isoel ctrico IEF aunque tambi n es adecuada para A Blectroforesis en gel de poliacrilamida SDS PAGE PAGE nativa A Blectroforesis en gel de agarosa A Blectroforesis de DNA en campo pulsante Las separaciones electrofor ticas pueden controlarse por tensi n amperaje y potencia as como gradientes de tensi n para el control preciso de la electroforesis La EPS 3501 conmuta autom ticamente el par metro de control de acuerdo a los l mites programados y a las variaciones de conductividad del sistema m gt zZ e Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD 3 1 Introducci n E e E o un uy 4 Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD Seguridad 2 2 Seguridad 2 1 Medidas de seguridad La fuente de alimentaci n debe manejarse con suma precauci n puesto que puede desarrollar tensi n y amperaje suficiente para producir descargas mort feras VEN Para evitar riesgos de lesiones el aparato s lo debe manejarlo personal adecuadamente formado y siempre siguiendo las instrucciones adjuntas Leer este manual completo antes de utilizar la fuente de alimentaci n 1 El aparato est dise ado para uso exclusivo en interiores 2 El aparato debe utilizarse con el hilo de masa del
32. arameter values during the electrophoresis and final parameter values afterwards It also asks questions and shows error messages The display has an upper and lower row Fig 1 inside front cover shows the display in the start position when power is switched on for the first time The mode in this case SET is shown in the upper row on the left The program number is in the center of the upper row The number shown is that of the previously entered program As no program has yet been entered the first available program number P1 is shown The blinking figure in this case 1 indicates that it can be changed by using the Jy keys The upper right shows the programmed breakpoint In this case it is off The lower left middle and right positions show voltage current and power respectively 4 1 2 Keyboard ENTER Enters a value locks the value if correct and moves programming to the next field Valid values are voltage 35 3500 V current 1 150 mA power 1 100 W time 0 01 500 h volthours 1 500 000 Vh enter SET Puts the instrument into SET its programming and starting mode In RUN mode pressing SET also shows the programmed parameters for the actual run In addition SET allows you to make changes in the program during a run after first pressing PAUSE CONTINUE set V Change up Change down Changes the parameter value or other choice in the field which is blinking Numerical values are changed in an accelerating manner when
33. arately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Este producto cumple las disposiciones de la Directiva relativa a Bajo Voltaje 72 23 EEC a trav s de la norma armonizada NE 61010 1 1993 A1 1992 Normas EMC EMC Electromagnetic Compatibility Este producto cumple las disposiciones de la Directiva EMC 89 336 EEC a trav s de la normas armonizada NE 50081 1 emisiones y NE 50082 1 inmunidad Contenido Contenido 1 Introducci n 2 Seguridad 21 Medidas deseo cone 5 2 2 Funciones de seguridad incorporadas se 6 3 Desembalaje e instalaci n 4 Descripci n t cnica 4 1 Panel delantero ttt ttt ttt nenn 9 PN AD PONO AAA EE EEEE EEE EEE a RTE EEEE 9 41 2 Teclado roe ana 9 21 3 TOMAS DO saida sistere tte ettet taste teinte a aeii stes 10 4 2 lane 10 5 Operaci n 5 1 RESUMEN iii a 11 5 2 Instrucciones resumidas sscsssesessssssescsssssessssssesscsssseesssusesssssssecsssssessssssscesssseesssssssee 11 1ONVdS3 Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD 1 Contenido E e E o un uy 2 Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD Introducci n 1 1 Introducci n La fuente de alimentaci n EPS 3501 de GE Healthcare es un aparato seguro de gran calidad y precisi n para aplicaciones de electroforesis que requieren una progr
34. arr te automatiquement ou en appuyant sur la touche STOP Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD 11 FRANCE 5 Utilisation 12 Les caract res clignotants sont repr sent s en caract res gras Choisir un num ro de programme R gler la tension limite V R gler le courant limite mA Regler la puissance limite W Choisir l unit du param tre de fin h ou Vh ou pas OFF Desactive Fixer le parametre de fin Selectionner l option signal d alarme YES NO oui non Programme pr t SEE Pal 3000 V 50 mA s lectionner avec confirmer avec P2 OF 150 mA 100 W s lectionner avec confirmer avec SET Pe 2500 V 150 mA 100 W selectionner avec confirmer avec SEE P2 2500 V 50 mA s lectionner avec confirmer avec SETE 2500 V P2 50 mA s lectionner avec confirmer avec SEIE P2 2500 V 50mA s lectionner avec Param tre de fin confirmer avec SHPPUME AlarmatEnd Y s lectionner avec confirmer avec SEE pos 3000 Vh SET pa OFF 2500 V 50 mA 30W 2500 V 50 mA 30W Figure 3 R sum d taill de la programmation Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD Utilisation 5 1 Passer en mode MORE suite SEE p2A 3000 Vh 2500 V 50 mA 30W selectionner MORE avec 2 Selectionner le programme MORE P2 S lection programm
35. chlie en Den Strom einschalten Bei jedem Einschalten des Ger tes durchl uft dieses einen Selbstdiagnosetest Wird bei diesem Test ein Fehler entdeckt so erscheint eine Meldung auf der Anzeige und es ert nt ein Alarm Anschlu des der Elektrophoreseger tels Die von dem Elektrophoreseger t kommenden Kabel anschlie en Rot auf Rot Schwarz oder Blau auf Blau Abb 1 siehe Deckblattinnenseite Das rote Kabel ist das positive das blaue oder schwarze das negative Kabel ACHTUNG Stets intakte elektrische Kabel und Ausr stung verwenden die f r die zu verwendenden Spannungen geeignet sind Es k nnen zwei Elektrophoreseger te gleichzeitig mit demselben Programm betrieben werden Bitte beachten da die Grenzwerte f r Strom und Leistung verdoppelt werden m ssen wenn zwei Elektrophoreseger te gleichzeitig betrieben werden Die Spannung ist unabh ngig von der Anzahl der angeschlossenen Ger te gleich DEUTCH 3 Auspacken und Installation Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD Technische Beschreibung 4 4 Technische Beschreibung 41 Fronttafel Die Fronttafel besteht aus einer alphanumerischen Anzeige einer Tastatur mit 9 Folientasten einer Leuchtdiode LED die aufleuchtet wenn Spannung anliegt HV on sowie Anschl ssen f r zwei Elektrophoreseger te 4 1 1 Anzeige Eine 32 stellige alphanumerische Anzeige f hrt den Benutzer durch die Programm
36. e Sul pannello anteriore si trovano un display alfanumerico una tastiera con 9 tasti a membrana un diodo luminoso LED che si illumina quando viene applicata tensione HV attivata e connettori per due unit per elettroforesi 4 1 1 Display Un display alfanumerico a 32 cifre guida l operatore nella programmazione ed illustra i valori attuali dei parametri durante l elettroforesi nonch i parametri finali ad elettroforesi terminata Esso pone anche domande e visualizza i messaggi di errore Il display dotato di una riga inferiore ed una superiore La Fig 1 vedere prima di copertina mostra il display all attivazione dell unit Il modo in questo caso SET viene visualizzato nella riga superiore sulla sinistra Il numero del programma si trova al centro della riga superiore Il numero indicato quello del programma precedente La cifra lampeggiante in questo caso 1 indica che pu essere cambiato utilizzando i tasti W La riga superiore destra indica il punto di interruzione programmato In questo caso disattivato Le posizioni inferiore sinistra centrale e destra visualizzano rispettivamente tensione corrente e potenza 4 1 2 Tastiera ENTER t T sn Inserisce un valore fissa il valore se corretto e commuta la programmazione al campo successivo I valori disponibili sono tensione 35 3500 V corrente 1 150 mA potenza 1 100 W tempo 0 01 500 ore e voltore 1 500 000 Vh SET Commuta lo strumento in SET nel modo di
37. e s lectionner avec confirmer avec 3 S lectionner l option D marrage t du courant VES no s lectionner avec confirmation et sortie avec v rification du courant 4 Retour au mode SET SET p2A 3000 Vh programmation d marrage 2500 V 50 mA 30W Figure 4 D sactivation des v rifications du courant initial PRECAUTION Lorsqu on d sactive la fonction de v rification du courant initial l alimentation peut n anmoins fournir une haute tension m me si elle n est pas branch e sur l quipement d lectrophor se 1 Choisir le num ro de programme GET P1 OFF s lectionner avec 3000V 50mA 20W s lectionner avec 2 D marrer l ex cution du programme SEE P2 3000 Vh d marrer avec 2500V 50mA 30W d marrer avec F RUN p2A 1 Vh 500 6 mA 30 W RUN p2 500 Vh 1200V 25mA 25W ou attendre 5 sec 3 Pendant l ex cution du programme vous pouvez ou visualiser les BZSEIE p2A 3000 h param tres programm s Il est 2500 V 50mA 30W possible d interrompre l ex cution pour effectuer des modifications dans le programme SER P2 3000 Vh 2500V 50mA 30W PAUSE p2A 500 Vh 1200V 25mA 30W Programmation 4 Arr t manuel de l ex cution RUN p2A 2500V 10mA 2500 Vh 25 W END p2 2500 Vh 2500V 50mA 25W END P2 3000 Vh 2500V 9mA 20W 5 Arr t automatique de l ex cution Figure 5 R sum d tail
38. e Wipe regularly with a soft damp cloth Let the power supply dry completely before use If wetted unplug the power supply until the instrument is dry 6 Useonly undamaged electrical wire and equipment specified for the voltages you will use High voltages wires must meet the requirements of the IEC 1010 2 031 1995 electrical standard Any electrophoresis equipment connected to the power supply should meet the requirements of the IEC 1010 1 1993 electrical standard 7 Note that the output is connected to the chassis reference earth Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 9 ENGLISH 2 Safety information 10 2 2 Built in safety features The EPS 3501 has been tested and complies with the IEC 61010 EN 61010 1 electrical safety standard The power supply also has several built in safety functions Error messages are also shown on the display see 7 Trouble shooting 1 Functional earth leakage Should the power supply be connected to an electrophoresis unit that has a leakage path to earth the EPS 3501 will detect this fault and the high voltage is turned off 2 Start current check To ensure that an electrophoresis unit is connected correctly the power supply checks that the resistance is not higher than a specified limit at a low safety voltage 40V If this resistance is too high the voltage is turned off Too high a resistance can also be caused by using buffers with extremely low
39. e Bio Sciences AB and contractual approval if required to make changes in specifications and features shown Bj rkgata n 30 nn or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information 751 84 Uppsala Sweden 2006 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric Company GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 852 2811 8693 Fax 852 2811 5251 Australasia Tel 61 2 9899 0999 Fax 61 2 9899 7511 Austria Tel 01 57606 1619 Fax 01 57606 1627 Belgium Tel 0800 73 888 Fax 03 272 1637 Canada Tel 800 463 5800 Fax 800 567 1008 Central East amp South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 Denmark Tel 45 16 2400 Fax 45 16 2424 e Finland Baltics Tel 358 0 9 512 39 40 Fax 358 0 9 512 39 439 e France Tel 01 69 35 67 00 Fax 01 69 41 96 77 e Germany Tel 0761 4903 490 Fax 0761 4903 405 Italy Tel 02 27322 1 Fax 02 27302 212 Japan Tel
40. e s rieusement endommag si l unit Employer le cordon d alimentation secteur appropri et brancher une de ses extr mit s sur le r ceptacle d alimentation secteur de l unit EPS 3500 voir Figure 2 et l autre extr mit la prise d alimentation secteur avec prise de terre Mettre l unit sous tension Chaque fois que l instrument est mis sous tension un essai automatique est effectu par le syst me Si cet essai d tecte une erreur il affiche un message l cran d affichage et d clenche un signal d alarme sonore voir 2 2 Branchement de ou des unit s d lectrophor se Brancher les fils de l unit d lectrophor se rouge sur rouge et noir ou bleu sur bleu comme indiqu dans Figure 1 voir verso de la page de couverture Le fil rouge est le positif et le fil noir ou bleu est le n gatif RECOMMANDATION IMPORTANTE N utiliser que des fils lectriques parfaitement intacts et des quipements autoris s pour la tension utilis e Il est possible d utiliser deux unit s d lectrophor se simultan ment avec le m me programme Il est indispensable de doubler les limites d intensit mA et de puissance W lorsqu on utilise deux unit s d lectrophor se en m me temps Par contre la tension V reste la m me qu on utilise une ou deux unit s d lectrophorese la fois gt gt Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD 7 FRANCE 3 Reception et
41. empo impostare il valore da utilizzare come punto di interruzione con J e ENTER 6 Nel caso in cui sia stato selezionato un punto di interruzione inferiore a 4 il display chieder all operatore se desidera l emissione un allarme acustico altermine del funzionamento Selezionare YES o NO con Y e ENTER premere La selezione di YES viene indicata sul display da una piccola A sulla destra del numero del programma cio 1A 7 Una volta completata la programmazione collegare l unit per elettroforesi alle prese e premere RUN 8 Suldisplay vengono visualizzati gli attuali valori di tensione corrente e potenza insieme a tempo trascorso o voltore commutare con YW N 9 PAUSE CONTINUE pu essere utilizzato per interrompere e riprendere il funzionamento 10 Durante il funzionamento i parametri programmati possono essere controllati premendo SET 11 Il funzionamento si interrompe automaticamente o premendo STOP gt gt Z Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD 11 5 Funzionamento caratteri lampeggianti vengono illustrati in neretto 1 Selezionare il numero SET P1 del programma 3000V 40mA Selezionare con Confermare con 2 Selezionare il limite di a P2 ORF tensione 150 mA 100 W Selezionare con Confermare con 3 Selezi il limite di elezionare il limite di SER ES OFF corrente 2000 V 150 mA 100 W Selezionare con 0 9 Co
42. er R ckwand befinden sich 1 Ein Netzschalter Durch Dr cken von wird der Strom zum Stromversorgungsger t eingeschaltet Durch Dr cken von O wird der Strom abgeschaltet 2 Eine Buchse f r das Netzkabel 3 Ein Schalter f r den Spannungsbereich Die linke Schalterstellung entspricht dem Bereich 100 120 V die rechte dem Bereich 220 240 V 4 Bel ftungs ffnungen Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD Betrieb 5 5 Betrieb 5 1 berblick Der Betrieb des EPS 3501 wird in der nachfolgenden Ablauffolge beschrieben 1 Programmieren oder Bearbeiten eines Verfahrens siehe Abb 3 und Abb 4 2 Abarbeiten eines Verfahrens siehe Abb 5 5 2 Kurzanweisungen Dieses Kapitel fa t die Hauptpunkte f r den Betrieb des EPS 3501 zusammen Es wird auch auf die separate schematische Betriebsanleitung verwiesen die dem Stromversorgungsger t beiliegt Es wird empfohlen diese schematische Anleitung stets in der N he des Ger ts aufzubewahren 1 Den Netzstrom einschalten Auf der Anzeige sollte die Programmnummer blinken 2 ENTER f r die aktuelle Programmnummer dr cken oder mit den Tasten J die gew nschte Programmnummer w hlen ENTER dr cken 3 F r jeden der Parameter Spannung Strom und Leistung so lange J dr cken bis der gew nschte Wert erreicht ist Durch Dr cken von ENTER jeden Parameter best tigen wor auf die Anzeige automatisch auf den n chsten Paramete
43. es anna 11 Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD 1 FRANCE Table des mati res Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD Introduction 1 1 Introduction L unit d alimentation EPS 3501 pour lectrophor se produite par GE Healthcare est une unit d alimentation lectrique de grande qualit de haute pr cision et de s curit maximale pour les applications d lectrophor se qui n cessitent Une tension maximum de 3500 Volts L EPS 3501 est destin e en premier lieu aux techniques suivantes s quen age d ADN focalisation iso lectrique IEF Toutefois l EPS 35501 peut aussi tre utilis e pour lectrophor se en gel de polyacrylamide SDS PAGE PAGE native lectrophor se Agarose lectrotransfert lectrophor se d ADN champ en puls Les s parations lectropho tiques peuvent tre contr l es par la tension le courant ou la puissance L EPS 3501 bascule automatiquement sur le param tre de contr le en fonction des limites programm es et en fonction des variations de conductivit dans le syst me Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD 3 FRANCE Introduction Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD Renseignements concernant la s curit 2 2 Renseignements concernant la s curit 2 1 Pr cautio
44. esis Power Supply EPS 3501 18 1130 04 Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 29 ENGLISH 9 Ordering information 30 Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Important user information Normes de securit Veuillez ce manuel enti rement pour une compr hension totale de l usage de ESP 3501 AVERTISSEMENT Le signe d avertissement souligne une instruction qui doit tre scrupuleusement suivie afin d viter des blessures corporelles Ne pas poursuivre tant que les instructions n ont pas t enti rement assimil es et que toutes les conditions indiqu es n ont pas t r unies CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request Le symbole CE et la d claration de conformit correspondante s appliquent l instrument lorsqu il est utilis comme unit seule ou connect d autres instruments GE Healthcare marqu s CE ou connect d autres produits recommand s ou d crits dans ce
45. etic Compatibility Il prodotto conforme alla direttiva sulla compatibilt elettromagnetica 89 336 EEC in base alle norme armonizzata EN 50081 1 emission EN 50082 1 immunit Indice Indice 1 Introduzione 2 Informazioni sulla sicurezza 2 1 Norme di SICUrezzd 2 2 Funzioni di sicurezza incorporate 3 Disimballaggio ed installazione 4 Descrizione tecnica 4 1 Pannello ANtErIOre ii nanki 9 4 1 1 Display 4 1 2 Tastiera ALS Pfese di USCITO ne ise Ree eU ede bre ila 10 4 2 Pannello posto cia 10 5 Funzionamento Lubiana 11 E AA ene AA anni 11 gt c 2 Z Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD 1 Indice fe Z lt lt 2 Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD Introduzione 1 1 Introduzione L alimentatore per elettroforesi GE Healthcare EPS 3501 un unit di sicurezza ad alta qualit e precisione per applicazioni di elettroforesi che richiedono un massimo di 3500 volt L EPS 3501 stato concepito principalmente per le seguenti tecniche e Riordinamento di sequenza del DNA e EF focalizzazione isoelettrica L EPS 3501 anche adatto per e SDS PAGE Elettroforesi con gel poliacrilammide e PAGE nativa e Elettroforesi di agarosio e Elettroassorbimento e Elettroforesi pulsata del DNA Le separazioni per elettroforesi possono essere cont
46. gaben N 2 1 SicherheitsmaBnahmen Da das Ger t gen gend Spannung und Strom entwickeln kann um einen t dlichen elektrischen Schlag zu verursachen wird bei dessen Betrieb um u erste Vorsicht gebeten Zur Vermeidung von Verletzungsgefahren sollte das Ger t nur von entsprechend geschultem Personal und stets gem den beiliegenden Anweisungen betrieben werden Das Handbuch vor der Benutzung dieses Stromversorgungsger tes vollst ndig durchlesen 1 Das Ger t ist nur f r den Gebrauch in geschlossenen R umen geeignet 2 Der Schutzerdleiter des Stromversorgungskabels mu beim Gebrauch des Ger tes stets korrekt am Netzausgang geerdet sein 3 Um eine ausreichende K hlung zu erm glichen ist zu gew hrleisten da die Bel ftungs ffnungen hinten und an den Seiten des Ger tes nicht verdeckt sind 4 Das Ger t nicht bei hoher Luftfeuchtigkeit ber 95 betreiben Kondensation vermeiden indem man das Ger t nach einem Transport von einer k lteren in eine w rmere Umgebung sich stets auf Umgebungstemperatur erw rmen l t 5 Das Ger t so trocken und sauber wie m glich halten Regelm ig mit einem weichen und feuchten Tuch abwischen Vor dem Gebrauch das Stromversorgungsger t vollst ndig trocknen lassen Vor dem Befeuchten stets den Netzstecker des Ger tes ziehen und ihn erst dann wieder einstecken wenn es trocken ist 6 Stets intakte elektrische Kabel und Ausr stung verwenden die f r die zu verwe
47. ht Maximum voltage current and power with automatic cross over at preset limits Voltage 0 3500 V DC Current 0 150 mA Power 0 100 W Voltage 35 3500 V DC Current 1 150 mA Power 1 100 W Time 00 01 500 h Volthour 1 500 000 Vh Voltage 1V Current 100 pA 0 14 9 MA 1 mA 15 150 mA Power 1W Voltage 5V Current 1 mA Power 1W Time 1 min 00 01 99 59 1h 100 500 h Volthour 1 Vh 1 9999 Vh 100 Vh 10 0 99 9 kVh 1kVh 100 500 kVh Voltage 1 5V Current 1 1mA Power 2 1W Timer 0 1 1 min 00 01 99 59 h 0 1 10 min 100 500 h lt 0 2 lt 1 at load change 10 90 of maximum load lt 1 at 3500 V lt 0 2 10 h after warm up lt 1 year Resistance not greater than 1 M at 40 V current less than 40 pA Can be disabled Shuts off output voltage on extreme load change Leakage not greater than 500 pA Fully protected against any overload conditions Duration lt 8 s The program continues automatically Duration 8 s The program continues after manual restart 4 40 C 0 95 84 106 kPa according to GPR 100 120 220 240 V 50 60 Hz Max 140 W 250 x 315 x 95 mm 3 8 kg Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 27 HSI19N3 ENGLISH 8 Technical specifications 28 Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Ordering information 9 HSI19N3 9 Ordering information Designation Code No Electrophor
48. ic test is done If an error is detected during the test a message will appear on the display and an alarm will sound gt gt gt Connection of the electrophoresis unit s Connect the leads from the electrophoresis unit red to red and black or blue to blue see Fig 1 inside front cover The red lead is the positive and black or blue is the negative WARNING Use only undamaged electrical wire and equipment approved for the voltage you will use Two electrophoresis units can be run simultaneously with the same program Please remember to double the limiting current and power if two electrophoresis units are run at the same time The voltage will be the same regardless of the number of units Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 11 ENGLISH 3 Unpacking and installation 12 Local regulation for Great Britain WARNING IMPORTANT This appliance must be earthed The wires in the mains lead are coloured in accordance with the following code Green and yellow Earth Blue Neutral Brown Live If the plug provided is unsuitable for socket outlets the plug must be cut off and suitable plug fitted The cut off plug should be disposed of and must not be inserted into any 13 amp socket as this can result in electric shock The plug or adapter or the distribution panel should be provided with 13 amps fuse As the colours of the wires in the mains lead of this appliance may not correspond with c
49. ierung zeigt aktuelle Parameterwerte w hrend der Elektrophorese sowie Endparameterwerte danach Sie stellt auch Fragen und zeigt Fehlermeldungen an Die Anzeige hat eine obere und eine untere Reihe Abb 1 siehe Deckblattinnenseite zeigt die Anzeige in der Startposition wenn der Strom eingeschaltet ist Der Modus in diesem Fall SET wird in der oberen Reihe auf der linken Seite angezeigt Die Programmnummer befindet sich in der Mitte der oberen Reihe Die angezeigte Nummer ist die Nummer des Programms in das der Benutzer zuvor gegangen ist Die blinkende Zahl in diesem Fall 1 zeigt an da sie durch Dr cken der Tasten y ge ndert werden kann Die obere Reihe auf der rechten Seite zeigt den programmierten Stopparameter Diese Funktion ist in diesem Falle abgeschaltet Die Positionen unten links in der Mitte und rechts zeigen jeweils die Spannung den Strom und die Leistung an 4 1 2 Tastatur ENTER Durch Dr cken dieser Taste wird ein Wert eingegeben der wenn er korrekt ist aufgenommen wird und der Programmiervorgang geht auf das n chste Feld ber G ltige Werte sind f r die Spannung 35 3500 V f r den Strom 1 150 mA f r die Leistung 1 100 W f r die Zeit 0 01 500 h f r die Voltstunden 1 500 000 Vh SET Diese Taste schaltet das Ger t in den SET Modus seinen Programmierungs und Startmodus Im RUNModus werden nach dem Dr cken der Taste SET die programmierten Parameter f r den aktuellen Programmlauf angezeigt
50. iesem Fall wird die Hochspannung abgeschaltet und eine Fehlermeldung angezeigt Diese Funktion kann f r die Durchf hrung bestimmter Anwendungen gesperrt werden siehe Abb 4 Sperren der Strompr fung 3 Sudden load change detection Lastwechsel Pr fung Mit dieser Funktion werden Unf lle unter Programmlaufbedingungen aufgrund eines Zusammenbruchs der elektrischen Schaltung wie zum Beispiel aufgrund einer schlechten Verbindung mit dem Elektrophoreseger t verhindert In einem solchen Fall wird die Hochspannung abgeschaltet Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD Auspacken und Installation 3 3 Auspacken und Installation gt gt Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD Auspacken Den Inhalt anhand der beigef gten Packliste pr fen Sollte das Ger t beim Transport besch digt worden sein sofort die rtliche GE Healthcare Niederlassung und das entsprechende Speditionsunternehmen verst ndigen Netzanschlu Den entsprechenden Spannungsbereich w hlen 100 120 oder 220 240 V Abb 2 siehe Deckblattinnenseite ACHTUNG Wird der Spannungsbereich auf 100 120 V eingestellt und das Ger t an 220 240 V angeschlossen dann wird das Ger t stark besch digt Das entsprechende Netzkabel w hlen und ein Ende an die Netzanschlu buchse des EPS 3501 Stromversorgungsger tes seihe Abb 2 und das andere Ende an eine geerdete Netzsteckdose ans
51. ing You can interrupt the electrophoresis for sample loading and or changing parameter values by pressing PAUSE CONTINUE Voltage and current will no longer be supplied the HV on LED goes off and you may safely load your samples The display shows the status of the run when PAUSE CONTINUE was pressed Switch between time and integrated voltage by pressing yy When sample loading is complete press PAUSE CONTINUE again or RUN and the run will continue from where it was interrupted When in the PAUSE mode you can also press SET to make changes in the program This mode is called the P SET mode When the P SET mode is entered you can make changes as described under programming The P SET mode is the same as the SET mode apart from restrictions in setting the breakpoint Naturally it is not possible to enter a time or volthour that is already passed Press EXIT or PAUSE CONTINUE to go back from P SET to PAUSE Press RUN or PAUSE CONTINUE to proceed with the electrophoresis Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 17 ENGLISH 5 Operation 18 View programmed values It is also possible to view the programmed values during a run by pressing SET Note that no values can be changed here The display returns automatically to show RUN values after 5s Alternatively use EXIT or RUN Stopping the run and viewing end parameter values When the programmed time or volthour is attained the program will enter the End m
52. io autom tico fijar el valor a utilizar para el punto de cambio yy con las teclas y pulsar ENTER 6 Si se ha elegido el evento seg n el punto 4 el sistema pregunta si se desea que suene la alarma al terminar el proceso Seleccionar s YES o no NO con las teclas J T y pulsar ENTER Si se elige alarma ello se indica con una A peque a en supraindice como en 1A a la derecha del n mero de programa en la pantalla 7 Al terminar la programaci n conectar la unidad electrofor tica a la toma y pulsar la tecla RUN m D gt z e 8 La pantalla muestra los valores actuales de tensi n amperaje y potencia as como el tiempo transcurrido o los voltioshora conmutar las teclas yy 9 Latecla PAUSE CONTINUE puede usarse para interrumpir y continuar el funcionamiento 10 Los par metros programados pueden controlarse durante el funcionamiento pulsando la tecla SET 11 El proceso programado se para autom ticamente pulsando la tecla STOP Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD 11 ESPANOL 5 Operaci n Los caracteres intermitentes se visualizan en negrita 1 Elegir el numero de SET P1 programa 3000 V 40 mA Seleccionar con Confirmar con 2 Fijar el limite de tensi n Pe OFF 150 mA 100 W Seleccionar con Confirmar con 3 Fijar el l mite de amperaje SET P2 OFF 2000 V 1500 mA 100W Seleccionar con Confirmar con 4 Fijar el l
53. is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual AVVERTIMENTO Questo e un prodotto del codice Categoria A In un ambiente domestico questo prodotto pu causare l interferenza radiofonica nel qual caso l utente pu essere tenuto ad approntare le misure sufficienti Recycling This symbol indicates that the waste of electrical R and electronic equipment must not be disposed as unsorted municipal waste and must be EE collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Questo prodotto conforme alla Direttiva sulla bassa tensione LVD 72 23 EEC in base alla norma armonizzata EN 61010 1 1993 A1 1992 Standard EMC EMC Electromagn
54. l des fonctions RUN et PAUSE Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD 13 FRANCE 5 Utilisation 14 Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD Important user information Sicherheitsnormen Zum vollen Verst ndnis vor Gebrauch von EPS 3501 dieses Handbuch sorgf ltig lesen WARNUNG hin die unbedingt eingehalten werden mu um Verletzungen zu varhindren Sorgen Sie daf r da Sie erst dann fortsetzen wenn Sie die Anleitungen eindeutig verstaden haben und wenn alle erforderlichen Scherheitsma nahmen getroffen wurden 1 Das Zeichen WARNUNG deutet auf eine Anweisung CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request Das CE Symbol und die sich dadurch ergebende Erkl rung bez glich Einhaltung der Vorschriften ist nur dann f r das Intsrument g ltig wenn es auf folgende Weise verwendet wird Es wird als alleinstehendes Ger t verwendet Es wird an andere Instrumente der Firma
55. leitung 18 1130 21 Edition AD 11 DEUTCH 5 Betrieb 12 Blinkende Zeichen sind im Fettdruck dargestellt Programmnummer w hlen Spannungsgrenzwert einstellen Stromgrenzwert einstellen Leistungsgrenzwert einstellen W hlen des Zwischenstoppara meters h oder Vh oder kein Zwischenstop OFF AUS Zwischenstoppara meter einstellen Alarm YES oder NO w hlen Programm bereit SEIE P1 5000 V 40 mA 0 Wahlen mit 0 Best tigen mit Pz 150 mA W hlen mit Best tigen mit SEE pa OFF 2000 V 150 mA 100 W W hlen mit Best tigen mit SEIE P2 2500V 50 MA W hlen mit Best tigen mit SET 2500 V PE 50 MA W hlen mit Best tigen mit SEIE PE 2500V 50mA W hlen mit Best tigen mit W hlen mit Best tigen mit SEE 2500 V P2 50 MA SEIE poa 3000 Vh 2500 V 50 mA 30W Abb 3 Schrittweise Zusammenfassung des Programmiervorgangs Zwischenstopparamet er aus Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD Betrieb 5 1 MORE eingeben SET P2A 3000 Vh 2500 V 50 mA 30W MORE w hlen mit 2 Programm w hlen MORE P2 Select program W hlen mit Best tigen mit Current check at start YES no W hlen mit M g E 3 Strompr fung ja nein w hlen Best tigen und Verlassen mit 4 R ckkehr zum SET Modus SET p2A 3000 Vh 2500 V 50 mA 30 W Abb
56. n at any stage during programming in SET mode by pressing EXIT or STOP Note that the program is saved automatically when EXIT is pressed Disabling the current check See Section 5 4 if you want to use this feature 5 3 Running a program Connect the leads from the electrophoresis unit red to red and black or blue to blue Red is positive and black or blue negative Up to two electrophoresis units can run at the same voltage at one time Please remember to double the maximum current and power conditions if two units are to be run Voltage will be the same regardless of how many units are run The current should also be doubled if two gels are run on the same electrophoresis unit Choosing a program Press SET and select the program you wish to run by pressing J 4 until the value after the P is correct Confirm with ENTER Omit this step if you have just programmed a method as described insection 5 2 Running a program is described schematically in Fig 4 Running Press RUN to start the electrophoresis The display will show current values for voltage current power and elapsed time or volthours You can switch between showing the elapsed time or volthours by J f The limiting parameter will attain the programmed limit The unit for that parameter is underlined A light emitting diode shows when voltage is applied HV on If no current is displayed please check the electrical connections to the electrophoresis equipment Paus
57. ndenden Spannungen geeignet sind Hochspannungskabel sollten der elektrischen Sicherheitsnorm IEC 1010 2 031 1993 entsprechen S mtliche an die Stromversorgung angeschlossene Elektrophorese Ausr stung sollte der elektrischen Sicherheitsnorm IEC 1010 1 1993 entsprechen 7 Man beachte da der Ausgang an Gehduse Referenzerde angeschlossen ist Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD 5 DEUTCH 2 Sicherheitsangaben 2 2 Eingebaute SicherheitsmaBnahmen Das EPS 3501 wurde gem der elektrischen Sicherheitsnorm IEC 61010 1 EN 61010 1 gepr ft Das Stromversorgungsger t verf gt auch ber mehrere eingebaute Sicherheitsfunktionen Die folgenden Fehlermeldungen werden auf der Anzeige angezeigt 1 Functional earth leakage Fehlerstromanzeige Wird das Stromversorgungsger t an ein Elektrophoreseger t angeschlossen das einen Erdschlu aufweist dann erfa t das EPS 3501 diesen Fehler und die Hochspannung wird abgeschaltet 2 Start current check Strompr fung starten Um zu gew hrleisten da ein Elektrophoreseger t richtig angeschlossen ist berpr ft das Stromversorgungsger t ob der Widerstand h her ist als ein vorgegebener Grenzwert bei einer niedrigen Sicherheitsspannung 40 V Ist dieser Widerstand zu hoch dann wird die Spannung abgeschaltet Ein zu hoher Widerstand kann auch durch die Verwendung von Puffern mit duBerst niedriger Leitf higkeit verursacht werden Auch in d
58. nfermare con 4 Selezionare il limite di SET p2 potenza 2500 V 50 mA Selezionare con Confermare con 5 Attivare il punto di interruzione h o Vh o disattivare il punto di interruzione OFF SETE 2500 V P2 50 mA Selezionare con Confermare con 6 Selezionare il punto di interruzione P2 50mA SEE 2500 V Selezionare con Punto di interruzione Confermare con disattivato 7 Attivare o disattivare gt allarme VES o NO Allarme finale Y Selezionare con A 9 Confermare con 8 Programma pronto SET p2 3000 Vh SET p2 OFF 2500 V 50 MA 30 W 2500 V 50 mA 30 W Fig 3 Riepilogo passo dopo passo di programmazione o PA lt lt 12 Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD Funzionamento 5 1 Inserire MORE SEE p2 3000 Vh 2500 V 50 mA 30W Selezionare MORE con 2 Selezionare il programma MORE P2 Selezionare programma W 3 Attivare o disattivare il controllo Ganuralla Gala connie cl della corrente avviamento YES no 8 W Selezionare con Confermare con Selezionare con Confermare e uscire con 4 Tornare al modo SET SET P2 3000 Vh 2500 V 50 mA 30 W Fig 4 Disabilitazione del controllo della corrente AVVERTENZA Disabilitando il controllo della corrente di avviamento l alimentatore pu fornire alta tensione anche se non collegato all attrezzatura per elettroforesi gt
59. ng il lea 9 AL a ET 9 41 2 TOSCO isc 9 4 1 3 Ausgangsbuchsen aaa ana a aaeei 10 4 2 Md aua 10 5 Betrieb 5 1 Ibi 11 ls AA A eres eeeecestns 11 Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD 1 DEUTCH Inhaltsverzeichnis Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD 1 Einleitung Einleitung 1 Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD Das Elektrophorese Stromversorgungsger t EPS 3501 von GE Healthcare ist ein qualitativ hochwertiges u erst genaues und sicheres Stromversorgungsger t f r Elektrophoresea nwendungen die eine Stromversorgung von maximal 3500 Volt ben tigen Das Modell EPS 3501 wurde vornehmlich f r die folgenden Arbeitsverfahren entwickelt DNA Sequenzierung e IEF Isoelektrische Fokussierung Das Modell EPS 3501 ist auch geeignet f r e SDS PAGE Polyacrylamidgel Elektrophorese e Native PAGE e Agarose Elektrophorese e Elektroblotting DNA Pulsfeld Elektrophorese Elektrophoresetrennungen k nnen mit Spannung Strom oder Leistung gesteuert werden Die Steuerparameter V mA W werden in Abh ngigkeit von den einprogrammierten Grenzwerten sowie von der Leitf higkeit w hrend des Laufes automatisch vom EPS 3501 angepa t DEUTCH 1 Einleitung Electrophoresis Power Supply EPS 3501 Bedienungsanleitung 18 1130 21 Edition AD Sicherheitsangaben 2 2 Sicherheitsan
60. nit or for manual break choose OFF with WR Confirm with ENTER 5 Ifvolthours or time were chosen set the value to be used for the breakpoint with and ENTER 6 If an automatic breakpoint was chosen under 4 you will be asked if you wish an alarm to sound at the end of the run Select YES or NO with 4 and press ENTER If YES was chosen this is indicated by a small A as in 1A to the right of the program number in the display Preset time Alarm Fig 6 Power fluctuations which occur during a typical IEF run 7 When programming is completed connect your electrophoresis unit to the outputs and press RUN 8 The actual voltage current and power are shown on the display together with the elapsed time or volthours switch with 4 4 9 PAUSE CONTINUE can be used to interrupt and then continue the run 10 The programmed parameters can be checked during the run by pressing SET 11 The programmed run stops automatically or by pressing STOP Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Maintenance 6 HSI19N3 6 Maintenance Wipe the instrument regularly with a damp cloth Let the instrument dry completely before use Otherwise no user maintenance is necessaru All servicing should be entrusted to qualified personnel only Please contact your local GE Healthcare representative for more service information Recycling This symbol indicates that the waste of electrical and elect
61. ns Op rer avec la plus grande prudence lorsque vous utilisez l instrument car il peut g n rer une tension et une intensit suffisantes pour provoquer des chocs lectriques mortels N Pour viter tout risque d accident l instrument doit tre utilis uniquement par du personnel qualifi et toujours en suivant strictement les instructions fournies Nous vous recommandons de lire attentivement ce manuel avant d utiliser l unit d alimentation lectrique 1 Cetinstrument a t concu pour tre utilis l int rieur uniquement 2 Cetinstrument doit toujours tre utilis avec le fil de terre du cordon d alimentation correctement mis la terre de la prise secteur 3 Pour que la ventilation fonctionne normalement veiller ce que les fentes d a ration l arri re et sur les c t s de l instrument ne soient jamais obstru es 4 Ne pas faire fonctionner l instrument en condition d humidit extr me audessus de 95 viter la condensation en laissant l unit s quilibrer la temp rature ambiante lorsqu on la fait passer d un environnement froid un environnement plus chaud 5 Maintenir l instrument aussi sec et propre que possible Essuyer l instrument intervalles r guliers avec un chiffon doux humide Laisser s cher l unit d alimentation compl tement avant toute utilisation Lorsque l instrument est humide d brancher l alimentation jusqu ce que l instrument soit sec 6 N utiliser que des
62. o de tensi n la posici n izquierda corresponde a 100 120 V y la derecha a 220 240 V 4 Respiraderos e lt a 4 Lu 10 Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD Operaci n 5 5 Operaci n 5 1 Resumen La operaci n de la fuente de alimentaci n EPS 3501 queda descrita brevemente en esta secuencia 1 Programaci n o edici n de un m todo Ver las figuras 3 y 4 2 Ejecuci n de un m todo Ver la figura 5 5 2 Instrucciones resumidas En este cap tulo se resumen los puntos m s importantes para el manejo correcto de la EPS 3501 Ver tambi n la gu a de manejo esquem tica entregada con el equipo Se recomienda mantener la gu a junto al mismo 1 Conectar a la red La pantalla debe mostrar el n mero de programa intermitentemente 2 Pulsar la tecla ENTER del n mero de programa actual o seleccionar el n mero de programa deseado con las teclas Y T Pulsar ENTER 3 Para cada uno de los par metros de tensi n amperaje y potencia pulsar y hasta alcanzar el valor previsto Confirmar con la tecla ENTER despu s de cada selecci n la pantalla vuelve autom ticamente al par metro siguiente 4 Elegir entre cambio manual o autom tico Para el cambio autom tico seleccionar las unidades del evento Voltioshora Vh o tiempo h Utilizar las teclas 4 4 para la selecci n de unidades OFF para cambio manual Confirmar con la tecla ENTER 5 Enel modo camb
63. ode It is also possible to break the run manually by pressing STOP In both cases the voltage current and power will go to zero as indicated by the HV on LED switching off The end parameter values are displayed Switch between elapsed time and integrated voltage by y An alarm will sound if selected in the program You can stop the alarm by pressing STOP Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Operation 5 1 Choose Program number SET P1 OFF 3000V 50mA 20W HSI19N3 Select by 2 Start the run SET P2 3000 Vh 2500V 50mA 30W Start by E RUN p2 1 Vh 500 6 mA 30 W Y a RUN pee 500 Vh 1200V 25mA 25W C lorwaitss 3 During a run you can view the settings It is also possible to interrupt the run and make changes DESEE p2 3000 h in the program 2500V 50mA 30W SEIE P2 3000 Vh 2500V 50mA 30W PAUSE p2 500 Vh 1200V 25mA 30W Programming 4 Stop the run manually RUN poA 2500 Vh 2500V 10mA 25W END p2 2500 Vh 2500V 50mA 25W 5 The run is stopped automatically PP J END P2 3000 Vh 2500V 9mA 20W Fig 4 Step by step summary of run and pause function A run cannot be continued after pressing STOP Disconnect the leads and proceed with post electrophoretic techniques Since diffusion will begin as soon as the voltage is turned off you should remove the gel and begin s
64. oloured markings identifying the terminals in your plug proceed as follows The green and yellow wire must be connected to the terminal in the plug which is marked with the letter E or by the earth symbol or coloured green or green and yellow The blue wire must be connected to the terminal which is marked with the letter N or coloured black The brown wire must be connected to the terminal which is marked with the letter L or coloured red Note After replacing or changing a fuse the fuse cover in the plug must be replaced with a fuse cover which corresponds to the colour of the insert in the base of the plug or the word that is embossed on the base of the plug and the appliance must not be used without a fuse cover Only 13 Amps fuse approved to B S 1362 A S T A should be used Switch on the power Each time the instrument is turned on a self diagnostic test is done If an error is detected during the test a message will appear on the display and an alarm will sound Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Technical description 4 HSI19N3 4 Technical description 4 1 Front panel The front panel consists of an alphanumeric display a keyboard with 9 membrane keys a light emitting diode LED that lights when voltage is applied HV on and connectors for two electrophoresis units 4 1 1 Display A 32 digit alphanumeric display guides you through the programming shows current p
65. ontents HSI19N3 Contents 1 Introduction 2 Safety information 2l rai 2 2 Built in safety features 3 Unpacking and installation 4 Technical description Ul Fe era 13 UL DS 13 4 1 2 KeuboGEd csi itenim etim dnte ea te tein 13 ATS Output SOCKeLS eerie d dpan AA uc iet 14 RE eee ne tee Onn re ee nee mee 14 5 Operation 5 1 OVOP VIOW E E OU etne A O 53 RUNNING PU Ra 5 4 Optional programming 5 5 Choosing run parameters e UMN MISTS seieren eE EEEE EEOAE ASETET MAMTENAMCE A 23 Trouble shooting M 25 Technical specifications ces 27 0 N A Ordering Niola 29 Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 5 ENGLISH Contents Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Introduction 1 HSITDN3 1 Introduction The GE Healthcare Electrophoresis Power Supply EPS 3501 is a high quality high precision and safe power supply for electrophoresis applications that require a maximum of 3500 volts EPS 3501 is primarily designed for the following techniques e DNA sequencing e IEF Isoelecric focusing EPS 3501 is also suitable for e SDS PAGE Polyacrylamide Gel Electrophoresis e Native PAGE Agarose electrophoresis Electroblotting e DNA pulsed field electrophoresis Electrophoresis separations can be controlled by voltage current or power The EPS 3
66. ors tension 2 Un r ceptacle pour le c ble d alimentation 3 Un commutateur de tension secteur de l instrument La position gauche correspond 100 120 V et la position droite 220 240 V 4 Des ouvertures d a ration Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD Utilisation 5 5 Utilisation 5 1 G n ralit s Le fonctionnement de l EPS 3501 est d crit dans l ordre suivant 1 Programmation ou s lection d une proc dure voir Figures 3 et 4 2 Ex cution d une proc dure voir Figure 5 5 2 Instructions simplifi es Cette section est un r sum des principaux points retenir pour l utilisation de l EPS 3501 Nous vous recommandons aussi de vous r f rer au sch ma du guide d utilisation fourni avec l unit d alimentation lectrique Conserver ce guide d utilisation pr s de l instrument 1 Mettre l instrument sous tension ON Le num ro de programme doit clignoter sur l cran d affichage 2 Appuyer sur la touche ENTER pour valider le num ro de programme m moris ou utiliser les touches Y A pour s lectionner le num ro de programme souhait Appuyer sur la touche ENTER 3 Pour chacun des param tres Tension Courant et Puissance appuyer sur une des touches Y jusqu ce que la valeur s lectionn s affiche Confirmer la s lection en appuyant sur la touche ENTER apr s chacune de ces valeurs et l cran affiche automatiquement le param tre suivan
67. per elettroforesi sia collegata correttamente l alimentatore controlla che la resistenza non superi un limite specifico a bassa tensione 40 V Se la resistenza troppo elevata viene disattivata l alimentazione Una resistenza troppo elevata pu essere dovuta anche all utilizzo di tamponi con una conduttivit estremamente ridotta L alimentazione viene disattivata anche in questo caso ed appare un messaggio di errore Questa funzione pu essere disabilitata per alcune applicazioni vedere Fig 4 disattivazione del controllo della corrente 3 Rilevamento di cambi di carico improvvisi Questa funzione previene incidenti durante il funzionamento dovuti ad un guasto nell impianto elettrico come ad esempio un collegamento errato all unit per elettroforesi In tal caso viene disattivata l alta tensione fe PA lt lt 6 Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD Disimballaggio ed installazione 3 3 Disimballaggio ed installazione gt gt Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD 7 Disimballaggio Accertarsi che il contenuto della confezione sia conforme all elenco allegato Controllare che lo strumento non abbia subito danni durante il trasporto Qualora vi siano danni rivolgersi immediatamente al rappresentante GE Healthcare ed alla ditta di trasporti interessata Collegamento alla rete principale Selezionare la tensione appro
68. priata 100 120 o 220 240 V Fig 2 vedere prima di copertina AVVERTENZA Se l alimentatore viene collegato ad una tensione di 220 240 V mentre impostato su 100 120 V lo strumento pu subire seri danni Selezionare un cavo di rete idoneo e collegarne un estremit alla presa di rete dell alimentatore EPS 3501 vedere Fig 2 e l altra ad una presa CA con messa a terra Accendere l unit Ad ogni accensione lo strumento effettua un test di autodiagnosi Se durante il test viene rilevato un errore sul display apparir un messaggio e verr emesso un allarme acustico Collegamento alla e unit per elettroforesi Collegare i cavi dell alimentatore per elettroforesi rosso con rosso e nero o blu con blu Fig 1 vedere prima di copertina Il cavo rosso positivo ed il cavo nero o blu negativo AVVERTENZA Utilizzare esclusivamente cavi elettrici non danneggiati ed attrezzature specificate per la tensione utilizzata Con lo stesso programma possono essere utilizzate due unit per elettroforesi contemporaneamente In questo caso si prega di ricordare di raddoppiare i limiti di corrente e potenza La tensione non varia a seconda del numero di unit utilizzate gt E 5 ZE 3 Disimballaggio ed installazione fe Z lt lt 8 Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD Descrizione tecnica 4 4 Descrizione tecnica 41 Pannello anterior
69. programmazione ed avviamento Nel modo RUN premendo SET si visualizzano anche i parametri programmati per l attuale funzionamento Inoltre il tasto SET permette di cambiare il programma durante il funzionamento dopo aver premuto PAUSE CONTINUE y Change up Change down Consentono di modificare i parametri il valore o un altra opzione nel campo lampeggiante Tenendo premuti i tasti i valori numerici vengono modificati in modo accelerativo Premendo brevemente si cambiano i valori in incrementi pre impostati Le selezioni di parametri unit come Vh e VES NO si cambiano con un unica pressione del tasto tasti possono essere utilizzati anche per commutare tra tempo e voltore nei modi RUN PAUSE ed END valori scorreranno automaticamente cio una volta raggiunto il massimo si passa al minimo o viceversa RUN Premendo RUN si avvia il funzionamento e commuta il programma nel modo RUN Sul display vengono visualizzati il numero del programmi ed i valori attuali di tensione corrente e potenza Vengono anche visualizzati il tempo trascorso o le voltore La commutazione tra tempo e voltore avviene tramite i tasti W l gt gt Z Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD 9 4 Descrizione tecnica PAUSE CONTINUE Commuta lo strumento nel modo PAUSE e disattiva l alimentazione Il display visualizza lo continue stato di funzionamento alla pressione del tasto pause PAUSE CONTINUE
70. r bergeht 4 Manuelle oder automatische Unterbrechung w hlen F r eine automatische Unterbrechung die Zwischenstopparameter in Voltstunden Vh oder in Zeit h w hlen Entweder die korrekte Einheit oder f r eine manuelle Unterbrechung OFF mit 4 1 w hlen Durch Dr cken von ENTER best tigen 5 Wurde Voltstunden oder Zeit gew hlt den f r den Zwischenstopparameter zu verwendenden Wert mit den Tasten J und ENTER einstellen 6 Wurde unter 4 ein automatischer Zwischenstopparameter gew hlt dann wird der Benutzer gefragt ob er m chte daB nach jedem Programmlauf ein Alarm ert nt Mit 4 YES oder NO w hlen und dann ENTER dr cken Wird YES gew hlt dann wird dies durch ein kleines A wie in 1A auf der rechten Seite der Programmnummer in der Anzeige angezeigt 7 Nach abgeschlossener Programmierung sollte das Elektrophoreseger t an die Ausg nge angeschlossen und die Taste RUN gedr ckt werden 8 Eserscheinen die aktuellen Werte von Spannung Strom und Leistung auf der Anzeige zusammen mit dem Wert f r die abgelaufene Zeit oder die Voltstunden Umschalten mit J 1 9 Die Taste PAUSE CONTINUE kann zum Unterbrechen und nachfolgenden Fortsetzen des Programmlaufs verwendet werden 10 Die programmierten Parameter k nnen w hrend des Programmlaufs durch Dr cken von SET berpr ft werden 11 Der Programmlauf stoppt automatisch oder nach dem Dr cken von STOP Electrophoresis Power Supply EPS 3501 Bedienungsan
71. rollate mediante tensione o corrente L EPS 3501 imposta automaticamente i parametri di controllo in conformit ai limiti programmati ed alle variazioni di conduttivit nel sistema gt c 2 Z Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD 3 1 Introduzione fe Z lt lt 4 Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD Informazioni sulla sicurezza 2 2 Informazioni sulla sicurezza 2 1 Norme di sicurezza Poich questo strumento pu sviluppare una tensione ed una corrente sufficienti per provocare una scossa elettrica letale prestare particolare attenzione durante il suo utilizzo Per evitare il rischio di incidenti l alimentatore deve essere utilizzato esclusivamente da personale esperto ed addestrato ed in conformit alle istruzioni del fabbricante Leggere attentamente l intero contenuto del manuale prima di utilizzare l alimentatore 1 Electrophoresis Power Supply EPS 3501 Manuale dell operatore 18 1130 21 Edition AD 5 Questo strumento stato progettato per essere utilizzato solamente in ambienti chiusi Assicurarsi che il conduttore di messa a terra del cavo di alimentazione dello strumento sia collegato correttamente a terra alla presa di rete Per consentire un sufficiente raffreddamento assicurarsi che le bocchette posteriori e laterali dello strumento non siano coperte Non utilizzare lo strumento in
72. ronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 23 ENGLISH 6 Maintenance 24 Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 7 Trouble shooting Trouble shooting 7 If an error that can be corrected by the user occurs either during a run or when switching on the power supply the program enters the HALT mode and the output is switched off Four different type of errors can cause HALT The following list shows the error message on the display the cause and the remedy Error message Cause Remedy HALT Low start current HALT Ground leakage current HALT Mains Voltage too low HALT Mains fail Program stopped The current is less than the lower limit This can be due to incorrect connection of the electrophoresis equipment or due to use of buffers with extremely low conductivity The current to ground leakage in the electrophoresis unit is too high The mains voltage is too low see Specifications Mains power failure for more than 7 s 1 Check connections and or buffers 2 Press MORE switch off the start current check in the MORE mode IMPORTANTI Read Section 5 4 first
73. rop lev e la tension est coup e imm diatement Une r sistance trop lev e peut aussi avoir pour cause l utilisation de tampons conductivit extr mement faible L EPS 3501 coupe la tension dans ce cas et affiche un message d erreur Cette fonction peut tre d sactiv e pour certaines applications voir Figure 4 D sactivation v rification du courant 3 Sudden load change detection D tection d une variation soudaine de charge Cette fonction emp che tout accident pendant l ex cution provoqu par une rupture du circuit lectrique par exemple une connexion d fectueuse du branchement l unit d lectrophor se L EPS 3501 coupe imm diatement la haute tension dans une telle situation 6 Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD Reception et installation 3 3 Reception et installation R ception V rifier le contenu l aide de la liste d inventaire V rifier que le mat riel n a pas t endommag pendant le transport Signaler imm diatement toute d t rioration au repr sentant GE Healthcare local et la soci t de transport en question Branchement sur la tension secteur S lectionner la tension appropri e 100 120 V ou 220 240 V Figure 2 voir verso de la page de couverture d alimentation lectrique est branch e sur la tension secteur de 220 240 V avec une tension secteur s lectionn e sur 100 120 V AVERTISSEMENT Le g n rateur risque d tr
74. s instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Recycling This symbol indicates that the waste of electrical R and electronic equipment must not be disposed as unsorted municipal waste and must be EE collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment This product meets the requirement of the EMC Directive 89 336 EEC through the harmonized standard EN 61 326 1 1997 A1 1998 C
75. s pour basculer entre la dur e et les volt heures en mode RUN ex cution PAUSE et END fin Les valeurs d filent en l occurrence elles changent automatiquement du maximum au minimum ou viceversa set Electrophoresis Power Supply EPS 3501 Manuel d Utilization 18 1130 21 Edition AD 9 FRANCE 4 Description technique 10 pause continue STOP RUN Ex cution Une pression sur la touche RUN d clenche l ex cution du programme et met le programme en mode RUN Le num ro du programme les valeurs effectives de la tension du courant et de la puissance s affichent sur l cran Le temps coul ou le nombre de volt heures s affichent aussi Pour visualiser le temps ou les volt heures utiliser les touches Y f PAUSE CONTINUE Une pression sur cette touche met l instrument en mode PAUSE et coupe la tension L cran d affichage indique les diff rents param tres au moment o l op rateur a appuu sur la touche La touche PAUSE CONTINUE n est active qu en mode RUN Le temps et les valeurs int gr es sont maintenues En mode PAUSE on peut utiliser la touche SET pour effectuer des modifications dans le programme Pour repasser en mode RUN appuyer sur la touche PAUSE CONTINUE ou sur RUN STOP Arr t Une pression sur la touche STOP interrompt l ex cution du programme en cours et met l instrument en mode END Fin La tension est coup e et les param tres de fin de programme sont affich s L ex cution du programme ne pe
76. t 4 S lectionner entre param tres de fin manuel ou automatique Pour le param tre automatique s lectionner les unit s soit en nombre Volt heures Vh soit en dur e h S lectionner l unit appropri e ou si vous pr f rez la version manuelle choisir OFF arr t automatique d sactiv l aide d une des touches Y Confirmer en appuyant sur ENTER 5 Sivous avez s lectionn comme unit les Volt heures ou la dur e entrez la valeur du param tre de fin l aide de l une des touches W et ENTER 6 En cas de s lection param tre de fin automatique p 4 le syst me demande si vous souhaitez une alerte sonore en fin d ex cution du programme Entrez YES oui ou NO non avec Y et appuyez sur ENTER La s lection YES s affiche sous la forme d un A comme dans 1A droite du num ro de programme l cran 7 Lorsque la programmation est termin e brancher l unit d lectrophor se sur les connecteurs de sortie et appuyer sur la touche RUN 8 Latension effective le courant effectif et la puissance effective sont affich s ainsi la dur e coul e ou le nombre de Volt heures basculer d une valeur l autre en appuyant sur l une ou l autre touche WR 9 Utiliser PAUSE CONTINUE pour interrompre et reprendre ensuite le cours d ex cution du programme 10 Les param tres programm s peuvent tre v rifi s pendant l ex cution en appuyant sur la touche SET 11 L Electrophor se ainsi programm e s
77. taining or blotting immediately 5 4 Optional programming You can disable the start current check that otherwise checks that the resistance is not higher than a specified limit This disabling function is set in the MORE mode instead of the SET mode in order not to confuse users who do not need this feature and for safety reasons The MORE mode is described in Fig 5 After pressing MORE the program asks you for the program number and if you want to keep the start current check Choose program number and change between YES and NO by J f and confirm with ENTER Choosing NO for start current check means that the function is disabled and high voltage can be applied despite a very high resistance Leave the MORE mode by EXIT WARNING By disabling the start current check the power supply can deliver high voltage even if it is not connected to electrophoresis equipment AN A Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD 19 ENGLISH 5 Operation 20 1 Enter MORE SEI p2 3000 Vh 2500V 50 mA 30W 2 Select program MORE P2 Select program Select by 0 Select MORE by Confirm by 3 Choose current check no Current check at start YES no Select by Confirm by f 0 Exit by 4 Back to SET mode SER p2 3000 Vh 2500 V 50 mA 30W Fig 5 Disabling the current check 5 5 Choosing run parameters EPS 3501 is an automatic cross over power supply that allows the user
78. tecla PAUSE CONTINUE s lo funciona en modalidad de RUN Se retienen los valores de tiempo e integraci n En la modalidad de pausa la tecla SET puede usarse para introducir modificaciones en el programa Para volver a la modalidad RUN pulsar PAUSE CONTINUE o RUN STOP Interrumpe el funcionamiento y pone el aparato en modalidad END Se desconecta la tensi n y se visualizan los par metros finales Un funcionamiento interrumpido con la tecla STOP no puede continuar MORE Inhabilita el control de la corriente de arranque que detecta si la resistencia es demasiado alta y desconecta la electricidad EXIT Para la ejecuci n de una operaci n como la introducci n de un valor S lo se retienen los valores o unidades ya confirmadas con la tecla ENTER Devuelve el equipo a la modalidad abandonada o a la posici n de arranque en SET pause STOP more exit 4 1 3 Tomas de salida Hay dos tomas de salida para la conexi n y funcionamiento simult neo de dos unidades electrofor ticas la figura 1 ver interior de la cubierta La tensi n saliente es de 0 3500 V El enchufe negativo da entre O y 1750 V y el positivo entre O y 1750 V 4 2 Panel posterior En el panel posterior ilustrado en la figura 2 ver interior de la cubierta hay 1 Un interruptor de red pulsar para conectar la electricidad de la fuente de alimentaci n pulsar 0 para desconectarla 2 Unenchufe para el cable de red 3 Un conmutador de interval
79. to set limits for voltage current and power During electrophoresis only one of the parameters is limiting at a time The limiting parameter determines together with the conductivity in the electrophoresis system the values for the other two parameters Voltage current power and conductivity are related by the following equations U I L 1 P Uxl 2 Where U Voltage Current P Power and L Conductivity Equation 1 is more familiar if the conductivity is replaced by the reciprocal resistance R U RxI Ohm s law The electric field E measured in V cm is the driving force behind electrophoresis E U d where E Electrical field strength U Voltage d distance The electrical field strength is achieved by applying a voltage The higher the voltage the faster the electrophoresis Fast electrophoresis is beneficial since it counteracts diffusion The disadvantage of increasing the voltage too much is that most of the generated electrical energy the product of power and time is transformed to heat Therefore cooling of electrophoresis equipment is recommended Cooling will also reduce smiling effects which are caused by mobility differentials across an electrophoresis gel resulting from poor heat transfer Since the cooling efficiency cannot be increased indefinitely the power should be limited when programming the power supply Electrophoresis Power Supply EPS 3501 User Manual 18 1130 21 Edition AD Operation 5
80. ufs zu dem Zeitpunkt an an dem die Taste gedr ckt wurde PAUSE CONTINUE funktioniert nur im RUN Modus Zeit und Integrationswerte bleiben erhalten Im Pausemodus k nnen durch Dr cken der Taste SET Anderungen am Programm vorgenommen werden Durch Dr cken der Taste PAUSE CONTINUE oder der Taste RUN kann der Benutzer in den RUNModus zur ckkehren STOP Diese Taste unterbricht den Programmlauf und schaltet das Ger t in den END Modus Die Spannung wird abgeschaltet und die Endparameter werden angezeigt Ein Programmlauf kann nach dem Dr cken der Taste STOP nicht fortgesetzt werden MORE Mit dieser Taste kann die Funktion Start Current Check Strompr fung starten die sonst berpr ft ob der Widerstand zu hoch ist und den Strom abschaltet gesperrt werden EXIT Diese Taste stoppt die Ausf hrung eines Vorgangs sowie die Eingabe eines Wertes Es bleiben nur Werte Einheiten erhalten die zuvor bereits mit der Taste ENTER best tigt waren Bringt das Ger t in den vorhergehenden Modus oder in die in SET eingestellte Startposition zur ck 4 1 5 Ausgangsbuchsen Es sind zwei S tze von Ausgangsbuchsen vorhanden so da zwei Elektrophoreseger te angeschlossen und gleichzeitig betrieben werden k nnen Abb 1 siehe Deckblattinnenseite Der Spannungsausgang ist 0 3500 V Die negative Ausgangsbuchse gibt 0 bis 1750 V die positive 0 bis 1750 V 4 2 R ckwand Die R ckwand ist in Abb 2 siehe Deckblattinnenseite dargestellt An d
81. ugaatierra funcional Si la fuente de alimentaci n se conectara a una unidad electrofor tica que tiene fuga a tierra el fallo es detectado y se desconecta la alta tensi n 2 Control de la corriente de arranque Para asegurar la conexi n correcta de la unidad electrofor tica la fuente de alimentaci n controla que la resistencia no supere un l mite especificado en una tensi n de seguridad baja lt 40V Si esta resistencia es demasiado alta la tensi n se desconecta Una resistencia demasiado alta tambi n puede deberse a la utilizaci n de tampones con conductividad excesivamente baja En este caso la alta tensi n tambi n se desconecta y se visualiza un mensaje de error Esta funci n puede inhabilitarse para ciertas aplicaciones ver la figura 4 Inhabilitaci n del control de corriente 3 Detecci n de cambio de carga repentino Esta funci n impide accidentes durante el funcionamiento debidos a ruptura del circuito el ctrico como una mala conexi n a la unidad electrofor tica La alta tensi n se desconecta Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD Desembalaje e instalaci n 3 3 Desembalaje e instalaci n gt gt Electrophoresis Power Supply EPS 3501 Manual del usuario 18 1130 21 Edition AD Desembalaje Controlar la entrega con la lista de contenido adjunta Ver si se han producido da os durante el transporte De haberlos comunicarlo inmediatamente al representante
82. ut pas tre reprise son point d interruption apr s une pression sur la touche Y MORE Suite Une pression sur cette touche permet de d sactiver la mise sous tension apr s v rification du courant qui normalement d tectent si la r sistance est trop lev e et coupe la tension lorsque c est le cas EXIT Sortie Une pression sur cette touche permet d arr ter l ex cution de l op ration en cours comme la saisie d une valeur par exemple Apr s une pression sur cette touche seules les valeurs unit s qui ont d j t confirm es par une pression sur la touche ENTER vont tre gard es De plus le g n rateur est replac dans le mode interrompu ou dans le mode D marrage de la touche SET 4 1 5 Prises connecteurs de sortie L instrument comporte deux jeux de connecteurs pour permettre le branchement et l utilisation simultan e de deux unit s d lectrophor se Figure 1 voir verso de la page de couverture La tension de sortie se situe entre 0 3500 V Le connecteur n gatif est un potentiel compris entre O et 1750 V et le connecteur positif est un potentiel compris entre 0 et 1750 V 4 2 Panneau arri re Le panneau arri re est repr sent sur la Figure 2 voir verso de la page de couverture Sur le panneau arri re sont log s 1 Uncommutateur d alimentation secteur Basculer ce commutateur en position I pour mettre l instrument sous tension Le basculer en position O pour mettre l instrument h

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